Physical and Chemical Factors of Breeding Sites Affecting Susceptibility and Biochemical Activity of Mosquito, Culex pipiens (Diptera: Culicidae) to Some Insecticides
International Journal of Ecotoxicology and Ecobiology
Volume 4, Issue 2, June 2019, Pages: 51-57
Received: Mar. 23, 2019; Accepted: May 6, 2019; Published: Jun. 4, 2019
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Abdelbaset B. Zayed, Zoology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt
Walaa A. Moselhy, Zoology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt
Azza A. Mostafa, Pesticides Department, Research Institute of Medical Entomology, Ministry of Health & Populations, Giza, Egypt
Hanaa I. Mahmoud, Zoology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt
Shaimaa H. Hassan, Zoology Department, Faculty of Science, Al-Azhar University (Girls Branch), Cairo, Egypt
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Culex pipiens is the common mosquito in Egypt and worldwide species. There is limited evidence on the effects of breeding place nature on the susceptibility of Cx. pipiens to different insecticides. This study aims to evaluate the effects of some physiochemical parameters of different breeding sites in Egypt on Cx. pipiens susceptibility to some recommended insecticides. Mosquito larvae were collected from fresh and sewage water from the selected sites at different areas in Egypt. Water was analyzed in the laboratory to determine salinity and pH. Bioassays and biochemical assays were carried out to determine susceptibility of both larvae and adult under different physiochemical parameters. Larvae and adult that were collected from sewage water with high salinity and acidic medium showed higher resistant to all tested insecticides than those of fresh water with low salinity. The present study provided information of the susceptibility of Cx. pipiens mosquito larvae and adult to some insecticides commonly used under different environmental changes.
Culex pipiens, Insecticide Resistance Assays, Breeding Places, Salinity, pH
To cite this article
Abdelbaset B. Zayed, Walaa A. Moselhy, Azza A. Mostafa, Hanaa I. Mahmoud, Shaimaa H. Hassan, Physical and Chemical Factors of Breeding Sites Affecting Susceptibility and Biochemical Activity of Mosquito, Culex pipiens (Diptera: Culicidae) to Some Insecticides, International Journal of Ecotoxicology and Ecobiology. Vol. 4, No. 2, 2019, pp. 51-57. doi: 10.11648/j.ijee.20190402.12
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Southgate, B. A. (1979): Bancroftian filariasis in Egypt. Tropical diseases bulletin, 76 (12): 1045–1068.
Hanafi, H. A.; Fryauff, D. J.; Saad, M. D.; Soliman, A. K.; Mohareb, E. W.; Medhat, I.; Zayed, A. B.; Szumlas, D. E. and Earhart, K. C. (2011): Virus isolations and high population density implicate Culex antennatus (Becker) (Diptera: Culicidae) as a vector of Rift Valley fever virus during an outbreak in the Nile Delta of Egypt. Acta Trop., 119 (2): 119–124.
Kabula, B. I.; Attah, P. K.; Wilson, M. D. and Boakye, D. A. (2011): Characterization of Anopheles gambiae sl and insecticide resistance profile relative to physiochemical properties of breeding habitats within Accra Metropolis, Ghana. Tanzania Journal of Health Research, 13 (3): 163-187.
Garba, Y. and Olayemi, I. K. (2015): Spartial variation in physiochemical characteristics of wetland rice fields mosquito larval habitats in Minna, north Central Nigeria. In International conference on agricultural, ecological and Medical sciences. (pp. 11-4).
Oyewole, I. O.; Momoh, O. O.; Anyasor, G. N.; Ogunnowo, A. A.; Ibidapo, C. A.; Oduola, O. A. and Awolola, T. S. (2009): Physiochemical characteristics of Anopheles breeding sites: Impact on fecundity and progeny development. African Journal of Environmental Science and Technology, 3 (12).
Harbach RE. (1988): The mosquitoes of the subgenus Culex in southwestern Asia and Egypt (Diptera: Culicidae). Contrib. Amer. Ent. Inst., 24: 1–237.
Chapman, H. C. and Barr, A. R. (1969): Techniques for successful colonization of many mosquito species. Mosquito, News, 29: 532-535.
World Health Organization (2006): Pesticides and their applications, for the control of vectors and pests of public health importance. WHO/ CDS/ NTD/ WHOPES/ GCDPP/1.
World Health Organization. (1981): Instructions for determining the susceptibility or resistance of mosquito larvae to insecticides. WHO/ VBC/ 81.807.
World Health Organization (2005): Guidelines for laboratory and field testing of mosquito larvicides.
World Health Organization. (1998): Test procedures for insecticide resistance monitoring in malaria vectors, bio-efficacy and persistence of insecticides on treated surfaces: report of the WHO informal consultation, Geneva, 28-30 September 1998.
