Conventional and Rapid Methods for Identification of Staphylococcus aureus from Clinical Specimens
American Journal of Biomedical and Life Sciences
Volume 1, Issue 3, October 2013, Pages: 41-43
Received: Aug. 16, 2013; Published: Sep. 20, 2013
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Nneoma Confidence JeanStephanie Anyanwu, Department of Microbiology, Faculty of Sciences, Ahmadu Bello University, Zaria, Nigeria
Walter Chinaka John, Federal College of Forestry, Jos, Plateau State, Nigeria
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Staphylococcus aureus is a facultative anaerobic Gram-positive coccal bacterium whose incidence ranges from skin, soft tissue, respiratory, bone, joint, endovascular to wound infections. The purpose of this study was to identify Staphylococcus aureus from clinical specimens using routine conventional and rapid tests. Gram staining, catalase test, coagulase test, DNase test, haemolysis on blood agar and Microgen™ STAPH-ID kit tests were carried out. A total of 125 Gram positive cocci were tested. The Gram staining technique yielded 100 (80.00%) Staphylococcus spp (Gram positive cocci in clusters). 89(71.20%) isolates were positive to haemolysis on blood agar. Mannitol Salt Agar, DNase agar and Catalase test correctly identified 69 (55.2%) of the Gram positive cocci to be S. aureus as was confirmed by the Microgen™ STAPH-ID kit test. Coagulase test yielded 66 (52.8%) positive results. The Microgen™ STAPH-ID kit test identified three non-coagulase Staphylococcus aureus isolates. The Microgen™ STAPH-ID kit test was the most reliable of the tests, with accuracy comparable to any other rapid test. However, it is the most expensive of the tests. This study established that conventional tests can be used for direct identification of S. aureus to species level if the battery of tests is increased.
Staphylococcus aureus, Conventional Tests, Rapid Tests, Coagulase Test, DNase Test, Microgen™ STAPH-ID
To cite this article
Nneoma Confidence JeanStephanie Anyanwu, Walter Chinaka John, Conventional and Rapid Methods for Identification of Staphylococcus aureus from Clinical Specimens, American Journal of Biomedical and Life Sciences. Vol. 1, No. 3, 2013, pp. 41-43. doi: 10.11648/j.ajbls.20130103.11
F. Menichetti, "Current and Emerging Serious Gram-Positive Infections", Clinical Microbiology and Infections, vol. 11(3), pp. 22–28, 2005, doi:10.1111/j.1469-0691.2005.01138.x. PMID 15811021.
D. T Durack, A. S Lukes, D. K Bright, S. Duke, New criteria for diagnosis of infective endocarditis: utilization of specific echocardiographic findings, Am J Med, vol.96(3), pp. 200-209, 1994.
F. Martineau, F. J. Picard, P. H. Roy, M. Ouellette, M. G. Bergeron, Species-specific and ubiquitous-DNA-based assays for rapid identification of Staphylococcus aureus, J Clin Microbiol, vol. 36(3), pp. 618-623, 1998.
C. S. S Bello, A. Qahtani, Pitfalls in the routine diagnosis of Staphylococcus aureus, Afr J Biotech, vol. 4(1), pp. 83-86, 2006.
J.N.M. Mugalu, S. Kiguli, D. H Kaddu-Mulindwa, Aetiology, risk factors and immediate outcome of bacteriologically confirmed neonatal septicaemia in Mulago hospital, Uganda, Afr Health Sc, vol. 6(2), pp. 120-126, 2006.
W. E. Kloos, T. L. Bannerman, Staphylococcus and Micrococcus, In: Manual of Clinical Microbiology, 6th edn,. Edited by P. R. Murr, pp. 282–298, 1995.
G. H. Chapman, The significance of sodium chloride in studies of staphylococci, J Bacteriol, vol. 50, pp. 201–203, 1945.
J. P. Duguid, Staphylococcus: cluster-forming Gram-positive cocci. In: Mackie and McCartney Practical Medical Microbiology, 13th edn, Edited by J. G. Colle, J. P. Duguid, A. G. Fraser & B. P. Marmion. New York: Churchill Livingstone, pp. 303–316, 1989.
P. Jayaratne, C. Rutherford, Detection of methicillin-resistant Staphylococcus aureus (MRSA) from growth on mannitol salt oxacillin agar using PCR for nosocomial surveillance, Diagn Microbiol Infect Dis, vol. 35, pp. 13–18, 1999.
A. Simor, J. Goodfellow, L. Loiue, M. Loiue, Evaluation of a new medium, oxacillin resistance screening agar base, for the detection of methicillin-resistant Staphylococcus aureus from clinical specimens. J Clin Microbiol, vol. 33, pp. 3422, 2001.
P. M. Zadik, S. Davies, S. Whittaker, C. Mason, Evaluation of a new selective medium for methicillin-resistant Staphylococcus aureus, J Med Microbiol, vol. 50, pp. 476–479, 2001.
F. C. Najjuka, D. P. Kateete, C. N Kimani, F. A. Katabazi, A. Okeng, M. S Okee, A. Nanteza, M. L Joloba, Identification of Staphylococcus aureus: DNase and Mannitol salt agar improve the efficiency of the tube coagulase test, Annals of Clinical Microbiology and Antimicrobials, vol. 9, pp. 23, 2010, doi:10.1186/1476-0711-9-23
G. W. Procop, N. K. Shrestha, M. J. Tuohy, G. S. Hall, C. M. Isada, Rapid Identification of Staphylococcus aureus and the mecA Gene from BacT/ALERT Blood Culture Bottles by Using the LightCycler System, J Clin Microbiol, vol. 40(7), pp. 2659–2661, 2002, doi: 10.1128/JCM.40.7.2659-2661.2002 PMCID: PMC120611
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