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Invitro Treatment of Blue Ticks Using a Phytosubstance

Received: 4 June 2019    Accepted: 4 July 2019    Published: 25 November 2019
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Abstract

Ticks are some of the most devastating ectoparasites of livestock causing several tick borne diseases. Management of ticks is increasing getting difficulty using synthetic acaricides due their rapid tolerance to the synthetic acaricides. Botanical acaricides are now used as an alternative management strategy to curb such problems. The blue tick, Rhiphicephalus (Boophilus) decolaratus, is a one-host tick that parasitizes cattle and vectors a debilitating protozoan parasite that causes babesiosis. Acaricidal properties of thyme were investigated on the cattle blue tick, R.B. decolaratus using various concentrations to establish the lethal dose concentration. The acaridal activity of thyme on ticks was monitored and recorded at two-hour intervals for 48 hours. Tick mortality was observed at all concentrations except the control. The lowest mortality rate was observed for 10μl/cm3 while 40μl/cm3 resulted in total tick mortality. The LC50 was established as 1,9μl/cm3. Significant differences (p<0.01) were observed between concentrations, however the mortality was directly proportional to thyme concentration. The thyme oil dehydrated the ticks leading to death within 48 hours. The study thus concludes that thyme oil can be used as a complimentary or alternative acaricide in an integrated livestock program or where organic means are enforced or to as a way of minimizing tolerance of ticks to conventional acaricides.

Published in American Journal of Entomology (Volume 3, Issue 4)
DOI 10.11648/j.aje.20190304.11
Page(s) 66-69
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Acaricidal, Mortality, Lethal Dose, Thyme Oil, Bioassay

