Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of <i>Helicobacter Pylori</i> Isolates from South of Russia

Vladimir Mihajlovich Sorokin; Ruslan Vjacheslavovich Pisanov; Elena Vadimovna Golubkina; Elena Aleksandrovna Bereznyak; Lubov Aleksandrovna Prozorova

doi:doi:10.11648/j.ijmb.20170203.15

Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia

Vladimir Mihajlovich Sorokin, Ruslan Vjacheslavovich Pisanov, Elena Vadimovna Golubkina, Elena Aleksandrovna Bereznyak, Lubov Aleksandrovna Prozorova

Published in International Journal of Microbiology and Biotechnology (Volume 2, Issue 3)

Received: 3 October 2016 Accepted: 19 March 2017 Published: 18 April 2017

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Abstract

High genetic diversity and rapid microevolution are a peculiarity of genomes Helicobacter pylori, a phenomenon that is proposed to play a functional role in persistence and colonisation of diverse human populations. Isolates of H. pylori can be classified phylogeographically. To characterise diverse strains of this pathogen in different human populations, we compare MLVA genotypes of 48 H. pylori strains isolated from different regions in the South of Russia. Cluster analysis identified 48 individual MLVA types (MTs) and revealed the possible belonging the greatest part of the strains to hpEurope, and two strains to hpEastAsia population, when compared to the 4 publically available genomes. The proposed method may be successfully used in molecular epidemiology and possibly for population identification of H. pylori. A similar study was conducted in Russia and Europe for the first time.

Published in	International Journal of Microbiology and Biotechnology (Volume 2, Issue 3)
DOI	10.11648/j.ijmb.20170203.15
Page(s)	135-138
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Helicobacter Pylori, VNTR Genotyping, MLVA, Cluster Analysis, Molecular Epidemiology

References

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APA Style

Vladimir Mihajlovich Sorokin, Ruslan Vjacheslavovich Pisanov, Elena Vadimovna Golubkina, Elena Aleksandrovna Bereznyak, Lubov Aleksandrovna Prozorova. (2017). Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia. International Journal of Microbiology and Biotechnology, 2(3), 135-138. https://doi.org/10.11648/j.ijmb.20170203.15

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ACS Style

Vladimir Mihajlovich Sorokin; Ruslan Vjacheslavovich Pisanov; Elena Vadimovna Golubkina; Elena Aleksandrovna Bereznyak; Lubov Aleksandrovna Prozorova. Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia. Int. J. Microbiol. Biotechnol. 2017, 2(3), 135-138. doi: 10.11648/j.ijmb.20170203.15

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AMA Style

Vladimir Mihajlovich Sorokin, Ruslan Vjacheslavovich Pisanov, Elena Vadimovna Golubkina, Elena Aleksandrovna Bereznyak, Lubov Aleksandrovna Prozorova. Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia. Int J Microbiol Biotechnol. 2017;2(3):135-138. doi: 10.11648/j.ijmb.20170203.15

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@article{10.11648/j.ijmb.20170203.15,
  author = {Vladimir Mihajlovich Sorokin and Ruslan Vjacheslavovich Pisanov and Elena Vadimovna Golubkina and Elena Aleksandrovna Bereznyak and Lubov Aleksandrovna Prozorova},
  title = {Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {2},
  number = {3},
  pages = {135-138},
  doi = {10.11648/j.ijmb.20170203.15},
  url = {https://doi.org/10.11648/j.ijmb.20170203.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20170203.15},
  abstract = {High genetic diversity and rapid microevolution are a peculiarity of genomes Helicobacter pylori, a phenomenon that is proposed to play a functional role in persistence and colonisation of diverse human populations. Isolates of H. pylori can be classified phylogeographically. To characterise diverse strains of this pathogen in different human populations, we compare MLVA genotypes of 48 H. pylori strains isolated from different regions in the South of Russia. Cluster analysis identified 48 individual MLVA types (MTs) and revealed the possible belonging the greatest part of the strains to hpEurope, and two strains to hpEastAsia population, when compared to the 4 publically available genomes. The proposed method may be successfully used in molecular epidemiology and possibly for population identification of H. pylori. A similar study was conducted in Russia and Europe for the first time.},
 year = {2017}
}

