A Systematic Comparison of Biosurfactant Effects on Physicochemical Properties and Growth Rates of <i>P. aeruginosa</i> MM1011 and TMU56: A Bioremediation Perspective

Fatemeh Tazari; Mahdi Rahaie; Ashrafosadat Hatamian Zarmi; Fatemeh Yazdian; Hassan Jalili; Salman Alizadeh Kordkandi

doi:doi:10.11648/j.be.20170101.13

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A Systematic Comparison of Biosurfactant Effects on Physicochemical Properties and Growth Rates of P. aeruginosa MM1011 and TMU56: A Bioremediation Perspective

Fatemeh Tazari, Mahdi Rahaie, Ashrafosadat Hatamian Zarmi, Fatemeh Yazdian, Hassan Jalili, Salman Alizadeh Kordkandi

Published in Bioprocess Engineering (Volume 1, Issue 1)

Received: 16 March 2017 Accepted: 12 April 2017 Published: 8 June 2017

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Abstract

The main objective of this research was to focus on enhancing the substrate uptake rate of P. aeruginosa using various biosurfactants as well as carbon sources in the medium culture. While hexadecane and Polychlorinated Biphenyls (PCBs) were chosen as hydrophobic carbon sources, the effects of glucose on two strains of P. aeruginosa, MM1011 and TMU56, were evaluated. Two kinds of biosurfactants, including surfactin and rhamnolipid at higher and lower than the critical micelle concentrations were added into the medium. After that, the response of bacterium based on cell surface hydrophobicity (CSH) was measured through the BATH assay. General full factorial technique was used to organize the experiments and analyze the effects of input factors on CSH. Although the both strains showed similar growth trend under conditions of different carbon sources, the order of affinity between the various substrates and the specific growth rates was as PCBs> glucose> nutrient broth> hexadecane. The analysis of variance showed that both type of carbon source and the biosurfactant had a significant effect on the CSH of P. aeruginosa TMU56. However, the P. aeruginosa MM1011 strain had no meaningful reaction in the presence of biosurfactant. High value of coefficient of determination (R²=0.95) indicated a good agreement between experimental data and predicted values by models. Moreover, SDS-PAGE analysis demonstrated that the variation in hydrophobicity was a result of fluctuation in the amount of major proteins on the bacteria cell wall. The significant effect of biosurfactant on the P. aeruginosa TMU56 at concentration under critical micelle point was related to the release of more outer membrane proteins (OMPs).

Published in	Bioprocess Engineering (Volume 1, Issue 1)
DOI	10.11648/j.be.20170101.13
Page(s)	14-20
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Biosurfactant, Cell Surface Hydrophobicity, Factorial Design, Statistical Analysis

References

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Fatemeh Tazari, Mahdi Rahaie, Ashrafosadat Hatamian Zarmi, Fatemeh Yazdian, Hassan Jalili, et al. (2017). A Systematic Comparison of Biosurfactant Effects on Physicochemical Properties and Growth Rates of P. aeruginosa MM1011 and TMU56: A Bioremediation Perspective. Bioprocess Engineering, 1(1), 14-20. https://doi.org/10.11648/j.be.20170101.13

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ACS Style

Fatemeh Tazari; Mahdi Rahaie; Ashrafosadat Hatamian Zarmi; Fatemeh Yazdian; Hassan Jalili, et al. A Systematic Comparison of Biosurfactant Effects on Physicochemical Properties and Growth Rates of P. aeruginosa MM1011 and TMU56: A Bioremediation Perspective. Bioprocess Eng. 2017, 1(1), 14-20. doi: 10.11648/j.be.20170101.13

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AMA Style

Fatemeh Tazari, Mahdi Rahaie, Ashrafosadat Hatamian Zarmi, Fatemeh Yazdian, Hassan Jalili, et al. A Systematic Comparison of Biosurfactant Effects on Physicochemical Properties and Growth Rates of P. aeruginosa MM1011 and TMU56: A Bioremediation Perspective. Bioprocess Eng. 2017;1(1):14-20. doi: 10.11648/j.be.20170101.13

