Cellulase Enzyme Activity of Bacillus Circulans from Larvae Cossus Cossus in Lignocellulosic Substrat
American Journal of Biomedical and Life Sciences
Volume 4, Issue 2, April 2016, Pages: 21-25
Received: Feb. 19, 2016; Accepted: Mar. 6, 2016; Published: Mar. 28, 2016
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Authors
Maswati Baharuddin, Departement of Chemistry, Islamic State of Alauddin Universty, South Sulawesi, Indonesia
Ahyar Ahmad, Department of Chemistry, Graduate School of Science, Hasanuddin University, Makassar, South Sulawesi, Indonesia
Nursiah La Nafie, Department of Chemistry, Graduate School of Science, Hasanuddin University, Makassar, South Sulawesi, Indonesia
Firdaus Zenta, Department of Chemistry, Graduate School of Science, Hasanuddin University, Makassar, South Sulawesi, Indonesia
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Abstract
Cellulase are the enzymes hydrolyzing cellulosic biomass and are produced by the microorganism that grown over cellulosic matter. Bacterial Cellulases poses more advantage when compared to the cellulases from other sources. This study aims to determine the optimum conditions of cellulase production from the bacteria Bacillus Circulans CC2 and CC4 in comparison with isolates hydrolize rice straw. CC2 and CC4 isolated from larvae Cossus cossus. The testing to produce cellulase was done with various pH and temperature. The enzyme activity was tested using DNS Method. The results showed that the isolated CC2 and CC4 have the same optimum temperature of 70C on condition CMC 1%, medium pH 7,0. Crude extract cellulase isolates CC2 work optimally at pH 4 while the isolates CC4 work at pH 7. highest activity in the cellulase enzymes hydrolyze the substrate on cellulose powder with optimum activity at pH 4 CC4 isolates of 2.775 x 10-2 U/ mL, while the enzyme activity CC2 isolates 4.359 x 10-2 U/mL, p this occurs due to the enzymatic hydrolysis of cellulosic substrates with cellulase enzymes will outline the cellulose into glucose which is a simple form.
Keywords
Cellulace, Cossus Cossus, Rice Straw
To cite this article
Maswati Baharuddin, Ahyar Ahmad, Nursiah La Nafie, Firdaus Zenta, Cellulase Enzyme Activity of Bacillus Circulans from Larvae Cossus Cossus in Lignocellulosic Substrat, American Journal of Biomedical and Life Sciences. Vol. 4, No. 2, 2016, pp. 21-25. doi: 10.11648/j.ajbls.20160402.13
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Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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