In the present study, the probiotic potential of Lactobacillus isolates selected from fecal samples of farmyard chickens and ducks was scientifically validated for their use as alternatives to antibiotics in poultry. A total of 129 Lactobacillus isolates were characterized of which four produced inhibitory substances with antimicrobial activities. They were further identified on the basis of their carbohydrate fermentation profile and High-Resolution Melting analysis as Lactobacillus paracasei MW-37CGZ, Lactobacillus paracasei MW-38CGZ, Lactobacillus plantarum MW-48CGZ and Lactobacillus plantarum MW-18CGZ. The obtained results revealed that L. plantarum MW-18CGZ and L. paracasei MW-37CGZ showed strong antagonistic activities against human (nine) and zoonotic pathogens (eleven). The antimicrobial substance produced by L. plantarum MW-18CGZ was found to be proteinaceous, thus indicating that this substance may belong to a group of potent antimicrobial peptides produced by some microorganisms including lactic acid bacteria (LAB). Both viable and non-viable cells of the four isolates demonstrated good hydrophobicity in xylene with L. plantarum MW-48CGZ exhibiting higher hydrophobicity than other isolates (77.64±5.18%). They were susceptible to chloramphenicol, clindamycin, ampicilin and erythromycin with Minimum Inhibitory Concentration (MIC) below cut-off values established by the European Food Safety Authority (EFSA). Among the four Lactobacillus, L. plantarum MW-18CGZ and L. paracasei MW-37CGZ displayed high autoaggregation and coaggregation towards pathogens and all isolates survived in low-pH, high bile salt concentrations and none exhibited virulent factors. According to the obtained results, L. plantarum MW-18CGZ and L. paracasei MW-37CGZ could be considered as future biotherapeutic substitutes for antibiotics to reduce antibiotic residues in food derived from poultry as well as the generation and spread of antibiotic resistance.
| Published in | International Journal of Microbiology and Biotechnology (Volume 4, Issue 3) |
| DOI | 10.11648/j.ijmb.20190403.13 |
| Page(s) | 72-86 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Lactobacillus, Probiotics, Antimicrobial Activity, Antibiotics Resistance, Public Health
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APA Style
Raoul Emeric Guetiya Wadoum, Fonteh Anyangwe Florence, Kaktcham Pierre Marie, Ulrich Landry Bemmo Kamdem, Chancel Hector Momo Kenfack, et al. (2019). In Vitro Antimicrobial Characterization of Lactobacillus Isolates Towards Their Use as Probiotic Alternatives to Antibiotic Growth Promoters. International Journal of Microbiology and Biotechnology, 4(3), 72-86. https://doi.org/10.11648/j.ijmb.20190403.13
ACS Style
Raoul Emeric Guetiya Wadoum; Fonteh Anyangwe Florence; Kaktcham Pierre Marie; Ulrich Landry Bemmo Kamdem; Chancel Hector Momo Kenfack, et al. In Vitro Antimicrobial Characterization of Lactobacillus Isolates Towards Their Use as Probiotic Alternatives to Antibiotic Growth Promoters. Int. J. Microbiol. Biotechnol. 2019, 4(3), 72-86. doi: 10.11648/j.ijmb.20190403.13
AMA Style
Raoul Emeric Guetiya Wadoum, Fonteh Anyangwe Florence, Kaktcham Pierre Marie, Ulrich Landry Bemmo Kamdem, Chancel Hector Momo Kenfack, et al. In Vitro Antimicrobial Characterization of Lactobacillus Isolates Towards Their Use as Probiotic Alternatives to Antibiotic Growth Promoters. Int J Microbiol Biotechnol. 2019;4(3):72-86. doi: 10.11648/j.ijmb.20190403.13
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author = {Raoul Emeric Guetiya Wadoum and Fonteh Anyangwe Florence and Kaktcham Pierre Marie and Ulrich Landry Bemmo Kamdem and Chancel Hector Momo Kenfack and Foko Kouam Edith-Marius and Nathalie Nzekwa and Evina Horpa and Vittorio Colizzi and François Zambou Ngoufack},
title = {In Vitro Antimicrobial Characterization of Lactobacillus Isolates Towards Their Use as Probiotic Alternatives to Antibiotic Growth Promoters},
journal = {International Journal of Microbiology and Biotechnology},
volume = {4},
number = {3},
pages = {72-86},
doi = {10.11648/j.ijmb.20190403.13},
url = {https://doi.org/10.11648/j.ijmb.20190403.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20190403.13},
