Analysis of Population Genetics of the Endangered Nile Pufferfish <i>Tetraodon lineatus</i> (Linnaeus, 1758) in the Upper Egyptian River Nile

Khaled Mohammed-Geba; Aldoushy A. Mahdy; Ahmed S. A. Eissa; Alaa G. M. Osman

doi:doi:10.11648/j.ijee.20160102.15

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Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile

Khaled Mohammed-Geba, Aldoushy A. Mahdy, Ahmed S. A. Eissa, Alaa G. M. Osman

Published in International Journal of Ecotoxicology and Ecobiology (Volume 1, Issue 2)

Received: 24 May 2016 Accepted: 12 July 2016 Published: 26 August 2016

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Abstract

Genetic population analyses for Nile fishes are very scarce. Nile pufferfish Tetraodon lineatus (Linnaeus, 1758), is a widely-distributed freshwater fish, with no known major widespread threats. It has been classed as 'Endangered'. Little is known concerning its biology and genetics in Egypt. Hence, this work was designed to study the genetic diversity and conservation status of T. lineatus for the first time in Egypt and Africa. DNA barcoding was carried out through PCR-amplification and sequencing of the cytochrome c oxidase gene barcode 5´ region of forty-five samples obtained from three different localities in Upper Egypt. Only three haplotypes could be characterized in all samples. The other population analyses showed clear population loss of extension and potential bottleneck, what may explain the severe drop of species records in the Northern areas of the Nile. Phylogenetic analysis exhibited the monophyletic origin T. lineatus and other African freshwater pufferfishes, more probably as descendants from an Indo-West Pacific ancestor. We highly recommend the fulfilling of more studies concerning the biology, ecology, and genetics of the species as major steps towards its proper conservation and understanding of its adaptation to different natural and man-made constraints in the River Nile system.

Published in	International Journal of Ecotoxicology and Ecobiology (Volume 1, Issue 2)
DOI	10.11648/j.ijee.20160102.15
Page(s)	60-66
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Africa, COI, PCR, Population Genetics, Pufferfish, Tetraodon lineatus

References

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Khaled Mohammed-Geba, Aldoushy A. Mahdy, Ahmed S. A. Eissa, Alaa G. M. Osman. (2016). Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile. International Journal of Ecotoxicology and Ecobiology, 1(2), 60-66. https://doi.org/10.11648/j.ijee.20160102.15

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Khaled Mohammed-Geba; Aldoushy A. Mahdy; Ahmed S. A. Eissa; Alaa G. M. Osman. Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile. Int. J. Ecotoxicol. Ecobiol. 2016, 1(2), 60-66. doi: 10.11648/j.ijee.20160102.15

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Khaled Mohammed-Geba, Aldoushy A. Mahdy, Ahmed S. A. Eissa, Alaa G. M. Osman. Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile. Int J Ecotoxicol Ecobiol. 2016;1(2):60-66. doi: 10.11648/j.ijee.20160102.15

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@article{10.11648/j.ijee.20160102.15,
  author = {Khaled Mohammed-Geba and Aldoushy A. Mahdy and Ahmed S. A. Eissa and Alaa G. M. Osman},
  title = {Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile},
  journal = {International Journal of Ecotoxicology and Ecobiology},
  volume = {1},
  number = {2},
  pages = {60-66},
  doi = {10.11648/j.ijee.20160102.15},
  url = {https://doi.org/10.11648/j.ijee.20160102.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijee.20160102.15},
  abstract = {Genetic population analyses for Nile fishes are very scarce. Nile pufferfish Tetraodon lineatus (Linnaeus, 1758), is a widely-distributed freshwater fish, with no known major widespread threats. It has been classed as 'Endangered'. Little is known concerning its biology and genetics in Egypt. Hence, this work was designed to study the genetic diversity and conservation status of T. lineatus for the first time in Egypt and Africa. DNA barcoding was carried out through PCR-amplification and sequencing of the cytochrome c oxidase gene barcode 5´ region of forty-five samples obtained from three different localities in Upper Egypt. Only three haplotypes could be characterized in all samples. The other population analyses showed clear population loss of extension and potential bottleneck, what may explain the severe drop of species records in the Northern areas of the Nile. Phylogenetic analysis exhibited the monophyletic origin T. lineatus and other African freshwater pufferfishes, more probably as descendants from an Indo-West Pacific ancestor. We highly recommend the fulfilling of more studies concerning the biology, ecology, and genetics of the species as major steps towards its proper conservation and understanding of its adaptation to different natural and man-made constraints in the River Nile system.},
 year = {2016}
}

