Background: Respiratory viruses cause a variety of human infections, ranging from the common cold to life-threatening pneumonia. Over 200 strains of virus can cause respiratory disease. The majority of severe viral respiratory infections are caused by relatively few viruses, primarily parainfluenza virus types 1, 2 and 3, respiratory syncytial virus (RSV), influenza A and B viruses, and adenovirus. Objective: The purpose of this study was to see the validity of Immunofluorescence test for the detection of Respiratory viruses causing acute lower respiratory tract infection among under five children. Methodology: This cross sectional study was conducted in the Department of Virology at Bangabandhu Sheikh Mujib Medical University, Dhaka from July 2002 to June 2003 for a period of one year. The children with the age group of below five (5) years presented with the clinical manifestations of acute lower respiratory tract infection (ALRI) who were visited or were admitted to Dhaka Medical College Hospital (DMCH), Dhaka were selected as study population. Nasopharyngeal aspirates were collected. Viruses were detected by cell line culture and direct Immunoflorescence (DFA) method. Result: The study was carried out among 100 children aged from new born to 60 months with acute lower respiratory tract infection (ALRI). Highest rate (85.7%) of isolation was obtained among children between 0 to 24 months of age. There was a significant reduction in the number of cases in older children in 25 to 60 months of age group. The most common virus isolated from the under five children was respiratory syncytial virus which was 20(95.2%). Adenovirus was isolated in only 1(4.8%) case. No other viruses were found in this study. DFA method typically more rapid than the cell culture and also detect virus which has lost viability in transit. Culture methods on the other hand, are more favorable for detecting low titer of viable virus. In this study 17 samples are positive by cell culture and these are also positive by DFA. Total 21 samples are positive by DFA and among them 4 samples are negative. Conclusion: DFA is highly sensitive and specific for detection of respiratory viruses among the under-five children. Furthermore the accuracy of this test is also very high. Therefore it is recommended that the DFA test can be used for the detection of respiratory virus from the children presented with respiratory tract infection.
| Published in | International Journal of Microbiology and Biotechnology (Volume 3, Issue 1) |
| DOI | 10.11648/j.ijmb.20180301.12 |
| Page(s) | 7-10 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Respiratory Viruses, Acute Lower Respiratory Tract Infection, Under Five Children, Cell Culture, Immunoflourescence, Test Validity
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APA Style
Rashida Akter Khanam, Md. Nazrul Islam, Shahina Tabassum, Ahmed Sharif, Md. Abdullah Yusuf. (2018). Validity of Immunofluorescence Test for the Detection of Respiratory Viruses Causing Acute Lower Respiratory Tract Infection Among Under Five Children. International Journal of Microbiology and Biotechnology, 3(1), 7-10. https://doi.org/10.11648/j.ijmb.20180301.12
ACS Style
Rashida Akter Khanam; Md. Nazrul Islam; Shahina Tabassum; Ahmed Sharif; Md. Abdullah Yusuf. Validity of Immunofluorescence Test for the Detection of Respiratory Viruses Causing Acute Lower Respiratory Tract Infection Among Under Five Children. Int. J. Microbiol. Biotechnol. 2018, 3(1), 7-10. doi: 10.11648/j.ijmb.20180301.12
AMA Style
Rashida Akter Khanam, Md. Nazrul Islam, Shahina Tabassum, Ahmed Sharif, Md. Abdullah Yusuf. Validity of Immunofluorescence Test for the Detection of Respiratory Viruses Causing Acute Lower Respiratory Tract Infection Among Under Five Children. Int J Microbiol Biotechnol. 2018;3(1):7-10. doi: 10.11648/j.ijmb.20180301.12
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Download@article{10.11648/j.ijmb.20180301.12,
author = {Rashida Akter Khanam and Md. Nazrul Islam and Shahina Tabassum and Ahmed Sharif and Md. Abdullah Yusuf},
title = {Validity of Immunofluorescence Test for the Detection of Respiratory Viruses Causing Acute Lower Respiratory Tract Infection Among Under Five Children},
journal = {International Journal of Microbiology and Biotechnology},
volume = {3},
number = {1},
pages = {7-10},
doi = {10.11648/j.ijmb.20180301.12},
url = {https://doi.org/10.11648/j.ijmb.20180301.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20180301.12},
