Consciousness Energy Healing Treatment Based Herbomineral Formulation: A Safe and Effective Approach for Skin Health
American Journal of Pharmacology and Phytotherapy
Volume 2, Issue 1, March 2017, Pages: 1-10
Received: Mar. 29, 2017; Accepted: Apr. 26, 2017; Published: May 8, 2017
Views 1949      Downloads 78
Authors
Jagdish Singh, Trivedi Global, Inc., Henderson, Nevada, USA
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton, Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, Nevada, USA
Mayank Gangwar, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Article Tools
Follow on us
Abstract
Oxidative stress causes serious skin damage that is characterized by ageing, wrinkling, roughness, laxity and pigmentation. In the present work, the impact of Biofield Energy (The Trivedi Effect®-Consciousness Energy Healing) Treatment on the herbomineral test formulation and cell medium (DMEM) was evaluated for skin health parameters. The test formulation was consisted of minerals (zinc chloride, sodium selenate, and sodium molybdate), L-ascorbic acid, herbal (Centella asiatica) extract, and tetrahydrocurcumin (THC). The test formulation and DMEM media were divided into two parts. One part received the Biofield Energy Treatment by Jagdish Singh and was termed as the Biofield Treated (BT) sample, while other was denoted as the untreated (UT) samples. MTT assay showed that test formulation was found safe and nontoxic with greater than 75% cell viability against various tested concentrations. Cell proliferation data using BrdU method showed an improved cell proliferation by 149.13% and 118.80% at 8.75 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively compared with the untreated group. The collagen level was significantly increased by 28.14% and 44.45% at 1.25 and 0.625 µg/mL, respectively in the UT-DMEM + BT-Test formulation compared with the untreated group. The elastin level was increased by 10.38%, 14.66%, and 48.24% at 2.5, 1.25, and 0.625 µg/mL, respectively in the BT-DMEM + UT-Test formulation group, compared with the untreated group. Moreover, melanin synthesis was significantly inhibited by 5.93%, 1.43%, and 1.43% in the UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 0.0625 µg/mL compared with the untreated group. However, melanin synthesis was decreased by 11.71% and 15.75% at 0.125 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively. Anti-wrinkling effects exhibited improved cell viability by 17.19% and 28.68% at 2.5 µg/mL in the UT-DMEM + BT-Test formulation and BT-DMEM + BT-Test formulation groups, respectively in HFF-1 cells compared with the untreated group. Wound healing activity using scratch assay showed a significantly improved healing rate upto 5% in the HFF-1 and HaCaT cells lines in the Biofield Energy Healing based test formulation. Overall, the data suggests that The Trivedi Effect® treated test formulation and DMEM has the capacity to improve the skin health and suggests its use in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, and many more skin diseases.
Keywords
Consciousness Energy Healing Treatment, Extracellular Matrix, HaCaT, HFF-1, Hyaluronic Acid, Scratch Assay, Tetrahydrocurcumin
To cite this article
Jagdish Singh, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana, Consciousness Energy Healing Treatment Based Herbomineral Formulation: A Safe and Effective Approach for Skin Health, American Journal of Pharmacology and Phytotherapy. Vol. 2, No. 1, 2017, pp. 1-10. doi: 10.11648/j.ajpp.20170201.11
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Yaar M and Gilchrest BA (2007) Photoageing: mechanism, prevention and therapy. Br J Dermatol 157: 874-887.
[2]
Langton AK, Sherratt MJ, Griffiths CEM and Watson REB (2010) A new wrinkle on old skin: the role of elastic fibres in skin ageing. Int J Cosmet Sci 32: 330-339.
[3]
Warren R, Gartstein V, Kligman AM, Montagna W, Allendorf RA and Ridder GM (1991) Age, sunlight, and facial skin: a histologic and quantitative study. J Am Acad Dermatol 25: 751-760.
[4]
Tabassum N, Hamdani M (2014) Plants used to treat skin diseases. Pharmacogn Rev 8: 52-60.
[5]
Goyal RK (2005) Investigation of cellular and molecular mechanisms for anti-diabetic drugs with special reference to Unani and Ayurvedic herbal medicines. In: traditional system of medicine, Abdin, M. Z. and Y. P. Abrol (Eds.). Narosa Publishing House, New Delhi.
[6]
WHO (1993) Research Guideline for Evaluating the Safety and Efficacy of Herbal Medicines. World Health Organization, Manila, Philippines.
[7]
Gao XH, Zhang L, Wei H, Chen HD (2008) Efficacy and safety of innovative cosmeceuticals. Clin Dermatol 26: 367-74.
[8]
Fabricant DS, Farnsworth NR (2001) The value of plants used in traditional medicine for drug discovery. Environ Health Perspect 109: 69-75.
[9]
Park K (2015) Role of micronutrients in skin health and function. Biomol Ther (Seoul) 23: 207-217.
[10]
Hashim P (2011) Centella asiatica in food and beverage applications and its potential antioxidant and neuroprotective effect. Int Food Res J 18: 1215-1222.
[11]
Boyera N, Galey I, Bernard BA (1998) Effect of vitamin C and its derivatives on collagen synthesis and cross-linking by normal human fibroblasts. Int J Cosmet Sci 20: 151-158.
[12]
Gupta SC, Patchva S, Koh W, Aggarwal BB (2012) Discovery of curcumin, a component of golden spice, and its miraculous biological activities. Clin Exp Pharmacol Physiol 39: 283-299.
