Immunomodulatory Potential of New Classical Herbomineral Formulation in Experimental Animals: Impact of Biofield Energy Healing Treatment
Advances in Applied Physiology
Volume 2, Issue 1, October 2017, Pages: 18-27
Received: Oct. 30, 2017; Accepted: Nov. 21, 2017; Published: Dec. 5, 2017
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Authors
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, USA
Alice Branton, Trivedi Global, Inc., Henderson, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, USA
Alan Joseph Balmer, Trivedi Global, Inc., Henderson, USA
Dimitrius Anagnos, Trivedi Global, Inc., Henderson, USA
Janice Patricia Kinney, Trivedi Global, Inc., Henderson, USA
Joni Marie Holling, Trivedi Global, Inc., Henderson, USA
Joy Angevin Balmer, Trivedi Global, Inc., Henderson, USA
Lauree Ann Duprey-Reed, Trivedi Global, Inc., Henderson, USA
Vaibhav Rajan Parulkar, Trivedi Global, Inc., Henderson, USA
Mayank Gangwar, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India
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Abstract
A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The aim of the study was to evaluate the immunomodulatory potential of Consciousness Energy Healing Treatment on the herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. Additionally, one group of animals also received Biofield Energy Treatment per se (day -15) by Biofield Energy Healers under similar conditions. The test formulation was evaluated for immunological parameters viz. humoral immune analysis, paw volume, hematological study, biochemistry, body weight, feed and water intake, and histopathology analysis were performed. The humoral immune response showed a significant increased primary and secondary antibody titre values by 26% and 150.4%, respectively in Biofield Energy Treatment per se group (G6, day -15), while primary and secondary antibody titre values were increased by 100.0% and 110.4%, respectively in the Biofield Energy Treated test formulation (G7, day -15) group compared with the disease control group (G2). The results of delayed type hypersensitivity showed a significant increase in the paw volume by 94.44% in the Biofield Energy Treated test formulation (G4) group with respect to the G2 group. However, a significant increase in paw volume by 61.11% and 44.44% was found in G6 and G7 group, respectively with respect to the G2 group. Hematological parameters showed a significant (p<0.001) increased platelet count by 85.6% in the G4 group, while 39.15% and 35.22% increase in the G6 and G7 groups, respectively compared with the G2 group. In biochemical analysis, a significant increase in calcium and phosphorus level were found in the G4 group compared with the G2 group. Animal weight parameters suggests that there were no treatment-related changes in any group, organ to body weight ratio, feed and water intake. The data described that the Biofield Energy Treated test formulation was found to be safe without any side effect during the course of the experiment. These data suggests that the Biofield Energy Treated test formulation and Biofield Energy Treatment per se can be used for autoimmune and inflammatory diseases, enhancing stress management and prevention, and anti-aging by improving overall health.
Keywords
Biofield Energy Healers, The Trivedi Effect®, Autoimmunity, Ant-aging, Alzheimer’s Disease
To cite this article
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Alan Joseph Balmer, Dimitrius Anagnos, Janice Patricia Kinney, Joni Marie Holling, Joy Angevin Balmer, Lauree Ann Duprey-Reed, Vaibhav Rajan Parulkar, Mayank Gangwar, Snehasis Jana, Immunomodulatory Potential of New Classical Herbomineral Formulation in Experimental Animals: Impact of Biofield Energy Healing Treatment, Advances in Applied Physiology. Vol. 2, No. 1, 2017, pp. 18-27. doi: 10.11648/j.aap.20170201.13
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Atal CK, Sharma ML, Kaul A, Khajuria A (1986) Agents of plant origin I: Preliminary screening. J Ethanopharmacol 8: 133-141.
[2]
Gillette NA, Chan C (2000) Application of immunohistochemistry in the evaluation of immunosuppressive agents. Hum Exp Toxicol 19: 251- 254.
[3]
Lake DF, Briggs AD, Akporiayes ET (2007) Basic & clinical pharmacology 11th edition. Singapore & Mc GrawHill companies, LANGE publication, Chapter 56, p 908.
[4]
Marshall SE (2006) Davidson’s Principle of Practice of medicine 20th edition, Chapter 4, p 64, 80.
[5]
Dua PR, Shankar G, Srimal RC, Saxena KC, Saxena RP, Puri A, Dhawan BN, Shukla YN, Thakur RS, Husain A (1989) Adaptogenic activity of Indian Panax pseudoginseng. Indian J Exp Bio 27: 631-634.
[6]
Rishton GM (2008) Natural products as a robust source of new drugs and drug leads: Past successes and present day issues. Am J Cardiol 101: 43D-49D.
[7]
Modak M, Dixit P, Londhe J, Ghaskadbi S, Devasagayam TPA (2007) Indian herbs and herbal drugs used for the treatment of diabetes. J Clin Biochem Nutr 40: 163-173.
[8]
Cragg GM, Newman DJ (2013) Natural products: A continuing source of novel drug leads. Biochimica et Biophysica Acta 1830: 3670-3695.
[9]
Girdhari L, Rana A (2007) Withania somnifera (Ashwagandha): A review. Pharmacogn Rev 1: 129-136.
[10]
Ziauddin M, Phansalkar N, Patki P, Diwanay S, Patwardhan B (1996) Studies on the immunomodulatory effects of ashwagandha. J Ethnopharmacol 50: 69-76.
[11]
Singh N, Bhalla M, de Jager P, Gilca M (2011) An overview on ashwagandha: A rasayana (Rejuvenator) of Ayurveda. Afr J Tradit Complement Altern Med 8: 208-213.
[12]
Lukác N, Massányi P (2007) Effects of trace elements on the immune system. Epidemiol Mikrobiol Imunol 56: 3-9.
[13]
Mazumder PM, Pattnayak S, Parvani H, Sasmal D, Rathinavelusamy P (2012) Evaluation of immunomodulatory activity of Glycyrhiza glabra L roots in combination with zing. Asian Pac J Trop Biomed 2: S15-S20.
[14]
Lutgendorf SK, Mullen-Houser E, Russell D, Degeest K, Jacobson G, Hart L, Bender D, Anderson B, Buekers TE, Goodheart MJ, Antoni MH, Sood AK, Lubaroff DM (2010) Preservation of immune function in cervical cancer patients during chemoradiation using a novel integrative approach. Brain Behav Immun 24: 1231-1240.
[15]
Ironson G, Field T, Scafidi F (1996) Massage therapy is associated with enhancement of the immune system's cytotoxic capacity. Int J Neurosci 84: 205-217.
[16]
Jain S, Hammerschlag R, Mills P, Cohen L, Krieger R, Vieten C, Lutgendorf S (2015) Clinical studies of biofield therapies: Summary, methodological challenges, and recommendations. Glob Adv Health Med 4: 58-66.
[17]
Rubik B (2002) The biofield hypothesis: Its biophysical basis and role in medicine. J Altern Complement Med 8: 703-717.
[18]
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol 4: 141.
[19]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Antimicrobial sensitivity, biochemical characteristics and biotyping of Staphylococcus saprophyticus: An impact of biofield energy treatment. J Women’s Health Care 4: 271.
[20]
Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, Mondal SC, Jana S (2015) Effect of biofield energy treatment on Streptococcus group B: A postpartum pathogen. J Microb Biochem Technol 7: 269-273.
[21]
Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Effect of biofield treatment on antimicrobials susceptibility pattern of Acinetobacter baumannii - An Experimental Study. J Clin Diagn Res 3: 1.
[22]
Trivedi MK, Branton A, Trivedi D, Nayak G, Bairwa K, Jana S (2015) Effect of biofield treatment on physical, thermal, and spectral properties of SFRE 199-1 mammalian cell culture medium. Advances in Biochemistry 3: 77-85.
[23]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of antibiogram, genotype and phylogenetic analysis of biofield treated Nocardia otitidis. Biol Syst Open Access 4: 143.
[24]
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Antibiogram, biochemical reactions, and genotypic pattern of biofield treated Pseudomonas aeruginosa. J Trop Dis 4: 181.
[25]
Trivedi MK, Branton A, Trivedi D, Shettigar H, Bairwa K, Jana S (2015) Fourier transform infrared and ultraviolet-visible spectroscopic characterization of biofield treated salicylic acid and sparfloxacin. Nat Prod Chem Res 3: 186.
[26]
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Effect of biofield treatment on spectral properties of paracetamol and piroxicam. Chem Sci J 6: 98.
[27]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Potential impact of biofield treatment on atomic and physical characteristics of magnesium. Vitam Miner 3: 129.
[28]
Trivedi MK, Branton A, Trivedi D, Nayak G, Panda P, Jana S (2016) Gas chromatography-mass spectrometric analysis of isotopic abundance of 13C, 2H, and 18O in biofield energy treated p-tertiary butylphenol (PTBP). American Journal of Chemical Engineering 4: 78-86.
[29]
Trivedi MK, Branton A, Trivedi D, Nayak G, Sethi KK, Jana S (2016) Gas chromatography-mass spectrometry based isotopic abundance ratio analysis of biofield energy treated methyl-2-napthylether (Nerolin). American Journal of Physical Chemistry 5: 80-86.