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@article{10.11648/j.be.20170101.13,
  author = {Fatemeh Tazari and Mahdi Rahaie and Ashrafosadat Hatamian Zarmi and Fatemeh Yazdian and Hassan Jalili and Salman Alizadeh Kordkandi},
  title = {A Systematic Comparison of Biosurfactant Effects on Physicochemical Properties and Growth Rates of P. aeruginosa MM1011 and TMU56: A Bioremediation Perspective},
  journal = {Bioprocess Engineering},
  volume = {1},
  number = {1},
  pages = {14-20},
  doi = {10.11648/j.be.20170101.13},
  url = {https://doi.org/10.11648/j.be.20170101.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.be.20170101.13},
  abstract = {The main objective of this research was to focus on enhancing the substrate uptake rate of P. aeruginosa using various biosurfactants as well as carbon sources in the medium culture. While hexadecane and Polychlorinated Biphenyls (PCBs) were chosen as hydrophobic carbon sources, the effects of glucose on two strains of P. aeruginosa, MM1011 and TMU56, were evaluated. Two kinds of biosurfactants, including surfactin and rhamnolipid at higher and lower than the critical micelle concentrations were added into the medium. After that, the response of bacterium based on cell surface hydrophobicity (CSH) was measured through the BATH assay. General full factorial technique was used to organize the experiments and analyze the effects of input factors on CSH. Although the both strains showed similar growth trend under conditions of different carbon sources, the order of affinity between the various substrates and the specific growth rates was as PCBs> glucose> nutrient broth> hexadecane. The analysis of variance showed that both type of carbon source and the biosurfactant had a significant effect on the CSH of P. aeruginosa TMU56. However, the P. aeruginosa MM1011 strain had no meaningful reaction in the presence of biosurfactant. High value of coefficient of determination (R2=0.95) indicated a good agreement between experimental data and predicted values by models. Moreover, SDS-PAGE analysis demonstrated that the variation in hydrophobicity was a result of fluctuation in the amount of major proteins on the bacteria cell wall. The significant effect of biosurfactant on the P. aeruginosa TMU56 at concentration under critical micelle point was related to the release of more outer membrane proteins (OMPs).},
 year = {2017}
}

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TY - JOUR
T1 - A Systematic Comparison of Biosurfactant Effects on Physicochemical Properties and Growth Rates of P. aeruginosa MM1011 and TMU56: A Bioremediation Perspective
AU - Fatemeh Tazari
AU - Mahdi Rahaie
AU - Ashrafosadat Hatamian Zarmi
AU - Fatemeh Yazdian
AU - Hassan Jalili
AU - Salman Alizadeh Kordkandi
Y1 - 2017/06/08
PY - 2017
N1 - https://doi.org/10.11648/j.be.20170101.13
DO - 10.11648/j.be.20170101.13
T2 - Bioprocess Engineering
JF - Bioprocess Engineering
JO - Bioprocess Engineering
SP - 14
EP - 20
PB - Science Publishing Group
SN - 2578-8701
UR - https://doi.org/10.11648/j.be.20170101.13
AB - The main objective of this research was to focus on enhancing the substrate uptake rate of P. aeruginosa using various biosurfactants as well as carbon sources in the medium culture. While hexadecane and Polychlorinated Biphenyls (PCBs) were chosen as hydrophobic carbon sources, the effects of glucose on two strains of P. aeruginosa, MM1011 and TMU56, were evaluated. Two kinds of biosurfactants, including surfactin and rhamnolipid at higher and lower than the critical micelle concentrations were added into the medium. After that, the response of bacterium based on cell surface hydrophobicity (CSH) was measured through the BATH assay. General full factorial technique was used to organize the experiments and analyze the effects of input factors on CSH. Although the both strains showed similar growth trend under conditions of different carbon sources, the order of affinity between the various substrates and the specific growth rates was as PCBs> glucose> nutrient broth> hexadecane. The analysis of variance showed that both type of carbon source and the biosurfactant had a significant effect on the CSH of P. aeruginosa TMU56. However, the P. aeruginosa MM1011 strain had no meaningful reaction in the presence of biosurfactant. High value of coefficient of determination (R2=0.95) indicated a good agreement between experimental data and predicted values by models. Moreover, SDS-PAGE analysis demonstrated that the variation in hydrophobicity was a result of fluctuation in the amount of major proteins on the bacteria cell wall. The significant effect of biosurfactant on the P. aeruginosa TMU56 at concentration under critical micelle point was related to the release of more outer membrane proteins (OMPs).
VL - 1
IS - 1
ER -

