Journal of Cancer Treatment and Research

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Effect of Storage at Temperature (4C) on Complete Blood Count Parameters

Received: 08 November 2016    Accepted: 17 March 2017    Published: 17 March 2017
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Abstract

Background: Complete blood counts are done to monitor overall health, to screen for some diseases, to confirm a diagnosis of some medical conditions, to monitor a medical condition, and to monitor changes in the body caused by medical treatments. Most blood counts today include a CBC count and leukocyte differential count (LDC) (that is, not just the total WBC count but also the count of each WBC type, such as neutrophils, eosinophils, basophils, monocytes, and lymphocytes). More sophisticated modern analyzers can provide extended differential counts. Objective: This study aimed to assess the effect of Storage and Temperature (4C) on Complete Blood Count Parameters. Materials and Methods: This were cross sectional study conducted in Khartoum state in 2016. It included 100 samples, complete blood count conducted to all 100 samples freshly, after 24hours, 48 hours and 72 hours. Mindary BC-2800 hematological analyzer was used to estimate CBC. Results: There was a significant difference in Hematocrit, mean cell volume, MCHC, red cell distribution width, Lymphocytes, granulocyte %, and platelets indices (P less than 0.05). Lymphocytes count, Mixed count, Lymphocytes %, Mixed % and PCT did not show statistically difference ant it were insignificant with P. values (P=0.067), (P=0.080), (P=0.072), (P=0.088) and (P=0.060) respectively. Conclusion: This study concluded that storage caused significant difference in Hematocrit, mean cell volume, MCHC, red cell distribution width, Lymphocytes, granulocyte %, and platelets indices (P less than 0.05).

DOI 10.11648/j.jctr.20170502.11
Published in Journal of Cancer Treatment and Research (Volume 5, Issue 2, March 2017)
Page(s) 7-10
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Complete Blood Count, Lymphocytes, WBCs

References
[1] Verso, ML (May 1962). "The Evolution of Blood Counting Techniques" (PDF). Read at a meeting of the Section of the History of Medicine, First Australian Medical Congress. 8: 149–58.
[2] Mayo Clinic (14 February 2014). "Complete blood count (CBC) Why it's done - Tests and Procedures". mayoclinic. org. Retrieved 29 July 2014.
[3] American Association of Blood Banks (24 April 2014), "Five Things Physicians and Patients Should Question", Choosing Wisely: an initiative of the ABIM Foundation, American Association of Blood Banks, retrieved 25 July 2014.
[4] Buttarello, M; Plebani, M (Jul 2008). "Automated blood cell counts: state of the art.". American journal of clinical pathology. 130 (1): 104–16.
[5] National Committee for Clinical Laboratory Standards. Tubes and additives for venous blood specimen collection; approved standard - fifth edition. Document H1-A5. Wayne, PA: NCCLS, 2003.
[6] National Committee for Clinical Laboratory Standards. Tubes and additives for venous blood specimen collection; approved standard - fifth edition. Document H1-A5. Wayne, PA: NCCLS, 2003.
[7] Registered: 10 June 2010; Accepted: 2 May 2011 [Kayıt Tarihi: 10 Haziran 2010; Kabul Tarihi: 02 Mayıs 2011].
[8] Kjeldsberg CR, Hershgold EJ. Spurious thrombocytopenia. JAMA. 1974; 227 (6): 628-30.
[9] Evans V. Platelet morphology and the blood smear. J Med Technol. 1984; 1: 689-95.
[10] Shreiner DP, Bell WR. Psueudothrombocytopenia: Manifestation of a new type of platelet agglutinin. Blood. 1973; 42: 541-49.
Author Information
  • Department of Haematology, Faculty of Medical Laboratory Sciences, Al neelin University, Khartoum, Sudan

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    Naif Taleb Ali. (2017). Effect of Storage at Temperature (4C) on Complete Blood Count Parameters. Journal of Cancer Treatment and Research, 5(2), 7-10. https://doi.org/10.11648/j.jctr.20170502.11

