Biophysical-chemistry Characterization of Alginate in Brown Algae Species Sargassum dupplicatum
World Journal of Food Science and Technology
Volume 4, Issue 1, March 2020, Pages: 17-22
Received: Mar. 17, 2020; Accepted: Apr. 2, 2020; Published: Apr. 29, 2020
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Dang Xuan Cuong, Organic Matterial from Marine Resource, Nhatrang Institute of Technology Application and Research, Vietnam Academic of Science and Technology, Nha Trang, Vietnam
Dang Thi Thanh Tuyen, Faculty of Food Technology, Nha Trang University, Nha Trang, Vietnam
Do Thuy Kieu, Faculty of Food Technology, Nha Trang University, Nha Trang, Vietnam
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Alginate is a high-value biopolymer, exists in brown algae, and applied widely in numerous fields, for example, food, functional foods, and pharmaceutics. The study focused on the biophysical-chemistry characterization of alginate in brown algae species Sargassum dupplicatum grown commonly in Vietnam under the effect of NaCl, KCl, MgCl2, chitosan, carrageenan, and ethanol in different physical condition. Antioxidant activity of alginate and their compound was also studied. The results showed that Na_Alg, K_Alg, and Mg_Alg disperse the net style in the water. Ca_Alg, chitosan_Alg, and Carrageenan_Alg absorbed water and swelled for forming a sphere, yarn, and yarn in the water, respectively. Chitosan_Alg and carrageenan_Alg precipitated faster in ethanol, compared to Na_Alg, K_Alg, and Mg_Alg. 20% of ethanol did not cause the precipitation of Alginate salt, Chitosan_Alg, and carrageenan_Alg for 30 minutes. Alginate salt, Chitosan_Alg, and Carrageenan_Alg were full precipitated for 30 minutes when using ethanol concentration was more than 80%. Chitosan_Alg and Carrageenan_Alg occurred the precipitation in 20% of ethanol for 30 minutes. Total antioxidant activity and reducing power activity of chitosan_Alg got the highest value (a and b, respectively), compared to Carrageenan_Alg, Na_Alg, K_Alg, and Mg_Alg. The antioxidant activity difference in a group of Na_Alg, K_Alg, and Mg_Alg, and a group (Chitosan_Alg and Carrageenan_Alg) did not happen. The difference only occurred between the two groups.
Alginate, Chitosan, Carrageenan, Antioxidant, Ethanol
To cite this article
Dang Xuan Cuong, Dang Thi Thanh Tuyen, Do Thuy Kieu, Biophysical-chemistry Characterization of Alginate in Brown Algae Species Sargassum dupplicatum, World Journal of Food Science and Technology. Special Issue: Marine Bio-Polymer: Bio-Activity, Extraction and Application. Vol. 4, No. 1, 2020, pp. 17-22. doi: 10.11648/j.wjfst.20200401.13
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Sellimi S., et al. (2015). Structural, physicochemical and antioxidant properties of sodium alginate isolated from a Tunisian brown seaweed. Int J Biol Macromol, 72 1358-1367.
Zaneta K., Krzysztof, M., Dominika, K., Monika, M. and Andrzej, J. (2017). Cytotoxicity, bactericidal, and antioxidant activity of sodium alginate hydrosols treated with direct electric current. Int J Mol Sci, 18 678-697.
Milda E. and Kerry, C., Edible films and coatings for food applications, Springer Science+Business Media, 2009, pp. 71.
Martin A. and Cristian, O. (2014). Review of the characterization of sodium alginate by intrinsic viscosity measurements. Comparative analysis between conventional and single point methods. International Journal of BioMaterials Science and Engineering, 1 (1): 1-11.
Magdalena B. and Grzegorz, S. (2015). The effect of sodium alginate concentration on the rheological parameters of spinning solutions. AUTEX Research Journal, 15 (2): 123-126.
Kuen Y. and David, J. (2012). Alginate: properties and biomedical applications. Prog Polym Sci, 37 (1): 106-126.
Kelishomi Z., et al. (2016). Antioxidant activity of low molecular weight alginate produced by thermal treatment. Food Chem, 196 897-902.
Mandel K., Daggy, B., Brodie, D. and Jacoby, H. (2000). Review article: alginate-raft formulations in the treatment of heartburn and acid reflux. Aliment Pharmacol Ther, 14 669-690.
Leiman D., et al. (2017). Alginate therapy is effective treatment for GERD symptoms: a systematic review and meta-analysis. Dis Esophagus, 30 1-9.
Hee M. (2016). Raft formation of sodium alginate in the stomach. J Neurogastroenterol Motil, 22 (4): 705-706.
Atsuki Y., Tomokazu, I., Reishi, N. and Ryuichi, N. (2014). Sodium alginate ameliorates indomethacin-induced gastrointestinal mucosal injury via inhibiting translocation in rats. World J Gastroenterol, 20 (10): 641-2652.
