Evaluation of Pro-Inflammatory Cytokines Expression in Mouse Splenocytes After Incubation with Biofield Treated Herbomineral Formulation: Effect of Biofield Energy Healing Treatment - The Trivedi Effect®
American Journal of Biomedical and Life Sciences
Volume 4, Issue 6, December 2016, Pages: 87-97
Received: Nov. 3, 2016; Accepted: Nov. 17, 2016; Published: Dec. 8, 2016
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Authors
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton, Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, Nevada, USA
Alan Joseph Balmer, Trivedi Global, Inc., Henderson, Nevada, USA
Dimitrius Anagnos, Trivedi Global, Inc., Henderson, Nevada, USA
Janice Patricia Kinney, Trivedi Global, Inc., Henderson, Nevada, USA
Joni Marie Holling, Trivedi Global, Inc., Henderson, Nevada, USA
Joy Angevin Balmer, Trivedi Global, Inc., Henderson, Nevada, USA
Lauree Ann Duprey-Reed, Trivedi Global, Inc., Henderson, Nevada, USA
Vaibhav Rajan Parulkar, Trivedi Global, Inc., Henderson, Nevada, USA
Mayank Gangwar, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
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Abstract
The use of herbomineral formulations in the healthcare sector has increased due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with a mixture of the herbal root extract of ashwagandha and three minerals viz. zinc chloride, magnesium gluconate, and sodium selenate. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the formulation when applied to splenocyte cells isolated from mice spleen. The formulation was divided into two parts; one was the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers. The test formulation was evaluated to find the expression of pro-inflammatory cytokines such as TNF-α, MIP-1α, and IL-1β along with non-cytotoxic concentrations by MTT assay. The splenocytes were given the Biofield Energy Treated and untreated sample at concentrations range (0.00001053 to 10.53 µg/mL) for 48 hours and was reported with safe concentration up to 1.053 µg/mL with percentage viability range from 76.7% to 109.2% in both samples. Biofield Energy Healing significantly enhanced the cell viability as compared with the untreated formulation. The expression of TNF-α was significantly inhibited in the Biofield Treated formulation at 0.01053, 0.1053, and 1.053 µg/mL by 1.77%, 1.93%, and 3.55%, respectively compared with the untreated formulation. The rest of the tested concentrations of the Biofield Treated formulation showed an increase in TNF-α expression at 0.00001053, 0.0001053, and 0.001053 µg/mL by 7.26%, 8.50%, and 8.50%, respectively compared to the vehicle control group. Similarly, the MIP-1α expression was inhibited by the Biofield Energy Treated formulation and showed immunosuppression activity at 0.01053 µg/mL by 18.47% (p≤0.001) compared to the untreated formulation. MIP-1α expression was reported as 628.94 ± 13.0 pg/mL in the untreated formulation, while it decreased to 512.74 ± 1.9 pg/mL in the Biofield Treated formulation at 0.01053 µg/mL. In addition, the IL-1β secretion was also significantly inhibited by the Biofield Treated formulation at concentrations 0.001053, 0.01053, 0.1053, and 1.053 µg/mL by 72.02%, 50.16%, 30.68%, and 22.11%, respectively as compared with the untreated formulation. Overall, The Trivedi Effect® significantly down-regulated the pro-inflammatory cytokines and potentiated the immunosuppressive effect of the treated formulation, which can be better utilized in organ transplants, anti-aging, stress management, autoimmune disorders, and inflammatory disorders, etc. to modulate the immune system by improving overall health.
