Methicillin Resistant Staphylococcus aureus (MRSA) in Patients of Community Based Medical College Hospital, Mymensingh, Bangladesh
American Journal of Biomedical and Life Sciences
Volume 4, Issue 3, June 2016, Pages: 26-29
Received: Mar. 17, 2016; Accepted: Mar. 28, 2016; Published: Apr. 21, 2016
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Authors
Khalid Hussain, Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
Marzia Rahman, Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
K. H. M. Nazmul Hussain Nazir, Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
Hasibur Rahman, Department of Skin & VD, Community Based Medical College, Bangladesh, Mymensingh, Bangladesh
Abul Khair, Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
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Abstract
The rapid rise in resistance of Staphylococcus aureus to various antimicrobial agents is now a growing concern. The objectives of this study were to investigate the frequency of Methicillin Resistant S. aureus (MRSA) in different cases of infection, and to determine the pattern of antimicrobial susceptibility among the S. aureus originated from human in Mymensingh, Bangladesh. Covering a period of five months during July 2014 to November 2014, a total of 65 samples comprises of pus, wounds, eye lesions, burns were collected from the Community Based Medical College Hospital (CBMCH), Mymensingh, Bangladesh, and the samples were processed at the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh-2202. Among the 65 samples, 20% (n=13/65) were confirmed as S. aureus on the basis of colony morphology, staining characteristics, biochemical properties, and Polymerase Chain Reaction (PCR) by amplification of nuc gene (amplicon size 279-bp). Among these 13 S. aureus isolates, 15.38% (n=2/13) were found to be resistant to Methicillin which was confirmed by antibiotic disk sensitivity test and PCR by amplifying MRSA specific mecA gene (amplicon size 533-bp). The antibiotic disk sensitivity test indicated that the MRSA isolates were completely (100%) resistant to Penicillin and Erythromycin. The MRSA represents a major challenge for treatment in hospital due to the emergence and spread of antibiotic resistant isolates among susceptible individual.
Keywords
nuc Gene, mecA Gene, MRSA, Human, Vancomycin
To cite this article
Khalid Hussain, Marzia Rahman, K. H. M. Nazmul Hussain Nazir, Hasibur Rahman, Abul Khair, Methicillin Resistant Staphylococcus aureus (MRSA) in Patients of Community Based Medical College Hospital, Mymensingh, Bangladesh, American Journal of Biomedical and Life Sciences. Vol. 4, No. 3, 2016, pp. 26-29. doi: 10.11648/j.ajbls.20160403.11
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Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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