American Journal of Biomedical and Life Sciences
Volume 6, Issue 3, June 2018, Pages: 43-50
Received: May 27, 2018;
Accepted: Jun. 22, 2018;
Published: Jul. 16, 2018
Views 379 Downloads 25
Md. Masud Rana, Department of Pharmacy, Faculty of Science, University of Rajshahi, Rajshahi, Bangladesh
Alpana Khatun, Department of Pharmacy, Faculty of Science, University of Rajshahi, Rajshahi, Bangladesh
Md. Rafiqul Islam Khan, Department of Pharmacy, Faculty of Science, University of Rajshahi, Rajshahi, Bangladesh
Abu Syed Md Anisuzzaman, Department of Pharmacy, Faculty of Science, University of Rajshahi, Rajshahi, Bangladesh; Department of Radiation Oncology, Winship Cancer Institute, Emory University, Atlanta, USA
Mir Imam Ibne Wahed, Department of Pharmacy, Faculty of Science, University of Rajshahi, Rajshahi, Bangladesh
The biochemical parameters such as SGOT, SGPT, MDA, and uric acid levels were investigated for the assessment of stress-induced dysfunction after immobilization stress on the Swiss albino mice. Stress induction caused the elevation of SGOT (7.7 ± 0.2 vs 88.8 ± 0.3; control vs stress), SGPT (10.3 ± 0.5 vs 110.5 ± 5.2), MDA (3.9 ± 0.2 vs 9.4 ± 0.2 in the serum and 4.0 ± 0.3 vs 13.9 ± 0.2 in brain; control vs stress) and uric acid (7.0 ± 0.1 vs 17.2 ± 0.3; control vs stress) levels in the serum. Histopathology of the liver, kidney heart and lung were also examined, and morphology indicated the degradation of each of the organ by the stress induction. The bark of Loranthus globosus was extracted with methanol and then fractionated with petroleum ether (PE), chloroform (CF) and ethyl acetate (EA). Phytochemical screening confirmed the presence of flavanoids and phenolics in all fractions. The antioxidant activity was evaluated by in vitro assays using total antioxidant capacity and DPPH- free radical scavenging activity. The results demonstrated that among all the extractives of L. globosus EA fraction exhibited highest total antioxidant and free radical scavenging activity. The EA fraction of L. globosus (12 mg/kg b. w.) successfully reduced the increased biochemical parameters (stress vs stress + EAF); SGOT (88.8 ± 0.3 vs 9.9 ± 0.9), SGPT (110.5 ± 5.2 vs 14.6 ± 0.7), MDA (9.5 ± 0.2 vs 5.5 ± 0.1 in the serum; and 13.9 ± 0.2 vs 7.5 ± 0.2 in the brain) and serum uric acid (17.2 ± 0.3 vs 8.9 ± 0.1) on stress-induced mice. Histopathological analysis also supported the beneficial effects of EA fraction of L. globosus. Dexamethasone (2 mg/kg b. w.) was used as standard drug. The results suggested that the EA fraction of L. globosus might have some beneficial effects in preventing stress-induced organ dysfunction presumably through the neutralization of oxidative-stress generated during immobilization of mice. However, further study is necessary in order to precisely determine the exact molecular mechanisms.
Md. Masud Rana,
Md. Rafiqul Islam Khan,
Abu Syed Md Anisuzzaman,
Mir Imam Ibne Wahed,
Protective Effect of Loranthus globosus on Stress-Induced Major Organ Dysfunctions in Mice, American Journal of Biomedical and Life Sciences.
Vol. 6, No. 3,
2018, pp. 43-50.
M. Valko, H. Morris, M. T. Cronin. Metals, toxicity and oxidative stress. Curr. Med. Chem. 12(10), (2005) 1161-208.
M. D. Evans and M. S. Cooke. Factors contributing to the outcome of oxidative damage to nucleic acids. BioEssays, 26 (2004) 533–542.
