Comparative Evaluation of Glibenclamide and Insulin on the Pups’ Liver Cytoarchitectonic Properties and Some Dams’ Parameters in Pregnant Streptozotocin-Induced Diabetic Rats

Lawal e Sodiq Kolawol; Adeniji Adeoluwa Akeem; Sangoyomi Oluwaseun Adewoye; Adeyemo Rasheed Omotayo; Buhari Muhammad Olanrewaju; Sulaiman Sheu Oluwadare; Osinubi Abraham Adewale

doi:doi:10.11648/j.ajpn.20180601.12

Comparative Evaluation of Glibenclamide and Insulin on the Pups’ Liver Cytoarchitectonic Properties and Some Dams’ Parameters in Pregnant Streptozotocin-Induced Diabetic Rats

Lawal e Sodiq Kolawol, Adeniji Adeoluwa Akeem, Sangoyomi Oluwaseun Adewoye, Adeyemo Rasheed Omotayo, Buhari Muhammad Olanrewaju, Sulaiman Sheu Oluwadare, Osinubi Abraham Adewale

Published in American Journal of Psychiatry and Neuroscience (Volume 6, Issue 1)

Received: 21 November 2017 Accepted: 6 December 2017 Published: 11 January 2018

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Abstract

Despite the significant achievements in the treatment modalities and preventive measures, the prevalence of gestational diabetes in Africa has continued to rise exponentially in the last few decades. There is growing concern on the use of oral hypoglycemic agents (OHAs) during pregnancy, due to the potential of the agents in causing adverse effect (s) on the developing fetus and its effectiveness in managing the gestational diabetes mellitus. The objective of this study was to investigate the action of glibenclamide compared with insulin on pups’ liver cytoarchitectonic property and oxidative stress markers, and on maternal glucose level and sexual hormonal profile. Twenty pregnant female Sprague-Dawley rats (120-160 g) divided into 4 groups A, B, C and D (n=5 per group) were used for the study. Rats in group A (control) were given 0.5ml distilled water daily while the rats in groups B, C, and D were rendered diabetic by administration of intraperitoneal low-dose streptozotocin (STZ) and subsequently treated with 0.5mls of distilled water, glibenclamide (0.29 mg/kg body weight) and insulin (1 UI daily) respectively. Blood glucose levels were monitored and recorded throughout the experiment. The rats were sacrificed on the 19^th day of gestational period. The pups’ liver and maternal blood sample were collected for analysis. The glibenclamide and insulin groups showed significant (p≤0.05) decreased in blood glucose with an increased maternal body weight when compared to the diabetic group. The activities of GSH, SOD and CAT were significantly increased (p≤0.05) in the glibenclamide and insulin treated groups compared to the diabetic group. Also, MDA significantly reduced in the glibenclamide and insulin treated groups (C & D) when compared to the diabetic untreated group (B) with the greater reduction observed for insulin. There was an improvement in the hormonal profiles of glibenclamide and insulin treated groups compared with the diabetic group. Histologically, glibenclamide and insulin showed an improvement in the arrangement of cytoarchitectonic property of pups’ liver with mild steatosis compared with diabetic group. Based on our observations in this study, it was concluded that glibenclamide is as effective as insulin with no or little negative effect and could be an optional drug to be used in the treatment of gestational diabetes mellitus in place of insulin.

Published in	American Journal of Psychiatry and Neuroscience (Volume 6, Issue 1)
DOI	10.11648/j.ajpn.20180601.12
Page(s)	9-14
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Cytoarchitectonic, Gestational Maternal Diabetes, Glibenclamide, Pups, Dams

