Isolation and Identification of Airborne Pathogenic Fungi from the Hospitals at Dhamar Governorate, Yemen

Hameed Ahmed Golah; Maged Ahmed Al-Garadi; Mohamed Salah; Najla Baghza; Hesham Al-Mahdi; Mohamed Al-Dhorani; Abdulgani Al-Sharma

doi:doi:10.11648/j.ijmb.20170204.13

Isolation and Identification of Airborne Pathogenic Fungi from the Hospitals at Dhamar Governorate, Yemen

Hameed Ahmed Golah, Maged Ahmed Al-Garadi, Mohamed Salah, Najla Baghza, Hesham Al-Mahdi, Mohamed Al-Dhorani, Abdulgani Al-Sharma

Published in International Journal of Microbiology and Biotechnology (Volume 2, Issue 4)

Received: 24 March 2017 Accepted: 13 June 2017 Published: 14 July 2017

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

The air in many indoor environments also contains spores; however, the hospital indoors environments may lead to spread the pathogenic fungi spores among hospitalized patients. The aim of this study was to isolate and identify the airborne pathogenic fungi from the Hospitals environment at Dhamar governorate, Yemen. The study was conducted in four hospitals of government, which was included; Hospital A, Hospital B, Hospital C and Hospital D. A total of 48 air samples was collected from different departments of these Hospitals. By using sterile petri dishes contains sabouraud dextrose agar (SDA) media with 50µg/L of Cychlohexamide as anti- microbial, to prevent growth of saprophytic fungi and some bacteria. These petri dishes were left open for 6 hours. All samples labeled properly and brought to a laboratory for examination and processing according to standard microbiological techniques. The results had revealed that, 34 pathogenic and opportunistic fungi were isolated from Four Hospitals at Dhamar governorate. These isolates were distributed in Hospital A 35% (12/34), Hospital 21% (7/34) B, Hospital 18% (6/34) C and Hospital D 26% (9/34). These airborne pathogenic fungi included 8 fungal genera: Trichophyton, Cladosporium, Chryosporium, Mortierella, Paecilomyces, Aspergillus Rhizopus and Penicillinum spp.. Overall the result, only 6 (18%) isolates were identified as pathogenic fungi at all Dhamar Hospital while the rest 28 isolates were identified as opportunistic fungi at all Dhamar governorates hospitals. In conclusion, more hygienic practices and continuous checking of nosocomial pathogen should be taken under consideration.

Published in	International Journal of Microbiology and Biotechnology (Volume 2, Issue 4)
DOI	10.11648/j.ijmb.20170204.13
Page(s)	166-170
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Airborne Fungi, Dhamar, Hospitals, Isolation, Identification and Yemen