References
[1] Bekalu, G. E. and Angesom, H. D., (2016). Review on the Impact of Ticks on Livestock Health and Productivity. Journal of Biology, Agriculture and Healthcare 6 (22), 1.
[2] Meltzer M. I, Norval, R. A. and Donachie, P. L., (1995). Effects of tick infestation and tick-borne disease infections (heartwater, anaplasmosis and babesiosis) on the lactation and weight gain of Mashona cattle in southeastern Zimbabwe. Tropical Animal Health Production 27 (3), 129-44.
[3] Pegram, R. G, James, A. D., Oosterwijk, G. P. and Killorn, K. J., (1991). Studies on the economics of ticks in Zambia. Experimental Applications in Acarology, 12, 9-26.
[4] Piesman, J. and Eisen, L., (2008), Prevention of tick-borne diseases. Annual Reviews of Entomology 53, 323–343.
[5] Smeenk, I., Kelly, P. J., Wray, K., Musuka, G., Trees, A. J. and Jongejan, F., (2000). Babesia bovis and B. bigemina DNA detected in cattle and ticks from Zimbabwe by polymerase chain reaction. Journal of South African Veterinary Association, 71 (1), 21-4.
[6] El-Gengaihi, S. E., Amer, S. A. A. and Mohamed, S. M., (1996). Biological Activity of Thyme Oil and Thymol against Tetranychus urticae Koch. Anzeiger Für Schädlingskunde, Pflanzenschutz, Umweltschutz 69 (7), 157–159.
[7] Norval, R. A., Fivaz, B. H., Lawrence, J. A., Daillecourt. T., (1983). Epidemiology of tick-borne diseases of cattle in Zimbabwe. I. Babesiosis. Tropical Animal Health Production 15 (2), 87-94.
[8] Tak, J. H.; Jovel, E.; Isman, M. B. (2016), Contact, fumigant, and cytotoxic activities of thyme and lemongrass essential oils against larvae and an ovarian cell line of the cabbage looper, Trichoplusia ni. Journal of Pesticide Science 89, 183–193.
[9] Balan Banumathi, Balasubramanian Malaikozhundan, Baskaralingam Vaseeharan, Invitro acaricidal activity of ethnoveterinary plants and greensynthesis of zinc oxide nanoparticles against Rhipicephalus (Boophilus)microplus, Veterinary parasitology DOI: 10.1016/j.vetpar.2015.12.003
[10] Sertkaya, E., Kaya, K., Soylu, S., (2010), Acaricidal activities of the essential oils from several medicinal plants againstthe carmine spider mite (Tetranychus cinnabarinus Boisd.)(Acarina: Tetranychidae). Ind. Crops Prod. 31, 107–112.
[11] Tak, J. H., Jovel, E., Isman, M. B., (2016). Comparative and synergistic activity of Rosmarinus officinalis L. essential oilconstituents against the larvae and an ovarian cell line of the cabbage looper, Trichoplusia ni (Lepidoptera: Noctuidae). Pest Management Science 72, 474–480.
[12] Ma, W. B., Feng, J. T., Jiang, Z. L., Zhang, X., (2014). Fumigant activity of 6 selected essential oil compounds and combined effect of methyl salicylate and trans-cinnamaldehyde against Culex pipiens pallens. Journal of American Mosquito Control, 30, 199–203.
[13] Wu, Y., Zhang, W. J., Huang, D. Y., Wang, Y., Wei, J. Y., Li, Z. H., Sun, J. S., Bai, J. F., Tian, Z. F., Wang, P. J., (2015). Chemical compositions and insecticidal activities of Alpinia kwangsiensis Essential Oil against Lasioderma serricorne. Molecules 20, 21939–21945.
[14] Miresmailli, S., Bradbury, R., Isman, M. B., (2006). Comparative toxicity of Rosmarinus officinalis L. essential oil and blends of its major constituents against Tetranychus urticae Koch (Acari: Tetranychidae) on two different host plants. Pest Management Science, 62, 366–371.
[15] Mansour, F., Azaizeh, H., Saad, B., Tadmor, Y., Abo-Moch, F., Said, O., (2004). The potential of Middle Eastern flora as a source of new safe bio-acaricides to control Tetranychus cinnabarinus, the carmine spider mite. Phytoparasitica 32, 66–72.
[16] Ranger, C. M., Reding, M. E., Oliver, J. B., Moyseenko, J. J., Youssef, N., Krause, C. R., (2013). Acute toxicity of plant essential oils to scarab larvae (Coleoptera: Scarabaeidae) and their analysis by gas chromatography-mass spectrometry. Journal of Economic Entomology 106, 159–167.
[17] Blenau, W. Rademacher, E. Baumann, A., (2012). Plant essential oils and formamidines as insecticides/acaricides: What are the molecular targets? Apidologie 43, 334–347.
[18] Seo, S. M., Park, H. M., Park, I. K., (2012). Larvicidal activity of ajowan (Trachyspermum ammi) and Peru balsam (Myroxylon pereira) oils and blends of their constituents against mosquito, Aedes aegypti, acute toxicity on water flea, Daphnia magna, and aqueous residue. Journal of Agriculture and Food Chemistry 60, 5909–5914.
[19] Tabari, M. A., Youssefi, M. R., Barimani, A., Araghi, A., (2015). Carvacrol as a potent natural acaricide against Dermanyssus gallinae. Parasitology Research 114, 3801–3806.
[20] Opiro, R., Osinde, C, Okello-Onen, J. and Akol, A. M., (2013). Tick-repellent properties of four plant species against Rhipicephalus appendiculatus Neumann (Acarina: Ixodidae) tick species. E3 Journal of Agricultural Research and Development 3 (2), 017-021.
[21] Janadaree Bandara, K. M. U. and Parakrama Karunaratne, S. H. P., (2017). Mechanisms of acaricide resistance in the cattle tick Rhipicephalus (Boophilus) microplus in Sri Lanka. Pesticide Biochemistry and Physiology 139, 68–72
[22] Singh, A., Khare, A. and Singh, A. P., (2012). Use of Vegetable Oils as Biopesticide in Grain Protection-A Review. Journal of Biofertilizers and Biopesticides 3, 114. doi:10.4172/2155-6202.1000114.
[23] Giglioti, R., Forim, M. R., Oliveira, H. N., Chagas, A. C. S., Ferrezini, J., Brito, L. G., Falcoski, T. O. R. S., Albuquerque L. G. and Oliveira, M. C. S. (2011). In vitro acaricidal activity of neem (Azadirachta indica) seed extracts with known azadirachtin concentrations against Rhipicephalus microplus. Veterinary Paraitology 181 (2-4), 309-315.
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  • APA Style

    Nyembezi Mgocheki, Jenias Ndava. (2019). Invitro Treatment of Blue Ticks Using a Phytosubstance. American Journal of Entomology, 3(4), 66-69. https://doi.org/10.11648/j.aje.20190304.11