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    Naif Taleb Ali. Effect of Storage at Temperature (4C) on Complete Blood Count Parameters. J. Cancer Treat. Res. 2017, 5(2), 7-10. doi: 10.11648/j.jctr.20170502.11

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    AMA Style

    Naif Taleb Ali. Effect of Storage at Temperature (4C) on Complete Blood Count Parameters. J Cancer Treat Res. 2017;5(2):7-10. doi: 10.11648/j.jctr.20170502.11

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  • @article{10.11648/j.jctr.20170502.11,
      author = {Naif Taleb Ali},
      title = {Effect of Storage at Temperature (4C) on Complete Blood Count Parameters},
      journal = {Journal of Cancer Treatment and Research},
      volume = {5},
      number = {2},
      pages = {7-10},
      doi = {10.11648/j.jctr.20170502.11},
      url = {https://doi.org/10.11648/j.jctr.20170502.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.jctr.20170502.11},
      abstract = {Background: Complete blood counts are done to monitor overall health, to screen for some diseases, to confirm a diagnosis of some medical conditions, to monitor a medical condition, and to monitor changes in the body caused by medical treatments. Most blood counts today include a CBC count and leukocyte differential count (LDC) (that is, not just the total WBC count but also the count of each WBC type, such as neutrophils, eosinophils, basophils, monocytes, and lymphocytes). More sophisticated modern analyzers can provide extended differential counts. Objective: This study aimed to assess the effect of Storage and Temperature (4C) on Complete Blood Count Parameters. Materials and Methods: This were cross sectional study conducted in Khartoum state in 2016. It included 100 samples, complete blood count conducted to all 100 samples freshly, after 24hours, 48 hours and 72 hours. Mindary BC-2800 hematological analyzer was used to estimate CBC. Results: There was a significant difference in Hematocrit, mean cell volume, MCHC, red cell distribution width, Lymphocytes, granulocyte %, and platelets indices (P less than 0.05). Lymphocytes count, Mixed count, Lymphocytes %, Mixed % and PCT did not show statistically difference ant it were insignificant with P. values (P=0.067), (P=0.080), (P=0.072), (P=0.088) and (P=0.060) respectively. Conclusion: This study concluded that storage caused significant difference in Hematocrit, mean cell volume, MCHC, red cell distribution width, Lymphocytes, granulocyte %, and platelets indices (P less than 0.05).},
     year = {2017}
    }
    

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    N1  - https://doi.org/10.11648/j.jctr.20170502.11
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    JO  - Journal of Cancer Treatment and Research
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    AB  - Background: Complete blood counts are done to monitor overall health, to screen for some diseases, to confirm a diagnosis of some medical conditions, to monitor a medical condition, and to monitor changes in the body caused by medical treatments. Most blood counts today include a CBC count and leukocyte differential count (LDC) (that is, not just the total WBC count but also the count of each WBC type, such as neutrophils, eosinophils, basophils, monocytes, and lymphocytes). More sophisticated modern analyzers can provide extended differential counts. Objective: This study aimed to assess the effect of Storage and Temperature (4C) on Complete Blood Count Parameters. Materials and Methods: This were cross sectional study conducted in Khartoum state in 2016. It included 100 samples, complete blood count conducted to all 100 samples freshly, after 24hours, 48 hours and 72 hours. Mindary BC-2800 hematological analyzer was used to estimate CBC. Results: There was a significant difference in Hematocrit, mean cell volume, MCHC, red cell distribution width, Lymphocytes, granulocyte %, and platelets indices (P less than 0.05). Lymphocytes count, Mixed count, Lymphocytes %, Mixed % and PCT did not show statistically difference ant it were insignificant with P. values (P=0.067), (P=0.080), (P=0.072), (P=0.088) and (P=0.060) respectively. Conclusion: This study concluded that storage caused significant difference in Hematocrit, mean cell volume, MCHC, red cell distribution width, Lymphocytes, granulocyte %, and platelets indices (P less than 0.05).
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