V. J. Thannickal, B. L. Fanburg, Reactive oxygen species in cell signaling., Am J PhysiolLung Cell Mol Physiol 279 (2000) 1005-1008.
B. S. Berlett, E. R. Stadtman, Protein oxidation in aging, disease, and oxidative stress., J Biol Chem 272 (1997) 20313-20316.
H. Wiseman, B. Halliwell, Damage to DNA by reactive oxygen and nitrogen species: role in inflammatory disease and progression to cancer., Biochem J 313 (1996) 17-29.
K. S. Kumar, K. Ganesan, P. V. S. Rao, Antioxidant potential of solvent extracts of Kappaphycus alvarezii (Doty) Doty – An edible seaweed, Food Chemistry 107 (2007) 289-295.
J. Zhishen, T. Mengcheng, W. Jianming, The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals, Food Chemistry 64 (1999) 555-559.
Y. Pan, K. Wang, S. Huang, H. Wang, X. Mu, C. He, X. Ji, J. Zhang, F. Huang, Antioxidant activity of microwave-assisted extract of longan (Dimocarpus Longan Lour.) peel, Food Chemistry 106 (2008) 1264-1270.
P. Prieto, M. Pineda, M. Aguilar, Spectrophotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum Complex: Specific application to the determination of vitamin E, Analytical Biochemistry 269 (1999) 337-341.
A. S. M Anisuzzaman, S. Morishima, F. Suzuki, T. Tanaka, H. Yoshiki, I. Muramatsu. Identification of M1-muscarinic receptor subtype in rat stomach using a tissue segment binding method, and the effects of immobilization stress on the muscarinic receptors. Eur. J. Pharmacol. 599 (2008) 146–151.
A. Khatun, M. M. Rana, M. R. I. Khan, M. I. I. Wahed, M. A. Habib, M. N. Uddin, Z. S. Sathi, A. R. M. R. Amin, A. S. M. Anisuzzaman, Molecular mechanism formalin induced toxicity and management, American J. Life Sci. 3(2015) 85-92.
S. Cohen, D. J.-Deverts, W. J. Doyle, G. E. Miller, E. Frank, B. S. Rabin, and R. B. Turner. Chronic stress, glucocorticoid receptor resistance, inflammation, and disease risk. PNAS. 10 (2012) 1073.
S. Cohen, D. J-Deverts, G. E. Miller. Psychological stress and diseases. JAMA. 298(10) (2007) 1685-1687.
H. J. Zimmerman, L. B. Seeff, Enzymes in hepatic disease., Diagnostic Enzymology (1970).
A. J. Anderson, W. E. Bocklehurst, A. L. Wills, Evidence for the role of lysosomes in the formation of prostaglandins during carrageenan induced inflammation in rat. Pharmacol. Res. Comm. 3 (1971) 13-17.
R. S. Telang, S. Chatterjee, C. Varshneya, Study on analgesic and antiinflammatory avtivities of Vitex negunda Linn, Gen Pharmacol 31 (1990) 363-366.
E. M. Conner, M. B. Grisham, Inflammation, free radicals, and antioxidants Nutrition 12 (1996) 274-277.
D. I. Feig, D. H. Kang, R. J. Johnson. Uric acid and cardiovascular risk. N Engl J Med. 359 (2008) 1811–1821.
A. M. Fernandez-Leon, M. Lozano, D. Gonzalez, M. C. Ayuso, M. F. Fernandez-Leon. Bioactive Compounds Content and Total Antioxidant Activity of Two Savoy Cabbages. Czech J. Food Sci. 32 (2014) 549–554.
A. Gurel, O. Coskun, F. Armutcu, M. Kanter, O. A. Ozen, Vitamin E against oxidative damage caused by formaldehyde in frontal cortex and hippocampus: biochemical and histological studies. J Chem Neuroanat. 29 (2005) 173-178.
R. J. Verma, A. Nair, Amerliorative effect of Vitamin E on aflatoxin-induced lipid peroxidation in the testis of mice. Asian J Androl. 3 (2001) 217-221.