A total of eleven phosphate solubilizing bacteria were isolated from rhizosphere soil of tomato (Solanum lycopersicum) plants. Isolates designated as PSB1, PSB2, PSB4, PSB5 and PSB7 were selected out of eleven isolates based on their clear zone diameters. They were characterized to generic level and found to belong to Pseudomonas spp. Tricalcium phosphate (TCP), rock phosphate (RP) and bone phosphate (BP) solubilizing efficiency of these isolates was studied in the laboratory at 5, 10, and 15 days of incubation. All bacterial isolates solubilized significantly (P< 0.05) higher amounts of TCP over uninoculated control. The highest P was release upon 5 days of incubation was recorded from the isolate PSB1 with 7.64 mg/50ml in the medium and the least P release was recorded by isolates PSB4 and PSB5 with a P content of 4.79 mg /50ml. At 10th day of incubation, the highest P release was recorded by isolates PSB2 (8.19 mg/50ml), PSB1 (8.10mg/ 50ml). At 15th days of incubation, the highest P release was recorded by isolate PSB7 (11.77 mg/ 50ml) and PSB2 (11.33 mg/ 50ml) and the least P released was recorded by isolate PSB5 (5.44 mg/ 50ml)). Under RP solubilization, the p released from all isolates upon 5 days of incubation was not significant as compared to the control. At 10th day of incubation, the highest P release was recorded by isolates PSB5 (5.56 mg/50ml), PSB7 (5.02 mg/ 50ml) and the least P release was observed by isolate PSB4 (3.46mg/g). At 15th days of incubation, the highest P release was recorded by isolate PSB7 (7.928 mg/ 50ml) and the least P release was recorded by isolate PSB4 (4.025mg/ 50ml). Regarding BP, the p released from all isolates except isolate PSB7 (3.020mg /50ml) upon 5 days of incubation was not significant as compared to the control. At 10th day of incubation, the highest P release was recorded by isolates PSB5 (7.37 mg/50ml), PSB7 (7.025 mg/ 50ml) and the least P released was observed by isolate PSB2 (5.47 mg/50ml). At 20th days of incubation, the highest P release was recorded by isolate PSB7 (11.09 mg/ 50ml) and the least P release was recorded by isolate PSB4 (9.06 mg/ 50ml). So, as conclusion all Psedomonas isolates were able to release P from both inorganic and organic phosphate sources.
| Published in | International Journal of Microbiology and Biotechnology (Volume 4, Issue 3) |
| DOI | 10.11648/j.ijmb.20190403.14 |
| Page(s) | 87-93 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Biofertilizers, Clear Zone, Phosphorus, Solubilization Efficiency
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APA Style
Lamenew Fenta, Fassil Assefa. (2019). Isolation of Pseudomonas with the Ability to Solublize Phosphate from Inorganic and Organic Phosphate Sources. International Journal of Microbiology and Biotechnology, 4(3), 87-93. https://doi.org/10.11648/j.ijmb.20190403.14
ACS Style
Lamenew Fenta; Fassil Assefa. Isolation of Pseudomonas with the Ability to Solublize Phosphate from Inorganic and Organic Phosphate Sources. Int. J. Microbiol. Biotechnol. 2019, 4(3), 87-93. doi: 10.11648/j.ijmb.20190403.14
AMA Style
Lamenew Fenta, Fassil Assefa. Isolation of Pseudomonas with the Ability to Solublize Phosphate from Inorganic and Organic Phosphate Sources. Int J Microbiol Biotechnol. 2019;4(3):87-93. doi: 10.11648/j.ijmb.20190403.14
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Download@article{10.11648/j.ijmb.20190403.14,
author = {Lamenew Fenta and Fassil Assefa},
title = {Isolation of Pseudomonas with the Ability to Solublize Phosphate from Inorganic and Organic Phosphate Sources},
journal = {International Journal of Microbiology and Biotechnology},
volume = {4},
number = {3},
pages = {87-93},
doi = {10.11648/j.ijmb.20190403.14},