Amin, T. R. (1998): Biochemical and physiological studies of some insect growth regulators on the cotton leafworm, Spodoptera littoralis (Boisd.) (Doctoral dissertation, Ph. D. thesis, Faculty of science, Cairo Univ).‏
Bradford, M. M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72 (1-2): 248-254.
Simpson, D. R.; Bulland, D. L. and Linquist, D. A. (1964): A semimicro technique for estimation of cholinesterase activity in boll weevils. Entomological Society of American, 57: 367- 371.
Habig, W. H.; Pabst, M. J. and Jakoby, W. B. (1974): Glutathione S-transferases the first enzymatic step in mercapturic acid formation. Journal of biological Chemistry, 249 (22), 7130-7139.
Barrett, K. C.; Morgan, G. A.; Leech, N. L. and Gloeckner, G. W. (2012): IBM SPSS for introductory statistics: Use and interpretation. Routledge.
World Health Organization (2013): Test procedures for insecticide resistance monitoring in malaria vector mosquitoes.
Gould, E. A. and Higgs, S. (2009): Impact of climate change and other factors on emerging arbovirus diseases. Transactions of the Royal Society of Tropical Medicine and Hygiene, 103 (2), 109-121.‏
Kipyab, P. C.; Khaemba, B. M.; Mwangangi, J. M. and Mbogo, C. M. (2015): The physiochemical and environmental factors affecting the distribution of Anopheles merus along the Kenyan coast. Parasites & vectors, 8 (1): 221.
Banerjee, S.; Mohan, S.; Saha, N.; Mohanty, S. P.; Saha, G. K. and Aditya, G. (2015): Pupal productivity & nutrient reserves of Aedes mosquitoes breeding in sewage drains & other habitats of Kolkata, India: implications for habitat expansion & vector management. The Indian journal of medical research, 142 (Suppl 1): S87.
Juliano, S. A.; Lounibos, L. P. and O’Meara, G. F. (2004): A field test for competitive effects of Aedes albopictus on A. aegypti in South Florida: differences between sites of coexistence and exclusion? Oecologia, 139 (4): 583-593.
Murrell, E. G. and Juliano, S. A. (2008): Detritus type alters the outcome of interspecific competition between Aedes aegypti and Aedes albopictus (Diptera: Culicidae). Journal of Medical Entomology, 45 (3): 375-383.
Kady, G. A. E. L.; Kamel, N. H.; Mosleh, Y. Y. and Bahght, I. M. (2008): Comparative toxicity of two bio-insecticides (Spinotoram and Vertemic) compared with methomyl against Culex pipiens and Anopheles multicolor. World Journal of Agricultural Sciences, 4 (2): 198-205.
Yang, M. L.; Zhang, J. Z.; Zhu, K. Y.; Xuan, T.; Liu, X. J.; Guo, Y. P. and Ma, E. B. (2009): Mechanisms of organophosphate resistance in a field population of oriental migratory locust, Locusta migratoria manilensis (Meyen). Archives of Insect Biochemistry and Physiology: Published in Collaboration with the Entomological Society of America, 71 (1): 3-15.‏
Clark, T. M.; Flis, B. J. and Remold, S. K. (2004): Differences in the effects of salinity on larval growth and developmental programs of a freshwater and a euryhaline mosquito species (Insecta: Diptera, Culicidae). Journal of Experimental Biology, 207 (13): 2289-2295.
Owusu, H. F.; Chitnis, N. and Müller, P. (2017): Insecticide susceptibility of Anopheles mosquitoes changes in response to variations in the larval environment. Scientific reports, 7 (1): 3667.
Jude, P. J.; Tharmasegaram, T.; Sivasubramaniyam, G.; Senthilnanthanan, M.; Kannathasan, S.; Raveendran, S. and Surendran, S. N. (2012): Salinity-tolerant larvae of mosquito vectors in the tropical coast of Jaffna, Sri Lanka and the effect of salinity on the toxicity of Bacillus thuringiensis to Aedes aegypti larvae. Parasites & vectors, 5 (1): 269.
Osborn, F. R.; Herrera, M. J.; Gómez, C. J. and Salazar, A. (2007): Comparison of two commercial formulations of Bacillus thuringiensis var. israelensis for the control of Anopheles aquasalis (Diptera: Culicidae) at three salt concentrations. Memórias do Instituto Oswaldo Cruz., 102 (1): 69-72.
Kulma, K.; Saddler, A. and Koella, J. C. (2013): Effects of age and larval nutrition on phenotypic expression of insecticide-resistance in Anopheles mosquitoes. PLoS One, 8 (3): e58322.
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