International Journal of Microbiology and Biotechnology

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Clinical Assessment of Lactobacillus gasseri Strain, Selected by In-Vitro Tests

Received: 27 April 2017    Accepted: 22 May 2017    Published: 16 June 2017
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Abstract

Lactobacillus gasseri G4 strain isolated from healthy human volunteer was selected as one of the best adhesive strain among a large group of lactobacilli. In-vitro tests for establishment of immunomodulatory potential of selected lactic acid bacteria were carried out. Three different analytical models were used for evaluation, according to the specifics of each different cytokine and to compare, where possible. The induction of cytokines like: interleukin-1 (IL-1), IL-10 and tumor necrosis factor alpha (TNF-α) were determined by widely used laboratory approach with mouse splenocytes as well as on combined (well-in-well) monocytic and epithelium human cell lines (THP-1 and HT-29). Other cytokines like IL-8 and IL-4 were evaluated by only one analytical model, while transforming growth factor beta (TGF-β) was evaluated by using all of the three models. The selected strain shows significant increase of IL-10, interferon gamma (IFN-γ) and TGF-β production in-vitro. Therefore, this strain was directed to clinical trials with 38 healthy volunteers, randomized and placebo-controlled. The results demonstrate statistically significant increase of TGF-β production (25%). Immunoglobulin A (IgA) and IFN-γ were also increased. Stable levels of IL-10 were observed after the in-vivo experiments with L. gasseri G4 strain. The selected strain does not increase in-vivo the pro-inflammatory cytokine IL-8. In this study the immunomodulatory properties of L. gasseri G4 were confirmed in-vivo therefore it is suitable for development of probiotic formulas.

DOI 10.11648/j.ijmb.20170204.11
Published in International Journal of Microbiology and Biotechnology (Volume 2, Issue 4, November 2017)
Page(s) 156-160
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Probiotics, Lactobacillus gasseri, Immunomodulation, Cytokines, Immunoglobulin

References
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[4] Cummings JH Antoine JM, Azpiroz F, Bourdet-Sicard R, Brandtzaeg P, et al. (2004) PASSCLAIM--gut health and immunity.Eur J Nutr 43 Suppl 2: II118-118II173.
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[7] Gill H. S.,Rutherfurd K. J., Cross M. L. & Gopal P. K. (2001). Enhancement of immunity in the elderly by dietary supplementation with the probiotic Bifidobacterium lactis HN019. Am J Clin Nutrit 74:833-839.
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[18] Clements, L. M., R. F. Betts, E. L. Tierney, and B. R. Murphy. 1986. Serum and nasal wash antibodies associated with resistance to experimental challenge with influenza A wild-type virus. J. Clin. Microbiol. 24:157–160.
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Author Information
  • R&D Center LB Bulgaricum Plc, Sofia, Bulgaria

  • R&D Center LB Bulgaricum Plc, Sofia, Bulgaria

  • The Stefan Angelov Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria

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    Irina Marinova Gotova, Zhechko Panayotov Dimitrov, Hristo Miladinov Naidenski. (2017). Clinical Assessment of Lactobacillus gasseri Strain, Selected by In-Vitro Tests. International Journal of Microbiology and Biotechnology, 2(4), 156-160. https://doi.org/10.11648/j.ijmb.20170204.11

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    Irina Marinova Gotova; Zhechko Panayotov Dimitrov; Hristo Miladinov Naidenski. Clinical Assessment of Lactobacillus gasseri Strain, Selected by In-Vitro Tests. Int. J. Microbiol. Biotechnol. 2017, 2(4), 156-160. doi: 10.11648/j.ijmb.20170204.11

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    AMA Style

    Irina Marinova Gotova, Zhechko Panayotov Dimitrov, Hristo Miladinov Naidenski. Clinical Assessment of Lactobacillus gasseri Strain, Selected by In-Vitro Tests. Int J Microbiol Biotechnol. 2017;2(4):156-160. doi: 10.11648/j.ijmb.20170204.11

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  • @article{10.11648/j.ijmb.20170204.11,
      author = {Irina Marinova Gotova and Zhechko Panayotov Dimitrov and Hristo Miladinov Naidenski},
      title = {Clinical Assessment of Lactobacillus gasseri Strain, Selected by In-Vitro Tests},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {2},
      number = {4},
      pages = {156-160},
      doi = {10.11648/j.ijmb.20170204.11},
      url = {https://doi.org/10.11648/j.ijmb.20170204.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijmb.20170204.11},
      abstract = {Lactobacillus gasseri G4 strain isolated from healthy human volunteer was selected as one of the best adhesive strain among a large group of lactobacilli. In-vitro tests for establishment of immunomodulatory potential of selected lactic acid bacteria were carried out. Three different analytical models were used for evaluation, according to the specifics of each different cytokine and to compare, where possible. The induction of cytokines like: interleukin-1 (IL-1), IL-10 and tumor necrosis factor alpha (TNF-α) were determined by widely used laboratory approach with mouse splenocytes as well as on combined (well-in-well) monocytic and epithelium human cell lines (THP-1 and HT-29). Other cytokines like IL-8 and IL-4 were evaluated by only one analytical model, while transforming growth factor beta (TGF-β) was evaluated by using all of the three models. The selected strain shows significant increase of IL-10, interferon gamma (IFN-γ) and TGF-β production in-vitro. Therefore, this strain was directed to clinical trials with 38 healthy volunteers, randomized and placebo-controlled. The results demonstrate statistically significant increase of TGF-β production (25%). Immunoglobulin A (IgA) and IFN-γ were also increased. Stable levels of IL-10 were observed after the in-vivo experiments with L. gasseri G4 strain. The selected strain does not increase in-vivo the pro-inflammatory cytokine IL-8. In this study the immunomodulatory properties of L. gasseri G4 were confirmed in-vivo therefore it is suitable for development of probiotic formulas.},
     year = {2017}
    }
    

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    T1  - Clinical Assessment of Lactobacillus gasseri Strain, Selected by In-Vitro Tests
    AU  - Irina Marinova Gotova
    AU  - Zhechko Panayotov Dimitrov
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    JO  - International Journal of Microbiology and Biotechnology
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    AB  - Lactobacillus gasseri G4 strain isolated from healthy human volunteer was selected as one of the best adhesive strain among a large group of lactobacilli. In-vitro tests for establishment of immunomodulatory potential of selected lactic acid bacteria were carried out. Three different analytical models were used for evaluation, according to the specifics of each different cytokine and to compare, where possible. The induction of cytokines like: interleukin-1 (IL-1), IL-10 and tumor necrosis factor alpha (TNF-α) were determined by widely used laboratory approach with mouse splenocytes as well as on combined (well-in-well) monocytic and epithelium human cell lines (THP-1 and HT-29). Other cytokines like IL-8 and IL-4 were evaluated by only one analytical model, while transforming growth factor beta (TGF-β) was evaluated by using all of the three models. The selected strain shows significant increase of IL-10, interferon gamma (IFN-γ) and TGF-β production in-vitro. Therefore, this strain was directed to clinical trials with 38 healthy volunteers, randomized and placebo-controlled. The results demonstrate statistically significant increase of TGF-β production (25%). Immunoglobulin A (IgA) and IFN-γ were also increased. Stable levels of IL-10 were observed after the in-vivo experiments with L. gasseri G4 strain. The selected strain does not increase in-vivo the pro-inflammatory cytokine IL-8. In this study the immunomodulatory properties of L. gasseri G4 were confirmed in-vivo therefore it is suitable for development of probiotic formulas.
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