Hepatoprotective Effect of Parkia Biglobosa Stem Bark Methanolic Extract on Paracetamol Induced Liver Damage in Wistar Rats
American Journal of Biomedical and Life Sciences
Volume 1, Issue 4, December 2013, Pages: 75-78
Received: Oct. 5, 2013;
Published: Dec. 10, 2013
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Meraiyebu Ajibola, Department of Physiology, Bingham University, Karu, Nasarawa, Nigeria
Olaniyan Olugbemi, Department of Physiology, Bingham University, Karu, Nasarawa, Nigeria
Abutu Stephanie, Department of Physiology, Bingham University, Karu, Nasarawa, Nigeria
Dare Joseph, Department of Anatomy, Bingham University, Karu, Nasarawa, Nigeria
Atsukwei Denen, Department of Physiology, Bingham University, Karu, Nasarawa, Nigeria
This study was designed to investigate the effect of the methanolic extract of parkia biglobosa stem bark on a single daily dose of oral administration of 500 mg/kg BW of paracetamol (acetaminophen, PCM) induced hepatotoxicity in wistar rats. The rats were divided into (5 groups. The rats in group I served as control and received distilled water, group II were given orally a single daily dose of 500 mg/kg BW of paracetamol for 7 days. Group III, IV, and V received a single daily dose of 500 mg/kg BW of paracetamol and then treated orally with 140 mg/kg BW acetylcysteine, 100 mg/kg BW low dose and 200 mg/kg BW high dose of parkia biglobosa respectively for 21 days. The activities of liver function marker enzymes were determined in the serum of the rat liver homogenate. Paracetamol caused liver damage as evident by significant increased (p≤0.05) (49.63±1.99; 39.41±1.99; 78.58±1.72) in the serum levels of Alkaline phosphatase (AP), Aspartate transaminase (AST) and Alanine transaminase (ALT) respectively. Low dose 100mg/kg BW of Parkia biglobosa significantly increased (p≤0.05) serum AP levels (65.42±1.6) but significantly reduced serum levels of ALT and AST (43.80±2.4; 36.77±1.58) respectively. High dose 200 mg/kg BW of Parkia biglobosa significantly reduced (p≤0.05) serum levels of AP, ALT and AST (26.58±0.34; 33.68±2.02; 31.08±0.34) respectively. Acetylcysteine (standard reference drug) significantly reduced (p≤0.05) ALT and AST levels (43.46±1.67; 30.10±1.01) respectively, but the reduction in AP level (46.64±1.01) was not significant. The activity of parkia biglobosa is comparable with acetylcysteine, a known hepatoprotective drug. Thus, Parkia biglobosa exhibits hepatoprotective activity against paracetamol toxicity.
Hepatoprotective Effect of Parkia Biglobosa Stem Bark Methanolic Extract on Paracetamol Induced Liver Damage in Wistar Rats, American Journal of Biomedical and Life Sciences.
Vol. 1, No. 4,
2013, pp. 75-78.
Abbiw D.K. (1990). Useful Plants of Ghana. Intermediate Technology Publications and the Royal Botanic Gardens, Kew. UK. p. 337.
Agunu A., Yusuf S., AndrewGO., Zezi AU., Abdurahman EM. (2005). Evaluation of five medicinal plants used in diarrhoea treatment in Nigerian Journal of Ethnopharmacology, 101, 27-30.
Builders M.I., Isichie C.O andAguiyi J. C (2012).Toxicity Studies of the Extracts of Parkia biglobosa Stem Bark in Rats, British Journal of Pharmaceutical Research.2(1): 1-16.
Cushnie TPT, and Lamb AJ (2011). "Recent advances in understanding the antibacterial properties of flavonoids". International Journal of Antimicrobial Agents 38 (2): 99–107.
Daly FF, Fountain JS, Murray L, Graudins A, and Buckley NA (2008). "Guidelines for the management of paracetamol poisoning in Australia and New Zealand—explanation and elaboration. A consensus statement from clinical toxicologists consulting to the Australasian poisons information centres". The Medical Journal of Australia 188 (5): 296–301.