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TY - JOUR
T1 - Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia
AU - Vladimir Mihajlovich Sorokin
AU - Ruslan Vjacheslavovich Pisanov
AU - Elena Vadimovna Golubkina
AU - Elena Aleksandrovna Bereznyak
AU - Lubov Aleksandrovna Prozorova
Y1 - 2017/04/18
PY - 2017
N1 - https://doi.org/10.11648/j.ijmb.20170203.15
DO - 10.11648/j.ijmb.20170203.15
T2 - International Journal of Microbiology and Biotechnology
JF - International Journal of Microbiology and Biotechnology
JO - International Journal of Microbiology and Biotechnology
SP - 135
EP - 138
PB - Science Publishing Group
SN - 2578-9686
UR - https://doi.org/10.11648/j.ijmb.20170203.15
AB - High genetic diversity and rapid microevolution are a peculiarity of genomes Helicobacter pylori, a phenomenon that is proposed to play a functional role in persistence and colonisation of diverse human populations. Isolates of H. pylori can be classified phylogeographically. To characterise diverse strains of this pathogen in different human populations, we compare MLVA genotypes of 48 H. pylori strains isolated from different regions in the South of Russia. Cluster analysis identified 48 individual MLVA types (MTs) and revealed the possible belonging the greatest part of the strains to hpEurope, and two strains to hpEastAsia population, when compared to the 4 publically available genomes. The proposed method may be successfully used in molecular epidemiology and possibly for population identification of H. pylori. A similar study was conducted in Russia and Europe for the first time.
VL - 2
IS - 3
ER -

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Author Information

Vladimir Mihajlovich Sorokin

Department of Biochemistry, Rostov-on-Don Research Institute for Plague Control, Rostov-on-Don, Russia
Ruslan Vjacheslavovich Pisanov

Department of Biochemistry, Rostov-on-Don Research Institute for Plague Control, Rostov-on-Don, Russia
Elena Vadimovna Golubkina

Department of Biochemistry, Astrakhan State Medical University, Ministry of Health of Russia, Astrahan', Russia
Elena Aleksandrovna Bereznyak

Department of Biochemistry, Rostov-on-Don Research Institute for Plague Control, Rostov-on-Don, Russia
Lubov Aleksandrovna Prozorova

Department of Biochemistry, Rostov-on-Don Research Institute for Plague Control, Rostov-on-Don, Russia

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APA Style

Vladimir Mihajlovich Sorokin, Ruslan Vjacheslavovich Pisanov, Elena Vadimovna Golubkina, Elena Aleksandrovna Bereznyak, Lubov Aleksandrovna Prozorova. (2017). Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia. International Journal of Microbiology and Biotechnology, 2(3), 135-138. https://doi.org/10.11648/j.ijmb.20170203.15

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ACS Style

Vladimir Mihajlovich Sorokin; Ruslan Vjacheslavovich Pisanov; Elena Vadimovna Golubkina; Elena Aleksandrovna Bereznyak; Lubov Aleksandrovna Prozorova. Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia. Int. J. Microbiol. Biotechnol. 2017, 2(3), 135-138. doi: 10.11648/j.ijmb.20170203.15

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AMA Style

Vladimir Mihajlovich Sorokin, Ruslan Vjacheslavovich Pisanov, Elena Vadimovna Golubkina, Elena Aleksandrovna Bereznyak, Lubov Aleksandrovna Prozorova. Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia. Int J Microbiol Biotechnol. 2017;2(3):135-138. doi: 10.11648/j.ijmb.20170203.15

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@article{10.11648/j.ijmb.20170203.15,
  author = {Vladimir Mihajlovich Sorokin and Ruslan Vjacheslavovich Pisanov and Elena Vadimovna Golubkina and Elena Aleksandrovna Bereznyak and Lubov Aleksandrovna Prozorova},
  title = {Comparative Multiple-Locus Variable-Number Tandem Repeat Analysis of Helicobacter Pylori Isolates from South of Russia},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {2},
  number = {3},
  pages = {135-138},
  doi = {10.11648/j.ijmb.20170203.15},
  url = {https://doi.org/10.11648/j.ijmb.20170203.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20170203.15},
  abstract = {High genetic diversity and rapid microevolution are a peculiarity of genomes Helicobacter pylori, a phenomenon that is proposed to play a functional role in persistence and colonisation of diverse human populations. Isolates of H. pylori can be classified phylogeographically. To characterise diverse strains of this pathogen in different human populations, we compare MLVA genotypes of 48 H. pylori strains isolated from different regions in the South of Russia. Cluster analysis identified 48 individual MLVA types (MTs) and revealed the possible belonging the greatest part of the strains to hpEurope, and two strains to hpEastAsia population, when compared to the 4 publically available genomes. The proposed method may be successfully used in molecular epidemiology and possibly for population identification of H. pylori. A similar study was conducted in Russia and Europe for the first time.},
 year = {2017}
}

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