References

[1]	BARRON, G. L. 1968. The genera of Hyphomycetes from soil, New York, Robert E. Krieger Publishing Company Huntington.
[2]	BEGGS, C. 2003. The airborne transmission of infection in hospital buildings: fact or fiction? Indoor and Built Environment, 12, 9-18.
[3]	CAUWENBERGH, G. 1985. New and prospective developments in antifungal drugs. Acta dermato-venereologica. Supplementum, 121, 147-153.
[4]	CHAYA, A. & PANDE, S. 2007. Methods of specimen collection for diagnosis of superficial and subcutaneous fungal infections. Indian Journal of Dermatology, Venereology, and Leprology, 73, 202.
[5]	DANIEL, W. W. 1987. Biostatistics A Foundation for Analysis:. New York.
[6]	FATHI, H. & AL-SAMARAI, A. 1997. Tinea capitis in Iraq: labratory results.
[7]	Eastern Mediterranean. Health J, 6, 138-148.
[8]	JAFFAL, A., NSANZE, H., BENER, A., AMEEN, A., BANAT, I. & EL MOGHETH, A. 1997. Hospital airborne microbial pollution in a desert country. Environment international, 23, 167-172.
[9]	KONEMAN, W., ROBERTS, G. D. & WRIGHT, S. E. 1978. Paractical laboratory mycology, U.S, Williams and Wilkins Company, Baltimor.
[10]	KWON-CHUNG, K. J. & BENNETT, J. E. 1992. Medical mycology. Revista do Instituto de Medicina Tropical de São Paulo, 34, 504-504.
[11]	MIRANDA, M. F. & SILVA, A. J. 2005. Vinyl adhesive tape also effective for direct microscopy diagnosis of chromomycosis, lobomycosis, and paracoccidioidomycosis. Diagnostic microbiology and infectious disease, 52, 39-43.
[12]	PRESCOTT, L., HARLEY, J. & KLEIN, D. 1993. Microbiology', Wm C Brown Communications. Inc., 2nd. Edition, USA, 912.
[13]	SAAD, S. G. 2003. Integrated environmental management for hospitals. Indoor and Built Environment, 12, 93-98.
[14]	SINGH, S. & BEENA, P. 2003. Comparative study of different microscopic techniques and culture media for the isolation of dermatophytes. Indian journal of medical microbiology, 21, 21.
[15]	TORTORA, G. J., FUNKE, B. R., CHRISTINE & CASE, L. 2007. Microbiology: An Introduction. Benjamin Cummings.
[16]	WEITZMAN, I. & SUMMERBELL, R. C. 1995. The dermatophytes. Clinical microbiology reviews, 8, 240-259.
[17]	WHO 2002. The world health report 2002: reducing risks, promoting healthy life, World Health Organization.
[18]	ZOMORODIAN, K., EMAMI, M., TARAZOEI, B. & SAADAT, F. 2002. Study and identification of the etiological agents of onychomycosis in Tehran, capital of Iran. Iranian Journal of Public Health, 31, 100-104.

Cite This Article

Plain Text BibTeX RIS

APA Style

Hameed Ahmed Golah, Maged Ahmed Al-Garadi, Mohamed Salah, Najla Baghza, Hesham Al-Mahdi, et al. (2017). Isolation and Identification of Airborne Pathogenic Fungi from the Hospitals at Dhamar Governorate, Yemen. International Journal of Microbiology and Biotechnology, 2(4), 166-170. https://doi.org/10.11648/j.ijmb.20170204.13

Copy | Download

ACS Style

Hameed Ahmed Golah; Maged Ahmed Al-Garadi; Mohamed Salah; Najla Baghza; Hesham Al-Mahdi, et al. Isolation and Identification of Airborne Pathogenic Fungi from the Hospitals at Dhamar Governorate, Yemen. Int. J. Microbiol. Biotechnol. 2017, 2(4), 166-170. doi: 10.11648/j.ijmb.20170204.13

Copy | Download

AMA Style

Hameed Ahmed Golah, Maged Ahmed Al-Garadi, Mohamed Salah, Najla Baghza, Hesham Al-Mahdi, et al. Isolation and Identification of Airborne Pathogenic Fungi from the Hospitals at Dhamar Governorate, Yemen. Int J Microbiol Biotechnol. 2017;2(4):166-170. doi: 10.11648/j.ijmb.20170204.13

