The abuse and extensive use of antimicrobial agents by humans may increase resistant bacteria populations in the aquatic environment. The discharge of untreated wastewater into rivers and other non-point sources of pollution have led to the antibiotic resistant bacteria in the environment, particularly in surface waters. Studies on river water pollution and their implication to public health has been ongoing. Screening for multi-drug resistant bacterial status of Ikpoba River in Benin City, Nigeria was carried out using standard microbiological and physicochemical procedures. The bacteria isolated from the river water samples were E. coli, Salmonella sp, Vibrio sp, Staphylococcus aureus, and Streptococcus faecalis. The antibiotics susceptibility testing of the isolates revealed a multi-drug resistant status for Staphylococcus aureus and Streptococcus faecalis. The plasmid profile of these multi-drug resistant isolates was determined and results revealed that both isolates harboured plasmid of size 4.5kb. Antibiotic susceptibility of the isolates when cured of plasmid revealed loss of resistance to over 75% of the antibiotics they were originally resistant to. The microbial and physicochemical properties of the river showed that it is unfit for human consumption. The Plasmid mediated multidrug resistant status of some of the isolates is a threat to chemotherapy and is a cause for public health concern.
| Published in | International Journal of Microbiology and Biotechnology (Volume 3, Issue 2) |
| DOI | 10.11648/j.ijmb.20180302.11 |
| Page(s) | 31-35 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
River Water, Antibiotics, Pollution, Multi-drug Resistance, Plasmid
| [1] | Caro, L., Chruchwar, G. D., & Chandler, M, (1984). Study of plasmid replication in vivo. Methods in Microbiol. 17 72-122. |
| [2] | Magiorakos, A.P., Srinivasan, A., Carey, R.B., Carmeli, T., Falagas, M.E. (2012). Multidrug Resistance, Extensively drug-resistance and Pandrug-Resistance Bacteria in International Expert Proposal for Interim Standard Definitions for Acquired Resistance. Clinical Microbiology Infection 18: 269-281. |
| [3] | Messi, P., Guerrier, E., Bondi, M. (2005). Antibiotic Resistance and Antibacterial Activity in Heterotrophic Bacteria of Mineral Water Origin. Science Total Environment 346: 213-219. |
| [4] | Forsberg, K.J., Reyes, A., Wang, B., Selleck, E.M., Sommer, M.O. and Dantas, G. (2012). The Shared Antibiotic Resistance of Soil Bacteria and Human Pathogues. Science (337): 1110-1111. |
| [5] | Baquero, F., Martinez, J.L. and Canton, R. (2018). Antibiotic Resistance in Water Environments.Correct Opinion Biotechnology (19): 260-265. |
| [6] | Rizzo, L., Manaia, C., Merlin, C., Schwartz, T., Dagot, C., Ploy, M.C., Michael, I. and Fatta-Kassinos, D. (2013). Urban Wastewater Treatment Plants as Hotspots for Antibiotics Resistance Bacteria and Genes Spread into the Environment. A Review. Science Total Environment (447): 345-360. |
| [7] | Allenn, H.K., Donato, J., Wang, H.H., Cloud-Hansen, K.A., Davies, J., and Handelsman, J. (2010). Call of the Wide Antibiotic Resistance Genes in Natural Environments. National Revised Microbiology (8): 251-259. |
| [8] | Dapaola, A., Peeler, J. and Rodncky, G.E. (1995). Effects of Oxytetracychine-Mediated Feed On Antibiotic Resistance of Gram-Negative Bacteria in Catfish Ponds. Applied Environmental Microbiology (61): 2335-2340. |
| [9] | Zhang, X.X., Zhang, T. and Fang, H.H.P. (2009). Antibiotic Resistance Genes in Water Environment. Applied Microbiology Biotechnology (82): 397-414. |
| [10] | Ogbeibu, A.E. and Edutie, L.O. (2002). Effects of Brewery Effluents on the Water Quality and Rotifer. African Journal Environmental Pollution Health 1(2): 1-7. |
| [11] | Akpomie, O.O., Buzugbe, H.S. and Eze, P.M. (2014). Effect of brewery effluent on the microbiological quality of Ikpoba river and surrounding borehole water in Benin city, Nigeria. British Microbiology Research Journal 5(1): 76-82, 2015. ISSN: 2231-0886. |
