Effect of Pectin on the Activity of Pancreatic α-Amylase in Growing Rats with Lead Intoxication
American Journal of Biomedical and Life Sciences
Volume 4, Issue 5, October 2016, Pages: 69-74
Received: Aug. 13, 2016; Accepted: Aug. 23, 2016; Published: Sep. 9, 2016
Views 3363      Downloads 210
Authors
Lubov Salijanovna Kuchkarova, Department of Physiology and Neurobiology, National University of Uzbekistan, Tashkent, Uzbekistan
Gulchehkra Tengelbaevna Kudeshova, Department of Physiology and Neurobiology, National University of Uzbekistan, Tashkent, Uzbekistan
Article Tools
Follow on us
Abstract
This study was conducted to know the effect of pectin on the α-amylase activity in the pancreas, intestine content and blood in growing rats with lead intoxication. After a single oral administration of lead acetate (80 mg/kg) to growing rats pectin was administered (400 mg/kg/12h) for 4 days. It is revealed that the administration of lead acetate causes an increase in pancreas weight, protein content in the pancreas and α-amylase activity in the pancreas and blood and reduced activity of the enzyme in the intestinal cavity in rats. Regular oral administration of pectin has a normalizing effect on pancreatic weight, protein content and on the level of the activity α-amylase in the pancreas, intestinal contents and blood in the 48-72 hours of observation in rats. These results support our suggestion about the possibility of the use of pectin for correcting of pancreatitis similar shifts at lead intoxication.
Keywords
Rat, Lead Acetate, Pectin, α-Amylase, Pancreas
To cite this article
Lubov Salijanovna Kuchkarova, Gulchehkra Tengelbaevna Kudeshova, Effect of Pectin on the Activity of Pancreatic α-Amylase in Growing Rats with Lead Intoxication, American Journal of Biomedical and Life Sciences. Vol. 4, No. 5, 2016, pp. 69-74. doi: 10.11648/j.ajbls.20160405.11
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Komissarenko SN, Spiridonov VN. Pektiny – ih svojstva i primenenie. Rastitel'nye resursy. 1998; 34 (1): 111-119.
[2]
Ovodov JuS. Sovremennye predstavlenija o pektinovyh veshhestvah. Bioorganicheskaja. himija. 2009; 5 (3): 293-310.
[3]
Hotimchenko JuS, Ermak IM, Bednjak AE, Hasina JeI, Kropotov AV, Kolenchenko EA, Sergushhenko IS, Hotimchenko MJu, Kovalev VV. Farmakologija nekrahmal'nyh polisaharidov. Vestnik DVO RAN (Vestnik dal'nevostochnogo otdelenija Rossijskoj akademii nauk). 2005; (1): 72-82.
[4]
Thakur BR, Singh RK, Handa AK. Chemistry and uses of pectin-a review. Crit Rev Food Sci Nutr. 1997 Feb; 37 (1): 47-73.
[5]
El-Zoghbi M, Sitohy MZ. Mineral absorption by albino rats as affected by some types of dietary pectins with different degrees of esterification. Molecular nutrition food research. 2001; 45: 114-117.
[6]
Flora G, Gupta D, Tiwari A. Toxicity of lead: A review with recent updates. Interdiscip Toxicol. 2012; 5 (2): 47–58.
[7]
Kalia K, Flora SJ. Strategies for safe and effective therapeutic measures for chronic arsenic and lead poisoning. J Occup Health. 2005; 47: 1–21.
[8]
Patrick L. Lead toxicity, a review of the literature. Part 1: Exposure, evaluation, and treatment. Alternative medicine review. 2006; 11 (1): 2–22.
[9]
Kolosova II. Vlijanie acetata svinca, solej tjazhelyh metallov na reproduktivnuju funkciju. Vestnik problem biologii i mediciny (Ukraina).- 2013; 2 (103): 13-14.
[10]
Verity AM. Comparative observations on inorganic and organic lead neurotoxicity. Environmental health perspectives. 1990; 89: 43-48.
[11]
Izmerov NF. Svinec i zdorov'e. Gigienicheskij i mediko-biologicheskij monitoring. Moskva 2000, 256s.
[12]
Landrigan PJ. The Worldwide Problem of Lead in Petrol. Bulletin of the WHO. 2002; 80: 10.
[13]
Cretaccia Y, Parsons JP. Localized accumulation of lead within and among bones from lead-dosed goats. Environ Res. 2010; 110 (1): 26–32.
[14]
Burak ZhM,. Sukalo AV, Terehova TN. Vozdejstvie svincovoj intoksikacii na cheloveka i zhivotnyh, vlijanie na razvitie i funkcii zubocheljustnoj sistemy. Medicinskij zhurnal (Ukraina). 2005; (4): 10-13.
[15]
Dees WL. Effect of lead exposure during gestation and lactation on femal pubertal development in the rat. Reprod Toxicol. 2002; 16 (4): 343-52.
