A total of 10 species of fungi belonging to 5 genera were isolated and identified from four cereal grains; wheat, barley, rice and maize collected from three Libyan cities known to grain producers (Al-Zawia, Subratah and Tripoli) on 1% dextrose-Czapkes agar medium at 28 ± 2°C for 7-15 days using seed-plate method. Two species of Alternaria (A. raphani and A. tenusinae); two species of Aspergillus (A. flavus and A. niger); three Fusarium (F. graminearum, F. moniliforme and F. solani); one Rhizopus species (Rhizopus stolonifer) and two species of Penicillium (P. digitatum and P. notatum) were isolated from the grains. The densities of these fungi and their frequencies of occurrence have been investigated. It can be concluded that for human public health, cereal grains of production chain must be subjected to quality control and microbiological examinations.
| Published in | International Journal of Photochemistry and Photobiology (Volume 1, Issue 1) |
| DOI | 10.11648/j.ijpp.20170101.12 |
| Page(s) | 9-12 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Cereal Grains, Mycoflora, Seed-Plating Method
| [1] | Abramson, D., Hulasare, R., White, N. D. G., Jayas, D. S. and Marquardt, R. R. (1999). Mycotoxin Formation in Hulless Barley During Granary Storage at 15 and 90 Moisture Content. Journal of Food Stored Products Research, 35: 297-305. |
| [2] | Al-Defiery, M. E. and Merjan, A. F. (2015). Mycoflora of Mold Contamination in Wheat Flour and Storage Wheat Flour. Meso. Environ, J., 1: 18-25. |
| [3] | Almeida, A. P., Correa, B., Malllozzi, M. A. B., Sawazaki, E. and Soares, L. M. V. (2000). Mycoflora and Aflatoxin/Fomonision production by Fungal Isolates from Freshly Harvested Corn Hybrids. Brazilian Journal of Microbiology, 31: 321-326. |
| [4] | Domsch, K. H., Gams, W. and Anderson, T. H. (1981). Compendium of Soil Fungi Vol. 1 and 2, Academic Press, London. |
| [5] | Hernandez, E., Vila, R. and Garcia de la Cuadra, J. (1968). Microflora del Arroz Elaborado. 1: Flora de Molinos industrials Agroquimica Y Technologia de los Alimentos. 8 (2): 240-248. |
| [6] | International Seed Testing Association (1966). International rules for seed testing. Proc. Int. Test. Assoc., 32: 1-52. |
| [7] | Kacaniova, M. and Tancinova, D. (2001). Natural Occurrence of Fungi in Feeding Wheat in the Agricultural Farm Facilities. Act. Fytotechnica et Zootechnica, 4: 174-175. |
| [8] | Klich, M. (2001). Identification of Common Aspergillus species. United States Department of Agriculture. Agricultural Research Services, Southern Regional Research Center, New Orleans, LA. |
| [9] | Marei, A. Hamed, T. M. Abdel Ghany, Nadeem, I. Elhussieny and M. A. Nabih. (2016). Exploration of Fungal Infection in Agricultural Grains, Aflatoxin and Zeralenone Synthesis under pH Stress. Int. J. Curr. Microbiol. App. Sci., 5 (4): 1007-1017. |
| [10] | Marin, S., Sanchis, V., Saenz, R., Ramos, A. J., Vinas, I. and Magan, N. (1998). Ecological Determinants for Germination and Growth of Some Aspergillus and Penicillium spp. from Maize Grain. J. Appl. Microbiol., 84: 25-36. |
| [11] | Montes, G., Reyes, M., Montes, R. and Cantu, A. (2009). Incidence of Potentially Toxigenic Fungi in Maize (Zea mays L.) Grain Used as Food and Animal Feed. CYTA-J. Food, 7: 119-125. |
| [12] | Nelson, P. E., Toussoun, T. A. and Marasas, W. F. O. (1983). Fusarium species. An Illustrated Manual for Identification. The Penn. St. Univ. Press. Univ. Park, Pennslvania, 203. |
| [13] | Palumbo, J. D., Okeeffe, T. L., Abbas, H. K. (2008). Microbial Interaction With Mycotoxigenic Fungi and Mycotoxins. Toxin Rev., 27: 261-285. |
| [14] | Pitt, J. I. (1979). The Genus Penicillium and its Teleomorphic States Eupenicillium and Talaromyces. London Academic Press. London-UK. |
| [15] | Pitt, J. I. (1985). A laboratory guide to common Penicillium species. Commonwealth Scientific and Industrial Research Organization, Division of Food Research., 184. |
