American Journal of Biomedical and Life Sciences
Volume 2, Issue 4, August 2014, Pages: 65-69
Received: Jun. 13, 2014;
Accepted: Jul. 7, 2014;
Published: Jul. 30, 2014
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Seidu Mahmood Abdulai, Department of Medical laboratory science, School of Allied Health Sciences, University of Ghana, Accra, Ghana
Adams Abdul Rashid, Department of Medical laboratory science, School of Allied Health Sciences, University of Ghana, Accra, Ghana
Gyasi Kwame Richard, Department of Pathology, University of Ghana Medical School, Accra, Ghana
Tettey Yao, Department of Pathology, University of Ghana Medical School, Accra, Ghana
Nkansah Obenewaa Dinah, Department of Medical laboratory science, School of Allied Health Sciences, University of Ghana, Accra, Ghana
Adunyame Lois, Department of Medical laboratory science, School of Allied Health Sciences, University of Ghana, Accra, Ghana
Wiredu Kwame Edwin, Department of Medical laboratory science, School of Allied Health Sciences, University of Ghana, Accra, Ghana
Onchocerca volvulus is a parasite responsible for the disease, Onchocerciasis otherwise known as river blindness. Current treatment and control strategies are not entirely successful. This means there is a need for further studies to better understand the biology of this worm. Studies on metabolic pathways in the worm can provide valuable information to deepen our understanding of the mechanism of survival of the worm. Lipid catabolism for the provision of energy in this worm still remains to be elucidated. Knowledge of lipids composition and synthesis in this parasite has been mostly drawn from studies on filarial species other than O. volvulus itself. This is owed to the limited availability of parasite material due largely to problems with parasite cultivation and ethical demands on obtaining materials since it has a strict preference for the human host. To explore lipid catabolism in the worm itself, we performed immunohistochemical localisation of three major enzymes (A/B hydrolase, SDH and ME1) involved in lipid metabolism on paraffin processed archival O. volvulus nodules. We observed that up to 58.6% of worms in the paraffin processed nodules had detectable A/B/HD4, 51.6% had SDH and 63.3% had ME 1, most of which were stored in the muscles of the adult worm. These observations suggest that the adult O. volvulus can operate the B-oxidative pathway, The TCA cycle, and undergo anaplerotic transformation of malic acid to pyruvate to maximise energy production from lipid metabolism. This also suggests that O. volvulus has the capacity to catabolise lipids for energy even though it appears to be dependent on carbohydrate metabolism.
Seidu Mahmood Abdulai,
Adams Abdul Rashid,
Gyasi Kwame Richard,
Nkansah Obenewaa Dinah,
Wiredu Kwame Edwin,
Immunohistochemical Study on lipid Catabolism in Adult Onchocerca Volvulus, American Journal of Biomedical and Life Sciences.
Vol. 2, No. 4,
2014, pp. 65-69.
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