References

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[3]	Kuwata C, Saeki N, Honda K, Matsuoka T, Tsuchiya Y, Shimomura K. Effects of Maternal Hypoglycemia on Fetal Eye and Skeleton Development in Rats. Reproductive Toxicology 2017; http://dx.doi.org/10.1016/j.reprotox.2017.05.009.
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[7]	Ogunmodede OS, Oseni SO, Akinbisoye AA, Adenmosun OO. Dracaena arborea leaf extract: A phytotherapeutic option for ameliorating oxidative stress-mediated endocrine and testicular disorders in alloxan-induced diabetic rats. Journal of Coastal Life Medicine 2015; 3 (10): 809-814.
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[17]	Montserrat Balsells, Apoloria Garcia-Pati, Marta Rogue, ignasi G, Rosa Corloy. Glibenclamide, metformin and insulin for the treatment of gestational diabetes. A systematic review and meta-analysis. BMJ 2015; 350-355 doi. http//dx.doi.org.
[18]	Ogunmodede OS, Oseni SO, Oyekan JO, Lawal SK, Adeoye OA. In vivo Studies on the Phytotherapeutic and Fertility Effects of Dracaena Arborea Extract in Alloxaninduced Type 1 Diabetic Male Rats Br J Med Med Res. 2016; 11 (5): 1-18.
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[23]	Omotayo Owomofoyon Erejuwa, Siti Amrah Sulaiman, Mohd Suhaimi Abdul Wahab, Kuttulebbai Nainamohammed, et al., Antioxidant Protective Effect of Glibenclamide and Metformin in Combination with Honey in Pancreas of Streptozotocin-Induced Diabetic Rats Int. J. Mol. Sci 2010; (11) 2056-2066; ISSN 1422-0067 doi:10.3390/ijms11052056.
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Lawal e Sodiq Kolawol, Adeniji Adeoluwa Akeem, Sangoyomi Oluwaseun Adewoye, Adeyemo Rasheed Omotayo, Buhari Muhammad Olanrewaju, et al. (2018). Comparative Evaluation of Glibenclamide and Insulin on the Pups’ Liver Cytoarchitectonic Properties and Some Dams’ Parameters in Pregnant Streptozotocin-Induced Diabetic Rats. American Journal of Psychiatry and Neuroscience, 6(1), 9-14. https://doi.org/10.11648/j.ajpn.20180601.12

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ACS Style

Lawal e Sodiq Kolawol; Adeniji Adeoluwa Akeem; Sangoyomi Oluwaseun Adewoye; Adeyemo Rasheed Omotayo; Buhari Muhammad Olanrewaju, et al. Comparative Evaluation of Glibenclamide and Insulin on the Pups’ Liver Cytoarchitectonic Properties and Some Dams’ Parameters in Pregnant Streptozotocin-Induced Diabetic Rats. Am. J. Psychiatry Neurosci. 2018, 6(1), 9-14. doi: 10.11648/j.ajpn.20180601.12

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AMA Style

Lawal e Sodiq Kolawol, Adeniji Adeoluwa Akeem, Sangoyomi Oluwaseun Adewoye, Adeyemo Rasheed Omotayo, Buhari Muhammad Olanrewaju, et al. Comparative Evaluation of Glibenclamide and Insulin on the Pups’ Liver Cytoarchitectonic Properties and Some Dams’ Parameters in Pregnant Streptozotocin-Induced Diabetic Rats. Am J Psychiatry Neurosci. 2018;6(1):9-14. doi: 10.11648/j.ajpn.20180601.12

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@article{10.11648/j.ajpn.20180601.12,
  author = {Lawal e Sodiq Kolawol and Adeniji Adeoluwa Akeem and Sangoyomi Oluwaseun Adewoye and Adeyemo Rasheed Omotayo and Buhari Muhammad Olanrewaju and Sulaiman Sheu Oluwadare and Osinubi Abraham Adewale},
  title = {Comparative Evaluation of Glibenclamide and Insulin on the Pups’ Liver Cytoarchitectonic Properties and Some Dams’ Parameters in Pregnant Streptozotocin-Induced Diabetic Rats},
  journal = {American Journal of Psychiatry and Neuroscience},
  volume = {6},
  number = {1},
  pages = {9-14},
  doi = {10.11648/j.ajpn.20180601.12},
  url = {https://doi.org/10.11648/j.ajpn.20180601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpn.20180601.12},
  abstract = {Despite the significant achievements in the treatment modalities and preventive measures, the prevalence of gestational diabetes in Africa has continued to rise exponentially in the last few decades. There is growing concern on the use of oral hypoglycemic agents (OHAs) during pregnancy, due to the potential of the agents in causing adverse effect (s) on the developing fetus and its effectiveness in managing the gestational diabetes mellitus. The objective of this study was to investigate the action of glibenclamide compared with insulin on pups’ liver cytoarchitectonic property and oxidative stress markers, and on maternal glucose level and sexual hormonal profile. Twenty pregnant female Sprague-Dawley rats (120-160 g) divided into 4 groups A, B, C and D (n=5 per group) were used for the study. Rats in group A (control) were given 0.5ml distilled water daily while the rats in groups B, C, and D were rendered diabetic by administration of intraperitoneal low-dose streptozotocin (STZ) and subsequently treated with 0.5mls of distilled water, glibenclamide (0.29 mg/kg body weight) and insulin (1 UI daily) respectively. Blood glucose levels were monitored and recorded throughout the experiment. The rats were sacrificed on the 19th day of gestational period. The pups’ liver and maternal blood sample were collected for analysis. The glibenclamide and insulin groups showed significant (p≤0.05) decreased in blood glucose with an increased maternal body weight when compared to the diabetic group. The activities of GSH, SOD and CAT were significantly increased (p≤0.05) in the glibenclamide and insulin treated groups compared to the diabetic group. Also, MDA significantly reduced in the glibenclamide and insulin treated groups (C & D) when compared to the diabetic untreated group (B) with the greater reduction observed for insulin. There was an improvement in the hormonal profiles of glibenclamide and insulin treated groups compared with the diabetic group. Histologically, glibenclamide and insulin showed an improvement in the arrangement of cytoarchitectonic property of pups’ liver with mild steatosis compared with diabetic group. Based on our observations in this study, it was concluded that glibenclamide is as effective as insulin with no or little negative effect and could be an optional drug to be used in the treatment of gestational diabetes mellitus in place of insulin.},
 year = {2018}
}