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    ACS Style

    Nyembezi Mgocheki; Jenias Ndava. Invitro Treatment of Blue Ticks Using a Phytosubstance. Am. J. Entomol. 2019, 3(4), 66-69. doi: 10.11648/j.aje.20190304.11

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    AMA Style

    Nyembezi Mgocheki, Jenias Ndava. Invitro Treatment of Blue Ticks Using a Phytosubstance. Am J Entomol. 2019;3(4):66-69. doi: 10.11648/j.aje.20190304.11

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  • @article{10.11648/j.aje.20190304.11,
      author = {Nyembezi Mgocheki and Jenias Ndava},
      title = {Invitro Treatment of Blue Ticks Using a Phytosubstance},
      journal = {American Journal of Entomology},
      volume = {3},
      number = {4},
      pages = {66-69},
      doi = {10.11648/j.aje.20190304.11},
      url = {https://doi.org/10.11648/j.aje.20190304.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aje.20190304.11},
      abstract = {Ticks are some of the most devastating ectoparasites of livestock causing several tick borne diseases. Management of ticks is increasing getting difficulty using synthetic acaricides due their rapid tolerance to the synthetic acaricides. Botanical acaricides are now used as an alternative management strategy to curb such problems. The blue tick, Rhiphicephalus (Boophilus) decolaratus, is a one-host tick that parasitizes cattle and vectors a debilitating protozoan parasite that causes babesiosis. Acaricidal properties of thyme were investigated on the cattle blue tick, R.B. decolaratus using various concentrations to establish the lethal dose concentration. The acaridal activity of thyme on ticks was monitored and recorded at two-hour intervals for 48 hours. Tick mortality was observed at all concentrations except the control. The lowest mortality rate was observed for 10μl/cm3 while 40μl/cm3 resulted in total tick mortality. The LC50 was established as 1,9μl/cm3. Significant differences (p<0.01) were observed between concentrations, however the mortality was directly proportional to thyme concentration. The thyme oil dehydrated the ticks leading to death within 48 hours. The study thus concludes that thyme oil can be used as a complimentary or alternative acaricide in an integrated livestock program or where organic means are enforced or to as a way of minimizing tolerance of ticks to conventional acaricides.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Invitro Treatment of Blue Ticks Using a Phytosubstance
    AU  - Nyembezi Mgocheki
    AU  - Jenias Ndava
    Y1  - 2019/11/25
    PY  - 2019
    N1  - https://doi.org/10.11648/j.aje.20190304.11
    DO  - 10.11648/j.aje.20190304.11
    T2  - American Journal of Entomology
    JF  - American Journal of Entomology
    JO  - American Journal of Entomology
    SP  - 66
    EP  - 69
    PB  - Science Publishing Group
    SN  - 2640-0537
    UR  - https://doi.org/10.11648/j.aje.20190304.11
    AB  - Ticks are some of the most devastating ectoparasites of livestock causing several tick borne diseases. Management of ticks is increasing getting difficulty using synthetic acaricides due their rapid tolerance to the synthetic acaricides. Botanical acaricides are now used as an alternative management strategy to curb such problems. The blue tick, Rhiphicephalus (Boophilus) decolaratus, is a one-host tick that parasitizes cattle and vectors a debilitating protozoan parasite that causes babesiosis. Acaricidal properties of thyme were investigated on the cattle blue tick, R.B. decolaratus using various concentrations to establish the lethal dose concentration. The acaridal activity of thyme on ticks was monitored and recorded at two-hour intervals for 48 hours. Tick mortality was observed at all concentrations except the control. The lowest mortality rate was observed for 10μl/cm3 while 40μl/cm3 resulted in total tick mortality. The LC50 was established as 1,9μl/cm3. Significant differences (p<0.01) were observed between concentrations, however the mortality was directly proportional to thyme concentration. The thyme oil dehydrated the ticks leading to death within 48 hours. The study thus concludes that thyme oil can be used as a complimentary or alternative acaricide in an integrated livestock program or where organic means are enforced or to as a way of minimizing tolerance of ticks to conventional acaricides.
    VL  - 3
    IS  - 4
    ER  - 

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Author Information
  • Biological Sciences Department, Faculty of Science, Bindura University of Science Education, Bindura, Zimbabwe

  • Biological Sciences Department, Faculty of Science, Bindura University of Science Education, Bindura, Zimbabwe

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