| [12] | Ekhaise, F.O. and Anyansi, C.C. (2005). Influence of breweries effluent discharge on the microbiological and physicochemical quality of Ikpoba river, Nigeria. African Journal of Biotechnology 4 (10); 1062-1065. |
| [13] | Cheesbrough, M. (2006). District Laboratory Practice in Tropical Africa. University of Cambridge2nd Edition Update Part 2. Pp. 63-70. |
| [14] | Bauer, AW., Kirby, WM., Sheries, JC. and Turk, M. (1996).Antimicrobial sensitivity testing by agar diffusion method. Journal Clinical Pathology 45: 493-6. |
| [15] | Baver, A.W., Kirby, W.M.M., Sherris, J.C., Turck, M. (1966). Antibiotics susceptibility testing by a standardized singles disc method. American Journal of Clinical Pathology 145: 493-496 |
| [16] | Islam, M.T., M.R. Amin, S.M.R. Hoque and S.R. Alim, (2014). Microbial loads and association of enteropathogenic bacteria in ice-creams sold by street vendors at Dhaka city in Bangladesh. Int. J.Pharm. Sci. Res., 5: 2436-2440. |
| [17] | Winkler, U., Ruger,W. andWackernagew, L,. (1979). Bacterial, Phage and Molecular Genetics, Pp.-128. Berlin: Springer. |
| [18] | Akpe, A.R., Ekundayo, A.O. and Esumeh F.I. (2013). Degradation of Crude oil by bacteria: A role for plasmid-borne genes. Global Journal of Scientific Frontier Research Biological Science 13(Issue 6 Version 1.0): 21-26. |
| [19] | Dillon J. R. (1985). Recombinant DNA Methodology John Willey & Sons, Canada. Pp81–83. |
| [20] | Birnboim, H. C. and Doly, J. (1979). A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513-1523. |
| [21] | Sambrook J., Fritsch E. F., Maniatis T. (1989) Molecular cloning: laboratory manual, 2nd ed. Cold Spring Harbor Laboratory pres, Cold Spring Harbor, New York. ISBN 0-87969309-6. |
| [22] | Dapaola, A., Peeler, J. and Rodncky, G.E. (1995). Effects of Oxytetracychine-Mediated Feed On Antibiotic Resistance of Gram-Negative Bacteria in Catfish Ponds. Applied Environmental Microbiology (61): 2335-2340. |
| [23] | Forsberg, K.J., Reyes, A., Wang, B., Selleck, E.M., Sommer, M.O. and Dantas, G. (2012). TheShared Antibiotic Resistance of Soil Bacteria and Human Pathogens. Science (337): 1110-1111. |
| [24] | Enabulele, O. I. and Orikpete, A. V. (2009). Extended spectrum B-lactamase production in Gram –negative aerobic isolate from diabetic wounds. Nig. J. Microbiol. 23(1):1961-1969. |
| [25] | Esumeh, F. I., Akpe, A. R., Eguagie, O. E. (2009). Crude oil Degrading Capabilities of bacterial isolates from pawpaw (Carica papaya) and sweet orange (Citrus sinensis). A role for plasmid-mediated gene. Proceedings of the 1st International Conference, Workshop and exhibition on Biotechnologies for Improved Production of Oil and Gas in the Gulf of Guinea, held in Abuja, Nigeria 1. April 1-3. 2009. BIPOG3 -4-34. Pp. 1-7. |
| [26] | Craig, Maclean (2016). Antibiotic Resistance in Bacteria. Journal Nature, Ecology and Evolution. Pp. 33-46. |
APA Style
Akpe Azuka Romanus, Okwu Grace Ifeoma, Esumeh Frederick Ikechukwu, Femi, Imah Justus. (2018). Screening for Plasmid-Mediated Multidrug Resistant Bacteria in Ikpoba River Water Samples. International Journal of Microbiology and Biotechnology, 3(2), 31-35. https://doi.org/10.11648/j.ijmb.20180302.11
ACS Style
Akpe Azuka Romanus; Okwu Grace Ifeoma; Esumeh Frederick Ikechukwu; Femi; Imah Justus. Screening for Plasmid-Mediated Multidrug Resistant Bacteria in Ikpoba River Water Samples. Int. J. Microbiol. Biotechnol. 2018, 3(2), 31-35. doi: 10.11648/j.ijmb.20180302.11
AMA Style
Akpe Azuka Romanus, Okwu Grace Ifeoma, Esumeh Frederick Ikechukwu, Femi, Imah Justus. Screening for Plasmid-Mediated Multidrug Resistant Bacteria in Ikpoba River Water Samples. Int J Microbiol Biotechnol. 2018;3(2):31-35. doi: 10.11648/j.ijmb.20180302.11
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Download@article{10.11648/j.ijmb.20180302.11,
