International Journal of Food Science and Biotechnology

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Exploring the Effects of Biofield Energy Treated Vitamin D3 on Bone Health Parameters in MG-63 Cell Line

Received: 02 January 2018    Accepted: 12 January 2018    Published: 22 January 2018
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Abstract

The aim of the present experiment is to define the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen level, and bone mineralization. Both the test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples were received Consciousness Energy Healing Treatment by Rolando Baptista Piedad and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability of test samples using MTT assay showed that cell viability was more than 87% with safe and nontoxic profile on MG-63 cell line. ALP level was significantly increased by 196.1% (at 10 µg/mL) and 131.6% (at 0.1 µg/mL) in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively. In addition, ALP level was significantly increased by 342.1%, 205.2%, and 289.5% at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+BT-TI groups as compared with the untreated test item and DMEM group. The percentage of collagen level was significantly increased by 109.5%, 67.0%, and 100.9% at 0.1, 1, and 10 µg/mL, respectively in UT-DMEM+BT-TI group, while 228.4%, 157.8%, and 154.1% at 0.1, 1, and 10 µg/mL, respectively in BT-DMEM+UT-TI as compared with the untreated group. The percent of bone mineralization was significantly increased by 40.8% at 0.1 µg/mL in UT-DMEM+ BT-TI group, while 59.2% at 0.1 µg/mL in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 128% and 174.1% at 50 and 100 µg/mL, respectively as compared with the untreated group. Biofield Energy Treated vitamin D33 and DMEM would enhance the promotion and maintenance of strong and healthy bones. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of autoimmune diseases, adverse bone health conditions, comprising cancer, and bone disorders such as Rickets, Osteogenesis Imperfecta, Osteomalacia, Perthes' Disease, Fibrous Dysplasia, Osteomyelitis, Paget's Disease, and Osteoporosis.

DOI 10.11648/j.ijfsb.20180301.13
Published in International Journal of Food Science and Biotechnology (Volume 3, Issue 1, March 2018)
Page(s) 15-22
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Biofield Energy, Osteosarcoma Cells, Vitamin D, Osteoporosis, Bone Disorders, Bone Mineralization