Keywords
Biofield Energy Healing Treatment, Biofield Energy Healers, The Trivedi Effect®, LPS, Pro-Inflammatory Cytokines, TNF-α, MIP-1α, IL-1β
To cite this article
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Alan Joseph Balmer, Dimitrius Anagnos, Janice Patricia Kinney, Joni Marie Holling, Joy Angevin Balmer, Lauree Ann Duprey-Reed, Vaibhav Rajan Parulkar, Mayank Gangwar, Snehasis Jana, Evaluation of Pro-Inflammatory Cytokines Expression in Mouse Splenocytes After Incubation with Biofield Treated Herbomineral Formulation: Effect of Biofield Energy Healing Treatment - The Trivedi Effect®, American Journal of Biomedical and Life Sciences. Vol. 4, No. 6, 2016, pp. 87-97. doi: 10.11648/j.ajbls.20160406.12
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Thomson GE (2007) The Health Benefits of Traditional Chinese Plant Medicines: Weighing the Scientific Evidence: A Report for the Rural Industries Research and Development Corporation, RIRDC, Barton, Australia.
[2]
Rishton GM (2008) Natural products as a robust source of new drugs and drug leads: Past successes and present day issues. Am J Cardiol 101: 43D-49D.
[3]
Darien BJ, Godbee RJ (2009) Morinda citrifolia based formulations for regulating T cell immunomodulation in neonatal stock animals. USA Patent 20090068204.
[4]
Ziauddin M, Phansalkar N, Patki P, Diwanay S, Patwardhan B (1996) Studies on the immunomodulatory effects of ashwagandha. J Ethnopharmacol 50: 69-76.
[5]
Lukác N, Massányi P (2007) Effects of trace elements on the immune system. Epidemiol Mikrobiol Imunol 56: 3-9.
[6]
Galland L (1998) Magnesium and immune function: An overview. Magnesium 7: 290‐299.
[7]
Wintergerst ES, Maggini S, Hornig DH (2007) Contribution of selected vitamins and trace elements to immune function. Ann Nutr Metab 51: 301‐323.
[8]
Girdhari L, Rana A (2007) Withania somnifera (Ashwagandha): A review. Pharmacogn Rev 1: 129-136.
[9]
Owais M, Sharad KS, Shehbaz A, Saleemuddin M (2005) Antibacterial efficacy of Withania somnifera (Ashwagandha) an indigenous medicinal plant against experimental murine salmonellosis. Phytomedicine 12: 229-235.
[10]
Singh N, Bhalla M, de Jager P, Gilca M (2011) An overview on ashwagandha: A Rasayana (Rejuvenator) of Ayurveda. Afr J Tradit Complement Altern Med 8: 208-213.
[11]
Mazumder PM, Pattnayak S, Parvani H, Sasmal D, Rathinavelusamy P (2012) Evaluation of immunomodulatory activity of Glycyrhiza glabra L roots in combination with zing. Asian Pac J Trop Biomed 2: S15-S20.
[12]
Lutgendorf SK, Mullen-Houser E, Russell D, Degeest K, Jacobson G, Hart L, Bender D, Anderson B, Buekers TE, Goodheart MJ, Antoni MH, Sood AK, Lubaroff DM (2010) Preservation of immune function in cervical cancer patients during chemoradiation using a novel integrative approach. Brain Behav Immun 24: 1231-1240.
[13]
Ironson G, Field T, Scafidi F (1996) Massage therapy is associated with enhancement of the immune system's cytotoxic capacity. Int J Neurosci 84: 205-217.
[14]
Jain S, Hammerschlag R, Mills P, Cohen L, Krieger R, Vieten C, Lutgendorf S (2015) Clinical studies of biofield therapies: Summary, methodological challenges, and recommendations. Glob Adv Health Med 4: 58-66.
[15]
Rubik B (2002) The biofield hypothesis: Its biophysical basis and role in medicine. J Altern Complement Med 8: 703-717.
[16]
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol 4: 141.
[17]
Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In vitro evaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. J Cancer Sci Ther 7: 253-257.
[18]
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) In vitro evaluation of biofield treatment on Enterobacter cloacae: Impact on antimicrobial susceptibility and biotype. J Bacteriol Parasitol 6: 241.
