Chemical Composition, Antibacterial and Antifungal Activity of the Essential oil of Pinus Patula Growing in Rwanda

Tomani Jean Claude; Murangwa Christine; Bajyana Songa; Mukazayire Marie Jeanne; Ingabire Mukazi Goretti; Chalchat Jean Claude

doi:doi:10.11648/j.ajbls.20140203.11

Chemical Composition, Antibacterial and Antifungal Activity of the Essential oil of Pinus Patula Growing in Rwanda

Tomani Jean Claude, Murangwa Christine, Bajyana Songa, Mukazayire Marie Jeanne, Ingabire Mukazi Goretti, Chalchat Jean Claude

Published in American Journal of Biomedical and Life Sciences (Volume 2, Issue 3)

Received: 12 May 2014 Accepted: 5 June 2014 Published: 20 June 2014

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Abstract

Essential oils and their components are increasingly spreading as naturally occurring antimicrobial agents. In this work the chemical composition and the antimicrobial properties of Pinus patula essential oils, a wild Pinaceae, which grows in several regions of Rwanda, were characterized and assessed. The essential oil was obtained by steam distillation and the chemical composition was determined using GC/MS. The in vitro antimicrobial activity of the essential oil was studied against two Gram-negative bacteria (Streptococcus pyogenis, Pseudomonas solanacearum) and one Gram-positive bacteria (Staphylococcus aureus) and two fungi (Pycularia oryzae, Colletotrichum coffeanum) using a broth macrodilution method and by agar diffusion method. During the chemical composition analysis, seventy-four components making up 99.11% of the oil were detected while only fifty components making up 95.70% of the oil were characterized, β-phellandrene (18.98%), α-pinene (15.91%), bornyl acetate (7.89%), β-caryophyllene (7.41%), limonene (5.67%) being the major constituents. The results of the in vitro antimicrobial assay showed that essential oil extracted from the rwandese Pinus patula has a strong activity against all tested bacteria and fungi, exception done to Colletotrichum coffeanum fungi.

Published in	American Journal of Biomedical and Life Sciences (Volume 2, Issue 3)
DOI	10.11648/j.ajbls.20140203.11
Page(s)	55-59
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Essential Oil, Pinus Patula, Rwanda, Chemical Composition, Antimicrobial Activity

References

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APA Style

Tomani Jean Claude, Murangwa Christine, Bajyana Songa, Mukazayire Marie Jeanne, Ingabire Mukazi Goretti, et al. (2014). Chemical Composition, Antibacterial and Antifungal Activity of the Essential oil of Pinus Patula Growing in Rwanda. American Journal of Biomedical and Life Sciences, 2(3), 55-59. https://doi.org/10.11648/j.ajbls.20140203.11

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ACS Style

Tomani Jean Claude; Murangwa Christine; Bajyana Songa; Mukazayire Marie Jeanne; Ingabire Mukazi Goretti, et al. Chemical Composition, Antibacterial and Antifungal Activity of the Essential oil of Pinus Patula Growing in Rwanda. Am. J. Biomed. Life Sci. 2014, 2(3), 55-59. doi: 10.11648/j.ajbls.20140203.11

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AMA Style

Tomani Jean Claude, Murangwa Christine, Bajyana Songa, Mukazayire Marie Jeanne, Ingabire Mukazi Goretti, et al. Chemical Composition, Antibacterial and Antifungal Activity of the Essential oil of Pinus Patula Growing in Rwanda. Am J Biomed Life Sci. 2014;2(3):55-59. doi: 10.11648/j.ajbls.20140203.11

