A field study on Streptococcus infection as a major cause for epidemic infections in Nile Tilapia aquaculture in Kafr El-Shikh Governorate was conducted. This study was carried out to study the gross lesions and post mortem finding of Streptococcsis in Nile Tilapia. Streptococcus was isolated, then identified by Electron Microscopy (Scanning Electron Microscope (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscope (TEM)) to investigate the phenotypic characteristics, ultra-structure of the bacterial cells. Clinical signs of the infected fish showed presence of hemorrhagic patches on different external body regions, ascitis and exophthalmia. Post mortem examinations revealed presence of inflammation of the liver, spleen and kidney. Microbial identification by Gram stain showed Gram positive cocci in shape arranged in chains and the biochemical tests displayed negative catalase and oxidase tests. Electron Microscopy (SEM, EDAX & TEM) confirmed these results and showed the clear cocci shape of streptococcus species and the arrangement of the chains with a bacterial average length of 700 nm. The EDAX results revealed that the elemental structure of the bacterial sample were C,N,O with the concentration 46.93%, 16.61% and 36.46%, respectively.
| Published in | International Journal of Ecotoxicology and Ecobiology (Volume 2, Issue 3) |
| DOI | 10.11648/j.ijee.20170203.14 |
| Page(s) | 119-124 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2024. Published by Science Publishing Group |
Streptococcus species, Oreochromis niloticus, SEM, TEM, EDAX
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APA Style
Hosam Saleh, Fatma A. S. Mohamed, Mohamed Abd Al-Razik, Nader Mahmoud, Abeer H. Younis, et al. (2017). Electron Microscopy (SEM-EDAX & TEM) Studies on Streptococcosis in Egyptian Nile Tilapia (Oreochromis niloticus). International Journal of Ecotoxicology and Ecobiology, 2(3), 119-124. https://doi.org/10.11648/j.ijee.20170203.14
ACS Style
Hosam Saleh; Fatma A. S. Mohamed; Mohamed Abd Al-Razik; Nader Mahmoud; Abeer H. Younis, et al. Electron Microscopy (SEM-EDAX & TEM) Studies on Streptococcosis in Egyptian Nile Tilapia (Oreochromis niloticus). Int. J. Ecotoxicol. Ecobiol. 2017, 2(3), 119-124. doi: 10.11648/j.ijee.20170203.14
AMA Style
Hosam Saleh, Fatma A. S. Mohamed, Mohamed Abd Al-Razik, Nader Mahmoud, Abeer H. Younis, et al. Electron Microscopy (SEM-EDAX & TEM) Studies on Streptococcosis in Egyptian Nile Tilapia (Oreochromis niloticus). Int J Ecotoxicol Ecobiol. 2017;2(3):119-124. doi: 10.11648/j.ijee.20170203.14
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author = {Hosam Saleh and Fatma A. S. Mohamed and Mohamed Abd Al-Razik and Nader Mahmoud and Abeer H. Younis and Madiha Salah Ibrahim},
title = {Electron Microscopy (SEM-EDAX & TEM) Studies on Streptococcosis in Egyptian Nile Tilapia (Oreochromis niloticus)},
journal = {International Journal of Ecotoxicology and Ecobiology},
volume = {2},
number = {3},
pages = {119-124},
doi = {10.11648/j.ijee.20170203.14},
url = {https://doi.org/10.11648/j.ijee.20170203.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijee.20170203.14},
abstract = {A field study on Streptococcus infection as a major cause for epidemic infections in Nile Tilapia aquaculture in Kafr El-Shikh Governorate was conducted. This study was carried out to study the gross lesions and post mortem finding of Streptococcsis in Nile Tilapia. Streptococcus was isolated, then identified by Electron Microscopy (Scanning Electron Microscope (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscope (TEM)) to investigate the phenotypic characteristics, ultra-structure of the bacterial cells. Clinical signs of the infected fish showed presence of hemorrhagic patches on different external body regions, ascitis and exophthalmia. Post mortem examinations revealed presence of inflammation of the liver, spleen and kidney. Microbial identification by Gram stain showed Gram positive cocci in shape arranged in chains and the biochemical tests displayed negative catalase and oxidase tests. Electron Microscopy (SEM, EDAX & TEM) confirmed these results and showed the clear cocci shape of streptococcus species and the arrangement of the chains with a bacterial average length of 700 nm. The EDAX results revealed that the elemental structure of the bacterial sample were C,N,O with the concentration 46.93%, 16.61% and 36.46%, respectively.},
year = {2017}
}
TY - JOUR T1 - Electron Microscopy (SEM-EDAX & TEM) Studies on Streptococcosis in Egyptian Nile Tilapia (Oreochromis niloticus) AU - Hosam Saleh AU - Fatma A. S. Mohamed AU - Mohamed Abd Al-Razik AU - Nader Mahmoud AU - Abeer H. Younis AU - Madiha Salah Ibrahim Y1 - 2017/07/25 PY - 2017 N1 - https://doi.org/10.11648/j.ijee.20170203.14 DO - 10.11648/j.ijee.20170203.14 T2 - International Journal of Ecotoxicology and Ecobiology JF - International Journal of Ecotoxicology and Ecobiology JO - International Journal of Ecotoxicology and Ecobiology SP - 119 EP - 124 PB - Science Publishing Group SN - 2575-1735 UR - https://doi.org/10.11648/j.ijee.20170203.14 AB - A field study on Streptococcus infection as a major cause for epidemic infections in Nile Tilapia aquaculture in Kafr El-Shikh Governorate was conducted. This study was carried out to study the gross lesions and post mortem finding of Streptococcsis in Nile Tilapia. Streptococcus was isolated, then identified by Electron Microscopy (Scanning Electron Microscope (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscope (TEM)) to investigate the phenotypic characteristics, ultra-structure of the bacterial cells. Clinical signs of the infected fish showed presence of hemorrhagic patches on different external body regions, ascitis and exophthalmia. Post mortem examinations revealed presence of inflammation of the liver, spleen and kidney. Microbial identification by Gram stain showed Gram positive cocci in shape arranged in chains and the biochemical tests displayed negative catalase and oxidase tests. Electron Microscopy (SEM, EDAX & TEM) confirmed these results and showed the clear cocci shape of streptococcus species and the arrangement of the chains with a bacterial average length of 700 nm. The EDAX results revealed that the elemental structure of the bacterial sample were C,N,O with the concentration 46.93%, 16.61% and 36.46%, respectively. VL - 2 IS - 3 ER -
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