[16]
Inomov AU., Zhabborov AM, Musaev DM, Kudeshova GT, Kuchkarova LS. Vlijanie svincovoj intoksikacii u materina nachal'nye i zakljuchitel'nye stadii gidroliza uglevodov u potomstva. Infekcija, immunitet, farmakologija. 2014; (1): 47-50.
[17]
Ahmed MB, Ahmed MI, Meki A-R and AbdRaboh N. Neurotoxic effect of lead on rats: Relationship to Apoptosis. Int J Health Sci (Qassim). 2013; 7 (2): 192–199.
[18]
Gryzlova LV, Ponjaev VV, Kozhaev RV. Vlijanie dlitel'noj intoksikacii svincom beremennyh samok belyh krys na osteoplasticheskie processy v organizme ih potomstva. Nauchnoe periodicheskoe izdanie «in situ ». 2015; (3): 14-15.
[19]
Gryzlova LV, Tel'cov LP., Shubina OS, Fedotova GG. Aktivnost' hondrocitov krysjat, materi kotoryh poluchali acetat svinca. Morfologija. 2009; (4): 43.
[20]
Missoun F, Slimani, M, Aoues A. Toxic effect of lead on kidney function in rat Wistar. African Journal of Biochemistry Research. 2010; 4 (2): 21-27.
[21]
Reza B, Ali N, Azhdar H, Alireza A, Ali K. Effects of low-level lead exposure on blood pressure and function of the rat isolated heart. Indian J Pharmacol. 2008 Mar-Apr; 40 (2): 69–72.
[22]
Der R, Yousef M, Fahim Z, Fahim M. Effects of lead and cadmium on adrenal and thyroid functions in rats. Res Commun Chem Pathol Pharmacol. 1977 Jun; 17 (2): 237-53.
[23]
Zadjali SA, Nemmar A, Mohamed Abdelmonem AY Fahim MAA, Azimullah S, Subramanian D, Yasin J, Amir N, Hasan MY, Adem A. Lead exposure causes thyroid abnormalities in diabetic rats. Int J Clin Exp Med. 2015; 8 (5): 7160–7167.
[24]
Gorbel F, Boujelbene M, Makni –Ayadi F, Guermazi F, Croute F, Soleilhavoup JP, EL-Feki A. Sytotoxic effect of lead on the endocrine and exocrine sexual function of pubescent male and female rats. Demonstration of apoptotic activity. CR BIO. 2002; 325 (9): 927-40.
[25]
Lugovskoj SP, Legkostup LA. Mehanizmy biologicheskogo dejstvija svinca na pishhevaritel'nuju sistemu. Suchasnі problemi toksikologії. 2002; (2): 45-50.
[26]
Siposet P, Szentmihályi K, Fehér E, Abaza M, Szilyági M, Blázovics A. Some effects of lead contamination on liver and gall bladder bile. Acta Biol Szeged. 2003; 47 (1-4): 139-42.
[27]
Metwally ESAM, Negin FA, El-din RAS, Nabil EM. Anatomical and histological study of the effect of lead on hepatocytes of albino rats. IJBMR. 2015; 3 (4): 34-45.
[28]
Liska DJ. The detoxification enzyme systems. Altern Med Rev. 1998 (Jun); 3 (3): 187–198.
[29]
Abdollahi M, Sharifzadeh M, Marzban H, Torab-Jahromi M. Alteration by lead acetate of rat submandibular gland morphology and ultrastructure. Toxic Substance Mechanisms. 1999; 18 (3): 139-147.
[30]
Korot'ko GF. Reguljacija jekzosekrecii podzheludochnoj zhelezy // Uspehi fiziologicheskih nauk. 2009; 40 (1): 27-43.
[31]
Meisterfeld R, Ehehalt F, Saeger HD, Solimena M. Pancreatic disorders and diabetes mellitus. Exp Clin Endocrinol Diabetes. 2008 Sep; 116 Suppl 1: 7-12.
[32]
Jergashev NA, Kuchkarova LS. Polovaja specifichnost' izmenenija aktivnosti pankreaticheskoj a-amilazy u krys pri kadmievoj intoksikacii. Rossijskij fiziologicheskij zhurnal imeni I. M. Sechenova. 2011; 97 (5): 525-535.
[33]
Kuchkarova LS, Sadykov BA, Jergashev NA, Dustmatova GA. Faktory regulirujushhie aktivnost' α-amilazy u rastushhego organizma. Pediatrija. 2007; (1-2): 114-117.
[34]
Sychev IA, Smirnov VM, Kolosova TJu. Dejstvie polisaharidov zheltogo donnika na sistemu krovi obluchennyh zhivotnyh. Rossijskij mediko-biologicheskij vestnik imeni akademika I. P. Pavlova. 2006: (1): 51-55.
[35]
Ugolev A. M. Opredelenie amiloliticheskoj aktivnosti // Issledovanie pishhevaritel'nogo apparata u cheloveka - L.: Nauka, 1969: 187–192.