[19]
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) Evaluation of biofield modality on viral load of hepatitis B and C Viruses. J Antivir Antiretrovir 7: 083-088.
[20]
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) An impact of biofield treatment: Antimycobacterial susceptibility potential using BACTEC 460/MGIT-TB System. Mycobact Dis 5: 189.
[21]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Antimicrobial sensitivity, biochemical characteristics and biotyping of Staphylococcus saprophyticus: An impact of biofield energy treatment. J Women’s Health Care 4: 271.
[22]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of antibiogram, genotype and phylogenetic analysis of biofield treated Nocardia otitidis. Biol Syst Open Access 4: 143.
[23]
Trivedi MK, Branton A, Trivedi D, Nayak G, Charan S, Jana S (2015) Phenotyping and 16S rDNA analysis after biofield treatment on Citrobacter braakii: A urinary pathogen. J Clin Med Genom 3: 129.
[24]
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Spectroscopic characterization of chloramphenicol and tetracycline: An impact of biofield. Pharm Anal Acta 6:395.
[25]
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Spectroscopic characterization of biofield treated metronidazole and tinidazole. Med Chem 5: 340-344.
[26]
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Effect of biofield treatment on spectral properties of paracetamol and piroxicam. Chem Sci J 6: 98.
[27]
Trivedi MK, Branton A, Trivedi D, Shettigar H, Bairwa K, Jana S (2015) Fourier transform infrared and ultraviolet-visible spectroscopic characterization of biofield treated salicylic acid and sparfloxacin. Nat Prod Chem Res 3:186.
[28]
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2016) Molecular analysis of biofield treated eggplant and watermelon crops. Adv Crop Sci Tech 4: 208.
[29]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Morphological characterization, quality, yield and DNA fingerprinting of biofield energy treated alphonso mango (Mangifera indica L.). Journal of Food and Nutrition Sciences 3: 245-250.
[30]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of plant growth, yield and yield attributes of biofield energy treated mustard (Brassica juncea) and chick pea (Cicer arietinum) seeds. Agriculture, Forestry and Fisheries 4: 291-295.
[31]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of plant growth regulator, immunity and DNA fingerprinting of biofield energy treated mustard seeds (Brassica juncea). Agriculture, Forestry and Fisheries 4: 269-274.
[32]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Jana S (2015) Characterization of physical and structural properties of aluminum carbide powder: Impact of biofield treatment. J Aeronaut Aerospace Eng 4: 142.
[33]
Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) Impact of biofield treatment on atomic and structural characteristics of barium titanate powder. Ind Eng Manage 4: 166.
[34]
Trivedi MK, Patil S, Nayak G, Jana S, Latiyal O (2015) Influence of biofield treatment on physical, structural and spectral properties of boron nitride. J Material Sci Eng 4: 181.
[35]
Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) Characterization of physical and structural properties of brass powder after biofield treatment. J Powder Metall Min 4: 134.
[36]
Wu QL, Fu YF, Zhou WL, Wang JX, Feng YH, Liu J, Xu JY, He PL, Zhou R, Tang W, Wang GF, Zhou Y, Yang YF, Ding J, Li XY, Chen XR, Yuan C, Lawson BR, Zuo JP (2005) Inhibition of S-adenosyl-l-homocysteine hydrolase induces immunosuppression. J Pharmacol Exp Ther 313: 705-711.
[37]
Madaan A, Kanjilal S, Gupta A, Sastry JL, Verma R, Singh AT, Jaggi M (2015) Evaluation of immunostimulatory activity of Chyawanprash using in vitro assays. Indian J Exp Biol 53: 158-163.
[38]
Bodeker G, Kronenberg F (2002) A public health agenda for traditional, complementary, and alternative medicine. Am J Public Health 92: 1582-1591.
[39]
Banerjee M (2009) Hyderabad: Orient Black Swan. Power Knowledge Medicine - Ayurvedic Pharmaceuticals at Home and in the World; pp. 154.
