Limonene is essential oil isolated from pericarp of C. sinensis it is well known for its antimicrobial and antiseptic activities. Limonene has many medicinal properties and is widely used for the treatment of stomach aches, breast cancer, and as tonic for the digestive system, immune system and skin. It is also used to treat and prevent vitamin deficiencies, colds, flu, and typhoid fever, and to help fight viral and bacterial infections. The aim of this work was to determine the composition, antioxidant and antibacterial properties of limonene from pericarp of C. sinensis. Limonene from C. sinensis pericarps were extracted using steam distillation method. Phytochemical screening of the extracts revealed the presence of terpenoids and limonene in the extracts while GC/MS analysis showed highest percentage of limonene (96%). Result of antioxidant activity determination showed that limonene demonstrated strong scavenging activity (92.42%). Antimicrobial activity of the limonene extracts was evaluated by agar well method against S. aureus, S. pyogenes, P. aeruginosa, S. typhi while MIC and MBC were determined using the agar well dilution technique. Result showed that limonene extract exhibited an MIC of 200 mg/ml and MBC range of 300-400 mg/ml against all the isolates. The antibacterial effects of the extracts suggest their possible inclusion as candidate agents for development of chemotherapeutic agents against infection caused by the bacterial pathogen s tested.
| Published in | International Journal of Microbiology and Biotechnology (Volume 5, Issue 1) |
| DOI | 10.11648/j.ijmb.20200501.14 |
| Page(s) | 22-27 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Limonene, GS-MS Analysis, Phytochemical, Antioxidant, Antibacterial Activity
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APA Style
James Hamuel Doughari, Musa Jamila Bazza. (2020). Phytochemistry, GC-MS Analysis, Antioxidant and Antibacterial Potentials of Limonene Isolated from Pericarp of Citrus sinensis. International Journal of Microbiology and Biotechnology, 5(1), 22-27. https://doi.org/10.11648/j.ijmb.20200501.14
ACS Style
James Hamuel Doughari; Musa Jamila Bazza. Phytochemistry, GC-MS Analysis, Antioxidant and Antibacterial Potentials of Limonene Isolated from Pericarp of Citrus sinensis. Int. J. Microbiol. Biotechnol. 2020, 5(1), 22-27. doi: 10.11648/j.ijmb.20200501.14
AMA Style
James Hamuel Doughari, Musa Jamila Bazza. Phytochemistry, GC-MS Analysis, Antioxidant and Antibacterial Potentials of Limonene Isolated from Pericarp of Citrus sinensis. Int J Microbiol Biotechnol. 2020;5(1):22-27. doi: 10.11648/j.ijmb.20200501.14
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Download@article{10.11648/j.ijmb.20200501.14,
author = {James Hamuel Doughari and Musa Jamila Bazza},
title = {Phytochemistry, GC-MS Analysis, Antioxidant and Antibacterial Potentials of Limonene Isolated from Pericarp of Citrus sinensis},
journal = {International Journal of Microbiology and Biotechnology},
volume = {5},
number = {1},
pages = {22-27},
doi = {10.11648/j.ijmb.20200501.14},
url = {https://doi.org/10.11648/j.ijmb.20200501.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20200501.14},
abstract = {Limonene is essential oil isolated from pericarp of C. sinensis it is well known for its antimicrobial and antiseptic activities. Limonene has many medicinal properties and is widely used for the treatment of stomach aches, breast cancer, and as tonic for the digestive system, immune system and skin. It is also used to treat and prevent vitamin deficiencies, colds, flu, and typhoid fever, and to help fight viral and bacterial infections. The aim of this work was to determine the composition, antioxidant and antibacterial properties of limonene from pericarp of C. sinensis. Limonene from C. sinensis pericarps were extracted using steam distillation method. Phytochemical screening of the extracts revealed the presence of terpenoids and limonene in the extracts while GC/MS analysis showed highest percentage of limonene (96%). Result of antioxidant activity determination showed that limonene demonstrated strong scavenging activity (92.42%). Antimicrobial activity of the limonene extracts was evaluated by agar well method against S. aureus, S. pyogenes, P. aeruginosa, S. typhi while MIC and MBC were determined using the agar well dilution technique. Result showed that limonene extract exhibited an MIC of 200 mg/ml and MBC range of 300-400 mg/ml against all the isolates. The antibacterial effects of the extracts suggest their possible inclusion as candidate agents for development of chemotherapeutic agents against infection caused by the bacterial pathogen s tested.},
year = {2020}
}
TY - JOUR T1 - Phytochemistry, GC-MS Analysis, Antioxidant and Antibacterial Potentials of Limonene Isolated from Pericarp of Citrus sinensis AU - James Hamuel Doughari AU - Musa Jamila Bazza Y1 - 2020/02/19 PY - 2020 N1 - https://doi.org/10.11648/j.ijmb.20200501.14 DO - 10.11648/j.ijmb.20200501.14 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 22 EP - 27 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20200501.14 AB - Limonene is essential oil isolated from pericarp of C. sinensis it is well known for its antimicrobial and antiseptic activities. Limonene has many medicinal properties and is widely used for the treatment of stomach aches, breast cancer, and as tonic for the digestive system, immune system and skin. It is also used to treat and prevent vitamin deficiencies, colds, flu, and typhoid fever, and to help fight viral and bacterial infections. The aim of this work was to determine the composition, antioxidant and antibacterial properties of limonene from pericarp of C. sinensis. Limonene from C. sinensis pericarps were extracted using steam distillation method. Phytochemical screening of the extracts revealed the presence of terpenoids and limonene in the extracts while GC/MS analysis showed highest percentage of limonene (96%). Result of antioxidant activity determination showed that limonene demonstrated strong scavenging activity (92.42%). Antimicrobial activity of the limonene extracts was evaluated by agar well method against S. aureus, S. pyogenes, P. aeruginosa, S. typhi while MIC and MBC were determined using the agar well dilution technique. Result showed that limonene extract exhibited an MIC of 200 mg/ml and MBC range of 300-400 mg/ml against all the isolates. The antibacterial effects of the extracts suggest their possible inclusion as candidate agents for development of chemotherapeutic agents against infection caused by the bacterial pathogen s tested. VL - 5 IS - 1 ER -
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