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Author Information

Fatemeh Tazari

Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
Mahdi Rahaie

Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
Ashrafosadat Hatamian Zarmi

Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
Fatemeh Yazdian

Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
Hassan Jalili

Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
Salman Alizadeh Kordkandi

Environmental Engineering Research Center (EERC), Sahand University of Technology, Tabriz, Iran

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APA Style

Fatemeh Tazari, Mahdi Rahaie, Ashrafosadat Hatamian Zarmi, Fatemeh Yazdian, Hassan Jalili, et al. (2017). A Systematic Comparison of Biosurfactant Effects on Physicochemical Properties and Growth Rates of P. aeruginosa MM1011 and TMU56: A Bioremediation Perspective. Bioprocess Engineering, 1(1), 14-20. https://doi.org/10.11648/j.be.20170101.13

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Fatemeh Tazari; Mahdi Rahaie; Ashrafosadat Hatamian Zarmi; Fatemeh Yazdian; Hassan Jalili, et al. A Systematic Comparison of Biosurfactant Effects on Physicochemical Properties and Growth Rates of P. aeruginosa MM1011 and TMU56: A Bioremediation Perspective. Bioprocess Eng. 2017, 1(1), 14-20. doi: 10.11648/j.be.20170101.13

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AMA Style

Fatemeh Tazari, Mahdi Rahaie, Ashrafosadat Hatamian Zarmi, Fatemeh Yazdian, Hassan Jalili, et al. A Systematic Comparison of Biosurfactant Effects on Physicochemical Properties and Growth Rates of P. aeruginosa MM1011 and TMU56: A Bioremediation Perspective. Bioprocess Eng. 2017;1(1):14-20. doi: 10.11648/j.be.20170101.13

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@article{10.11648/j.be.20170101.13,
  author = {Fatemeh Tazari and Mahdi Rahaie and Ashrafosadat Hatamian Zarmi and Fatemeh Yazdian and Hassan Jalili and Salman Alizadeh Kordkandi},
  title = {A Systematic Comparison of Biosurfactant Effects on Physicochemical Properties and Growth Rates of P. aeruginosa MM1011 and TMU56: A Bioremediation Perspective},
  journal = {Bioprocess Engineering},
  volume = {1},
  number = {1},
  pages = {14-20},
  doi = {10.11648/j.be.20170101.13},
  url = {https://doi.org/10.11648/j.be.20170101.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.be.20170101.13},
  abstract = {The main objective of this research was to focus on enhancing the substrate uptake rate of P. aeruginosa using various biosurfactants as well as carbon sources in the medium culture. While hexadecane and Polychlorinated Biphenyls (PCBs) were chosen as hydrophobic carbon sources, the effects of glucose on two strains of P. aeruginosa, MM1011 and TMU56, were evaluated. Two kinds of biosurfactants, including surfactin and rhamnolipid at higher and lower than the critical micelle concentrations were added into the medium. After that, the response of bacterium based on cell surface hydrophobicity (CSH) was measured through the BATH assay. General full factorial technique was used to organize the experiments and analyze the effects of input factors on CSH. Although the both strains showed similar growth trend under conditions of different carbon sources, the order of affinity between the various substrates and the specific growth rates was as PCBs> glucose> nutrient broth> hexadecane. The analysis of variance showed that both type of carbon source and the biosurfactant had a significant effect on the CSH of P. aeruginosa TMU56. However, the P. aeruginosa MM1011 strain had no meaningful reaction in the presence of biosurfactant. High value of coefficient of determination (R2=0.95) indicated a good agreement between experimental data and predicted values by models. Moreover, SDS-PAGE analysis demonstrated that the variation in hydrophobicity was a result of fluctuation in the amount of major proteins on the bacteria cell wall. The significant effect of biosurfactant on the P. aeruginosa TMU56 at concentration under critical micelle point was related to the release of more outer membrane proteins (OMPs).},
 year = {2017}
}

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