Evaluation of Dri-Dot OMPs Salmonella Typhi in Suspected Typhoid Fever Patients as an Immunodiagnostic Kit
American Journal of Biomedical and Life Sciences
Volume 3, Issue 4, August 2015, Pages: 87-90
Received: Jul. 17, 2015; Accepted: Jul. 29, 2015; Published: Aug. 13, 2015
Views 3412      Downloads 66
Authors
Yusriani Mangarengi, Department of Microbiology, Faculty of Medicine, Muslim University of Indonesia, Makassar, Indonesia; Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Ressy Dwiyanti, Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Nataniel Tandirogang, Department Microbiology and Immunology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia
Muhammad Sabir, Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Rosdiana Natzir, Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Mochammad Hatta, Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Yadi , Department Microbiology and Immunology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia
Article Tools
Follow on us
Abstract
Background. Several studies have identified a protein of OMP S. Typhi. The protein is highly immunogenic and can be recognize whole cells of S. Typhi. The aims of the study was to evaluate the Dri-dot OMPs immunoreactivity in typhoid fever suspected patients. Method. Samples obtained from Dr. Wahidin Sudirohusodo Hospital, lbnu Sina Hospital, and Haji Hospital of Makassar from February to November 2014. Their sensitivity and specificity were evaluated against blood culture method as the gold standard. For each of the two tests, sensitivity, specificity, positive and negative predictive values were calculated using standard formulae. Results. A total of 15 of suspected typhoid fever samples examined for culture, lateral flow and Dri-dot OMPs. Examination of blood culture method showed that 3 of 15 (20%) patients had a blood culture positive for S. typhi. Dri-dot OMPs were positive in 13 (86.7%) serum samples. Fourteen (93.3%) serum samples were positive for lateral flow examination. Thirteen samples were positive for both Dri-dot and lateral flow. One sample was negative for both lateral flow and Dri-dot. One sample was positive for Dri-dot but negative for lateral flow. Conclusion. We conclude that there was no difference between Dri-dot OMPs, lateral flow and widal tests. Dri-dot could be of use for the diagnosis of typhoid fever in patients who have clinical typhoid fever
Keywords
Salmonella Typhi, Typhoid Fever, Lateral Flow, Dri-Dot OMPs
To cite this article
Yusriani Mangarengi, Ressy Dwiyanti, Nataniel Tandirogang, Muhammad Sabir, Rosdiana Natzir, Mochammad Hatta, Yadi , Evaluation of Dri-Dot OMPs Salmonella Typhi in Suspected Typhoid Fever Patients as an Immunodiagnostic Kit, American Journal of Biomedical and Life Sciences. Vol. 3, No. 4, 2015, pp. 87-90. doi: 10.11648/j.ajbls.20150304.14
References
[1]
Baker S, Favorov M, Dougan G: Searching for the elusive typhoid diagnostic. BioMed Central Infectious Diseases 2010, 10(45).
[2]
Yang HH, Kilgore PE, Yang LH, Park JK, Pan YF, Kim Y, Lee YJ, Xu ZY, Clemens JD: An outbreak of typhoid fever, Xing-An County, People's Republic of China, 1999: estimation of the field effectiveness of Vi polysaccharide typhoid vaccine. The Journal of infectious diseases 2001, 183(12):1775-1780.
[3]
Santiviago CA, Toro CS, Bucarey SA, Mora GC: A chromosomal region surrounding the ompD porin gene marks a genetic difference between Salmonella typhi and the majority of Salmonella serovars. Microbiology 2001, 147(Pt 7):1897-1907.
[4]
Zhou L, & Pollard: A fast and highly sensitive blood culture PCR method for clinical detection of Salmonella enterica serovar Typhi Annals of Clinical Microbiology and Antimicrobials 2010, 9( 14).
[5]
Parry CM: Epidemiological and clinical aspects of human. In: Salmonella infections clinical, immunological and molecular aspects edn. Edited by P.Mastroeni DM. Cambridge: Cambridge University Press. ; 2006: 1-17.
[6]
Olsen SJ, Pruckler J, Bibb W, Thanh NT, Trinh TM, Minh NT: Evaluation of rapid diagnostic tests for typhoid fever. Journal of clinical microbiology 2004, 42(5):1885–1889. .