Gini C. Kuriakose Muraleedhara G. and Kurup (2010). Hepatoprotective effect of Spirulinalonar on paracetamol induced liver damage in rats, Asian Journal for Experimental Biological Science, ISSN 0975-5845
Gronhaug T.E., Glaeserud S., Skogsrud M., Ballo N., Bah S., Diallo D., and Paulsen B.S (2008). Ethnopharmacological survey of six medicinal plants from Mali. West Africa Journal of Ethnopharmacology, 4, 4-26.
Halliwell B (1994). Antioxidants Sense or Speculation. Nutrition Today, 29(6):15-19.
Klein B., Read P.A. and Babson L.A., 1960. Rapid method for the quantitative determination of serum alkaline phosphatase. Clinical Chemistry, 6: 269-275.
Kumar G, Sharmila BG, Kannan V and Rajasekara PM (2005). Antihepatotoxic effect of Beta-carotene on paracetamol induced hepatic damage in rats. Indian Journal of Experimental Biology, 43(4): 351-355.
Larson AM, Polson J, Fontana RJ, Davern TJ, Lalani E, Hynan LS, Reisch JS, Schiødt FV, Ostapowicz G, Shakil AO, and Lee WM (2005). Acute Liver Failure Study Group."Acetaminophen-induced acute liver failure: results of a United States multicenter, prospective study". Hepatology 42 (6): 1364-1372.
Millogo-Kone H, Guisson IP, Nacoulna O, and Traore AS (2006). Study of the antibacterial activity of the stem bark and leaf extracts of Parkia biglobosa (Jacq) Benth on Staphylococcus aureus. African Journal of Traditional Comparative Alternative Medicine. 3(2): 74- 78.
Benjamin L. Shneider; Sherman, Philip M. (2008). Pediatric Gastrointestinal Disease. Connecticut: PMPH-USA. pp. 751. ISBN 1-55009-364-9.
Pieme C.A., Penlap V.N., Nkegoum B.,Taziebou C.L., Tekwu E.M., Etoa F.X., and Ngongang J. (2006):Evaluation of acute and subacute toxicities of aqueous ethanolic extract of leaves of Sennaalata (L) Roxb (Ceasalpiniaceae). African Journal of Biotechnology, 5, 283-289.
Reitman S, and Frankel S. (1957) A colorimetric method for determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology 28: 56-63
Tijani A.Y.,Okhale S.E., Salawu T.A., Onigbanjo H.O., Obianodo L.A., AkingbasoteJ.A. Salawu O.A., Okogun J.E., Kunle F.O., and Emeje M. (2009). Anti diarrhealand antibacterial properties of crude aqueous stem bark extract and fractions of P. biglobosa (Jacq) R.Br Ex G. Don. African Journal of Pharmacy and Pharmacology, 7,347-353.
Gronhaug, T.E., Glaeserud, S., Skogsrud, M., Ballo, N., Bah, S., Diallo, D., Paulsen, B.S (2008). Ethnopharmacological survey of six medicinal plants from Mali. West Africa Journal of Ethnopharmacology, 4, 4-26.
Tijani, A.Y., Okhale, S.E., Salawu, T.A., Onigbanjo, H.O., Obianodo, L.A., Akingbasote, J.A., Salawu, O.A., Okogun, J.E., Kunle, F.O., Emeje, M. (2009). Anti diarrheal and antibacterial properties of crude aqueous stem bark extract and fractions of P. biglobosa (Jacq) R.Br Ex G. Don. African Journal of Pharmacy and Pharmacology, 7,347-353.
Navarro, C. Montilla, P Martin, A Jimenez J and Utrilla. P (1993). Plant Medicine,, 59, 312-31420 Ragavan, B and Krishnakumari, S (2006) Indian journal of clinical Biochemistry., 21,123-128.
Baraa N. AL-Okaily, Rawya Shaker Mohammed, Kahtan A. Al-Mzain and Khalisa Khadim Khudair (2012) Effect of Flavonoids Extracted from Black Cumin (Nigella sativa) and Vitamin E in Ameliorating Hepatic Damage Induced by Sodium Nitrate in adult male rats. Proceeding of the Eleventh Veterinary Scientific Conference, 172-181