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@article{10.11648/j.ajbls.20140203.11,
  author = {Tomani Jean Claude and Murangwa Christine and Bajyana Songa and Mukazayire Marie Jeanne and Ingabire Mukazi Goretti and Chalchat Jean Claude},
  title = {Chemical Composition, Antibacterial and Antifungal Activity of the Essential oil of Pinus Patula Growing in Rwanda},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {2},
  number = {3},
  pages = {55-59},
  doi = {10.11648/j.ajbls.20140203.11},
  url = {https://doi.org/10.11648/j.ajbls.20140203.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20140203.11},
  abstract = {Essential oils and their components are increasingly spreading as naturally occurring antimicrobial agents. In this work the chemical composition and the antimicrobial properties of Pinus patula essential oils, a wild Pinaceae, which grows in several regions of Rwanda, were characterized and assessed. The essential oil was obtained by steam distillation and the chemical composition was determined using GC/MS. The in vitro antimicrobial activity of the essential oil was studied against two Gram-negative bacteria (Streptococcus pyogenis, Pseudomonas solanacearum) and one Gram-positive bacteria (Staphylococcus aureus) and two fungi (Pycularia oryzae, Colletotrichum coffeanum) using a broth macrodilution method and by agar diffusion method. During the chemical composition analysis, seventy-four components making up 99.11% of the oil were detected while only fifty components making up 95.70% of the oil were characterized,  β-phellandrene (18.98%), α-pinene (15.91%), bornyl acetate (7.89%), β-caryophyllene (7.41%), limonene (5.67%) being the major constituents. The results of the in vitro antimicrobial assay showed that essential oil extracted from the rwandese Pinus patula has a strong activity against all tested bacteria and fungi, exception done to Colletotrichum coffeanum fungi.},
 year = {2014}
}

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TY  - JOUR
T1  - Chemical Composition, Antibacterial and Antifungal Activity of the Essential oil of Pinus Patula Growing in Rwanda
AU  - Tomani Jean Claude
AU  - Murangwa Christine
AU  - Bajyana Songa
AU  - Mukazayire Marie Jeanne
AU  - Ingabire Mukazi Goretti
AU  - Chalchat Jean Claude
Y1  - 2014/06/20
PY  - 2014
N1  - https://doi.org/10.11648/j.ajbls.20140203.11
DO  - 10.11648/j.ajbls.20140203.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 55
EP  - 59
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20140203.11
AB  - Essential oils and their components are increasingly spreading as naturally occurring antimicrobial agents. In this work the chemical composition and the antimicrobial properties of Pinus patula essential oils, a wild Pinaceae, which grows in several regions of Rwanda, were characterized and assessed. The essential oil was obtained by steam distillation and the chemical composition was determined using GC/MS. The in vitro antimicrobial activity of the essential oil was studied against two Gram-negative bacteria (Streptococcus pyogenis, Pseudomonas solanacearum) and one Gram-positive bacteria (Staphylococcus aureus) and two fungi (Pycularia oryzae, Colletotrichum coffeanum) using a broth macrodilution method and by agar diffusion method. During the chemical composition analysis, seventy-four components making up 99.11% of the oil were detected while only fifty components making up 95.70% of the oil were characterized,  β-phellandrene (18.98%), α-pinene (15.91%), bornyl acetate (7.89%), β-caryophyllene (7.41%), limonene (5.67%) being the major constituents. The results of the in vitro antimicrobial assay showed that essential oil extracted from the rwandese Pinus patula has a strong activity against all tested bacteria and fungi, exception done to Colletotrichum coffeanum fungi.
VL  - 2
IS  - 3
ER  -

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Author Information

Tomani Jean Claude

Research Program in Phytomedecine and Life Science, Institute of Scientific and Technological Research, Huye, Rwanda
Murangwa Christine

Department of Biotechnology, Faculty of Science, National University of Rwanda, Huye, Rwanda
Bajyana Songa

Department of Biotechnology, Faculty of Science, National University of Rwanda, Huye, Rwanda
Mukazayire Marie Jeanne

Research Program in Phytomedecine and Life Science, Institute of Scientific and Technological Research, Huye, Rwanda
Ingabire Mukazi Goretti

Research Program in Phytomedecine and Life Science, Institute of Scientific and Technological Research, Huye, Rwanda
Chalchat Jean Claude

Laboratory of Photochemistry Molecular and Macromolecular, Chemistry of Essential Oils, Blaise Pascal Clermont University, Aubière Cédex, France

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APA Style

Tomani Jean Claude, Murangwa Christine, Bajyana Songa, Mukazayire Marie Jeanne, Ingabire Mukazi Goretti, et al. (2014). Chemical Composition, Antibacterial and Antifungal Activity of the Essential oil of Pinus Patula Growing in Rwanda. American Journal of Biomedical and Life Sciences, 2(3), 55-59. https://doi.org/10.11648/j.ajbls.20140203.11

