Comparative Evaluation of Glibenclamide and Insulin on the Pups’ Liver Cytoarchitectonic Properties and Some Dams’ Parameters in Pregnant Streptozotocin-Induced Diabetic Rats
American Journal of Psychiatry and Neuroscience
Volume 6, Issue 1, March 2018, Pages: 9-14
Received: Nov. 21, 2017; Accepted: Dec. 6, 2017; Published: Jan. 11, 2018
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Authors
Lawal e Sodiq Kolawol, Department of Anatomy, St. Francis University College of Health Sciences and Allied Sciences, Ifakara, Tanzania; Department of Anatomy, Western Campus, Kampala International University, Ishaka-Bushenyi, Uganda
Adeniji Adeoluwa Akeem, Department of Anatomy, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria
Sangoyomi Oluwaseun Adewoye, Department of Anatomy, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria
Adeyemo Rasheed Omotayo, Department of Anatomy, Western Campus, Kampala International University, Ishaka-Bushenyi, Uganda
Buhari Muhammad Olanrewaju, Department of Anatomy, Western Campus, Kampala International University, Ishaka-Bushenyi, Uganda
Sulaiman Sheu Oluwadare, Department of Physiology, Faculty of Medicine, Kampala International University in Tanzania, Dar es Salaam, Tanzania
Osinubi Abraham Adewale, Department of Anatomy, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria
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Abstract
Despite the significant achievements in the treatment modalities and preventive measures, the prevalence of gestational diabetes in Africa has continued to rise exponentially in the last few decades. There is growing concern on the use of oral hypoglycemic agents (OHAs) during pregnancy, due to the potential of the agents in causing adverse effect (s) on the developing fetus and its effectiveness in managing the gestational diabetes mellitus. The objective of this study was to investigate the action of glibenclamide compared with insulin on pups’ liver cytoarchitectonic property and oxidative stress markers, and on maternal glucose level and sexual hormonal profile. Twenty pregnant female Sprague-Dawley rats (120-160 g) divided into 4 groups A, B, C and D (n=5 per group) were used for the study. Rats in group A (control) were given 0.5ml distilled water daily while the rats in groups B, C, and D were rendered diabetic by administration of intraperitoneal low-dose streptozotocin (STZ) and subsequently treated with 0.5mls of distilled water, glibenclamide (0.29 mg/kg body weight) and insulin (1 UI daily) respectively. Blood glucose levels were monitored and recorded throughout the experiment. The rats were sacrificed on the 19th day of gestational period. The pups’ liver and maternal blood sample were collected for analysis. The glibenclamide and insulin groups showed significant (p≤0.05) decreased in blood glucose with an increased maternal body weight when compared to the diabetic group. The activities of GSH, SOD and CAT were significantly increased (p≤0.05) in the glibenclamide and insulin treated groups compared to the diabetic group. Also, MDA significantly reduced in the glibenclamide and insulin treated groups (C & D) when compared to the diabetic untreated group (B) with the greater reduction observed for insulin. There was an improvement in the hormonal profiles of glibenclamide and insulin treated groups compared with the diabetic group. Histologically, glibenclamide and insulin showed an improvement in the arrangement of cytoarchitectonic property of pups’ liver with mild steatosis compared with diabetic group. Based on our observations in this study, it was concluded that glibenclamide is as effective as insulin with no or little negative effect and could be an optional drug to be used in the treatment of gestational diabetes mellitus in place of insulin.
Keywords
Cytoarchitectonic, Gestational Maternal Diabetes, Glibenclamide, Pups, Dams
To cite this article
Lawal e Sodiq Kolawol, Adeniji Adeoluwa Akeem, Sangoyomi Oluwaseun Adewoye, Adeyemo Rasheed Omotayo, Buhari Muhammad Olanrewaju, Sulaiman Sheu Oluwadare, Osinubi Abraham Adewale, Comparative Evaluation of Glibenclamide and Insulin on the Pups’ Liver Cytoarchitectonic Properties and Some Dams’ Parameters in Pregnant Streptozotocin-Induced Diabetic Rats, American Journal of Psychiatry and Neuroscience. Vol. 6, No. 1, 2018, pp. 9-14. doi: 10.11648/j.ajpn.20180601.12
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Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Aguwa CN. Diabetes mellitus In: Therapeutic basis of climate pharmacy in the tropics. Optimal publishers, Enugu, Nigeria 1996; 1-453.
[2]
Kalra B, Gupta Y, Singla R, Kalra S. Use of oral anti-diabetic agents in pregnancy: A pragmatic approach. North Am J Med Sci 2015; (7): 6-12.
[3]
Kuwata C, Saeki N, Honda K, Matsuoka T, Tsuchiya Y, Shimomura K. Effects of Maternal Hypoglycemia on Fetal Eye and Skeleton Development in Rats. Reproductive Toxicology 2017; http://dx.doi.org/10.1016/j.reprotox.2017.05.009.
[4]
Bennett WL, Robinson KA, Saldenha IJ, Wilson LM, Nilcholson WK. High priority research needs for gestational diabetes mellitus. J. womens health 2012; (1): 925-32.
[5]
Nwanjo HU, Nwokoro EA. Antidiabetic and biochemical effects of aqueous extract of vernonia amygdalina leaf in normoglycaemic and diabetic rats. J Inno Life Sci 2004; (7): 6-10.
[6]
World Health Organisation (WHO), Facts Sheets to the Ministerial Technical Working Group (TWG) on the United Nations High Level meeting on Non-communicable Diseases ( NCDs) WHO Representative for Nigeria ( Dr. David Okello). Abuja July 2011.