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TY  - JOUR
T1  - Comparative Evaluation of Glibenclamide and Insulin on the Pups’ Liver Cytoarchitectonic Properties and Some Dams’ Parameters in Pregnant Streptozotocin-Induced Diabetic Rats
AU  - Lawal e Sodiq Kolawol
AU  - Adeniji Adeoluwa Akeem
AU  - Sangoyomi Oluwaseun Adewoye
AU  - Adeyemo Rasheed Omotayo
AU  - Buhari Muhammad Olanrewaju
AU  - Sulaiman Sheu Oluwadare
AU  - Osinubi Abraham Adewale
Y1  - 2018/01/11
PY  - 2018
N1  - https://doi.org/10.11648/j.ajpn.20180601.12
DO  - 10.11648/j.ajpn.20180601.12
T2  - American Journal of Psychiatry and Neuroscience
JF  - American Journal of Psychiatry and Neuroscience
JO  - American Journal of Psychiatry and Neuroscience
SP  - 9
EP  - 14
PB  - Science Publishing Group
SN  - 2330-426X
UR  - https://doi.org/10.11648/j.ajpn.20180601.12
AB  - Despite the significant achievements in the treatment modalities and preventive measures, the prevalence of gestational diabetes in Africa has continued to rise exponentially in the last few decades. There is growing concern on the use of oral hypoglycemic agents (OHAs) during pregnancy, due to the potential of the agents in causing adverse effect (s) on the developing fetus and its effectiveness in managing the gestational diabetes mellitus. The objective of this study was to investigate the action of glibenclamide compared with insulin on pups’ liver cytoarchitectonic property and oxidative stress markers, and on maternal glucose level and sexual hormonal profile. Twenty pregnant female Sprague-Dawley rats (120-160 g) divided into 4 groups A, B, C and D (n=5 per group) were used for the study. Rats in group A (control) were given 0.5ml distilled water daily while the rats in groups B, C, and D were rendered diabetic by administration of intraperitoneal low-dose streptozotocin (STZ) and subsequently treated with 0.5mls of distilled water, glibenclamide (0.29 mg/kg body weight) and insulin (1 UI daily) respectively. Blood glucose levels were monitored and recorded throughout the experiment. The rats were sacrificed on the 19th day of gestational period. The pups’ liver and maternal blood sample were collected for analysis. The glibenclamide and insulin groups showed significant (p≤0.05) decreased in blood glucose with an increased maternal body weight when compared to the diabetic group. The activities of GSH, SOD and CAT were significantly increased (p≤0.05) in the glibenclamide and insulin treated groups compared to the diabetic group. Also, MDA significantly reduced in the glibenclamide and insulin treated groups (C & D) when compared to the diabetic untreated group (B) with the greater reduction observed for insulin. There was an improvement in the hormonal profiles of glibenclamide and insulin treated groups compared with the diabetic group. Histologically, glibenclamide and insulin showed an improvement in the arrangement of cytoarchitectonic property of pups’ liver with mild steatosis compared with diabetic group. Based on our observations in this study, it was concluded that glibenclamide is as effective as insulin with no or little negative effect and could be an optional drug to be used in the treatment of gestational diabetes mellitus in place of insulin.
VL  - 6
IS  - 1
ER  -

