A κ-carrageenan degrading bacterium was isolated from decaying red seaweeds. Based on biochemical characterization and 16S rRNA sequence the isolate was identified as Alteromonas macleodii KS62. To the best of our knowledge, this is the first study reporting κ-carrageenan degrading bacterium of species Alteromonas macleodii. κ-carrageenase enzyme production of 1.6U/ml (12.5U/mg protein) was obtained when Alteromonas macleodii KS62 was grown in a medium containing κ-carrageenan as carbon source. The κ-carrageenase production further increased to 2.9U/ml (14.1U/mg protein) when Alteromonas macleodii KS62 was grown in a medium supplemented with Kappaphycus red seaweed powder. This the first report on κ-carrageenase production using red seaweed biomass as substrate. Efficient κ-carrageenase production establishes the potential of red seaweed biomass to serve as a low cost substrate for economic production of κ-carrageenase enzymes. Enzymatic hydrolysis of κ-carrageenan was studied. κ-carrageenan tetrasaccharide and hexasaccharide were found to be the major end products of enzymatic hydrolysis.
| Published in | International Journal of Microbiology and Biotechnology (Volume 4, Issue 4) |
| DOI | 10.11648/j.ijmb.20190404.13 |
| Page(s) | 128-132 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Alteromonas macleodii, Κ-carrageenan, Κ-carrageenase, Enzyme, Red Seaweeds, Kappaphycus
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APA Style
Prajakta Naval, Chandra Sainathan. (2019). Alteromonas macleodii KS62 (MTCC 12606): A Novel κ-Carrageenase Producing Microorganism. International Journal of Microbiology and Biotechnology, 4(4), 128-132. https://doi.org/10.11648/j.ijmb.20190404.13
ACS Style
Prajakta Naval; Chandra Sainathan. Alteromonas macleodii KS62 (MTCC 12606): A Novel κ-Carrageenase Producing Microorganism. Int. J. Microbiol. Biotechnol. 2019, 4(4), 128-132. doi: 10.11648/j.ijmb.20190404.13
AMA Style
Prajakta Naval, Chandra Sainathan. Alteromonas macleodii KS62 (MTCC 12606): A Novel κ-Carrageenase Producing Microorganism. Int J Microbiol Biotechnol. 2019;4(4):128-132. doi: 10.11648/j.ijmb.20190404.13
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Download@article{10.11648/j.ijmb.20190404.13,
author = {Prajakta Naval and Chandra Sainathan},
title = {Alteromonas macleodii KS62 (MTCC 12606): A Novel κ-Carrageenase Producing Microorganism},
journal = {International Journal of Microbiology and Biotechnology},
volume = {4},
number = {4},
pages = {128-132},
doi = {10.11648/j.ijmb.20190404.13},
url = {https://doi.org/10.11648/j.ijmb.20190404.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20190404.13},
abstract = {A κ-carrageenan degrading bacterium was isolated from decaying red seaweeds. Based on biochemical characterization and 16S rRNA sequence the isolate was identified as Alteromonas macleodii KS62. To the best of our knowledge, this is the first study reporting κ-carrageenan degrading bacterium of species Alteromonas macleodii. κ-carrageenase enzyme production of 1.6U/ml (12.5U/mg protein) was obtained when Alteromonas macleodii KS62 was grown in a medium containing κ-carrageenan as carbon source. The κ-carrageenase production further increased to 2.9U/ml (14.1U/mg protein) when Alteromonas macleodii KS62 was grown in a medium supplemented with Kappaphycus red seaweed powder. This the first report on κ-carrageenase production using red seaweed biomass as substrate. Efficient κ-carrageenase production establishes the potential of red seaweed biomass to serve as a low cost substrate for economic production of κ-carrageenase enzymes. Enzymatic hydrolysis of κ-carrageenan was studied. κ-carrageenan tetrasaccharide and hexasaccharide were found to be the major end products of enzymatic hydrolysis.},
year = {2019}
}
TY - JOUR T1 - Alteromonas macleodii KS62 (MTCC 12606): A Novel κ-Carrageenase Producing Microorganism AU - Prajakta Naval AU - Chandra Sainathan Y1 - 2019/10/23 PY - 2019 N1 - https://doi.org/10.11648/j.ijmb.20190404.13 DO - 10.11648/j.ijmb.20190404.13 T2 - International Journal of Microbiology and Biotechnology JF - International Journal of Microbiology and Biotechnology JO - International Journal of Microbiology and Biotechnology SP - 128 EP - 132 PB - Science Publishing Group SN - 2578-9686 UR - https://doi.org/10.11648/j.ijmb.20190404.13 AB - A κ-carrageenan degrading bacterium was isolated from decaying red seaweeds. Based on biochemical characterization and 16S rRNA sequence the isolate was identified as Alteromonas macleodii KS62. To the best of our knowledge, this is the first study reporting κ-carrageenan degrading bacterium of species Alteromonas macleodii. κ-carrageenase enzyme production of 1.6U/ml (12.5U/mg protein) was obtained when Alteromonas macleodii KS62 was grown in a medium containing κ-carrageenan as carbon source. The κ-carrageenase production further increased to 2.9U/ml (14.1U/mg protein) when Alteromonas macleodii KS62 was grown in a medium supplemented with Kappaphycus red seaweed powder. This the first report on κ-carrageenase production using red seaweed biomass as substrate. Efficient κ-carrageenase production establishes the potential of red seaweed biomass to serve as a low cost substrate for economic production of κ-carrageenase enzymes. Enzymatic hydrolysis of κ-carrageenan was studied. κ-carrageenan tetrasaccharide and hexasaccharide were found to be the major end products of enzymatic hydrolysis. VL - 4 IS - 4 ER -
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