[40]
Joshi D (1998) Varanasi, India: Chaukambha Sanskrit Bhawan; 1998. Rasa Amritam; pp. 127-129.
[41]
Gao B, Radaeva S, Park O (2009) Liver natural killer and natural killer T cells: Immunobiology and emerging roles in liver diseases. J Leukoc Biol 86: 513-528.
[42]
Singh D, Aggarwal A, Maurya R, Naik S (2007) Withania somnifera inhibits NF-κB and AP-1 transcription factors in human peripheral blood and synovial fluid mononuclear cells. Phytother Res 21: 905-913.
[43]
Haase H, Rink L (2009) The immune system and the impact of zinc during aging. Immun Ageing 6: 9.
[44]
Sugimoto J, Romani AM, Valentin-Torres AM, Luciano AA, Ramirez Kitchen CM, Funderburg N, Mesiano S, Bernstein HB (2012) Magnesium decreases inflammatory cytokine production: A novel innate immunomodulatory mechanism. J Immunol 188: 6338-6346.
[45]
Chen YC, Sosnoski DM, Gandhi UH, Novinger LJ, Prabhu KS, Mastro AM (2009) Selenium modifies the osteoblast inflammatory stress response to bone metastatic breast cancer. Carcinogenesis 30: 1941-1948.
[46]
Laclaustra M, Navas-Acien A, Stranges S, Ordovas JM, Guallar E (2009) Serum selenium concentrations and hypertension in the US Population. Circ Cardiovasc Qual Outcomes 2: 369-376.
[47]
Navas-Acien A, Bleys J, Guallar E (2008) Selenium intake and cardiovascular risk: What is new? Curr Opin Lipidol 19: 43-49.
[48]
Seo Y, Lee HJ, Kim Y, Youn H, Lee BJ (2005) Effects of several salt marsh plants on mouse spleen and thymus cell proliferation using MTT assay. Ocean Science Journal 40: 209-212.
[49]
Durrieu C, Degraeve P, Carnet-Pantiez A, Martial A (2005) Assessment of the immunomodulatory activity of cheese extracts by a complete and easy to handle in vitro screening methodology. Biotechnol Lett 27: 969-975
[50]
Bemelmans MH, van Tits LJ, Buurman WA (1996) Tumor necrosis factor: Function, release and clearance. Crit Rev Immunol 16: 1-11.
[51]
Hsieh CH, Frink M, Hsieh YC, Kan WH, Hsu JT, Schwacha MG, Choudhry MA, Chaudry IH (2008) The role of MIP-1 alpha in the development of systemic inflammatory response and organ injury following trauma hemorrhage. J Immunol 181: 2806-2812.
[52]
Dinarello CA (2009) Immunological and inflammatory functions of the interleukin-1 family. Annu Rev Immunol 27: 519-550.
[53]
Schultz MJ, Rijneveld AW, Florquin S, Edwards CK, Dinarello CA, van der Poll T (2002) Role of interleukin-1 in the pulmonary immune response during Pseudomonas aeruginosa pneumonia. Am J Physiol Lung Cell Mol Physiol 282: L285-L290.
[54]
Farrar JD, Asnagli H, Murphy KM (2002) T helper subset development: Roles of instruction, selection, and transcription. J Clin Invest 109: 431-435.
[55]
Gronowicz G, Secor ER, Flynn JR, Jellison ER, Kuhn LT (2015) Therapeutic touch has significant effects on mouse breast cancer metastasis and immune responses but not primary tumor size. Evid Based Complement Alternat Med 2015: 926565.
[56]
Clarke TC, Black LI, Stussman BJ, Barnes PM, Nahin RL (2015) Trends in the use of complementary health approaches among adults: Unites States, 2002-2012. National health statistics reports; no 79. Hyattsville, MD: National Center for Health Statistics.
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