Aluani D., et al. (2017). Еvaluation of biocompatibility and antioxidant efficiency of chitosan-alginate nanoparticles loaded with quercetin. Int J Biol Macromol, 103 771-782.
Ahmed M., Shereen, N., Mohie, M. and Mohamed, E. (2016). Effect of microencapsulation on chemical composition and antioxidant activity of cumin and fennel essential oils. Res J Pharm Biol Chem Sci, 7 (3): 1565-1574.
Ana B., et al. (2011). Encapsulation of polyphenolic antioxidants from medicinal plant extracts in alginate-chitosan system enhanced with ascorbic acid by electrostatic extrusion. Food Res Int, 44 1094-1101.
Geetha T., Deol, P. and Kaur, I. (2015). Role of sesamol-loaded floating beads in gastric cancers: a pharmacokinetic and biochemical evidence. J Microencapsul, 32 (5): 478-487.
Pilar J., Elena, P., Teresa, S., Javier, O., Fernando, S. and Angel, L. (2005). Free radicals and antioxidant systems in reflux esophagitis and Barrett’s esophagus. World J Gastroenterol, 11 (18): 2697-2703.
Wetscher GJ, Hinder, R., Klingler, P., Gadenstatter, M., Perdikis, G. and Hinder, P. (1997). Reflux esophagitis in humans is a free radical event. Diseases of the Esophagus, 10 29-32.
Norimasa Y. (2007). Inflammation and oxidative stress in gastroesophageal reflux disease. J Clin Biochem Nutr, 40 13-23.
Michael A., Alan, T., Stefan, K., Akira, P., Kjersti, S. and Masahiro, N. (2009). Nineteenth International Seaweed Symposium. J Appl Phycol, 20 (5): 571-577.
Mattio L., Payri, C. and Verlaque, M. (2009). Taxonomic revision and geographic distribution of the subgenus Sargassum (Fucales, Phaeophyceae) in the western and central Pacific islands based on morphological and molecular analyses. J Phycol, 45 (5): 1213-1227.
Ervia Y. and Alim, I. (2016). Characterizing the three different alginate type of Sargassum siliquosum. IJMS, 22 (1): 7-14.
Decky J. I. and Emil, B. (2013). A study of extraction and characterization of alginates obtained from brown macroalgae Sargassum duplicatum and Sargassum crassifolium from Indonesia. 46 (2): 65-70.
Trang S. T. and Pham, T. D. P. (2012). Bioactive compounds from by-products of shrimp processing industry in Vietnam. J Food Drug Anal, 20 (1): 194-197.
Richardson J., Dettmar, P., Hampson, F. and Melia, C. (2004). A simple, high throughput method for the quantification of sodium alginates on oesophageal mucosa. Eur J Pharm Biopharm, 57 299–305.
Prieto P., Pineda, M. and Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem, 269 (2): 337-341.
Zhu Q., Hackman, R., Ensunsa, J., Holt, R. and Keen, C. (2002). Antioxidative activities of Oolong tea. J Agric Food Chem, 50 (23): 6929-6934.
A P., Sh, B. and GR, M. (2006). MBA-crosslinked Na-Alg/CMC as a smart full-polysaccharide superabsorbent hydrogels. Carbohydrate Polymers, 66 (3, 2): 386-395.
Daemi H. and Barikani, M. (2012). Synthesis and characterization of calcium alginate nanoparticles, sodium homopolymannuronate salt and its calcium nanoparticles. Scientia Iranica F, 19 (6): 2023–2028.
Vijayaraghavana G. and Shanthakumar, S. (2016). Performance study on algal alginate as natural coagulant for the removal of Congo red dye. Desalin Water Treat, 57 (14): 6384-6392.
Williams D. and Fleming I, Spectroscopic methods in organic chemistry, 5th, London, 1997, pp. 27-57.
Huisuo H., Ingolf, U. G., Mark, E. and Andrew, D. C. (2017). Measurement of total sodium alginate in restructured fish products using fourier transform infrared spectroscopy. EC Nutrition, 11 (1): 33-45.
Shuting Z. and Jianlong, W. (2018). Modified alginate beads as biosensor and biosorbent for simultaneous detection and removal of cobalt ions from aqueous solution. Environ Prog Sustain, 37 (1): 260-266.
Samira N.-S., Hajar, S. and Mahdi, K. (2016). J Biomater Sci Polym Ed. Encapsulation optimization of lemon balm antioxidants in calcium alginate hydrogels, 27 (16): 1-29.
Alex L. C., Lorena, D. and Miriam Martino (2013). Effect of starch filler on calcium-alginate hydrogels loaded with yerba mate antioxidants. Carbohydr Polym, 95 (1, 5): 315-323.
Yuan H., et al. (2005). Preparation and in vitro antioxidant activity of kappa-carrageenan oligosaccharides and their oversulfated, acetylated, and phosphorylated derivatives. Carbohydr Res, 340 (4): 685-692.
Ngo D. and Kim, S. (2014). Antioxidant effects of chitin, chitosan, and their derivatives. Adv Food Nutr Res, 73 15-31.
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