url = {https://doi.org/10.11648/j.ijmb.20190403.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20190403.14},
abstract = {A total of eleven phosphate solubilizing bacteria were isolated from rhizosphere soil of tomato (Solanum lycopersicum) plants. Isolates designated as PSB1, PSB2, PSB4, PSB5 and PSB7 were selected out of eleven isolates based on their clear zone diameters. They were characterized to generic level and found to belong to Pseudomonas spp. Tricalcium phosphate (TCP), rock phosphate (RP) and bone phosphate (BP) solubilizing efficiency of these isolates was studied in the laboratory at 5, 10, and 15 days of incubation. All bacterial isolates solubilized significantly (Pth day of incubation, the highest P release was recorded by isolates PSB5 (5.56 mg/50ml), PSB7 (5.02 mg/ 50ml) and the least P release was observed by isolate PSB4 (3.46mg/g). At 15th days of incubation, the highest P release was recorded by isolate PSB7 (7.928 mg/ 50ml) and the least P release was recorded by isolate PSB4 (4.025mg/ 50ml). Regarding BP, the p released from all isolates except isolate PSB7 (3.020mg /50ml) upon 5 days of incubation was not significant as compared to the control. At 10th day of incubation, the highest P release was recorded by isolates PSB5 (7.37 mg/50ml), PSB7 (7.025 mg/ 50ml) and the least P released was observed by isolate PSB2 (5.47 mg/50ml). At 20th days of incubation, the highest P release was recorded by isolate PSB7 (11.09 mg/ 50ml) and the least P release was recorded by isolate PSB4 (9.06 mg/ 50ml). So, as conclusion all Psedomonas isolates were able to release P from both inorganic and organic phosphate sources.},
year = {2019}
}
TY - JOUR T1 - Isolation of Pseudomonas with the Ability to Solublize Phosphate from Inorganic and Organic Phosphate Sources AU - Lamenew Fenta AU - Fassil Assefa Y1 - 2019/09/05 PY - 2019 N1 - https://doi.org/10.11648/j.ijmb.20190403.14 DO - 10.11648/j.ijmb.20190403.14 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 87 EP - 93 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20190403.14 AB - A total of eleven phosphate solubilizing bacteria were isolated from rhizosphere soil of tomato (Solanum lycopersicum) plants. Isolates designated as PSB1, PSB2, PSB4, PSB5 and PSB7 were selected out of eleven isolates based on their clear zone diameters. They were characterized to generic level and found to belong to Pseudomonas spp. Tricalcium phosphate (TCP), rock phosphate (RP) and bone phosphate (BP) solubilizing efficiency of these isolates was studied in the laboratory at 5, 10, and 15 days of incubation. All bacterial isolates solubilized significantly (Pth day of incubation, the highest P release was recorded by isolates PSB5 (5.56 mg/50ml), PSB7 (5.02 mg/ 50ml) and the least P release was observed by isolate PSB4 (3.46mg/g). At 15th days of incubation, the highest P release was recorded by isolate PSB7 (7.928 mg/ 50ml) and the least P release was recorded by isolate PSB4 (4.025mg/ 50ml). Regarding BP, the p released from all isolates except isolate PSB7 (3.020mg /50ml) upon 5 days of incubation was not significant as compared to the control. At 10th day of incubation, the highest P release was recorded by isolates PSB5 (7.37 mg/50ml), PSB7 (7.025 mg/ 50ml) and the least P released was observed by isolate PSB2 (5.47 mg/50ml). At 20th days of incubation, the highest P release was recorded by isolate PSB7 (11.09 mg/ 50ml) and the least P release was recorded by isolate PSB4 (9.06 mg/ 50ml). So, as conclusion all Psedomonas isolates were able to release P from both inorganic and organic phosphate sources. VL - 4 IS - 3 ER -
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