References
[1] Holick MF (1996) Vitamin D and bone health. J Nutr 126: 1159S-64S.
[2] van Leeuwen JP, van Driel M, van den Bemd GJ, Pols HA (2001) Vitamin D control of osteoblast function and bone extracellular matrix mineralization. Crit Rev Eukaryot Gene Expr 11: 199-226.
[3] Bikle DD (2012) Vitamin D and bone. Curr Osteoporos Rep 10: 151-159.
[4] Lips P (2001) Vitamin D deficiency and secondary hyperparathyroidism in the elderly: consequences for bone loss and fractures and therapeutic implications. Endocrine Rev 22: 477-501.
[5] Hossein-nezhad A, Holick MF (2013) Vitamin D for health: A global perspective. Mayo Clinic proceedings Mayo Clinic 88: 720-755.
[6] Czekanska EM, Stoddart MJ, Richards RG, Hayes JS (2012) In search of an osteoblast cell model for in vitro research. Eur Cell Mater 24: 1-17.
[7] Luo XH, Liao EY (2003) Effects of estriol on the proliferation and differentiation of human osteoblastic MG-63 cells. Endocrine Res 29: 343-351.
[8] Iba K, Takada J, Yamashita T (2004) The serum level of bone-specific alkaline phosphatase activity is associated with aortic calcification in osteoporosis patients. J Bone Miner Metab 22: 594-596.
[9] Viguet-Carrin S, Garnero P, Delmas PD (2006) The role of collagen in bone strength. Osteoporos Int 17: 319-336.
[10] Bhattarai T, Bhattacharya K, Chaudhuri P, Sengupta P (2014) Correlation of common biochemical markers for bone turnover, serum calcium, and alkaline phosphatase in post-menopausal women. The Malaysian Journal of Medical Sciences : MJMS 21: 58-61.
[11] Rubik B (2002) The biofield hypothesis: Its biophysical basis and role in medicine. J Altern Complement Med 8: 703-717.
[12] Barnes PM, Bloom B, Nahin RL (2008) Complementary and alternative medicine use among adults and children: United States, 2007. Natl Health Stat Report 12: 1-23.
[13] Frass M, Strassl RP, Friehs H, Müllner M, Kundi M, Kaye AD (2012) Use and acceptance of complementary and alternative medicine among the general population and medical personnel: A Systematic Review. Ochsner J 12: 45-56.
[14] Trivedi MK, Tallapragada RM (2008) A transcendental to changing metal powder characteristics. Met Powder Rep 63: 22-28, 31.
[15] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O (2015) Studies of the atomic and crystalline characteristics of ceramic oxide nano powders after bio field treatment. Ind Eng Manage 4: 161.
[16] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) Effect of biofield energy treatment on physical and structural properties of calcium carbide and praseodymium oxide. International Journal of Materials Science and Applications 4: 390-395.
[17] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Morphological characterization, quality, yield and DNA fingerprinting of biofield energy treated alphonso mango (Mangifera indica L.). Journal of Food and Nutrition Sciences 3: 245-250.
[18] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of biochemical marker – Glutathione and DNA fingerprinting of biofield energy treated Oryza sativa. American Journal of BioScience 3: 243-248.
[19] Trivedi MK, Branton A, Trivedi D, Nayak G, Charan S, Jana S (2015) Phenotyping and 16S rDNA analysis after biofield treatment on Citrobacter braakii: A urinary pathogen. J Clin Med Genom 3: 129.
[20] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) Evaluation of biofield modality on viral load of Hepatitis B and C viruses. J Antivir Antiretrovir 7: 083-088.
[21] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) An impact of biofield treatment: Antimycobacterial susceptibility potential using BACTEC 460/MGIT-TB System. Mycobact Dis 5: 189.
[22] Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Phenotypic and biotypic characterization of Klebsiella oxytoca: An impact of biofield treatment. J Microb Biochem Technol 7: 203-206.
[23] Nayak G, Altekar N (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.