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Author Information

Lawal e Sodiq Kolawol

Department of Anatomy, St. Francis University College of Health Sciences and Allied Sciences, Ifakara, Tanzania; Department of Anatomy, Western Campus, Kampala International University, Ishaka-Bushenyi, Uganda
Adeniji Adeoluwa Akeem

Department of Anatomy, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria
Sangoyomi Oluwaseun Adewoye

Department of Anatomy, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria
Adeyemo Rasheed Omotayo

Department of Anatomy, Western Campus, Kampala International University, Ishaka-Bushenyi, Uganda
Buhari Muhammad Olanrewaju

Department of Anatomy, Western Campus, Kampala International University, Ishaka-Bushenyi, Uganda
Sulaiman Sheu Oluwadare

Department of Physiology, Faculty of Medicine, Kampala International University in Tanzania, Dar es Salaam, Tanzania
Osinubi Abraham Adewale

Department of Anatomy, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria

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APA Style

Lawal e Sodiq Kolawol, Adeniji Adeoluwa Akeem, Sangoyomi Oluwaseun Adewoye, Adeyemo Rasheed Omotayo, Buhari Muhammad Olanrewaju, et al. (2018). Comparative Evaluation of Glibenclamide and Insulin on the Pups’ Liver Cytoarchitectonic Properties and Some Dams’ Parameters in Pregnant Streptozotocin-Induced Diabetic Rats. American Journal of Psychiatry and Neuroscience, 6(1), 9-14. https://doi.org/10.11648/j.ajpn.20180601.12

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ACS Style

Lawal e Sodiq Kolawol; Adeniji Adeoluwa Akeem; Sangoyomi Oluwaseun Adewoye; Adeyemo Rasheed Omotayo; Buhari Muhammad Olanrewaju, et al. Comparative Evaluation of Glibenclamide and Insulin on the Pups’ Liver Cytoarchitectonic Properties and Some Dams’ Parameters in Pregnant Streptozotocin-Induced Diabetic Rats. Am. J. Psychiatry Neurosci. 2018, 6(1), 9-14. doi: 10.11648/j.ajpn.20180601.12

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AMA Style

Lawal e Sodiq Kolawol, Adeniji Adeoluwa Akeem, Sangoyomi Oluwaseun Adewoye, Adeyemo Rasheed Omotayo, Buhari Muhammad Olanrewaju, et al. Comparative Evaluation of Glibenclamide and Insulin on the Pups’ Liver Cytoarchitectonic Properties and Some Dams’ Parameters in Pregnant Streptozotocin-Induced Diabetic Rats. Am J Psychiatry Neurosci. 2018;6(1):9-14. doi: 10.11648/j.ajpn.20180601.12

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@article{10.11648/j.ajpn.20180601.12,
  author = {Lawal e Sodiq Kolawol and Adeniji Adeoluwa Akeem and Sangoyomi Oluwaseun Adewoye and Adeyemo Rasheed Omotayo and Buhari Muhammad Olanrewaju and Sulaiman Sheu Oluwadare and Osinubi Abraham Adewale},
  title = {Comparative Evaluation of Glibenclamide and Insulin on the Pups’ Liver Cytoarchitectonic Properties and Some Dams’ Parameters in Pregnant Streptozotocin-Induced Diabetic Rats},
  journal = {American Journal of Psychiatry and Neuroscience},
  volume = {6},
  number = {1},
  pages = {9-14},
  doi = {10.11648/j.ajpn.20180601.12},
  url = {https://doi.org/10.11648/j.ajpn.20180601.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpn.20180601.12},
  abstract = {Despite the significant achievements in the treatment modalities and preventive measures, the prevalence of gestational diabetes in Africa has continued to rise exponentially in the last few decades. There is growing concern on the use of oral hypoglycemic agents (OHAs) during pregnancy, due to the potential of the agents in causing adverse effect (s) on the developing fetus and its effectiveness in managing the gestational diabetes mellitus. The objective of this study was to investigate the action of glibenclamide compared with insulin on pups’ liver cytoarchitectonic property and oxidative stress markers, and on maternal glucose level and sexual hormonal profile. Twenty pregnant female Sprague-Dawley rats (120-160 g) divided into 4 groups A, B, C and D (n=5 per group) were used for the study. Rats in group A (control) were given 0.5ml distilled water daily while the rats in groups B, C, and D were rendered diabetic by administration of intraperitoneal low-dose streptozotocin (STZ) and subsequently treated with 0.5mls of distilled water, glibenclamide (0.29 mg/kg body weight) and insulin (1 UI daily) respectively. Blood glucose levels were monitored and recorded throughout the experiment. The rats were sacrificed on the 19th day of gestational period. The pups’ liver and maternal blood sample were collected for analysis. The glibenclamide and insulin groups showed significant (p≤0.05) decreased in blood glucose with an increased maternal body weight when compared to the diabetic group. The activities of GSH, SOD and CAT were significantly increased (p≤0.05) in the glibenclamide and insulin treated groups compared to the diabetic group. Also, MDA significantly reduced in the glibenclamide and insulin treated groups (C & D) when compared to the diabetic untreated group (B) with the greater reduction observed for insulin. There was an improvement in the hormonal profiles of glibenclamide and insulin treated groups compared with the diabetic group. Histologically, glibenclamide and insulin showed an improvement in the arrangement of cytoarchitectonic property of pups’ liver with mild steatosis compared with diabetic group. Based on our observations in this study, it was concluded that glibenclamide is as effective as insulin with no or little negative effect and could be an optional drug to be used in the treatment of gestational diabetes mellitus in place of insulin.},
 year = {2018}
}