| [16] | Raper, K. B. and Fennel, D. I. (1977). The Genus Aspergillus. R. E. Krieger Publishing Company, Huntington, New Yourk. |
| [17] | Soliman, H. M. (2003). Mycoflora and Mycotoxins of Cereals Grains in Delta, Egypt. Microbiology, 31 (4): 183-190. |
| [18] | Tournas, V. and Katsoudas, E. (2008). Microbiological Quality of Various Medicinal Herbal Teas and Coffee Substitutes. Microbiology Insights, 1: 47. |
| [19] | Youssef, M. S., EL-Mahmoudy, E. M. and Abubakr A. S. (2008). Mesophilic Fungi and Mycotoxins Contamination of Libya Cultivated Four Fabaceae Seeds. Research Journal of Microbiology, 3 (7): 520-534. |
APA Style
Maryam A. S. Abubakr. (2017). Isolation and Identification of Fungi from Cereal Grains in Libya. International Journal of Photochemistry and Photobiology, 1(1), 9-12. https://doi.org/10.11648/j.ijpp.20170101.12
ACS Style
Maryam A. S. Abubakr. Isolation and Identification of Fungi from Cereal Grains in Libya. Int. J. Photochem. Photobiol. 2017, 1(1), 9-12. doi: 10.11648/j.ijpp.20170101.12
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ijpp.20170101.12,
author = {Maryam A. S. Abubakr},
title = {Isolation and Identification of Fungi from Cereal Grains in Libya},
journal = {International Journal of Photochemistry and Photobiology},
volume = {1},
number = {1},
pages = {9-12},
doi = {10.11648/j.ijpp.20170101.12},
url = {https://doi.org/10.11648/j.ijpp.20170101.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpp.20170101.12},
abstract = {A total of 10 species of fungi belonging to 5 genera were isolated and identified from four cereal grains; wheat, barley, rice and maize collected from three Libyan cities known to grain producers (Al-Zawia, Subratah and Tripoli) on 1% dextrose-Czapkes agar medium at 28 ± 2°C for 7-15 days using seed-plate method. Two species of Alternaria (A. raphani and A. tenusinae); two species of Aspergillus (A. flavus and A. niger); three Fusarium (F. graminearum, F. moniliforme and F. solani); one Rhizopus species (Rhizopus stolonifer) and two species of Penicillium (P. digitatum and P. notatum) were isolated from the grains. The densities of these fungi and their frequencies of occurrence have been investigated. It can be concluded that for human public health, cereal grains of production chain must be subjected to quality control and microbiological examinations.},
year = {2017}
}
TY - JOUR T1 - Isolation and Identification of Fungi from Cereal Grains in Libya AU - Maryam A. S. Abubakr Y1 - 2017/02/21 PY - 2017 N1 - https://doi.org/10.11648/j.ijpp.20170101.12 DO - 10.11648/j.ijpp.20170101.12 T2 - International Journal of Photochemistry and Photobiology JF - International Journal of Photochemistry and Photobiology JO - International Journal of Photochemistry and Photobiology SP - 9 EP - 12 PB - Science Publishing Group SN - 2640-429X UR - https://doi.org/10.11648/j.ijpp.20170101.12 AB - A total of 10 species of fungi belonging to 5 genera were isolated and identified from four cereal grains; wheat, barley, rice and maize collected from three Libyan cities known to grain producers (Al-Zawia, Subratah and Tripoli) on 1% dextrose-Czapkes agar medium at 28 ± 2°C for 7-15 days using seed-plate method. Two species of Alternaria (A. raphani and A. tenusinae); two species of Aspergillus (A. flavus and A. niger); three Fusarium (F. graminearum, F. moniliforme and F. solani); one Rhizopus species (Rhizopus stolonifer) and two species of Penicillium (P. digitatum and P. notatum) were isolated from the grains. The densities of these fungi and their frequencies of occurrence have been investigated. It can be concluded that for human public health, cereal grains of production chain must be subjected to quality control and microbiological examinations. VL - 1 IS - 1 ER -
Information
Email: