Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children
American Journal of Biomedical and Life Sciences
Volume 1, Issue 3, October 2013, Pages: 53-57
Received: Oct. 21, 2013; Published: Nov. 20, 2013
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Authors
Kohinoor Akter Raton, Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
Md. Asif Hossain, Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
Rashed Noor, Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
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Abstract
Molecular diagnostic methods offer a rapid and more sensitive strategy with high selectivity compared to the traditional immunoassays and culture techniques. Present study attempted to employ the molecular approach for the diagnosis of respiratory viral- and bacterial infections among the pneumonia infected children in Dhaka Metropolis. Samples were aseptically collected, processed and analyzed by using Multiplex Real Time PCR technique to detect respiratory pathogens within a time frame of February 2013-June 2013. Influenza viruses, human meta-pneumo viruses, respiratory syncytial viruses and other potential infective viruses were diagnosed. Among the infective bacterial population, presence of Mycoplasma pneumoniae, Chlamydia pneumoniae, Streptococcus pneumoniae, Staphylococcus aureus, Klebsiella pneumoniae, Legionella species, Salmonella species were detected. Among the nasopharyngeal and orpharyngeal (NPOP) samples, Influenza B and Streptococcus pneumoniae were found to be dominating (34.8% and 30.4%, respectively) whereas the induced sputum (IS) samples were 100% positive for S. pneumoniae followed by around 50% prevalence with equine arteritis virus, para influenza virus and the respiratory syncytial viruses A and B. Considering the spatial identification of an array of microorganisms, results of the current study sufficiently may endorse such molecular method of diagnosis for the betterment of public health.
Keywords
Respiratory Pathogens, Pneumonia, Molecular Diagnostic Methods, Public Health
To cite this article
Kohinoor Akter Raton, Md. Asif Hossain, Rashed Noor, Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children, American Journal of Biomedical and Life Sciences. Vol. 1, No. 3, 2013, pp. 53-57. doi: 10.11648/j.ajbls.20130103.13
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