Carbohydrate Catabolism in Adult Onchocerca volvulus: An Immunohistochemical Study
American Journal of Biomedical and Life Sciences
Volume 4, Issue 3, June 2016, Pages: 35-40
Received: Apr. 14, 2016; Accepted: Apr. 25, 2016; Published: May 11, 2016
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Authors
Seidu Mahmood Abdulai, Department of Medical laboratory science, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana
Adams Abdul Rashid, Department of Medical laboratory science, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana
Gyasi Kwame Richard, Department of Pathology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana
Tettey Yao, Department of Pathology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana
Adunyame Lois, Department of Medical laboratory science, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana
Nkansah Obenewaa Dinah, Department of Medical laboratory science, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana
Wiredu Kwame Edwin, Department of Medical laboratory science, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana
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Abstract
Onchocerca volvulus is a parasite responsible for Onchocerciasis whose main pathology is blindness. Existing treatment and control approaches are not entirely successful, with some, fraught with safety challenges. Due to these problems, the need for developing safer and effective drugs to combat the disease has become imperative. However O. volvulus materials are restricted by ethical concerns due to its strict human preference. To overcome these concerns some researchers use animal models of closely related species to obtain biological information on O. volvulus and drugs developed from these sources of information have so far failed to kill the adult O. volvulus. Realistic targets for drug development against O. volvulus could be detected directly in O. volvulus rather than its closely related species. We performed immunohistochemical detection of three major enzymes (G6PD, LDH and PDHK2) involved in carbohydrate metabolism on paraffin processed archival O. volvulus nodules. We observed that up to 64.5% of worms in the paraffin processed nodules had detectable LDH, 61.1% had G6PD and 56.7% had PDHK2 and that most of the enzymes were stored in the muscles of the adult worm. These observations suggest that the adult O. volvulus can operate the glycolytic, Pentose and Entner-Douhoroff pathways either independently or concurrently suggesting that any drug aimed at preventing the adult worm from utilising carbohydrates must target all three enzymes.
Keywords
Antibodies, Antigens, Carbohydrate catabolism, Onchocerciasis, Nodules, Enzymes
To cite this article
Seidu Mahmood Abdulai, Adams Abdul Rashid, Gyasi Kwame Richard, Tettey Yao, Adunyame Lois, Nkansah Obenewaa Dinah, Wiredu Kwame Edwin, Carbohydrate Catabolism in Adult Onchocerca volvulus: An Immunohistochemical Study, American Journal of Biomedical and Life Sciences. Vol. 4, No. 3, 2016, pp. 35-40. doi: 10.11648/j.ajbls.20160403.13
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Hotez PJ, Kamath A. Neglected tropical diseases in Sub-Saharan Africa: review of their prevalence distribution, and disease burden. PLoS Negl Trop Dis 2009; 3:e412.
[2]
Taylor MJ, Hoerauf A, Bockarie M. Lymphatic filariasis and onchocerciasis. Lancet. 2010; 376:1175–85.
[3]
Bradley, J. E. Onchocerciasis. In F. E. G. Cox, J. P. Kreier & D. Wakelin (Eds.), Topley & Wilson’s Microbiology & Microbial Infections: Parasitology. Washington, D. C.: ASM Press. 2005; 10th ed., pp. 781-801.
[4]
Udall, D. N. (2007). Recent updates on onchocerciasis: Diagnosis and treatment. Clinical Infectious Diseases, 44(1), 53-60.
[5]
Enk, C. D. Onchocerciasis-river blindness. Clinics in Dermatology.2006; 24(3):176-180.
[6]
Traore MO, Sarr MD, Badji A, Bissan Y, Diawara L, et al. (2012) Proof-ofprinciple of onchocerciasis elimination with ivermectin treatment in endemic foci in Africa: Final results of a study in Mali and Senegal. PLOS Neg Trop Dis6: e1825.
[7]
Tarib B. Higazi, Timothy G. Geary, Charles D. Mackenzie. Chemotherapy in the treatment, control, and elimination of human onchocerciasis. Research and Reports in Tropical Medicine.2014:5 77-93.
[8]
Hiroyuki Takaoka, Takeshi Suzuki. Epidemiology and Control of Guatemalan Onchocerciasis. Tropical Medicine and Health Vol. 43 Supplement, 2015, 87–94
[9]
Lovato R, Guevara A, Guderian R, et al. Interruption of infection transmission in the onchocerciasis focus of Ecuador leading to the cessation of ivermectin distribution. PLoS Negl Trop Dis. 2014; 8:e2821.
