Antimicrobials Resistance of Bacteria Isolated from Three Bathing Waters in Southern Côte d'Ivoire
Frontiers in Environmental Microbiology
Volume 5, Issue 4, August 2019, Pages: 92-99
Received: Oct. 2, 2019; Accepted: Oct. 21, 2019; Published: Oct. 25, 2019
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Authors
Thérèse Kouassi-Agbessi, Laboratory of Water and Food Analysis, National Institute of Public Health, Abidjan, Côte d’Ivoire; Department of Pharmaceutical and Biological Sciences, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire
Timothée Ouassa, Department of Pharmaceutical and Biological Sciences, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire; Center for Diagnosis and Research on AIDS, University Hospital Center of Treichville, Abidjan, Côte d’Ivoire
Anderson Richmond Djatchi, Department of Pharmaceutical and Biological Sciences, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire; Center for Diagnosis and Research on AIDS, University Hospital Center of Treichville, Abidjan, Côte d’Ivoire
Yessé Nanga Zinzendorf, Department of Pharmaceutical and Biological Sciences, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire; Laboratory of Microbiology, National Laboratory of Public Health, Abidjan, Côte d’Ivoire
Aubin Tchapé Gbagbo, Laboratory of Water and Food Analysis, National Institute of Public Health, Abidjan, Côte d’Ivoire
Christophe N’cho Amin, Laboratory of Water and Food Analysis, National Institute of Public Health, Abidjan, Côte d’Ivoire; Department of Pharmaceutical and Biological Sciences, Félix Houphouët-Boigny University, Abidjan, Côte d’Ivoire
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Abstract
Waters used for recreational activities in order to ensure the health of populations who practice their recreational activity. Disease or infection risk associated with recreational water areas is mainly related either to faecal contamination or to domestic or hospital effluents. The present study aimed assessment of microbiological contamination of surface waters (rivers and lake) of three cities in southern Côte d’Ivoire used for recreational activities, by bacteria generally found in humans and animal’s digestive tract, and then assesses their resistance to commonly antimicrobial drugs used in human therapy. Water samples have been taken from different identified sites over a 13-months period. A microbiological analysis based on numbering of germs was performed then isolated strains were assayed for antibiotic sensitivity tests. Data analysis was performed using SPSS 22.0 software. Results have shown the presence of Escherichia coli, Pseudomonas aeruginosa and Enterococcus faecalis in waters analysed. E. coli, a faecal contamination marker, was present in all the analysed waters with levels higher than standards defined by the 2006/7 /EC European Directive for bathing water, making them unsuitable for swimming. E. coli strains showed high levels of resistance to amoxicillin, ticarcillin, nalidixic acid and cotrimoxazole. Moreover, a beta-lactamase producing strain was isolated, suggesting probability of contamination by hospital or domestic effluents. The results of this study show the importance of microbiological monitoring of surface.
Keywords
Antimicrobial Resistance, Bathing Waters, Faecal Contamination, Côte d’Ivoire
To cite this article
Thérèse Kouassi-Agbessi, Timothée Ouassa, Anderson Richmond Djatchi, Yessé Nanga Zinzendorf, Aubin Tchapé Gbagbo, Christophe N’cho Amin, Antimicrobials Resistance of Bacteria Isolated from Three Bathing Waters in Southern Côte d'Ivoire, Frontiers in Environmental Microbiology. Vol. 5, No. 4, 2019, pp. 92-99. doi: 10.11648/j.fem.20190504.12
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Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Pond K. Water recreation and disease: plausibility of associated infections: acute effects, sequelae, and mortality. World Health Organization; 2005.
[2]
George I, Servais P. Sources and dynamics of coliforms in the Seine basin: Summary report. 2002.
[3]
Chee-Sanford JC, Aminov RI, Krapac I, Garrigues-Jeanjean N, Mackie RI. Occurrence and diversity of tetracycline resistance genes in lagoons and groundwater underlying two swine production facilities. Appl Environ Microbiol. 2001; 67 (4): 1494502.
[4]
Volkmann H, Schwartz T, Bischoff P, Kirchen S, Obst U. Detection of clinically relevant antibiotic-resistance genes in municipal wastewater using real-time PCR (TaqMan). Journal of microbiological methods. 2004; 56 (2): 27786.
