Background. Several studies have identified a protein of OMP S. Typhi. The protein is highly immunogenic and can be recognize whole cells of S. Typhi. The aims of the study was to evaluate the Dri-dot OMPs immunoreactivity in typhoid fever suspected patients. Method. Samples obtained from Dr. Wahidin Sudirohusodo Hospital, lbnu Sina Hospital, and Haji Hospital of Makassar from February to November 2014. Their sensitivity and specificity were evaluated against blood culture method as the gold standard. For each of the two tests, sensitivity, specificity, positive and negative predictive values were calculated using standard formulae. Results. A total of 15 of suspected typhoid fever samples examined for culture, lateral flow and Dri-dot OMPs. Examination of blood culture method showed that 3 of 15 (20%) patients had a blood culture positive for S. typhi. Dri-dot OMPs were positive in 13 (86.7%) serum samples. Fourteen (93.3%) serum samples were positive for lateral flow examination. Thirteen samples were positive for both Dri-dot and lateral flow. One sample was negative for both lateral flow and Dri-dot. One sample was positive for Dri-dot but negative for lateral flow. Conclusion. We conclude that there was no difference between Dri-dot OMPs, lateral flow and widal tests. Dri-dot could be of use for the diagnosis of typhoid fever in patients who have clinical typhoid fever
| Published in | American Journal of Biomedical and Life Sciences (Volume 3, Issue 4) |
| DOI | 10.11648/j.ajbls.20150304.14 |
| Page(s) | 87-90 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Salmonella Typhi, Typhoid Fever, Lateral Flow, Dri-Dot OMPs
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APA Style
Yusriani Mangarengi, Ressy Dwiyanti, Nataniel Tandirogang, Muhammad Sabir, Rosdiana Natzir, et al. (2015). Evaluation of Dri-Dot OMPs Salmonella Typhi in Suspected Typhoid Fever Patients as an Immunodiagnostic Kit. American Journal of Biomedical and Life Sciences, 3(4), 87-90. https://doi.org/10.11648/j.ajbls.20150304.14
ACS Style
Yusriani Mangarengi; Ressy Dwiyanti; Nataniel Tandirogang; Muhammad Sabir; Rosdiana Natzir, et al. Evaluation of Dri-Dot OMPs Salmonella Typhi in Suspected Typhoid Fever Patients as an Immunodiagnostic Kit. Am. J. Biomed. Life Sci. 2015, 3(4), 87-90. doi: 10.11648/j.ajbls.20150304.14
AMA Style
Yusriani Mangarengi, Ressy Dwiyanti, Nataniel Tandirogang, Muhammad Sabir, Rosdiana Natzir, et al. Evaluation of Dri-Dot OMPs Salmonella Typhi in Suspected Typhoid Fever Patients as an Immunodiagnostic Kit. Am J Biomed Life Sci. 2015;3(4):87-90. doi: 10.11648/j.ajbls.20150304.14
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Download@article{10.11648/j.ajbls.20150304.14,
author = {Yusriani Mangarengi and Ressy Dwiyanti and Nataniel Tandirogang and Muhammad Sabir and Rosdiana Natzir and Mochammad Hatta and Yadi},
title = {Evaluation of Dri-Dot OMPs Salmonella Typhi in Suspected Typhoid Fever Patients as an Immunodiagnostic Kit},
journal = {American Journal of Biomedical and Life Sciences},
volume = {3},
number = {4},
pages = {87-90},
doi = {10.11648/j.ajbls.20150304.14},
url = {https://doi.org/10.11648/j.ajbls.20150304.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20150304.14},
abstract = {Background. Several studies have identified a protein of OMP S. Typhi. The protein is highly immunogenic and can be recognize whole cells of S. Typhi. The aims of the study was to evaluate the Dri-dot OMPs immunoreactivity in typhoid fever suspected patients. Method. Samples obtained from Dr. Wahidin Sudirohusodo Hospital, lbnu Sina Hospital, and Haji Hospital of Makassar from February to November 2014. Their sensitivity and specificity were evaluated against blood culture method as the gold standard. For each of the two tests, sensitivity, specificity, positive and negative predictive values were calculated using standard formulae. Results. A total of 15 of suspected typhoid fever samples examined for culture, lateral flow and Dri-dot OMPs. Examination of blood culture method showed that 3 of 15 (20%) patients had a blood culture positive for S. typhi. Dri-dot OMPs were positive in 13 (86.7%) serum samples. Fourteen (93.3%) serum samples were positive for lateral flow examination. Thirteen samples were positive for both Dri-dot and lateral flow. One sample was negative for both lateral flow and Dri-dot. One sample was positive for Dri-dot but negative for lateral flow. Conclusion. We conclude that there was no difference between Dri-dot OMPs, lateral flow and widal tests. Dri-dot could be of use for the diagnosis of typhoid fever in patients who have clinical typhoid fever},
year = {2015}
}
TY - JOUR T1 - Evaluation of Dri-Dot OMPs Salmonella Typhi in Suspected Typhoid Fever Patients as an Immunodiagnostic Kit AU - Yusriani Mangarengi AU - Ressy Dwiyanti AU - Nataniel Tandirogang AU - Muhammad Sabir AU - Rosdiana Natzir AU - Mochammad Hatta AU - Yadi Y1 - 2015/08/13 PY - 2015 N1 - https://doi.org/10.11648/j.ajbls.20150304.14 DO - 10.11648/j.ajbls.20150304.14 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 87 EP - 90 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20150304.14 AB - Background. Several studies have identified a protein of OMP S. Typhi. The protein is highly immunogenic and can be recognize whole cells of S. Typhi. The aims of the study was to evaluate the Dri-dot OMPs immunoreactivity in typhoid fever suspected patients. Method. Samples obtained from Dr. Wahidin Sudirohusodo Hospital, lbnu Sina Hospital, and Haji Hospital of Makassar from February to November 2014. Their sensitivity and specificity were evaluated against blood culture method as the gold standard. For each of the two tests, sensitivity, specificity, positive and negative predictive values were calculated using standard formulae. Results. A total of 15 of suspected typhoid fever samples examined for culture, lateral flow and Dri-dot OMPs. Examination of blood culture method showed that 3 of 15 (20%) patients had a blood culture positive for S. typhi. Dri-dot OMPs were positive in 13 (86.7%) serum samples. Fourteen (93.3%) serum samples were positive for lateral flow examination. Thirteen samples were positive for both Dri-dot and lateral flow. One sample was negative for both lateral flow and Dri-dot. One sample was positive for Dri-dot but negative for lateral flow. Conclusion. We conclude that there was no difference between Dri-dot OMPs, lateral flow and widal tests. Dri-dot could be of use for the diagnosis of typhoid fever in patients who have clinical typhoid fever VL - 3 IS - 4 ER -
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