Frontiers in Environmental Microbiology

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Application of Different Organic Wastes for Electricity Generation by Means of Double Chambered Microbial Fuel Cell Technology

Received: 29 May 2018    Accepted: 02 July 2018    Published: 30 July 2018
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Abstract

Wastes generated by both agrobased industries and domestic units have high nutrient contents to support microbial growth but in Nigeria, these wastes are indiscriminately dumped and constitutes environmental and health hazards. Some of these wastes can be used to grow some bacterial species in microbial fuel cell to generate bioelectricity. The waste materials used in this work are, banana, and pineapple peels. Glucose was used for comparison. Bacterial species used were isolated from fluids collected from dustbins and soil, all within Chukwuemeka Odumegwu Ojukwu University, Uli campus. Microscopic characterization of the isolates by Grams reaction revealed the isolate to be Gram negative and Spore test were negative. Biochemical tests showed that the isolate were catalase positive, oxidase negative and citrate positive. By Bergys criteria, the isolate was shown to be Pseudomonas species. Genetic characterization confirmed the species to be Pseudomonas aeruginosa. Four (4) MFC’s were fabricated with a liter transparent plastic for the anode and cathode poles. The electrodes used was graphite for the production of graphite-graphite fuel cells. A 3.75% Sodium chloride, 2.2% agar salt bridge connected the chambers. Glucose, banana and pineapple peels were used as organic substrates and pure cultures of Pseudomonas aeruginosa were biocatalyst for the fuel cells. Result for effect of aeration with Pseudomonas aeruginosa by multimetric monitoring gave maximum power of 3.983 X 10-4 W, 7.1625 X 10-4W and 8.9920 X 10-4 W for glucose, banana peel and pineapple peel respectively, while non-aeration was 3.936 X 10-4 W, 7.059 X 10-4 W and 8.909 X 10-4 W for glucose, banana peel and pineapple peel. Population growth determination by spectrophotometric method at wavelength 540 nm gave these results 1.148, 1.572, 1.714 and 1.837 with Pseudomonas aeruginosa on days 1, 4, 7 and 10 respectively.

DOI 10.11648/j.fem.20180404.11
Published in Frontiers in Environmental Microbiology (Volume 4, Issue 4, August 2018)
Page(s) 94-102
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Banana Peel, Multimetric Monitoring, Pineapple Peel, Population Growth Determination, Pseudomonas Aeruginosa, Spectrophotometric Method

