Paraoxonase-1 and Glutathione Peroxidase Activity as a Screening Tool in Detecting Severity of Coronary Artery Disease: Case Control Feasibility Study
American Journal of Biomedical and Life Sciences
Volume 3, Issue 6, December 2015, Pages: 120-126
Received: Dec. 13, 2015; Accepted: Dec. 23, 2015; Published: Jan. 4, 2016
Views 3456      Downloads 107
Authors
Dinushka Wickramasinghe, Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
Hemantha Peiris, Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
Lal Gotabhaya Chandrasena, Nawaloka Hospitals, Colombo, Sri Lanka
Vajira Senaratne, Cardiology Unit, National Hospital Sri Lanka, Colombo 07, Sri Lanka
Rasika Perera, Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
Article Tools
Follow on us
Abstract
Glutathione peroxidase and paraoxonase-1 is reported to be a useful marker for monitoring cardiovascular disease. Due to the paucity of information on the association of above markers and severity of coronary artery disease in South Asian patients, case control study was performed with 85 patients (58 males and 27 females) 40-60 years of age confirmed as having coronary artery disease on coronary angiography findings and 85 age and sex matched healthy volunteers as controls. Blood samples were analyzed for serum paraoxonase-1 and erythrocyte Glutathione peroxidase activity in both groups and the severity of coronary artery disease was assessed using coronary angiographic scoring system based on vessel, stenosis and extent score. Patients with coronary artery disease showed significantly low paraoxonase-1 and Glutathione peroxidase activity compared to control subjects. However, according to the best cutoff value determined by receiver operating characteristic analysis for serum paraoxonase-1 (38μg/mL) did not show a significantly high sensitivity, negative predictive value and negative likelihood ratio when compared to erythrocyte Glutathione peroxidase (84.5U/gHb) in predicting the severity of coronary artery disease assessed by three angiographic scores. Glutathione peroxidase appears to be an accurate marker in ruling out major coronary vessel disease and luminal narrowing by atheroma.
Keywords
Glutathione Peroxidase, Paraoxonase, Vessel Score, Stenosis Score, Extent Score
To cite this article
Dinushka Wickramasinghe, Hemantha Peiris, Lal Gotabhaya Chandrasena, Vajira Senaratne, Rasika Perera, Paraoxonase-1 and Glutathione Peroxidase Activity as a Screening Tool in Detecting Severity of Coronary Artery Disease: Case Control Feasibility Study, American Journal of Biomedical and Life Sciences. Vol. 3, No. 6, 2015, pp. 120-126. doi: 10.11648/j.ajbls.20150306.14
Copyright
Copyright © 2015 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Weinbrenner T, Cladellas M, Covas MI, Fito M, Tomas M, Senti M, et al. High oxidative stress in patients with stable coronary artery disease. Atherosclerosis. 2003; 168: 99-106.
[2]
Dhalla NS, Temsah RM, Netticadan T. Role of oxidative stress in cardiovascular diseases. J Hypertens. 2000; 18: 655–73.
[3]
Witztum JL. The oxidative hypothesis of atherosclerosis. Lancet. 1994; 344: 793-795.
[4]
Blankenberg S, Rupprecht HJ, Bicket C, Torzewski M, Hafher G, Tieret L, et al. Glutathione peroxidase 1 acting and cardiovascular events in patients with coronary artery disease. N Engl J Med. 2003; 349 (17): 1605-13.
[5]
Aviram M, Rosenblat M. Paraoxonases 1, 2, and 3, oxidative stress, and macrophage foam cell formation during atherosclerosis development. Free Radical Biology & Medicine. 2004; 37: 1304-16.
[6]
Madamanchi NR, Vendrov A, Runge MS. Oxidative stress and vascular disease. Arterioscler Thromb Vasc Biol. 2005; 25 (1): 29-38.
[7]
Zachara BA, Ukleja-adamowicz M, Nartowicz E, Lecka J. Increased plasma glutathione peroxidase activity in patients with acute myocardial infarction. Med Sci Monit. 2001; 7 (3): 415-20.
[8]
Gensini GG. A more meaningful scoring system for determining the severity of coronary heart disease. Am J Cardiol. 1983; 51 (3): 606.
[9]
Reardon MF, Nestel PJ, Craig IH, Harper RW. Lipoprotein predictors of the severity of coronary artery disease in men and women. Circulation. 1985; 71 (5): 881-8.
[10]
Hamsten A, Walldius G, Szamosi A, Dahlen G, de Faire U. Relationship of angiographically defined coronary artery disease to serum lipoproteins and apolipoproteins in young survivors of myocardial infarction. Circulation.1986; 73 (6): 1097-110.
[11]
Sullivan DR, Thomas MB, Marwicks MB, Freedman B. A new method of scoring coronary angiograms to reflect extent of coronary atherosclerosis and improves correlation with major risk factors. Am Heart J. 1990; 119: 1262-6.
[12]
World Medical Association. World Medical Association Declaration of Helsinki Ethical Principles for Medical Research Involving Human Subjects. JAMA. 2013; 310 (20): 2191-4.
[13]
Perera PR, Wickramasinghe D, Peiris H., Chandrasena LG. and Senaratne V. Association between LDL, Apolipoprotein-B Apolipoprotein A-I and Lipoprotein(a) and Severity of Coronary Artery Disease Based on Coronary Angiography. JBM. 2015; 3, 53-61.
[14]
Grundy SM, Pasternak R, Greenland P, Smith S Jr, Fuster V. Assessment of cardiovascular risk by use of multiple risk factor assessment equation. A statement for healthcare professionals from the American Heart Association and the American College of cardiology. Circulation. 1999; 100: 1481-92.
[15]
Chandrasena LG, Peiris H, Waikar HD. Biochemical changes associated with reperfusion after off-pump and on-pump coronary artery bypass graft surgery. Annl Clin lab Sci. 2009; 39 (4): 372-7.
[16]
Chandrasena LG, Peiris H, Kamani J, Wanigasuriya P, Jayaratne SD, Wijayasiri WA, et al. Antioxidants in patients with dengue viral infection. Southeast Asian J Trop Med Public Health. 2014; 45 (5): 1015-22.
[17]
Zhou C, Cao J, Shang L, Tong C, Hu H, Wang H, et al. Reduced Paraoxonase 1 activity as a marker for severity of coronary artery disease. Disease Markers. 2013; 35: 97-103.
[18]
Mateo GF, Santisteve PC, elosua R, Guallar E, Marrugat J, Bleys J, et al. Antioxidant enzyme activity and coronary heart disease: Meta-analyses of observational studies. Am J of Epidemiol. 2009; 170: 135-47.
[19]
Shaikh AK, Suryakar AN. Oxidative stress and antioxidant status before and after supplementation of A-Z anti-oxidant tablets in coronary artery disease. Biomedical Research 2009; 20 (2): 136-40.
[20]
Reddy ST, Devarajan A, Bourquard N, Shih D, Fogelman AM. Is it just Paraoxonase-1 or are other members of the paraoxonase gene family implicated in atherosclerosis? Curr Opin Lipidol. 2008; 19: 4005-8.
[21]
Jayakumari N, Thejaseebai G. High prevalence of low serum Paraoxonase-1 in subjects with coronary Artery disease. J Clin Biochem Nutr. 2009; 45 (3): 278-84.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186