[36]
Lowry OH, Rosebrough NJ, Farr AL, and Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951; 193 (1): 265–75.
[37]
Ibrahim NM, Eweis EA, El-Beltagi HS, Abdel-Mobdy YE. Effect of lead acetate toxicity on experimental male albino rat. Asian Pac J Trop Biomed. 2012; 2: 41-46.
[38]
Sadykov BA, Kuchkarova LS, Jermatova SA, Jergashev N A. Aktivnost'. pishhevaritel'nyh fermentov u potomstva krys, poluchavshih vo vremja laktacii racion, kontaminirovannyj tjazhjolymi metallami. Voprosy pitanija (Rossija). 2009; (2): 47–50.
[39]
Haliullina SV. Klinicheskoe znachenie deficita cinka v organizme rebenka. Vestnik sovremennoj klinichechkoj mediciny. 2013; 6 (3): 72-77.
[40]
Sakata S, Shimizu S, Ogoshi K, Hirai K, Ohno Y, Kishi T, Sherchand JB, Utsumi M, Shibata M, Takaki M, Ueda M, Mori I. Inverse relationship between serum erythropoietin and blood lead concentrations in Kathmandu tricycle taxi drivers. Int Arch Occup Environ Health. 2007; 80 (4): 342-45.
[41]
Kostjukevich O. I. Pishhevaritel'naja nedostatochnost' i ee korrekcija s pozicii vracha-gastrojenterologa. Rossijskij medicinskij zhurnal. 2008; 10 (1): 10-14.
[42]
Agapov MA, Gorskij VA, Petrov VA, Polivoda MD, Kravchenko AJu, Battaeva AI. Sposoby modelirovanija ostrogo pankreatita (Obzor literatury). Vestnik RGMU (Rossijskij gosudartvennyj medicinskij universitet). 2014; (3): 25-39.
[43]
Andrzejewska A, Szynaka B, Stokowska W, Szynaka P. Does lead acetate intoxication damage acinar cell nuclei in the rat pancreas. Mater Med Pol. 1995 Apr-Jun; 27 (2): 43-46.
[44]
Isenman L., Liebow C., Rothman S. The endocrine secretion of mammalian digestive enzymes byexocrine glands. Am J Physiol. 1999 Feb; 276 (2 Pt 1): E223-232.
[45]
Permjakov NK., Podol'skij AE., Titova GP. Ul'trastrukturnyj analiz sekretornogo cikla podzheludochnoj zhelezy. Moskva: Medicina, 1973. – 238 s.
[46]
Korot'ko G. F. Rekrecija fermentov i gormonov jekzokrinnymi zhelezami. Uspehi fiziol nauk. 2003; 34 (2): 21–32.
[47]
Chegodaeva AA, Funygin MS. Strukturno-funkcional'naja harakteristika izmenenij v podzheludochnoj zheleze v uslovijah jeksperimental'nogo ostrogo pankreatita u morskih svinok Bjulljuten' VSNC SO RAMN (Bjulljuten' vostochnosibirskogo nauchnogo centra Sibirskogo otdelenija Rossijskoj akademii medicinskih nauk). 2012; 86 (4): 210-212.
[48]
Khotimchenko M, Kovalev V, and Khotimchenko Y. Equilibrium studies of sorption of lead (II) ions by different pectin compounds. J. Hazard. Mater. 2007; 149: 693-699.
[49]
Khasina. EI., Kolenchenko EA., Sgrebneva MN, Kovalev VV., Khotimchenko YuS. Antioxidant Activities of a Low Etherified Pectin from the Seagrass Zostera marina. Russian Journal of Marine Biology 2003 Jul; 29: 259-261.
[50]
El-Nahal DM Effect of using pectin on lead toxicity. J Am Sci. 2010; 6 (12): 541-554.
[51]
Trahtenberg IM, Korolenko TK, Muradjan HK, Utko NA. vlijanie svinca na razvitie okislitel'nogo stressa. Toksikologicheskij vestnik. 2002; (3); 22-26.
[52]
Schoenberg MH, Birk D, Beger HG. Oxidative stress in acute and chronic pancreatitis. Am J Clin Nutr. 1995; 62: 1306S–1314S.
[53]
Mosojan SS, Shugaev AI, Chujanova EV Perekisnoe okislenie lipidov i antioksidantnaja zashhita pri ostrom destrusktivnom pankreatite. Vestnik Sankt-Peterburgskogo universiteta. 2014; 11 (2): 97-103.
[54]
Chun W, Bamba T, Hosoda S. Effect of pectin, a soluble dietary fiber, on functional and morphological parameters of the small intestine in rats. Digestion. 1989; 42: 22–29.
[55]
Pirman T, Ribeyre MC, Mosoni L, Rémond D, Vrecl M, Salobir J, Mirand PP. Dietary pectin stimulates protein metabolism in the digestive tract. Nutrition. 2007 Jan; 23 (1): 69-75.
ADDRESS
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
U.S.A.
Tel: (001)347-983-5186