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ACS Style

Tomani Jean Claude; Murangwa Christine; Bajyana Songa; Mukazayire Marie Jeanne; Ingabire Mukazi Goretti, et al. Chemical Composition, Antibacterial and Antifungal Activity of the Essential oil of Pinus Patula Growing in Rwanda. Am. J. Biomed. Life Sci. 2014, 2(3), 55-59. doi: 10.11648/j.ajbls.20140203.11

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AMA Style

Tomani Jean Claude, Murangwa Christine, Bajyana Songa, Mukazayire Marie Jeanne, Ingabire Mukazi Goretti, et al. Chemical Composition, Antibacterial and Antifungal Activity of the Essential oil of Pinus Patula Growing in Rwanda. Am J Biomed Life Sci. 2014;2(3):55-59. doi: 10.11648/j.ajbls.20140203.11

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@article{10.11648/j.ajbls.20140203.11,
  author = {Tomani Jean Claude and Murangwa Christine and Bajyana Songa and Mukazayire Marie Jeanne and Ingabire Mukazi Goretti and Chalchat Jean Claude},
  title = {Chemical Composition, Antibacterial and Antifungal Activity of the Essential oil of Pinus Patula Growing in Rwanda},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {2},
  number = {3},
  pages = {55-59},
  doi = {10.11648/j.ajbls.20140203.11},
  url = {https://doi.org/10.11648/j.ajbls.20140203.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20140203.11},
  abstract = {Essential oils and their components are increasingly spreading as naturally occurring antimicrobial agents. In this work the chemical composition and the antimicrobial properties of Pinus patula essential oils, a wild Pinaceae, which grows in several regions of Rwanda, were characterized and assessed. The essential oil was obtained by steam distillation and the chemical composition was determined using GC/MS. The in vitro antimicrobial activity of the essential oil was studied against two Gram-negative bacteria (Streptococcus pyogenis, Pseudomonas solanacearum) and one Gram-positive bacteria (Staphylococcus aureus) and two fungi (Pycularia oryzae, Colletotrichum coffeanum) using a broth macrodilution method and by agar diffusion method. During the chemical composition analysis, seventy-four components making up 99.11% of the oil were detected while only fifty components making up 95.70% of the oil were characterized,  β-phellandrene (18.98%), α-pinene (15.91%), bornyl acetate (7.89%), β-caryophyllene (7.41%), limonene (5.67%) being the major constituents. The results of the in vitro antimicrobial assay showed that essential oil extracted from the rwandese Pinus patula has a strong activity against all tested bacteria and fungi, exception done to Colletotrichum coffeanum fungi.},
 year = {2014}
}

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TY  - JOUR
T1  - Chemical Composition, Antibacterial and Antifungal Activity of the Essential oil of Pinus Patula Growing in Rwanda
AU  - Tomani Jean Claude
AU  - Murangwa Christine
AU  - Bajyana Songa
AU  - Mukazayire Marie Jeanne
AU  - Ingabire Mukazi Goretti
AU  - Chalchat Jean Claude
Y1  - 2014/06/20
PY  - 2014
N1  - https://doi.org/10.11648/j.ajbls.20140203.11
DO  - 10.11648/j.ajbls.20140203.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 55
EP  - 59
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20140203.11
AB  - Essential oils and their components are increasingly spreading as naturally occurring antimicrobial agents. In this work the chemical composition and the antimicrobial properties of Pinus patula essential oils, a wild Pinaceae, which grows in several regions of Rwanda, were characterized and assessed. The essential oil was obtained by steam distillation and the chemical composition was determined using GC/MS. The in vitro antimicrobial activity of the essential oil was studied against two Gram-negative bacteria (Streptococcus pyogenis, Pseudomonas solanacearum) and one Gram-positive bacteria (Staphylococcus aureus) and two fungi (Pycularia oryzae, Colletotrichum coffeanum) using a broth macrodilution method and by agar diffusion method. During the chemical composition analysis, seventy-four components making up 99.11% of the oil were detected while only fifty components making up 95.70% of the oil were characterized,  β-phellandrene (18.98%), α-pinene (15.91%), bornyl acetate (7.89%), β-caryophyllene (7.41%), limonene (5.67%) being the major constituents. The results of the in vitro antimicrobial assay showed that essential oil extracted from the rwandese Pinus patula has a strong activity against all tested bacteria and fungi, exception done to Colletotrichum coffeanum fungi.
VL  - 2
IS  - 3
ER  -

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