References
[1] Adeleye, S. A. and Okorondu, S. I. (2015). Bioelectricity from students’ hostel waste water using microbial fuel cell. International Journal of Biological and Chemical Science 9(2): 1038–1049.
[2] Bond, D. R. and Lovley, D. R. (2003). Electricity production by Geobacter sulfurreducens attached to electrodes. Applied and Environmental Microbiology 69: 1548–1555.
[3] Booki, M., Shaon-cheng and Bruce, E. (2005). Electricity generation using Membrane and Salt bridge microbial fuel cells. Journal of Water Research1675 –1686.
[4] Bruce, E. and Logan, B. E. (2009). Exoelectrogenic bacteria that power microbial fuel cell. Trends Microbiology 375–381.
[5] Chaudhuri, S. K. and Lovley, D. R. (2003). Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cells. Nature Biotechnology 21: 1229–1232.
[6] Cheesbrough, M. (2006). District laboratory practice in tropical countries. 2nd Edn. Cambridge University Press, Cambridge, UK., ISBN-13: 9781139449298.
[7] Cohen, B. (1931). The Bacterial Culture as an Electrical Half-Cell. Thirty-second Annual Meeting of the Society of American Bacteriologists.
[8] Derek R. L. (2008). The Microbe electric: Conversion of organic matter to electricity. Current Opinion in Biotechnology19: 564–571.
[9] Di-yan. (2014). Electricity generation and H2 evolution by microbial fuel cell. Journal of Environmental Sciences110–135.
[10] Du, Z., Li, H. and Gu, T. (2007). A State of the Art review on microbial fuel cells: A promising technology for wastewater treatment and bioenergy. Biotechnology Advances25: 464–482.
[11] Fan, Y., Sharbrough, E. and Liu, H. (2008). Quantification of the internal resistance distribution of microbial fuel cells. Environmental Science & Technology42: 8101–8107.
[12] Fawole, M. O. and B. A. Oso, (2004). Characterization of Bacteria: Laboratory Manual of Microbiology. 4th Edn., Spectrum Book Ltd., Ibadan, Nigeria, pp: 24-33.
[13] Ghangrekar, M. M. and Shinde, V. B. (2006). Wastewater Treatment in Microbial Fuel Cell and Electricity Generation: A Sustainable Approach. Paper presented in the 12th international sustainable development research conference. Hong Kong, April 6-8.
[14] Ghoreyshi, A., Jafary, T., Najafpour, G. D. and Haghparast, F. (2011). Effect of type and concentration of substrate on power generation in a dual chambered microbial fuel cell. Journal of World Renewable Congress 1175–1181.
[15] Gil, G. C., Chang, I. S., Kim, B. H., Kim, M., Jang, J. Y., Park, H. S. and Kim, H. J. (2003). Operational parameters affecting the performance of a mediatorless microbial fuel cell. Biosensensor and Bioelectronics 18: 327–334.
[16] Huang, L., Regan, J. M. and Quan, X. (2011). Electron transfer mechanisms, new applications, and performance of biocathode microbial fuel cells. Bioresource Technology, 10: 316–323.
[17] Ieropoulos, I., Melhuish, C. and Greenman, J. (2003). Artificial metabolism: towards true energetic autonomy in artificial life. Lecture Notes in Computer Science 2801: 792–799.
[18] Ismail, M. and Phadke, R. P. (2014). A PDMS fabricated miniature microbial fuel cell. International Journal of Scientific and Technology Research200–203.
[19] Jang, J. K., Hai, P., Chang, I. S., Kang, K. H., Moon, H., Cho, K. S. and Kima, B. H. (2004) Construction and operation of a novel mediator- and membrane-less microbial fuel cell. Process Biochemistry39: 1007–1012
[20] Jothinathan, D., Sundaram, M. and Wilson, R. (2013). A study of bioelectricity production by the synergistic action of Bacillus tequilensis and Pseudomonas aeruginosa isolated from rumen fluid. American Journal of Environmental Sciences9: 424–430.
[21] Kim, H. J., Park, H. S., Hyun, M. S., Chang, I. S, Kim, M. and Kim, B. H. (2002). A mediatorless microbial fuel cell using a metal reducing Bacterium, Shewanella putrefaciens. Enzyme and Microbial Technology 30: 14–152.