abstract = {Background: Respiratory viruses cause a variety of human infections, ranging from the common cold to life-threatening pneumonia. Over 200 strains of virus can cause respiratory disease. The majority of severe viral respiratory infections are caused by relatively few viruses, primarily parainfluenza virus types 1, 2 and 3, respiratory syncytial virus (RSV), influenza A and B viruses, and adenovirus. Objective: The purpose of this study was to see the validity of Immunofluorescence test for the detection of Respiratory viruses causing acute lower respiratory tract infection among under five children. Methodology: This cross sectional study was conducted in the Department of Virology at Bangabandhu Sheikh Mujib Medical University, Dhaka from July 2002 to June 2003 for a period of one year. The children with the age group of below five (5) years presented with the clinical manifestations of acute lower respiratory tract infection (ALRI) who were visited or were admitted to Dhaka Medical College Hospital (DMCH), Dhaka were selected as study population. Nasopharyngeal aspirates were collected. Viruses were detected by cell line culture and direct Immunoflorescence (DFA) method. Result: The study was carried out among 100 children aged from new born to 60 months with acute lower respiratory tract infection (ALRI). Highest rate (85.7%) of isolation was obtained among children between 0 to 24 months of age. There was a significant reduction in the number of cases in older children in 25 to 60 months of age group. The most common virus isolated from the under five children was respiratory syncytial virus which was 20(95.2%). Adenovirus was isolated in only 1(4.8%) case. No other viruses were found in this study. DFA method typically more rapid than the cell culture and also detect virus which has lost viability in transit. Culture methods on the other hand, are more favorable for detecting low titer of viable virus. In this study 17 samples are positive by cell culture and these are also positive by DFA. Total 21 samples are positive by DFA and among them 4 samples are negative. Conclusion: DFA is highly sensitive and specific for detection of respiratory viruses among the under-five children. Furthermore the accuracy of this test is also very high. Therefore it is recommended that the DFA test can be used for the detection of respiratory virus from the children presented with respiratory tract infection.},
year = {2018}
}
TY - JOUR T1 - Validity of Immunofluorescence Test for the Detection of Respiratory Viruses Causing Acute Lower Respiratory Tract Infection Among Under Five Children AU - Rashida Akter Khanam AU - Md. Nazrul Islam AU - Shahina Tabassum AU - Ahmed Sharif AU - Md. Abdullah Yusuf Y1 - 2018/03/02 PY - 2018 N1 - https://doi.org/10.11648/j.ijmb.20180301.12 DO - 10.11648/j.ijmb.20180301.12 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 7 EP - 10 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20180301.12 AB - Background: Respiratory viruses cause a variety of human infections, ranging from the common cold to life-threatening pneumonia. Over 200 strains of virus can cause respiratory disease. The majority of severe viral respiratory infections are caused by relatively few viruses, primarily parainfluenza virus types 1, 2 and 3, respiratory syncytial virus (RSV), influenza A and B viruses, and adenovirus. Objective: The purpose of this study was to see the validity of Immunofluorescence test for the detection of Respiratory viruses causing acute lower respiratory tract infection among under five children. Methodology: This cross sectional study was conducted in the Department of Virology at Bangabandhu Sheikh Mujib Medical University, Dhaka from July 2002 to June 2003 for a period of one year. The children with the age group of below five (5) years presented with the clinical manifestations of acute lower respiratory tract infection (ALRI) who were visited or were admitted to Dhaka Medical College Hospital (DMCH), Dhaka were selected as study population. Nasopharyngeal aspirates were collected. Viruses were detected by cell line culture and direct Immunoflorescence (DFA) method. Result: The study was carried out among 100 children aged from new born to 60 months with acute lower respiratory tract infection (ALRI). Highest rate (85.7%) of isolation was obtained among children between 0 to 24 months of age. There was a significant reduction in the number of cases in older children in 25 to 60 months of age group. The most common virus isolated from the under five children was respiratory syncytial virus which was 20(95.2%). Adenovirus was isolated in only 1(4.8%) case. No other viruses were found in this study. DFA method typically more rapid than the cell culture and also detect virus which has lost viability in transit. Culture methods on the other hand, are more favorable for detecting low titer of viable virus. In this study 17 samples are positive by cell culture and these are also positive by DFA. Total 21 samples are positive by DFA and among them 4 samples are negative. Conclusion: DFA is highly sensitive and specific for detection of respiratory viruses among the under-five children. Furthermore the accuracy of this test is also very high. Therefore it is recommended that the DFA test can be used for the detection of respiratory virus from the children presented with respiratory tract infection. VL - 3 IS - 1 ER -
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