The Qualitative and Quantitative Phytochemical Investigation of Crinum Species in Ethiopia
International Journal of Photochemistry and Photobiology
Volume 3, Issue 1, June 2019, Pages: 1-9
Received: May 13, 2019; Accepted: Jun. 13, 2019; Published: Jul. 2, 2019
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Authors
Asnakech Senbeta, Crop & Horticulture Biodiversity Directorate, Ethiopian Biodiversity Institute, Addis Ababa, Ethiopia
Tesfaye Awas, Forest & Range Land Plants Biodiversity Directorate, Ethiopian Biodiversity Institute, Addis Ababa, Ethiopia
Abdella Gure, Department of Forestry, Wondo Genet College of Forestry & Natural Resources, Hawassa Universities, Wondo Genet, Ethiopia
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Abstract
Medicinal plants have a long history of use in most communities all over the world. Plants have the ability to synthesize a wide diversity of chemical compounds that uses to perform important biological functions. Many of Genus Crinum has been broadly used in traditional and ethno-medicines in the world wide. The aims of this study were to investigate the qualitative and quantitative phytochemicals constituents of the four species of the genus Crinum that exists in Ethiopia. All experiments were follow standard procedures. For the purpose of conducting phytochemical analyses on the four species each, three to six bulbs were collected from Field Gene Banks, Botanical Gardens and local fields. The bulb samples were cleaned, dried and crushed into powder. In this study the cold extraction methods were used the extraction solvents such as: n-hexane, ethanol, methanol and water. As a result of the phytochemical analyses, it revealed the presence of alkaloids, flavonoids, saponins, tannins and phenols in Crinum abyssinicum and Crinum bambusetum species. Likewise, it confirmed the presence of alkaloids, flavonoids, phenols and tannins in Crinum macowanii. Moreover, it confirmed the presence of alkaloids, flavonoids, phenols and tannins Crinum ornatum. The chemical constituents revealed the presence of relatively high concentration of alkaloids (9.66%), saponins (19.72%), phenols (10.33%), and tannins (0.61%) in the bulbs of Crinum bambusetum. Similarly, the highest concentration of flavonoids (27.72%) was recorded from the bulbs of Crinum ornatum. As more phytochemicals constituents are being identified and tested, traditional uses of the Crinum are being verified. Accordingly, the evidence on the chemical constituents of the species explains the uses of the plants. Therefore, it is worthwhile to recommend the use of the phytochemical constituents of the species studied for Pharmaceutical use in the treatment of different diseases.
Keywords
Crinum Abyssinicum, Crinum Bambusetum, Crinum Macowanii, Crinum Ornatum, Bulbs, Phytochemical Screening, Qualitative, Quantitative
To cite this article
Asnakech Senbeta, Tesfaye Awas, Abdella Gure, The Qualitative and Quantitative Phytochemical Investigation of Crinum Species in Ethiopia, International Journal of Photochemistry and Photobiology. Vol. 3, No. 1, 2019, pp. 1-9. doi: 10.11648/j.ijpp.20190301.11
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Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Sofowora A. Medicinal Plants and Traditional Medicine in Africa, 2nd Edition, Spectrum Books Limited (Publisher), Ibadan, Nigeria. 1993; 134-156.
[2]
Oloyede G, Oladosu I, Shodia A. Chemical composition and cytotoxicity of the essential oils of Crinum ornatum (Ait.) Bury. Afr. J. Pure and Appl. Chem. 2010; 4: 35-37.
[3]
Akintola A, Kehinde A, Adebiyi O, Ademowo O. Anti-tuberculosis activities of the crude methanolic extract and purified fractions of the bulb of Crinum jagus. Nigerian J. Physiolog. Sci. 2014; 28: 135-140.
[4]
Lawal A, Dangoggo S. Phytochemical, Proximate and Toxicity Studies of Aqueous Extract of Crinum ornatum (Toad’s Onion). Chem. Search Journal. 2014; 5: 45-50.
[5]
Nordal Inger and Sebsebe Demissew. Aloes and Lilies of Ethiopia and Eritrea January. 2010; 162-163.
[6]
Adesanya SA, Olugbade TA, Odebiyi OO, Aladesanmi JA. Antibactwrial Alkaloids in Crinum jagus. International J. Pharmacognosy. 1992; 4: 303-307.
[7]
Reta Regassa. Assessment of indigenous knowledge of medicinal plant practice and mode of service delivery in Hawassa city, southern Ethiopia. 2013; 531.
[8]
Firaol Tamiru, Waktole Terfa, Ejigu Kebede, Gizaw Dabessa, Rajeeb KR, Mekonnen Sorsa. Ethno-knowledge of plants used in veterinary practices in Dabo Hana District, West Ethiopia. Journal of Medicinal Plant Research. 2013; 7 (40): 2960-2971.
[9]
Haile Yinger, Ensermu Kelbessa, Tamrat Bekele, Ermias Lulekal. Plants used in traditional management of human ailments at Bale Mountains National Park, Southeastern Ethiopia. Journal of Medicinal Plants Research. 2008; 2 (6): 132-153
[10]
Hasler CM, Blumberg JB. Symposium on Phytochemicals: Biochemistry and Physiology. Journal of Nutrition. 1999; 129: 756S-757S.
[11]
Refaat J., Kamel M. S., Ramadan M. A. and Ali A. A (2013).Crinum; An Endless Source of Bioactive Principles: A Review. Part V. Biological Profile. IJPSR. 2013; 4 (4): 1239-1252.