[30]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of biochemical marker - glutathione and DNA fingerprinting of biofield energy treated Oryza sativa. American Journal of BioScience 3: 243-248.
[31]
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2016) Molecular analysis of biofield treated eggplant and watermelon crops. Adv Crop Sci Tech 4: 208.
[32]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Morphological characterization, quality, yield and DNA fingerprinting of biofield energy treated alphonso mango (Mangifera indica L.). Journal of Food and Nutrition Sciences 3: 245-250.
[33]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Characterization of physical and structural properties of aluminum carbide powder: Impact of biofield treatment. J Aeronaut Aerospace Eng 4: 142.
[34]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) The potential impact of biofield energy treatment on the atomic and physical properties of antimony tin oxide nanopowder. American Journal of Optics and Photonics 3: 123-128.
[35]
Trivedi MK, Patil S, Tallapragada RM (2013) Effect of bio field treatment on the physical and thermal characteristics of vanadium pentoxide powders. J Material Sci Eng S 11: 001.
[36]
Ladics GS (2007) Primary immune response to sheep red blood cells (SRBC) as the conventional T-cell dependent antibody response (TDAR) test. J Immunotoxicol 4: 149-152.
[37]
Joharapurkar AA, Zambad SP, Wanjari MM, Umathe SN (2003) In vivo evaluation of antioxidant activity of alcoholic extract of Rubia cordifolia Linn. And its influence on ethanol-induced immunosuppression. Indian J Pharmacol 35: 232-236.
[38]
Feldman BF, Zinkl JG, Jain VC (2000) Laboratory techniques for avian hematology,” in Schalm’s Veterinary Hematology, (5thedn) Lippincott Williams & Wilkins, Toronto, Canada.
[39]
Chanda S, Dave R, Kaneria M, Shukla V (2012) Acute oral toxicity of Polyalthia longifolia var. pendula leaf extract in wistar albino rats. Pharmaceutical Biol 50: 1408-1415.
[40]
OECD (1992) OECD Guideline for Testing of Chemicals, vol. 420, Organization for Economic Cooperation and Development, Paris, France.
[41]
Goldman AS, Prabhakar BS. Immunology Overview. In: Baron S, editor. Medical Microbiology. 4th edition. Galveston (TX): University of Texas Medical Branch at Galveston; 1996. Chapter 1. Available from: http://www.ncbi.nlm.nih.gov/books/NBK7795/
[42]
Dean JH, Murray MJ (1993) Toxic responses of the immune system. In Casarett and Doull’s Toxicology. (4thedn), Amdur MO, Doull J, Klaassen CD. New York: McGraw-Hill; p. 282-286.
[43]
Naik SR, Gavankar C, Thakare VN (2015) Immunomodulatory activity of Withina somnifera and Curcuma longa in animal models: Modulation of cytokines functioning. Pharmacologia 6: 168-177.
[44]
Nagalakshmi D, Sridhar K, Parashuramulu S (2015) Replacement of inorganic zinc with lower levels of organic zinc (zinc nicotinate) on performance, hematological and serum biochemical constituents, antioxidants status and immune responses in rats, Veterinary World 8: 1156-1162.
[45]
Ziauddin M, Phansalkar N, Patki P, Diwanay S, Patwardhan B (1996) Studies on the immunomodulatory effects of ashwagandha. J Ethnopharmacol 50: 69-76.
[46]
Agarwal R, Diwanay S, Patki P, Patwardhan B (1999) Studies on immunomodulatory activity of Withania somnifera (ashwagandha) extracts in experimental immune inflammation. J Ethnopharmacol 67: 27-35.
[47]
Owais M, Sharad KS, Shehbaz A, Saleemuddin M (2005) Antibacterial efficacy of Withania somnifera (ashwagandha) an indigenous medicinal plant against experimental murine salmonellosis. Phytomedicine 12: 229-235.
[48]
Qayyum R, Kurbanova N, Zia R, Adomaityte J (2014) Serum selenium levels are associated with blood platelet count in US Adults. Meeting: SHM Annual Meeting 2014.
[49]
Liu L, Li N, Lei T, Li K, Zhang Y (2014) The in vitro biological properties of Mg-Zn-Sr alloy and superiority for preparation of biodegradable intestinal anastomosis rings. Med Sci Monit 20: 1056-1066.
[50]
Amresh GR, Singh PN, Rao CV (2008) Toxicological screening of traditional medicine Laghupatha (Cissampelos pareira) in experimental animals. J Ethnopharmacol 116: 454-460.
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