[24] Branton A, Jana S (2017) The influence of energy of consciousness healing treatment on low bioavailable resveratrol in male Sprague Dawley rats. International Journal of Clinical and Developmental Anatomy 3: 9-15.
[25] Branton A, Jana S (2017) The use of novel and unique biofield energy healing treatment for the improvement of poorly bioavailable compound, berberine in male Sprague Dawley rats. American Journal of Clinical and Experimental Medicine 5: 138-144.
[26] Branton A, Jana S (2017) Effect of The biofield energy healing treatment on the pharmacokinetics of 25-hydroxyvitamin D3 [25 (OH) D3] in rats after a single oral dose of vitamin D3. American Journal of Pharmacology and Phytotherapy 2: 11-18.
[27] Kinney JP, Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2017) Overall skin health potential of the biofield energy healing based herbomineral formulation using various skin parameters. American Journal of Life Sciences 5: 65-74.
[28] Singh J, Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2017) Consciousness energy healing treatment based herbomineral formulation: A safe and effective approach for skin health. American Journal of Pharmacology and Phytotherapy 2: 1-10.
[29] Trivedi MK, Branton A, Trivedi D, Nayak G, Plikerd WD, Surguy PL, Kock RJ, Piedad RB, Callas RP, Ansari SA, Barrett SL, Friedman S, Christie SL, Liu SC, Starling SE, Jones S, Allen SM, Wasmus SK, Benczik TA, Slade TC, Orban T, Vannes VL, Schlosser VM, Albino YSY, Panda P, Sethi KK, Jana S (2017) A Systematic study of the biofield energy healing treatment on physicochemical, thermal, structural, and behavioral properties of magnesium gluconate. International Journal of Bioorganic Chemistry 2: 135-145.
[30] Trivedi MK, Branton A, Trivedi D, Nayak G, Plikerd WD, Surguy PL, Kock RJ, Piedad RB, Callas RP, Ansari SA, Barrett SL, Friedman S, Christie SL, Liu SC, Starling SE, Jones S, Allen SM, Wasmus SK, Benczik TA, Slade TC, Orban T, Vannes VL, Schlosser VM, Albino YSY, Panda P, Sethi KK, Jana S (2017) Chromatographic and spectroscopic characterization of the consciousness energy healing treated Withania Somnifera (ashwagandha) root extract. European Journal of Biophysics 5: 38-47.
[31] Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol 4: 141.
[32] Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In vitro evaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. J Cancer Sci Ther 7: 253-257.
[33] Czekanska EM, Stoddart MJ, Richards RG, Hayes JS (2012) In search of an osteoblast cell model for in vitro research. Eur Cells Mater 24: 1-17.
[34] Biological evaluation of medical devices - Part 5: Tests for in vitro cytotoxicity (ISO 10993-5:2009), I. S. EN ISO, 10993-5:20093.
[35] Sharma U, Pal D, Prasad R (2014) Alkaline phosphatase: An overview. Indian J Clin Biochem 29: 269-278.
[36] Orimo H (2010) The mechanism of mineralization and the role of alkaline phosphatase in health and disease. J Nippon Med Sch 77: 4-12.
[37] Golub EE, Boesze-Battaglia K (2007) The role of alkaline phosphatase in mineralization. Curr Opin Orthop 18: 444-448.
[38] Black C, Clar C, Henderson R, MacEachern C, McNamee P, Quayyum Z, Royle P, Thomas S (2009) The clinical effectiveness of glucosamine and chondroitin supplements in slowing or arresting progression of osteoarthritis of the knee: A systematic review and economic evaluation. Health Technol Assess 13: 1-148.
[39] Lodish H, Berk A, Zipursky SL, et al. Molecular Cell Biology. 4th edition. New York: W. H. Freeman; 2000. Section 22.3, Collagen: The fibrous proteins of the matrix.
[40] Roschger P, Paschalis EP, Fratzl P, Klaushofer K (2008) Bone mineralization density distribution in health and disease. Bone 42: 456-466.
[41] Bikle DD (2014) Vitamin D metabolism, mechanism of action, and clinical applications. Chem Biol 21: 319-329.
Author Information
  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Global, Inc., Henderson, USA