author = {Akpe Azuka Romanus and Okwu Grace Ifeoma and Esumeh Frederick Ikechukwu and Femi and Imah Justus},
title = {Screening for Plasmid-Mediated Multidrug Resistant Bacteria in Ikpoba River Water Samples},
journal = {International Journal of Microbiology and Biotechnology},
volume = {3},
number = {2},
pages = {31-35},
doi = {10.11648/j.ijmb.20180302.11},
url = {https://doi.org/10.11648/j.ijmb.20180302.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20180302.11},
abstract = {The abuse and extensive use of antimicrobial agents by humans may increase resistant bacteria populations in the aquatic environment. The discharge of untreated wastewater into rivers and other non-point sources of pollution have led to the antibiotic resistant bacteria in the environment, particularly in surface waters. Studies on river water pollution and their implication to public health has been ongoing. Screening for multi-drug resistant bacterial status of Ikpoba River in Benin City, Nigeria was carried out using standard microbiological and physicochemical procedures. The bacteria isolated from the river water samples were E. coli, Salmonella sp, Vibrio sp, Staphylococcus aureus, and Streptococcus faecalis. The antibiotics susceptibility testing of the isolates revealed a multi-drug resistant status for Staphylococcus aureus and Streptococcus faecalis. The plasmid profile of these multi-drug resistant isolates was determined and results revealed that both isolates harboured plasmid of size 4.5kb. Antibiotic susceptibility of the isolates when cured of plasmid revealed loss of resistance to over 75% of the antibiotics they were originally resistant to. The microbial and physicochemical properties of the river showed that it is unfit for human consumption. The Plasmid mediated multidrug resistant status of some of the isolates is a threat to chemotherapy and is a cause for public health concern.},
year = {2018}
}
TY - JOUR T1 - Screening for Plasmid-Mediated Multidrug Resistant Bacteria in Ikpoba River Water Samples AU - Akpe Azuka Romanus AU - Okwu Grace Ifeoma AU - Esumeh Frederick Ikechukwu AU - Femi AU - Imah Justus Y1 - 2018/05/07 PY - 2018 N1 - https://doi.org/10.11648/j.ijmb.20180302.11 DO - 10.11648/j.ijmb.20180302.11 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 31 EP - 35 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20180302.11 AB - The abuse and extensive use of antimicrobial agents by humans may increase resistant bacteria populations in the aquatic environment. The discharge of untreated wastewater into rivers and other non-point sources of pollution have led to the antibiotic resistant bacteria in the environment, particularly in surface waters. Studies on river water pollution and their implication to public health has been ongoing. Screening for multi-drug resistant bacterial status of Ikpoba River in Benin City, Nigeria was carried out using standard microbiological and physicochemical procedures. The bacteria isolated from the river water samples were E. coli, Salmonella sp, Vibrio sp, Staphylococcus aureus, and Streptococcus faecalis. The antibiotics susceptibility testing of the isolates revealed a multi-drug resistant status for Staphylococcus aureus and Streptococcus faecalis. The plasmid profile of these multi-drug resistant isolates was determined and results revealed that both isolates harboured plasmid of size 4.5kb. Antibiotic susceptibility of the isolates when cured of plasmid revealed loss of resistance to over 75% of the antibiotics they were originally resistant to. The microbial and physicochemical properties of the river showed that it is unfit for human consumption. The Plasmid mediated multidrug resistant status of some of the isolates is a threat to chemotherapy and is a cause for public health concern. VL - 3 IS - 2 ER -
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