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TY - JOUR
T1 - Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile
AU - Khaled Mohammed-Geba
AU - Aldoushy A. Mahdy
AU - Ahmed S. A. Eissa
AU - Alaa G. M. Osman
Y1 - 2016/08/26
PY - 2016
N1 - https://doi.org/10.11648/j.ijee.20160102.15
DO - 10.11648/j.ijee.20160102.15
T2 - International Journal of Ecotoxicology and Ecobiology
JF - International Journal of Ecotoxicology and Ecobiology
JO - International Journal of Ecotoxicology and Ecobiology
SP - 60
EP - 66
PB - Science Publishing Group
SN - 2575-1735
UR - https://doi.org/10.11648/j.ijee.20160102.15
AB - Genetic population analyses for Nile fishes are very scarce. Nile pufferfish Tetraodon lineatus (Linnaeus, 1758), is a widely-distributed freshwater fish, with no known major widespread threats. It has been classed as 'Endangered'. Little is known concerning its biology and genetics in Egypt. Hence, this work was designed to study the genetic diversity and conservation status of T. lineatus for the first time in Egypt and Africa. DNA barcoding was carried out through PCR-amplification and sequencing of the cytochrome c oxidase gene barcode 5´ region of forty-five samples obtained from three different localities in Upper Egypt. Only three haplotypes could be characterized in all samples. The other population analyses showed clear population loss of extension and potential bottleneck, what may explain the severe drop of species records in the Northern areas of the Nile. Phylogenetic analysis exhibited the monophyletic origin T. lineatus and other African freshwater pufferfishes, more probably as descendants from an Indo-West Pacific ancestor. We highly recommend the fulfilling of more studies concerning the biology, ecology, and genetics of the species as major steps towards its proper conservation and understanding of its adaptation to different natural and man-made constraints in the River Nile system.
VL - 1
IS - 2
ER -

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Author Information

Khaled Mohammed-Geba

Department of Zoology, Faculty of Science, Menoufia University, 32511 Shebin El-Kom, Egypt
Aldoushy A. Mahdy

Department of Zoology, Faculty of Science, Al-Azhar University, Assiut branch, 71524 Assiut, Egypt
Ahmed S. A. Eissa

Department of Fisheries, Organization of World Fish, Aswan, Egypt
Alaa G. M. Osman

Department of Zoology, Faculty of Science, Al-Azhar University, Assiut branch, 71524 Assiut, Egypt

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Khaled Mohammed-Geba, Aldoushy A. Mahdy, Ahmed S. A. Eissa, Alaa G. M. Osman. (2016). Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile. International Journal of Ecotoxicology and Ecobiology, 1(2), 60-66. https://doi.org/10.11648/j.ijee.20160102.15

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Khaled Mohammed-Geba; Aldoushy A. Mahdy; Ahmed S. A. Eissa; Alaa G. M. Osman. Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile. Int. J. Ecotoxicol. Ecobiol. 2016, 1(2), 60-66. doi: 10.11648/j.ijee.20160102.15

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AMA Style

Khaled Mohammed-Geba, Aldoushy A. Mahdy, Ahmed S. A. Eissa, Alaa G. M. Osman. Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile. Int J Ecotoxicol Ecobiol. 2016;1(2):60-66. doi: 10.11648/j.ijee.20160102.15

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@article{10.11648/j.ijee.20160102.15,
  author = {Khaled Mohammed-Geba and Aldoushy A. Mahdy and Ahmed S. A. Eissa and Alaa G. M. Osman},
  title = {Analysis of Population Genetics of the Endangered Nile Pufferfish Tetraodon lineatus (Linnaeus, 1758) in the Upper Egyptian River Nile},
  journal = {International Journal of Ecotoxicology and Ecobiology},
  volume = {1},
  number = {2},
  pages = {60-66},
  doi = {10.11648/j.ijee.20160102.15},
  url = {https://doi.org/10.11648/j.ijee.20160102.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijee.20160102.15},
  abstract = {Genetic population analyses for Nile fishes are very scarce. Nile pufferfish Tetraodon lineatus (Linnaeus, 1758), is a widely-distributed freshwater fish, with no known major widespread threats. It has been classed as 'Endangered'. Little is known concerning its biology and genetics in Egypt. Hence, this work was designed to study the genetic diversity and conservation status of T. lineatus for the first time in Egypt and Africa. DNA barcoding was carried out through PCR-amplification and sequencing of the cytochrome c oxidase gene barcode 5´ region of forty-five samples obtained from three different localities in Upper Egypt. Only three haplotypes could be characterized in all samples. The other population analyses showed clear population loss of extension and potential bottleneck, what may explain the severe drop of species records in the Northern areas of the Nile. Phylogenetic analysis exhibited the monophyletic origin T. lineatus and other African freshwater pufferfishes, more probably as descendants from an Indo-West Pacific ancestor. We highly recommend the fulfilling of more studies concerning the biology, ecology, and genetics of the species as major steps towards its proper conservation and understanding of its adaptation to different natural and man-made constraints in the River Nile system.},
 year = {2016}
}

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