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, India

Cite This Article
  • APA Style

    Rolando Baptista Piedad, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. (2018). Exploring the Effects of Biofield Energy Treated Vitamin D3 on Bone Health Parameters in MG-63 Cell Line. International Journal of Food Science and Biotechnology, 3(1), 15-22. https://doi.org/10.11648/j.ijfsb.20180301.13

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    ACS Style

    Rolando Baptista Piedad; Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak, et al. Exploring the Effects of Biofield Energy Treated Vitamin D3 on Bone Health Parameters in MG-63 Cell Line. Int. J. Food Sci. Biotechnol. 2018, 3(1), 15-22. doi: 10.11648/j.ijfsb.20180301.13

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    AMA Style

    Rolando Baptista Piedad, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, et al. Exploring the Effects of Biofield Energy Treated Vitamin D3 on Bone Health Parameters in MG-63 Cell Line. Int J Food Sci Biotechnol. 2018;3(1):15-22. doi: 10.11648/j.ijfsb.20180301.13

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  • @article{10.11648/j.ijfsb.20180301.13,
      author = {Rolando Baptista Piedad and Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Mayank Gangwar and Snehasis Jana},
      title = {Exploring the Effects of Biofield Energy Treated Vitamin D3 on Bone Health Parameters in MG-63 Cell Line},
      journal = {International Journal of Food Science and Biotechnology},
      volume = {3},
      number = {1},
      pages = {15-22},
      doi = {10.11648/j.ijfsb.20180301.13},
      url = {https://doi.org/10.11648/j.ijfsb.20180301.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijfsb.20180301.13},
      abstract = {The aim of the present experiment is to define the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen level, and bone mineralization. Both the test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples were received Consciousness Energy Healing Treatment by Rolando Baptista Piedad and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability of test samples using MTT assay showed that cell viability was more than 87% with safe and nontoxic profile on MG-63 cell line. ALP level was significantly increased by 196.1% (at 10 µg/mL) and 131.6% (at 0.1 µg/mL) in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively. In addition, ALP level was significantly increased by 342.1%, 205.2%, and 289.5% at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+BT-TI groups as compared with the untreated test item and DMEM group. The percentage of collagen level was significantly increased by 109.5%, 67.0%, and 100.9% at 0.1, 1, and 10 µg/mL, respectively in UT-DMEM+BT-TI group, while 228.4%, 157.8%, and 154.1% at 0.1, 1, and 10 µg/mL, respectively in BT-DMEM+UT-TI as compared with the untreated group. The percent of bone mineralization was significantly increased by 40.8% at 0.1 µg/mL in UT-DMEM+ BT-TI group, while 59.2% at 0.1 µg/mL in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 128% and 174.1% at 50 and 100 µg/mL, respectively as compared with the untreated group. Biofield Energy Treated vitamin D33 and DMEM would enhance the promotion and maintenance of strong and healthy bones. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of autoimmune diseases, adverse bone health conditions, comprising cancer, and bone disorders such as Rickets, Osteogenesis Imperfecta, Osteomalacia, Perthes' Disease, Fibrous Dysplasia, Osteomyelitis, Paget's Disease, and Osteoporosis.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Exploring the Effects of Biofield Energy Treated Vitamin D3 on Bone Health Parameters in MG-63 Cell Line
    AU  - Rolando Baptista Piedad
    AU  - Mahendra Kumar Trivedi
    AU  - Alice Branton
    AU  - Dahryn Trivedi
    AU  - Gopal Nayak
    AU  - Mayank Gangwar
    AU  - Snehasis Jana
    Y1  - 2018/01/22
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ijfsb.20180301.13
    DO  - 10.11648/j.ijfsb.20180301.13
    T2  - International Journal of Food Science and Biotechnology
    JF  - International Journal of Food Science and Biotechnology
    JO  - International Journal of Food Science and Biotechnology
    SP  - 15
    EP  - 22
    PB  - Science Publishing Group
    SN  - 2578-9643
    UR  - https://doi.org/10.11648/j.ijfsb.20180301.13
    AB  - The aim of the present experiment is to define the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen level, and bone mineralization. Both the test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples were received Consciousness Energy Healing Treatment by Rolando Baptista Piedad and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability of test samples using MTT assay showed that cell viability was more than 87% with safe and nontoxic profile on MG-63 cell line. ALP level was significantly increased by 196.1% (at 10 µg/mL) and 131.6% (at 0.1 µg/mL) in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively. In addition, ALP level was significantly increased by 342.1%, 205.2%, and 289.5% at 0.1, 1, and 10 µg/mL, respectively in the BT-DMEM+BT-TI groups as compared with the untreated test item and DMEM group. The percentage of collagen level was significantly increased by 109.5%, 67.0%, and 100.9% at 0.1, 1, and 10 µg/mL, respectively in UT-DMEM+BT-TI group, while 228.4%, 157.8%, and 154.1% at 0.1, 1, and 10 µg/mL, respectively in BT-DMEM+UT-TI as compared with the untreated group. The percent of bone mineralization was significantly increased by 40.8% at 0.1 µg/mL in UT-DMEM+ BT-TI group, while 59.2% at 0.1 µg/mL in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 128% and 174.1% at 50 and 100 µg/mL, respectively as compared with the untreated group. Biofield Energy Treated vitamin D33 and DMEM would enhance the promotion and maintenance of strong and healthy bones. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of autoimmune diseases, adverse bone health conditions, comprising cancer, and bone disorders such as Rickets, Osteogenesis Imperfecta, Osteomalacia, Perthes' Disease, Fibrous Dysplasia, Osteomyelitis, Paget's Disease, and Osteoporosis.
    VL  - 3
    IS  - 1
    ER  - 

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