Copy | Download

@article{10.11648/j.ijmb.20170204.13,
  author = {Hameed Ahmed Golah and Maged Ahmed Al-Garadi and Mohamed Salah and Najla Baghza and Hesham Al-Mahdi and Mohamed Al-Dhorani and Abdulgani Al-Sharma},
  title = {Isolation and Identification of Airborne Pathogenic Fungi from the Hospitals at Dhamar Governorate, Yemen},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {2},
  number = {4},
  pages = {166-170},
  doi = {10.11648/j.ijmb.20170204.13},
  url = {https://doi.org/10.11648/j.ijmb.20170204.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20170204.13},
  abstract = {The air in many indoor environments also contains spores; however, the hospital indoors environments may lead to spread the pathogenic fungi spores among hospitalized patients. The aim of this study was to isolate and identify the airborne pathogenic fungi from the Hospitals environment at Dhamar governorate, Yemen. The study was conducted in four hospitals of government, which was included; Hospital A, Hospital B, Hospital C and Hospital D. A total of 48 air samples was collected from different departments of these Hospitals. By using sterile petri dishes contains sabouraud dextrose agar (SDA) media with 50µg/L of Cychlohexamide as anti- microbial, to prevent growth of saprophytic fungi and some bacteria. These petri dishes were left open for 6 hours. All samples labeled properly and brought to a laboratory for examination and processing according to standard microbiological techniques. The results had revealed that, 34 pathogenic and opportunistic fungi were isolated from Four Hospitals at Dhamar governorate. These isolates were distributed in Hospital A 35% (12/34), Hospital 21% (7/34) B, Hospital 18% (6/34) C and Hospital D 26% (9/34). These airborne pathogenic fungi included 8 fungal genera: Trichophyton, Cladosporium, Chryosporium, Mortierella, Paecilomyces, Aspergillus Rhizopus and Penicillinum spp.. Overall the result, only 6 (18%) isolates were identified as pathogenic fungi at all Dhamar Hospital while the rest 28 isolates were identified as opportunistic fungi at all Dhamar governorates hospitals. In conclusion, more hygienic practices and continuous checking of nosocomial pathogen should be taken under consideration.},
 year = {2017}
}

Copy | Download

TY - JOUR
T1 - Isolation and Identification of Airborne Pathogenic Fungi from the Hospitals at Dhamar Governorate, Yemen
AU - Hameed Ahmed Golah
AU - Maged Ahmed Al-Garadi
AU - Mohamed Salah
AU - Najla Baghza
AU - Hesham Al-Mahdi
AU - Mohamed Al-Dhorani
AU - Abdulgani Al-Sharma
Y1 - 2017/07/14
PY - 2017
N1 - https://doi.org/10.11648/j.ijmb.20170204.13
DO - 10.11648/j.ijmb.20170204.13
T2 - International Journal of Microbiology and Biotechnology
JF - International Journal of Microbiology and Biotechnology
JO - International Journal of Microbiology and Biotechnology
SP - 166
EP - 170
PB - Science Publishing Group
SN - 2578-9686
UR - https://doi.org/10.11648/j.ijmb.20170204.13
AB - The air in many indoor environments also contains spores; however, the hospital indoors environments may lead to spread the pathogenic fungi spores among hospitalized patients. The aim of this study was to isolate and identify the airborne pathogenic fungi from the Hospitals environment at Dhamar governorate, Yemen. The study was conducted in four hospitals of government, which was included; Hospital A, Hospital B, Hospital C and Hospital D. A total of 48 air samples was collected from different departments of these Hospitals. By using sterile petri dishes contains sabouraud dextrose agar (SDA) media with 50µg/L of Cychlohexamide as anti- microbial, to prevent growth of saprophytic fungi and some bacteria. These petri dishes were left open for 6 hours. All samples labeled properly and brought to a laboratory for examination and processing according to standard microbiological techniques. The results had revealed that, 34 pathogenic and opportunistic fungi were isolated from Four Hospitals at Dhamar governorate. These isolates were distributed in Hospital A 35% (12/34), Hospital 21% (7/34) B, Hospital 18% (6/34) C and Hospital D 26% (9/34). These airborne pathogenic fungi included 8 fungal genera: Trichophyton, Cladosporium, Chryosporium, Mortierella, Paecilomyces, Aspergillus Rhizopus and Penicillinum spp.. Overall the result, only 6 (18%) isolates were identified as pathogenic fungi at all Dhamar Hospital while the rest 28 isolates were identified as opportunistic fungi at all Dhamar governorates hospitals. In conclusion, more hygienic practices and continuous checking of nosocomial pathogen should be taken under consideration.
VL - 2
IS - 4
ER -

Copy | Download

Author Information

Hameed Ahmed Golah

Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Thamar University, Dhamar, Yemen
Maged Ahmed Al-Garadi

Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Thamar University, Dhamar, Yemen
Mohamed Salah

Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Thamar University, Dhamar, Yemen
Najla Baghza

Department of Microbiology, Faculty of Medicine and Human Health Science, Thamar University, Dhamar, Yemen
Hesham Al-Mahdi

Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Thamar University, Dhamar, Yemen
Mohamed Al-Dhorani

Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Thamar University, Dhamar, Yemen
Abdulgani Al-Sharma

Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Thamar University, Dhamar, Yemen

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Hameed Ahmed Golah, Maged Ahmed Al-Garadi, Mohamed Salah, Najla Baghza, Hesham Al-Mahdi, et al. (2017). Isolation and Identification of Airborne Pathogenic Fungi from the Hospitals at Dhamar Governorate, Yemen. International Journal of Microbiology and Biotechnology, 2(4), 166-170. https://doi.org/10.11648/j.ijmb.20170204.13

Copy | Download

ACS Style

Hameed Ahmed Golah; Maged Ahmed Al-Garadi; Mohamed Salah; Najla Baghza; Hesham Al-Mahdi, et al. Isolation and Identification of Airborne Pathogenic Fungi from the Hospitals at Dhamar Governorate, Yemen. Int. J. Microbiol. Biotechnol. 2017, 2(4), 166-170. doi: 10.11648/j.ijmb.20170204.13

Copy | Download

AMA Style

Hameed Ahmed Golah, Maged Ahmed Al-Garadi, Mohamed Salah, Najla Baghza, Hesham Al-Mahdi, et al. Isolation and Identification of Airborne Pathogenic Fungi from the Hospitals at Dhamar Governorate, Yemen. Int J Microbiol Biotechnol. 2017;2(4):166-170. doi: 10.11648/j.ijmb.20170204.13

Copy | Download

@article{10.11648/j.ijmb.20170204.13,
  author = {Hameed Ahmed Golah and Maged Ahmed Al-Garadi and Mohamed Salah and Najla Baghza and Hesham Al-Mahdi and Mohamed Al-Dhorani and Abdulgani Al-Sharma},
  title = {Isolation and Identification of Airborne Pathogenic Fungi from the Hospitals at Dhamar Governorate, Yemen},
  journal = {International Journal of Microbiology and Biotechnology},
  volume = {2},
  number = {4},
  pages = {166-170},
  doi = {10.11648/j.ijmb.20170204.13},
  url = {https://doi.org/10.11648/j.ijmb.20170204.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20170204.13},
  abstract = {The air in many indoor environments also contains spores; however, the hospital indoors environments may lead to spread the pathogenic fungi spores among hospitalized patients. The aim of this study was to isolate and identify the airborne pathogenic fungi from the Hospitals environment at Dhamar governorate, Yemen. The study was conducted in four hospitals of government, which was included; Hospital A, Hospital B, Hospital C and Hospital D. A total of 48 air samples was collected from different departments of these Hospitals. By using sterile petri dishes contains sabouraud dextrose agar (SDA) media with 50µg/L of Cychlohexamide as anti- microbial, to prevent growth of saprophytic fungi and some bacteria. These petri dishes were left open for 6 hours. All samples labeled properly and brought to a laboratory for examination and processing according to standard microbiological techniques. The results had revealed that, 34 pathogenic and opportunistic fungi were isolated from Four Hospitals at Dhamar governorate. These isolates were distributed in Hospital A 35% (12/34), Hospital 21% (7/34) B, Hospital 18% (6/34) C and Hospital D 26% (9/34). These airborne pathogenic fungi included 8 fungal genera: Trichophyton, Cladosporium, Chryosporium, Mortierella, Paecilomyces, Aspergillus Rhizopus and Penicillinum spp.. Overall the result, only 6 (18%) isolates were identified as pathogenic fungi at all Dhamar Hospital while the rest 28 isolates were identified as opportunistic fungi at all Dhamar governorates hospitals. In conclusion, more hygienic practices and continuous checking of nosocomial pathogen should be taken under consideration.},
 year = {2017}
}

Copy | Download