[12]
Parekh J, Chanda, S. In Vitro Antibacterial Activity Of Crude Methanol Extract Of Woodfordia Fruticosa Kurz Flower (Lythacease). Brazilian Journal of Microbiology. 2007; 38: 2-3.
[13]
Parekh J, Nair R, Chanda S. Preliminary screening of some folkloric plants from western India for potential antimicrobial activity. Indian J Pharmacol. 2005.
[14]
Harborne JB. Phytochemical methods. A guide to modern techniques of plant analysis. Chapman and Hall, London. 1998; 5- 37
[15]
Harborne JB. Phytochemical methods. A guide to modern techniques of plant analysis. Chapman and Hall, London. 1973; 279: 49-188.
[16]
Ciulci I. Methodology for the Analysis of Vegetable Drugs. ChemicalIndustries Branch, Division of Industrial Operations. Unido, Romania. 1994; 24: 26-67.
[17]
Raman N. Phytochemical Methods, New Indian Publishing Agencies, New Delhi. 2006; 19.
[18]
Menghani E, Bhatnagar K, Saraswat P, Soni M. Isolation and characterization of bioactives from arid zone plants. International journal of pharmaceutical research and development. 2011; 4 (2): 113-118.
[19]
Misra, CS, Pratyush K, Dev MSL, James J, Veettil AKT, et al. A comparative study on phytochemical screening and antibacterial activity of roots of Alstoniascholaris with the roots, leaves and stem bark. International journal of resource phytochemistry and pharmacology. 2011; 1 (2): 77-82.
[20]
Obadoni BO, Ochuko PO. Phytochemical studies and comparative efficacy of the crude extracts of some homeostatic plants in Edo and Delta States of Nigeria. Global J. Pure Appl. Sci. 2001; 8: 203-208.
[21]
Boham BA, Kocipal-Abyazan R. Flavonoids and condensed tannins from leaves of Haiwaiian Vaccinium vaticulatum and V.calycinium. Pacific Sci. 1974; 48: 458-463.
[22]
Okwu DE, Josiah C. Evaluation of the chemical composition of two Nigerian Medical plants. Afr. J. Biotechnol. 2006; 5 (4): 357-361.
[23]
Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999; 299: 152-178.
[24]
Marinova D, Ribarov F, Atanassova M. Total Phenolics and Total Flavonoids in Bulgarian Fruits and Vegetables. J University Chem Technol Metallurgy. 2005; 40 (3): 255-260.
[25]
Rajeev Singh, Pawan Kumar Verma, Gagandeep Singh. Total phenolic, flavonoids and tannin contents in different extracts of Artemisia absinthium. J Intercult Ethnopharmacol. 2012; 1 (2): 101-104.
[26]
AfifyAel M, El-Beltagi HS, El-Salam SM, Omran AA. Biochemical changes in phenols, flavonoids, tannins, vitamin E, β-carotene and antioxidant activity during soaking of three white sorghum varieties. Asian Pac J Trop Biomed. 2012; 2 (3): 203-9.
[27]
Nair JJ, Machocho AK, Campbell WE, Brun R, Viladomat F, et al. Alkaloids from Crinum macowanii. Phytochemistry. 2000; 54 (8): 945–950.
[28]
Mugabo P, Obikeze KC, Njagi A, Burger PA, Green I, et al. Cardiovascular Effects of the Alkaloid Hippadine On the isolated perfused rat heart. 2012; 2 (1): 172 -177.
[29]
Kouam, MF, Flavien AAT, Jean DW, Alain FKW, Neil AK, et al. 2014.
[30]
Mohammed ZK, Modu, SAD, Olukade MF, Fatimah SS, Falmata AS, etal. Acute Toxicity Study on the Bulb of Crinum ornatum (Ait Bury).Bull. Env. Pharmacol. Life Sci. 2014; 3 (9): 168-171.
[31]
Ganiyat KO, Yunus R. Preliminary Anticonvulsant Screening of Crinum Ornatum Bulbs extract, African Journal of Basic & Applied Sciences. 2011; 3 (5): 235-238.
[32]
Tram NT, Titorenkova T, Bankova V, Handjieva N, Popov SS. Crinum L. Amaryllidaceae. Fitoterapia. 2002; 73 (3): 183-208.
[33]
Mvongo C, Noubissi PA, Kamgang R, Minka CS, Mfopa A, et al. Phytochemical Studies and In Vitro Antioxidant Potential of Two Different Extracts of Crinum Jagus. Int J Pharm Sci Res. 2015; 6 (6): 2354-59. doi: 10. 13040/IJPSR.0975-8232.
[34]
Shilpa. K, Rajendra Y, Sanjeeva KA, Vinay KD, Vinod KR, et al. Evaluation of Wound Healing Potential in Crinium defixum Ker Gawl Bulbs, Asian J Pharm Clin Res. 2013; 6 (1): 61-63.
[35]
Abubakar LL, Guan HT, Mohammed AA. Edibility and Medicinal Studies of Crinum ornatum in Comparison with Allium Sativum Pak. J. Nutr. 2015; 14 (11): 773-781.
[36]
Aziz A, Golam SR, Abhijit D, Jamiuddin A, Mizanur RM. Evaluation of In vitro Anthelmintic Activity, Total Phenolic Content and Cytotoxic Activity of Crinum latifolium L. (Family: Amaryllidaceae), Advanced Pharmaceutical Bulletin. 2014; 4 (1): 15-19.
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