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TY  - JOUR
T1  - Comparative Evaluation of Glibenclamide and Insulin on the Pups’ Liver Cytoarchitectonic Properties and Some Dams’ Parameters in Pregnant Streptozotocin-Induced Diabetic Rats
AU  - Lawal e Sodiq Kolawol
AU  - Adeniji Adeoluwa Akeem
AU  - Sangoyomi Oluwaseun Adewoye
AU  - Adeyemo Rasheed Omotayo
AU  - Buhari Muhammad Olanrewaju
AU  - Sulaiman Sheu Oluwadare
AU  - Osinubi Abraham Adewale
Y1  - 2018/01/11
PY  - 2018
N1  - https://doi.org/10.11648/j.ajpn.20180601.12
DO  - 10.11648/j.ajpn.20180601.12
T2  - American Journal of Psychiatry and Neuroscience
JF  - American Journal of Psychiatry and Neuroscience
JO  - American Journal of Psychiatry and Neuroscience
SP  - 9
EP  - 14
PB  - Science Publishing Group
SN  - 2330-426X
UR  - https://doi.org/10.11648/j.ajpn.20180601.12
AB  - Despite the significant achievements in the treatment modalities and preventive measures, the prevalence of gestational diabetes in Africa has continued to rise exponentially in the last few decades. There is growing concern on the use of oral hypoglycemic agents (OHAs) during pregnancy, due to the potential of the agents in causing adverse effect (s) on the developing fetus and its effectiveness in managing the gestational diabetes mellitus. The objective of this study was to investigate the action of glibenclamide compared with insulin on pups’ liver cytoarchitectonic property and oxidative stress markers, and on maternal glucose level and sexual hormonal profile. Twenty pregnant female Sprague-Dawley rats (120-160 g) divided into 4 groups A, B, C and D (n=5 per group) were used for the study. Rats in group A (control) were given 0.5ml distilled water daily while the rats in groups B, C, and D were rendered diabetic by administration of intraperitoneal low-dose streptozotocin (STZ) and subsequently treated with 0.5mls of distilled water, glibenclamide (0.29 mg/kg body weight) and insulin (1 UI daily) respectively. Blood glucose levels were monitored and recorded throughout the experiment. The rats were sacrificed on the 19th day of gestational period. The pups’ liver and maternal blood sample were collected for analysis. The glibenclamide and insulin groups showed significant (p≤0.05) decreased in blood glucose with an increased maternal body weight when compared to the diabetic group. The activities of GSH, SOD and CAT were significantly increased (p≤0.05) in the glibenclamide and insulin treated groups compared to the diabetic group. Also, MDA significantly reduced in the glibenclamide and insulin treated groups (C & D) when compared to the diabetic untreated group (B) with the greater reduction observed for insulin. There was an improvement in the hormonal profiles of glibenclamide and insulin treated groups compared with the diabetic group. Histologically, glibenclamide and insulin showed an improvement in the arrangement of cytoarchitectonic property of pups’ liver with mild steatosis compared with diabetic group. Based on our observations in this study, it was concluded that glibenclamide is as effective as insulin with no or little negative effect and could be an optional drug to be used in the treatment of gestational diabetes mellitus in place of insulin.
VL  - 6
IS  - 1
ER  -

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