[22] Kun, Daniel, J., Hassell and Tingyue, J. (2012). Microbial fuel cell: Electricity generation from organic waste by microbes. In R. Arora (Ed.) Microbial Biotechnology: Energy and environment. (Pp. 162–189). United Kingdom: CAB International, Oxon press.
[23] Logan, B. E. (2006). Electricity-Producing bacterial communities in microbial fuel cells. Trends in Microbiology 14: 512–518.
[24] McKellar, R. and Lu, X. (2004). Modeling Microbial Responses on Foods. CRC Press: Boca Raton, FL.
[25] Min, B., Kim, J. R., Oh, S. E., Regan, J. M. and Logan, B. E. (2005). Electricity generation from swine wastewater using microbial fuel cells. Water Research 3: 4961 – 4968.
[26] Mokhatarian, Rshimnejad, M. G. D., Najafpour and Ghoreyshi, B. (2012). Effect of different substrate on performance of microbial fuel cell. African Journal of Biotechnology3363 – 3369.
[27] Moon, H., Chang, I. S. and Kim, B. H. (2006). Continuous Electricity production from artificial wastewater using a Mediator-less Microbial Fuel Cell. Bioresource Technology 97: 621–627.
[28] Nelli and Beecroft. (2010). Development of microbial community Dynamics. American Journal of Environmental Science201–263.
[29] Olutiola, P. O., Famurewa, O. and Sonntag, H. G. (2000). Introduction to General Microbiology: A Practical Approach. 2nd Edn., Bolabay Publications, Ikeja, Nigeria.
[30] Park, D. H. and Zeikus, J. G. (2003). Improved fuel cell and electrode designs for producing electricity from microbial degradation. Biotechnology and Bioengineering 81: 348–355.
[31] Peleg, M. (2006). Advanced Quantitative Microbiology for Food and Biosystems: Grades of Graphite. Journal of Green Engineering4: 13–32.
[32] Pranab, K. and Deka, D. (2010). Electricity generation from bio waste based microbial fuel cells. International Journal of Energy, Information and Communications77– 92.
[33] Potter, M. C. (1911). Electrical Effects Accompanying the Decomposition of Organic Compounds. Royal Society of London 84(571): 260–276.
[34] Rabaey, K. and Verstraete, W. (2005). Microbial fuel cells: Novel Biotechnology for energy generation. Trends Biotechnology23: 291–298.
[35] Rakesh, Chandra, Ujwal, S. M. and Suresh, R. (2014). Performance Studies on Microbial fuel cell. International Journal of Research in Engineering and Technology169–173.
[36] Reddy, N., Nirmal R. K., Ajay, B. O. and Muralidharan, K. (2007). A Potential stage in wastewater treatment for generation of bioelectricity using MFC. Current Research Topics in Applied Microbiology and Microbial Biotechnology 1: 322326.
[37] Rozendal, R., Hamelers, H. V., Rabaey, K., Keller, J. and Buisman, C. J. (2008). Towards practical implementation of bioelectrochemical wastewater treatment. Trends in Biotechnology 26: 450–459.
[38] Saravanakumari, P. and Angel, D. (2015). Two chamber microbial fuel cells for electricity generation using different carbon sources. British Microbiology Research Journal 5(1): 12–21.
[39] Shafiee, S. and Topal, E. (2009). When will fossil fuel reserves be diminished? Energy Policy 37: 181–189.
[40] Singh, D., Pratap, Y., Baranwal, B., Kumar and Chauddhary, R. K. (2010). Microbial fuel cells: A Green technology for power generation. Annals of Biological Research128–138.
[41] Wang, X., Feng, Y., Z. and Hao, Q. (2009). Accelerated start-up of two chambered microbial fuel cells; effect of positive poised potential. Journal of Electrochemical Activity54:1109–1114.
[42] Wei, J., Liang, P. and Huang, X., (2011). Recent progress in electrodes for microbial fuel cells. Bioresources Technology11:1–47.
[43] Yongtae A, Marta, C. Hatzell F. Z., and Logan B. E. (2014). Different electrode configurations to optimize performance of multielectrode microbial fuel cells for generating power or treating domestic wastewater. Journal of Power Sources249: 440–445.
Author Information
  • Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Nigeria

  • Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Uli, Nigeria

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    Okeke Ugochukwu Chibueze, Mbachu Ifeoma Adaora Chima. (2018). Application of Different Organic Wastes for Electricity Generation by Means of Double Chambered Microbial Fuel Cell Technology. Frontiers in Environmental Microbiology, 4(4), 94-102. https://doi.org/10.11648/j.fem.20180404.11

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    Okeke Ugochukwu Chibueze; Mbachu Ifeoma Adaora Chima. Application of Different Organic Wastes for Electricity Generation by Means of Double Chambered Microbial Fuel Cell Technology. Front. Environ. Microbiol. 2018, 4(4), 94-102. doi: 10.11648/j.fem.20180404.11

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    Okeke Ugochukwu Chibueze, Mbachu Ifeoma Adaora Chima. Application of Different Organic Wastes for Electricity Generation by Means of Double Chambered Microbial Fuel Cell Technology. Front Environ Microbiol. 2018;4(4):94-102. doi: 10.11648/j.fem.20180404.11

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  • @article{10.11648/j.fem.20180404.11,
      author = {Okeke Ugochukwu Chibueze and Mbachu Ifeoma Adaora Chima},
      title = {Application of Different Organic Wastes for Electricity Generation by Means of Double Chambered Microbial Fuel Cell Technology},
      journal = {Frontiers in Environmental Microbiology},
      volume = {4},
      number = {4},
      pages = {94-102},
      doi = {10.11648/j.fem.20180404.11},
      url = {https://doi.org/10.11648/j.fem.20180404.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.fem.20180404.11},
      abstract = {Wastes generated by both agrobased industries and domestic units have high nutrient contents to support microbial growth but in Nigeria, these wastes are indiscriminately dumped and constitutes environmental and health hazards. Some of these wastes can be used to grow some bacterial species in microbial fuel cell to generate bioelectricity. The waste materials used in this work are, banana, and pineapple peels. Glucose was used for comparison. Bacterial species used were isolated from fluids collected from dustbins and soil, all within Chukwuemeka Odumegwu Ojukwu University, Uli campus. Microscopic characterization of the isolates by Grams reaction revealed the isolate to be Gram negative and Spore test were negative. Biochemical tests showed that the isolate were catalase positive, oxidase negative and citrate positive. By Bergys criteria, the isolate was shown to be Pseudomonas species. Genetic characterization confirmed the species to be Pseudomonas aeruginosa. Four (4) MFC’s were fabricated with a liter transparent plastic for the anode and cathode poles. The electrodes used was graphite for the production of graphite-graphite fuel cells. A 3.75% Sodium chloride, 2.2% agar salt bridge connected the chambers. Glucose, banana and pineapple peels were used as organic substrates and pure cultures of Pseudomonas aeruginosa were biocatalyst for the fuel cells. Result for effect of aeration with Pseudomonas aeruginosa by multimetric monitoring gave maximum power of 3.983 X 10-4 W, 7.1625 X 10-4W and 8.9920 X 10-4 W for glucose, banana peel and pineapple peel respectively, while non-aeration was 3.936 X 10-4 W, 7.059 X 10-4 W and 8.909 X 10-4 W for glucose, banana peel and pineapple peel. Population growth determination by spectrophotometric method at wavelength 540 nm gave these results 1.148, 1.572, 1.714 and 1.837 with Pseudomonas aeruginosa on days 1, 4, 7 and 10 respectively.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Application of Different Organic Wastes for Electricity Generation by Means of Double Chambered Microbial Fuel Cell Technology
    AU  - Okeke Ugochukwu Chibueze
    AU  - Mbachu Ifeoma Adaora Chima
    Y1  - 2018/07/30
    PY  - 2018
    N1  - https://doi.org/10.11648/j.fem.20180404.11
    DO  - 10.11648/j.fem.20180404.11
    T2  - Frontiers in Environmental Microbiology
    JF  - Frontiers in Environmental Microbiology
    JO  - Frontiers in Environmental Microbiology
    SP  - 94
    EP  - 102
    PB  - Science Publishing Group
    SN  - 2469-8067
    UR  - https://doi.org/10.11648/j.fem.20180404.11
    AB  - Wastes generated by both agrobased industries and domestic units have high nutrient contents to support microbial growth but in Nigeria, these wastes are indiscriminately dumped and constitutes environmental and health hazards. Some of these wastes can be used to grow some bacterial species in microbial fuel cell to generate bioelectricity. The waste materials used in this work are, banana, and pineapple peels. Glucose was used for comparison. Bacterial species used were isolated from fluids collected from dustbins and soil, all within Chukwuemeka Odumegwu Ojukwu University, Uli campus. Microscopic characterization of the isolates by Grams reaction revealed the isolate to be Gram negative and Spore test were negative. Biochemical tests showed that the isolate were catalase positive, oxidase negative and citrate positive. By Bergys criteria, the isolate was shown to be Pseudomonas species. Genetic characterization confirmed the species to be Pseudomonas aeruginosa. Four (4) MFC’s were fabricated with a liter transparent plastic for the anode and cathode poles. The electrodes used was graphite for the production of graphite-graphite fuel cells. A 3.75% Sodium chloride, 2.2% agar salt bridge connected the chambers. Glucose, banana and pineapple peels were used as organic substrates and pure cultures of Pseudomonas aeruginosa were biocatalyst for the fuel cells. Result for effect of aeration with Pseudomonas aeruginosa by multimetric monitoring gave maximum power of 3.983 X 10-4 W, 7.1625 X 10-4W and 8.9920 X 10-4 W for glucose, banana peel and pineapple peel respectively, while non-aeration was 3.936 X 10-4 W, 7.059 X 10-4 W and 8.909 X 10-4 W for glucose, banana peel and pineapple peel. Population growth determination by spectrophotometric method at wavelength 540 nm gave these results 1.148, 1.572, 1.714 and 1.837 with Pseudomonas aeruginosa on days 1, 4, 7 and 10 respectively.
    VL  - 4
    IS  - 4
    ER  - 

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