[10]
Osei-Atweneboana MY, Awadzi K, Attah SK, Boakye DA, Gyapong JO, Prichard RK. Phenotypic evidence of emerging ivermectin resistance in Onchocerca volvulus. PLoS Negl Trop Dis. 2011; 5(3):e998.
[11]
Taylor MJ, Awadzi K, Basanez MG, Biritwum N, Boakye D, Boatin B, et al. Onchocerciasis control: vision for the future from a Ghanian perspective. Parasit Vectors. 2009; 2(1):7.
[12]
WHO, 2015. Investing to overcome the global impact of neglected tropical diseases: Third WHO report on neglected tropical diseases.
[13]
Basáñez, M. G., Pion, S. D., Churcher, T. S., Breitling, L. P., Little, M. P., Boussinesq, M., 2006. River blindness: a success story under threat? PLoS Med. Sep; 3(9):e371
[14]
Fukuda M, Takaoka H, Uni S, Bain O. Infective larvae of five Onchocerca species from experimentally infected Simulium species in an area of zoonotic onchocerciasis in Japan. Parasite. 2008 Jun; 15(2):111-9
[15]
Takaoka H1, Fukuda M, Otsuka Y, Aoki C, Uni S, Bain O. Blackfly vectors of zoonotic onchocerciasis in Japan. Med Vet Entomol. 2012 Dec; 26(4):372-8.
[16]
Sunisha. I. P., Rajendran R., Mani T. R., Munirathinam A. Reuben R., Dash. A.P. Impact of single dose of diethylcarbamazine and other antifilarial drug combinations on bancroftian filarial infection variables: Assessment after 2 years. Parasitology International. Volume 55, Issue 3, September 2006, Pages 233–236.
[17]
McCarthy, J. S. & Moore, T. A. 2015. 42 - Drugs for Helminths A2 - Bennett, John E. In: DOLIN, R. & BLASER, M. J. (eds.) Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases (Eighth Edition). Philadelphia: Content Repository Only!
[18]
Seidu, M. A., Adams, A. R., Gyasi, R. K., Tettey, Y. Nkansah, D. O., Wiredu. E. K., 2013. Immunoreactivity of some epitopes in longtime inappropriately stored paraffin embedded Tissues. Journal of Histotech. (ISSN: 01478885). Online: DOI: 10.1179/2046023613Y.0000000024
[19]
Seidu, M. A., Adams, A. R., Gyasi, R. K., Tettey, Y Wiredu, E. K., 2012. Short time incubation at low temperature for retrieval of some antigens from formalin-fixed and paraffin-embedded tissues. Journal of Histotech. vol. 35 no. 2 p74-79.
[20]
Jonathan Eze & Matur Bernard Malau. Assessment of the epidemiology of Onchocerca Volvulus after treatment with Ivermectin in the federal Capital territory, Abuja .Nigeria. IJRRAS 7 (3) June 2011.
[21]
Martin Walker, Sabine Specht, Thomas S. Churcher, Achim Hoerauf, Mark J. Taylor, María-Gloria Basáñez. Therapeutic Efficacy and Macrofilaricidal Activity of Doxycycline for the Treatment of River Blindness. Clin Infect Dis. 2015 Apr 15; 60(8):1199-207.
[22]
Van den Bossche H, editor. Comparative biochemistry of parasites. Elsevier; 2012 Dec 2.
[23]
Saxena J. K. Biochemistry of Microbes: Parasite biochemistry.2006 Dec 18.
[24]
Frank, J. E., 2005. Diagnosis and management of G6PD deficiency. Am. Fam. Physician 72 (7):1277–82.
[25]
A.J, Esan. "Effect of G6PD Status in Malaria Infected Individuals on Haematological Parameters." International Journal of Hematological Disorders 1.1 (2014):55-57
[26]
Sapna Gupta, Arvind K. Srivastava. Biochemical targets in filarial worms for selective antifilarial drug design. Acta Parasitologica, 2005, 50(1), 1–18; ISSN 1230-2821.
[27]
Strote, G., Bokhof, A., Comley, J. W. C., 1993. Viability of Onchocerca volvulus in-vitro. Parasit. 107, 175-182.
[28]
Muller, M., Mentel, M., Van Hellemond, J. J.,Henze, K., Woehle, C., Gould, S. B., Yu, R.-Y., Van Der Giezen, M., Tielens, A. G. M. & Martin, W. F. 2012. Biochemistry and Evolution of Anaerobic Energy Metabolism in Eukaryotes. Microbiology and Molecular Biology Reviews: MMBR, 76, 444-495.
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