[5]
Sayah RS, Kaneene JB, Johnson Y, Miller R. Patterns of antimicrobial resistance observed in Escherichia coli isolates obtained from domestic-and wild-animal fecal samples, human septage, and surface water. Appl Environ Microbiol. 2005; 71 (3): 1394404.
[6]
Antunes P, Machado J, Peixe L. Characterization of antimicrobial resistance and class 1 and 2 integrons in Salmonella enterica isolates from different sources in Portugal. Journal of Antimicrobial Chemotherapy. 2006; 58 (2): 297304.
[7]
Messi P, Guerrieri E, De Niederhaeusern S, Sabia C, Bondi M. Vancomycin-resistant enterococci (VRE) in meat and environmental samples. International journal of food microbiology. 2006; 107 (2): 21822.
[8]
Hamelin K, Bruant G, El-Shaarawi A, Hill S, Edge TA, Fairbrother J, Harel J, Maynard C, Masson L, Brousseau R. Occurrence of virulence and antimicrobial resistance genes in Escherichia coli isolates from different aquatic ecosystems within the St. Clair River and Detroit River areas. Appl Environ Microbiol. 2007; 73 (2): 47784.
[9]
Sidrach-Cardona R, Hijosa-Valsero M, Marti E, Balcázar JL, Becares E. Prevalence of antibiotic-resistant fecal bacteria in a river impacted by both an antibiotic production plant and urban treated discharges. Science of the Total Environment. 2014; 488: 2207.
[10]
Yala J-F, Mabika RM, Boucka DB, Lepengue AN, Souza A. Phenotypic study of the resistance of bacteria isolated from lakes and rivers of the city of Franceville to third-generation cephalosporins. Journal of Applied Biosciences. 2017; 119 (1).
[11]
Galvin S, Boyle F, Hickey P, Vellinga A, Morris D, Cormican M. Enumeration and characterization of antimicrobial-resistant Escherichia coli bacteria in effluent from municipal, hospital, and secondary treatment facility sources. Appl Environ Microbiol. 2010; 76 (14): 47729.
[12]
Guessennd N, Ouattara M, Ouattara N, Nevry R, Gbonon V, Tiekoura K, Dosso M, Ger B. Study of Multiresistant Bacteria in Hospital Effluents of a Hospital and University Center (CHU) in the City of Abidjan (Côte d’Ivoire). Journal of Applied Biosciences. 2013; 69: 545664.
[13]
Cablan MA, Nanga YZ, Kouassi-Agbessi T, Loukou YG, Kacou-N’douba A, Dosso M. Microbiological control of different effluents from the University Hospital Center of Treichville. [SPB]: FHB; 2015.
[14]
Ahoussi KE, Koffi YB, Kouassi AM, Soro G, Soro N, Biémi J. Study of the chemical and microbiological characteristics of the water resources of the N'zi watershed: case of the commune of N'zianouan (southern Côte d'Ivoire). International Journal of Biological and Chemical Sciences. 2012; 6 (4): 185473.
[15]
Sadat A, N’goran EBZ, Siaka S, Parinet B. Interest of the multidimensional analysis for the evaluation of the physicochemical quality of the water of a tropical lacustrine system: case of the Yamoussoukro lakes (Côte d’Ivoire). Journal of Applied Biosciences. 2011; 38: 257385.
[16]
Coulibaly A, Mondé S, Wognin V, Aka K. State of anthropic pollution in the estuary of Ebrié lagoon (Côte d’Ivoire) by analysis of the metal elements traces. European Journal of Scientific Research. 2008; 19 (2): 37290.
[17]
Amin NC, Agbessi T, Gbassi K, Dosso V, Kpaibe SP, Kouadio L. Quality control of the bathing water of the Cascade de Man in Ivory Coast. Journal of Scientific Research of the University of Lome. 2017; 19 (4): 8590.
[18]
APHA (2005) Standard Methods for the Examination of Water and Wastewater. 21st Edition, American Public Health Association/American Water Works Association/Water Environment Federation, Washington DC.-References-Scientific Research Publishing [Internet]. [cité 26 sept 2019]. Disponible sur: https://www.scirp.org/(S (czeh2tfqyw2orz553k1w0r45))/reference/ReferencesPapers.aspx?ReferenceID=1870039.