[7]
Parry CM, Hoa NT, Diep TS, Wain J, Chinh NT, Vinh H, Hien TT, White NJ, Farrar JJ: Value of a single-tube widal test in diagnosis of typhoid fever in Vietnam. Journal of clinical microbiology 1999, 37(9):2882-2886.
[8]
Fadeel MA, House BL, Wasfy MM, Klena JD, Habashy EE, Said MM, Maksoud MA, Rahman BA, Pimentel G: Evaluation of a newly developed ELISA against Widal, TUBEX-TF and Typhidot for typhoid fever surveillance. Journal of infection in developing countries 2011, 5(3):169-175.
[9]
Isibasi A, Ortiz V, Vargas M, Paniagua J, Gonzalez C, Moreno J, Kumate J: Protection against Salmonella typhi infection in mice after immunization with outer membrane proteins isolated from Salmonella typhi 9,12,d, Vi. Infection and immunity 1988, 56(11):2953-2959.
[10]
Winarsih S: Cloning and expression gene Adhesin Adho36 of S.Typhi as candidate vaccines of Typhoid fever. . In: Report of Research RISTEK. 2007.
[11]
MacConkey: Note on a new medium for the growth and differentiation of the bacillus coli communis and the bacillus typhi abdominalis. The Lancet 1900, 156(4010):172.
[12]
CDC., WHO.: Manual for the Laboratory Identification and Antimicrobial Susceptibility Testing of Bacterial Pathogens of Public Health Importance in the Developing World. In.; (2003). .
[13]
Tarupiwa A, Tapera S, Mtapuri-Zinyowera S, Gumbo P, Ruhanya V, Gudza-Mugabe M, Majuru NX, Chin'ombe N: Evaluation of TUBEX-TF and OnSite Typhoid IgG/IgM Combo rapid tests to detect Salmonella enterica serovar Typhi infection during a typhoid outbreak in Harare, Zimbabwe. BMC research notes 2015, 8:50.
[14]
Arjunan M, Al-Salamah AA: Typhoid fever with severe abdominal pain: diagnosis and clinical findings using abdomen ultrasonogram, hematology-cell analysis and the Widal test. Journal of infection in developing countries 2010, 4(9):593-596.
[15]
Sheikh A, Bhuiyan MS, Khanam F, Chowdhury F, Saha A, Ahmed D, Jamil KM, LaRocque RC, Harris JB, Ahmad MM et al: Salmonella enterica serovar Typhi-specific immunoglobulin A antibody responses in plasma and antibody in lymphocyte supernatant specimens in Bangladeshi patients with suspected typhoid fever. Clinical and vaccine immunology : CVI 2009, 16(11):1587-1594.
[16]
16. Song JH, Cho H, Park MY, Na DS, Moon HB, Pai CH: Detection of Salmonella typhi in the blood of patients with typhoid fever by polymerase chain reaction. Journal of clinical microbiology 1993, 31(6):1439-1443.
[17]
Hansen KR, Nielsen LR, Lind P: Use of IgG avidity ELISA to differentiate acute from persistent infection with Salmonella Dublin in cattle. Journal of applied microbiology 2006, 100(1):144-152.
[18]
Smith SI, Bamidele M, Fowora M, Goodluck HT, Omonigbehin EA, Akinsinde KA, Fesobi T, Pastoor R, Abdoel TH, Smits HL: Application of a point-of-care test for the serodiagnosis of typhoid fever in Nigeria and the need for improved diagnostics. Journal of infection in developing countries 2011, 5(7):520-526.
[19]
Mahbubur Rahman, Siddique AK, Tam FC-H, Sabrina Sharmin, Harunur Rashid, Anwarul Iqbal, Sirajuddin Ahmed, Gopinath Balkrish Nair, Claire-Lise Chaignat, Lim P-L: Rapid detection of early typhoid fever in endemic community children by the TUBEX O9-antibody test. Diagnostic microbiology and infectious disease 2007.
[20]
House D, Wain J, Ho VA, Diep TS, Chinh NT, Bay PV, Vinh H, Duc M, Parry CM, Dougan G et al: Serology of typhoid fever in an area of endemicity and its relevance to diagnosis. Journal of clinical microbiology 2001, 39(3):1002-1007.
[21]
Safari Foroshani N, Karami A, Pourali F: Simultaneous and Rapid Detection of Salmonella typhi, Bacillus anthracis, and Yersinia pestis by Using Multiplex Polymerase Chain Reaction (PCR). Iranian Red Crescent medical journal 2013, 15(11):e9208.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186