[13]
Majeed M, Badmaev V, Uma S, Rajenderan JR (1995) Curcuminoids: Antioxidant Phytonutrients, Nutreiscience publishers New Jersey 1-24.
[14]
Sugiyama Y, Kawakishi S, Osawa T (1996) Involvement of the β-diketone moiety in the antioxidant mechanism of tetrahydrocurcuminoids. Biochem Pharmacol 52: 519-525.
[15]
Bylka W, Znajdek-Awiżeń P, Studzińska-Sroka E, Brzezińska M (2013) Centella asiatica in cosmetology. Postepy Dermatol Alergol 30: 46-49.
[16]
Rubik B, Muehsam D, Hammerschlag R, Jain S (2015) Biofield science and healing: history, terminology, and concepts. Glob Adv Health Med 4: 8-14.
[17]
Barnes PM, Bloom B, Nahin RL (2008) Complementary and alternative medicine use among adults and children: United States, 2007. Natl Health Stat Report 12: 1-23.
[18]
Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, Mondal SC, Jana S (2015) Antibiogram pattern of Shigella flexneri: Effect of biofield treatment. Air Water Borne Diseases 3: 122.
[19]
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) Antimicrobial susceptibility pattern and biochemical characteristics of Staphylococcus aureus: Impact of biofield treatment. J Microb Biochem Technol 7: 238-241.
[20]
Trivedi MK, Branton A, Trivedi D, Nayak G, Shettigar H, Mondal SC, Jana S (2015) Effect of biofield energy treatment on Streptococcus group B: A postpartum pathogen. J Microb Biochem Technol 7: 269-273.
[21]
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Morphological and molecular analysis using RAPD in biofield treated sponge and bitter gourd. American Journal of Agriculture and Forestry 3: 264-270.
[22]
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Effect of biofield energy treatment on chlorophyll content, pathological study, and molecular analysis of cashew plant (Anacardium occidentale L.). Journal of Plant Sciences 3: 372-382.
[23]
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2016) Molecular analysis of biofield treated eggplant and watermelon crops. Adv Crop Sci Tech 4: 208.
[24]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Effect of biofield treated energized water on the growth and health status in chicken (Gallus gallus domesticus). Poult Fish Wildl Sci 3: 140.
[25]
Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) An evaluation of biofield treatment on thermal, physical and structural properties of cadmium powder. J Thermodyn Catal 6: 147.
[26]
Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) Effect of Biofield energy treatment on physical and structural properties of calcium carbide and praseodymium oxide. International Journal of Materials Science and Applications 4: 390-395.
[27]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Characterization of physical, thermal and structural properties of chromium (VI) oxide powder: Impact of biofield treatment. J Powder Metall Min 4: 128.
[28]
Plumb JA (2004) Cell sensitivity assays: the MTT assay. Methods Mol Med 88: 165-169.
[29]
Hahn MS, Kobler JB, Starcher BC, Zeitels SM, Langer R (2006) Quantitative and comparative studies of the vocal fold extracellular matrix. I: Elastic fibers and hyaluronic acid. Ann Otol Rhinol Laryngol 115: 156-164.
[30]
Zhang L, Yoshida T, Kuroiwa Y (1992) Stimulation of melanin synthesis of B16-F10 mouse melanoma cells by bufalin. Life Sci 51: 17-24.
[31]
Shoulders MD, Raines RT (2009) Collagen structure and stability. Annual review of biochemistry 78: 929-958.
[32]
Fronza M, Heinzmann B, Hamburger M, Laufer S, Merfort I (2009) Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. J Ethnopharmacol 126: 463-467.
[33]
Kadler KE, Holmes DF, Trotter JA, Chapman JA (1996) Collagen fibril formation. Biochemical Journal 316: 1-11.
[34]
Shoulders MD, Raines RT (2009) Collagen structure and stability. Annual review of biochemistry 78: 929-958.
[35]
Frantz C, Stewart KM, Weaver VM (2010) The extracellular matrix at a glance. J Cell Sci 123: 4195-4200.
[36]
Weindl G, Schaller M, Schäfer-Korting M, Korting HC (2004) Hyaluronic acid in the treatment and prevention of skin diseases: molecular biological, pharmaceutical and clinical aspects. Skin Pharmacol Physiol 17: 207-213.
[37]
Busca R, Ballotti R (2000) Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigment Cell Res 13: 60-69.
[38]
Bylka W, Znajdek-Awiżeń P, Studzińska-Sroka E, Brzezińska M (2013) Centella asiatica in cosmetology. Postepy Dermatol Alergol 30: 46-49.
[39]
Prasad S, Tyagi AK, Aggarwal BB (2014) Recent developments in delivery, bioavailability, absorption and metabolism of curcumin: the golden pigment from golden spice. Cancer Res Treat 46: 2-18.
[40]
Alaluf S, Atkins D, Barrett K, Blount M, Carter N, Heath A. (2002) The impact of epidermal melanin on objective measurements of human skin colour. Pigment Cell Res. 15: 119-126.
[41]
Ho JN, Lee YH, Lee YD, Jun WJ, Kim HK, Hong BS, Shin DH, Cho HY (2005) Inhibitory effect of Aucubin isolated from Eucommia ulmoides against UVB induced matrix metalloproteinase-1 production in human skin fibroblasts. Biosci Biotechnol Biochem 69: 2227-2231.
[42]
Liang CC, Park AY, Guan JL (2007) In vitro scratch assay: A convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2: 329-333.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186