American Journal of Biomedical and Life Sciences

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Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children

Received: 21 October 2013    Accepted:     Published: 20 November 2013
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Abstract

Molecular diagnostic methods offer a rapid and more sensitive strategy with high selectivity compared to the traditional immunoassays and culture techniques. Present study attempted to employ the molecular approach for the diagnosis of respiratory viral- and bacterial infections among the pneumonia infected children in Dhaka Metropolis. Samples were aseptically collected, processed and analyzed by using Multiplex Real Time PCR technique to detect respiratory pathogens within a time frame of February 2013-June 2013. Influenza viruses, human meta-pneumo viruses, respiratory syncytial viruses and other potential infective viruses were diagnosed. Among the infective bacterial population, presence of Mycoplasma pneumoniae, Chlamydia pneumoniae, Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella pneumoniae, Legionella species, Salmonella species were detected. Among the nasopharyngeal and orpharyngeal (NPOP) samples, Influenza B and Streptococcus pneumoniae were found to be dominating (34.8% and 30.4%, respectively) whereas the induced sputum (IS) samples were 100% positive for S. pneumoniae followed by around 50% prevalence with equine arteritis virus, para influenza virus and the respiratory syncytial viruses A and B. Considering the spatial identification of an array of microorganisms, results of the current study sufficiently may endorse such molecular method of diagnosis for the betterment of public health.

DOI 10.11648/j.ajbls.20130103.13
Published in American Journal of Biomedical and Life Sciences (Volume 1, Issue 3, October 2013)
Page(s) 53-57
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Respiratory Pathogens, Pneumonia, Molecular Diagnostic Methods, Public Health

References
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Author Information
  • Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

  • Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

  • Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

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    Kohinoor Akter Raton, Md. Asif Hossain, Rashed Noor. (2013). Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children. American Journal of Biomedical and Life Sciences, 1(3), 53-57. https://doi.org/10.11648/j.ajbls.20130103.13

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    ACS Style

    Kohinoor Akter Raton; Md. Asif Hossain; Rashed Noor. Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children. Am. J. Biomed. Life Sci. 2013, 1(3), 53-57. doi: 10.11648/j.ajbls.20130103.13

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    AMA Style

    Kohinoor Akter Raton, Md. Asif Hossain, Rashed Noor. Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children. Am J Biomed Life Sci. 2013;1(3):53-57. doi: 10.11648/j.ajbls.20130103.13

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  • @article{10.11648/j.ajbls.20130103.13,
      author = {Kohinoor Akter Raton and Md. Asif Hossain and Rashed Noor},
      title = {Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {1},
      number = {3},
      pages = {53-57},
      doi = {10.11648/j.ajbls.20130103.13},
      url = {https://doi.org/10.11648/j.ajbls.20130103.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajbls.20130103.13},
      abstract = {Molecular diagnostic methods offer a rapid and more sensitive strategy with high selectivity compared to the traditional immunoassays and culture techniques. Present study attempted to employ the molecular approach for the diagnosis of respiratory viral- and bacterial infections among the pneumonia infected children in Dhaka Metropolis. Samples were aseptically collected, processed and analyzed by using Multiplex Real Time PCR technique to detect respiratory pathogens within a time frame of February 2013-June 2013. Influenza viruses, human meta-pneumo viruses, respiratory syncytial viruses and other potential infective viruses were diagnosed. Among the infective bacterial population, presence of Mycoplasma pneumoniae, Chlamydia pneumoniae, Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella pneumoniae, Legionella species, Salmonella species were detected. Among the nasopharyngeal and orpharyngeal (NPOP) samples, Influenza B and Streptococcus pneumoniae were found to be dominating (34.8% and 30.4%, respectively) whereas the induced sputum (IS) samples were 100% positive for S. pneumoniae followed by around 50% prevalence with equine arteritis virus, para influenza virus and the respiratory syncytial viruses A and B. Considering the spatial identification of an array of microorganisms, results of the current study sufficiently may endorse such molecular method of diagnosis for the betterment of public health.},
     year = {2013}
    }
    

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    T1  - Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children
    AU  - Kohinoor Akter Raton
    AU  - Md. Asif Hossain
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    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
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    AB  - Molecular diagnostic methods offer a rapid and more sensitive strategy with high selectivity compared to the traditional immunoassays and culture techniques. Present study attempted to employ the molecular approach for the diagnosis of respiratory viral- and bacterial infections among the pneumonia infected children in Dhaka Metropolis. Samples were aseptically collected, processed and analyzed by using Multiplex Real Time PCR technique to detect respiratory pathogens within a time frame of February 2013-June 2013. Influenza viruses, human meta-pneumo viruses, respiratory syncytial viruses and other potential infective viruses were diagnosed. Among the infective bacterial population, presence of Mycoplasma pneumoniae, Chlamydia pneumoniae, Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella pneumoniae, Legionella species, Salmonella species were detected. Among the nasopharyngeal and orpharyngeal (NPOP) samples, Influenza B and Streptococcus pneumoniae were found to be dominating (34.8% and 30.4%, respectively) whereas the induced sputum (IS) samples were 100% positive for S. pneumoniae followed by around 50% prevalence with equine arteritis virus, para influenza virus and the respiratory syncytial viruses A and B. Considering the spatial identification of an array of microorganisms, results of the current study sufficiently may endorse such molecular method of diagnosis for the betterment of public health.
    VL  - 1
    IS  - 3
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