[7]
Ogunmodede OS, Oseni SO, Akinbisoye AA, Adenmosun OO. Dracaena arborea leaf extract: A phytotherapeutic option for ameliorating oxidative stress-mediated endocrine and testicular disorders in alloxan-induced diabetic rats. Journal of Coastal Life Medicine 2015; 3 (10): 809-814.
[8]
Harrison SA, Brunt EM, Goodman ZD, Di BIsceglie AM. Diabetic hepatosclerosis: diabetic microangiopathy of the liver. Archives of pathology and laboratory Medicine 2006; (16): 271-274.
[9]
Bell, D. S & Allbright, E. The multifaceted association of hepatobiliary disease and diabetes. Endocrine Practice 2007 13, 284-289.
[10]
Gbotolorun SC, Oremosu AA, Osinubi AAA, Noronha CC, Coker HAB, Silva BO. Ameliorative effect of vitamin E on the deleterious effect of Amodiaquine hydrochloride (AQ. HCL) on the reproductive function of the adult cyclic Sprague-Dawley (S-D) rats. Biology and Medicine 2012; 4 (3): 141-146.
[11]
Byer SL, Wiles MV, Dun SL, Taft RA. Mouse Estrous cycle identification tool and images. Plos ONE 2012; 7 (4): 1360-1371.
[12]
Szkudelski T. The mechansim of alloxan and streptozotocin action in B cells of the rats’ pancreas Physiol. Res 2001; (50): 536-546.
[13]
Ding, S, Shen Z, Chen Y, Sun S, Liu Q, Xie M. Pioglitazone can ameliorate insulin resistance in low-dose streptozotocin and high sucrose-fat diet induced obese rats Acta Pharmacologian Sinica 2015., 26 (5), 575-580.
[14]
Guariguata L, Linnen Kamp U, Beagley J, Whiting DR, Cho NH. Global estimates of the prevalence of hyperglyceamic in pregnancy. Diabetes Res Clin. Pract 2014; (103): 176-85.
[15]
Bharti Kalra, YashGupta, Rajir Singla and Sanjay. Use of Oral anti-diabetic agents in pregnancy: A pragmatic Approval. N Am. J. Med Sci 2015., 7 (1): 6-12. PMC 4325398.
[16]
Levri KM, Slaymaker E, Last A. Metformin treatment for over-weight and obese adults: a system review. Ann Fam Med 2005; (3) 457-461.
[17]
Montserrat Balsells, Apoloria Garcia-Pati, Marta Rogue, ignasi G, Rosa Corloy. Glibenclamide, metformin and insulin for the treatment of gestational diabetes. A systematic review and meta-analysis. BMJ 2015; 350-355 doi. http//dx.doi.org.
[18]
Ogunmodede OS, Oseni SO, Oyekan JO, Lawal SK, Adeoye OA. In vivo Studies on the Phytotherapeutic and Fertility Effects of Dracaena Arborea Extract in Alloxaninduced Type 1 Diabetic Male Rats Br J Med Med Res. 2016; 11 (5): 1-18.
[19]
Rehman Khan. weight gain & insulin therapy Br. J. diabetes vasc Dis 2004; (4): 264-7.
[20]
Gary Schenier M. S. Insulin and weight gain, BD diabetes (BD. com). Diabetes learning center 2006. 1-3.
[21]
Cotzee EJ, Jackson WPU. Oral hypoglycaemics in the first trimester and fetal outcomes. S. Afr. med J 1980; (61): 795-802.
[22]
Sripriya L, Vijayalakshmi M. altered lipid profile and antioxidant status of gestational Diabetes mellitus International Journal of Research in Pharmaceutical and Nano Sciences 2013. 2 (5): 622- 627 ISSN: 2319 – 9563.
[23]
Omotayo Owomofoyon Erejuwa, Siti Amrah Sulaiman, Mohd Suhaimi Abdul Wahab, Kuttulebbai Nainamohammed, et al., Antioxidant Protective Effect of Glibenclamide and Metformin in Combination with Honey in Pancreas of Streptozotocin-Induced Diabetic Rats Int. J. Mol. Sci 2010; (11) 2056-2066; ISSN 1422-0067 doi:10.3390/ijms11052056.
[24]
Elliot BD, Langer O, Schenker S, Johnson RF. insignificant transfer of glyburide coours across the human placenta. Am. J. Obster. Gynecol 1991; (165) 807-812.
[25]
Kinalski M, Sledziewski A, Telejko B, Kowalska I, Kretowski A, Zarzycki W, Kinalska I. Lipid peroxidation, antioxidant defence and acid – base stats in cord blood at birth: the influence of diabetes. Horm Metab Res 2001; (33): 227-231.
[26]
Tony Rydberg, Anders Jonsson, Michael Roder, Arne Melander, hypoglycemic activity of glyburide (glibenclamide) metabolites in humans. Diabetes Care 1994; 17 (9): 1-6.
[27]
Sugino N, Nakamura T, Ishimatsu M, Kato H. Changes in activities of superoxide dismutase and lipid peroxide in corpus luteum during pregnancy in rats. J Reprod Fertil 1993; 97: 347-351.
[28]
Selim F, Wael A, and Keith E. Jackson. Diabetes-Induced Reactive Oxygen Species. Mechanism of Their Generation and Role in Renal Injury. Journal of Diabetes Research. 2017, 1-30. https://doi.org/10.1155/2017/8379327.
[29]
Jihan Mohammed Mohieldin., Noon Babiker, Omer Mohamed Abdallah. Effect of type 2 diabetes mellitus on sudanese male fertility. sch. j. app. med. Sci 2016; 4 (6e): 2216-2223, ISSN 2320-669.
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