[19]
Mwanamoki PM, Devarajan N, Thevenon F, Atibu EK, Tshibanda JB, Ngelinkoto P, Mpiana PT, Prabakar K, Mubedi JI, Kabele CG. Assessment of pathogenic bacteria in water and sediment from a water reservoir under tropical conditions (Lake Ma Vallée), Kinshasa Democratic Republic of Congo. Environmental monitoring and assessment. 2014; 186 (10): 682130.
[20]
Adingra A, Sako A, Kouassi A. Bacteriological quality of surface waters of Banco River, Abidjan, Côte d’Ivoire. 2010.
[21]
Haller L, Poté J, Loizeau J-L, Wildi W. Distribution and survival of faecal indicator bacteria in the sediments of the Bay of Vidy, Lake Geneva, Switzerland. Ecological indicators. 2009; 9 (3): 5407.
[22]
Tirodimos I, Christoforidou EP, Nikolaidou S, Arvanitidou M. Bacteriological quality of swimming pool and spa water in northern Greece during 2011–2016: is it time for Pseudomonas aeruginosa to be included in Greek regulation? Water Science and Technology: Water Supply. 2018; 18 (6): 193745.
[23]
Jaiani E, Kokashvili T, Mitaishvili N, Elbakidze T, Janelidze N, Lashkhi N, Kalandadze R, Mikashavidze E, Natroshvili G, Whitehouse CA. Microbial water quality of recreational lakes near Tbilisi, Georgia. Journal of water and health. 2013; 11 (2): 33345.
[24]
Schwartz T, Kohnen W, Jansen B, Obst U. Detection of antibiotic-resistant bacteria and their resistance genes in wastewater, surface water, and drinking water biofilms. FEMS microbiology ecology. 2003; 43 (3): 32535.
[25]
Munoz-Aguayo J, Lang KS, LaPara TM, Gonzalez G, Singer RS. Evaluating the Effects of Chlortetracycline on the Proliferation of Antibiotic-Resistant Bacteria in a Simulated River Water Ecosystem. Applied and Environmental Microbiology. 1 sept 2007; 73 (17): 54215.
[26]
Kumarasamy KK, Toleman MA, Walsh TR, Bagaria J, Butt F, Balakrishnan R, Chaudhary U, Doumith M, Giske CG, Irfan S, Krishnan P, Kumar AV, Maharjan S, Mushtaq S, Noorie T, Paterson DL, Pearson A, Perry C, Pike R, Rao B, Ray U, Sarma JB, Sharma M, Sheridan E, Thirunarayan MA, Turton J, Upadhyay S, Warner M, Welfare W, Livermore DM, Woodford N. Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. Lancet Infect Dis. sept 2010; 10 (9): 597602.
[27]
Pharmacology/H. P. Rang [and others] ; illustrations by Peter Lamb.-Version details-Trove [Internet]. [cité 26 sept 2019]. Disponible sur: https://trove.nla.gov.au/work/25740167?q&versionId=45535332.
[28]
Densities and antimicrobial resistance of Escherichia coli isolated from marine waters and beach sands.-PubMed-NCBI [Internet]. [cité 26 sept 2019]. Disponible sur: https://www.ncbi.nlm.nih.gov/pubmed/25963763
[29]
Weiss K. Bacterial resistance: the new cold war. The Doctor of Quebec 37 (3). 2002; 419.
[30]
Drieux-Rouzet L, Jarlier V. Multiresistant bacteria in water: models of enterobacteria producing extended spectrum beta-lactamase. Francophone Journal of Laboratories. 2014; 2014 (460): 759.
[31]
Harris S, Morris C, Morris D, Cormican M, Cummins E. Antimicrobial resistant Escherichia coli in the municipal wastewater system: effect of hospital effluent and environmental fate. Science of the Total Environment. 2014; 468: 107885.
[32]
Aboulfotoh Hashish NM, Gawad Abbass AA, Khamis Amine AE. Pseudomonas aeruginosa in swimming pools. Cogent Environmental Science. 2017; 3 (1): 1328841.
[33]
Klare I, Konstabel C, Badstübner D, Werner G, Witte W. Occurrence and spread of antibiotic resistances in Enterococcus faecium. International journal of food microbiology. 2003; 88 (23): 26990.
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