abstract = {In the present study, the probiotic potential of Lactobacillus isolates selected from fecal samples of farmyard chickens and ducks was scientifically validated for their use as alternatives to antibiotics in poultry. A total of 129 Lactobacillus isolates were characterized of which four produced inhibitory substances with antimicrobial activities. They were further identified on the basis of their carbohydrate fermentation profile and High-Resolution Melting analysis as Lactobacillus paracasei MW-37CGZ, Lactobacillus paracasei MW-38CGZ, Lactobacillus plantarum MW-48CGZ and Lactobacillus plantarum MW-18CGZ. The obtained results revealed that L. plantarum MW-18CGZ and L. paracasei MW-37CGZ showed strong antagonistic activities against human (nine) and zoonotic pathogens (eleven). The antimicrobial substance produced by L. plantarum MW-18CGZ was found to be proteinaceous, thus indicating that this substance may belong to a group of potent antimicrobial peptides produced by some microorganisms including lactic acid bacteria (LAB). Both viable and non-viable cells of the four isolates demonstrated good hydrophobicity in xylene with L. plantarum MW-48CGZ exhibiting higher hydrophobicity than other isolates (77.64±5.18%). They were susceptible to chloramphenicol, clindamycin, ampicilin and erythromycin with Minimum Inhibitory Concentration (MIC) below cut-off values established by the European Food Safety Authority (EFSA). Among the four Lactobacillus, L. plantarum MW-18CGZ and L. paracasei MW-37CGZ displayed high autoaggregation and coaggregation towards pathogens and all isolates survived in low-pH, high bile salt concentrations and none exhibited virulent factors. According to the obtained results, L. plantarum MW-18CGZ and L. paracasei MW-37CGZ could be considered as future biotherapeutic substitutes for antibiotics to reduce antibiotic residues in food derived from poultry as well as the generation and spread of antibiotic resistance.},
year = {2019}
}
TY - JOUR T1 - In Vitro Antimicrobial Characterization of Lactobacillus Isolates Towards Their Use as Probiotic Alternatives to Antibiotic Growth Promoters AU - Raoul Emeric Guetiya Wadoum AU - Fonteh Anyangwe Florence AU - Kaktcham Pierre Marie AU - Ulrich Landry Bemmo Kamdem AU - Chancel Hector Momo Kenfack AU - Foko Kouam Edith-Marius AU - Nathalie Nzekwa AU - Evina Horpa AU - Vittorio Colizzi AU - François Zambou Ngoufack Y1 - 2019/08/05 PY - 2019 N1 - https://doi.org/10.11648/j.ijmb.20190403.13 DO - 10.11648/j.ijmb.20190403.13 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 72 EP - 86 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20190403.13 AB - In the present study, the probiotic potential of Lactobacillus isolates selected from fecal samples of farmyard chickens and ducks was scientifically validated for their use as alternatives to antibiotics in poultry. A total of 129 Lactobacillus isolates were characterized of which four produced inhibitory substances with antimicrobial activities. They were further identified on the basis of their carbohydrate fermentation profile and High-Resolution Melting analysis as Lactobacillus paracasei MW-37CGZ, Lactobacillus paracasei MW-38CGZ, Lactobacillus plantarum MW-48CGZ and Lactobacillus plantarum MW-18CGZ. The obtained results revealed that L. plantarum MW-18CGZ and L. paracasei MW-37CGZ showed strong antagonistic activities against human (nine) and zoonotic pathogens (eleven). The antimicrobial substance produced by L. plantarum MW-18CGZ was found to be proteinaceous, thus indicating that this substance may belong to a group of potent antimicrobial peptides produced by some microorganisms including lactic acid bacteria (LAB). Both viable and non-viable cells of the four isolates demonstrated good hydrophobicity in xylene with L. plantarum MW-48CGZ exhibiting higher hydrophobicity than other isolates (77.64±5.18%). They were susceptible to chloramphenicol, clindamycin, ampicilin and erythromycin with Minimum Inhibitory Concentration (MIC) below cut-off values established by the European Food Safety Authority (EFSA). Among the four Lactobacillus, L. plantarum MW-18CGZ and L. paracasei MW-37CGZ displayed high autoaggregation and coaggregation towards pathogens and all isolates survived in low-pH, high bile salt concentrations and none exhibited virulent factors. According to the obtained results, L. plantarum MW-18CGZ and L. paracasei MW-37CGZ could be considered as future biotherapeutic substitutes for antibiotics to reduce antibiotic residues in food derived from poultry as well as the generation and spread of antibiotic resistance. VL - 4 IS - 3 ER -
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