Prevalence and Antibiotic Resistance of Escherichia coli O157: H7 Serotype from Chicken Droppings Produced by Free - Ranged and Poultry Birds in Cross River, Nigeria
American Journal of Biomedical and Life Sciences
Volume 6, Issue 3, June 2018, Pages: 51-55
Received: May 23, 2018; Accepted: Jun. 7, 2018; Published: Jul. 21, 2018
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Authors
Nfongeh Joseph Fuh, Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria
Owoseni Mojisola Christiana, Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria
Adogo Lillian Yami, Department of Biological Sciences, Faculty of Science, Bingham University, Karu, Nigeria
Upla Peter Uteh, Department of Microbiology, Faculty of Science, Federal University Lafia, Lafia, Nigeria
Ekpiken Solomon Ekpiken, Department of Biological Sciences, Faculty of Science, Cross River University of Technology, Calabar, Nigeria
Uchenwa Mercy Ogechi, Department of Microbiology, Faculty of Science, University of Calabar, Calabar, Nigeria
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Abstract
This study investigated the prevalence of Escherichia coli O157:H7 from chicken droppings produced by free ranged and poultry birds at different locations within Cross River State, Nigeria and their susceptibility to commonly used antibiotics. A total of 360 cloacal swab samples each were randomly collected from poultry (confined) and free ranged (unconfined) chickens. Standard cultural, biochemical, and serological (latex agglutination) methods were used to isolate E. coli O157:H7. The isolates were subjected to antimicrobial susceptibility testing using disc diffusion method. Out of a total number of 360 anal swab samples collected from poultry and free-ranged chicken, 24 (6.67%) strayed and 7 (1.94%) poultry samples were positive for E. coli O157:H7 and the prevalence values differed significantly (p<0.05) among the group of birds. Values for age category among the free-ranged birds also differed significantly (p<0.05) with strayed chicks (1-3weeks old) having highest value of 10.89%. Out of the 9 isolates screened, 8 (88.89%) were resistant to tetracycline, 7 (77.78%) to ampicillin and nitrofurantoin and 6 (66.67%) to chloramphenicol. All isolates were resistant to at least one antibiotic. This study reveals that the intestinal track of chicken harbors the bacterial pathogen hence interventions are needed to reduce transmission of E. coli O157:H7 via poultry products.
Keywords
Escherichia coli, Poultry, Antibiotic Resistance, Poultry Droppings
To cite this article
Nfongeh Joseph Fuh, Owoseni Mojisola Christiana, Adogo Lillian Yami, Upla Peter Uteh, Ekpiken Solomon Ekpiken, Uchenwa Mercy Ogechi, Prevalence and Antibiotic Resistance of Escherichia coli O157: H7 Serotype from Chicken Droppings Produced by Free - Ranged and Poultry Birds in Cross River, Nigeria, American Journal of Biomedical and Life Sciences. Vol. 6, No. 3, 2018, pp. 51-55. doi: 10.11648/j.ajbls.20180603.13
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Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
C. P. Sousa “Escherichia coli as a specialized bacterial pathogen,” Revista De Biologia E Ciências Da Terra, vol. 6, pp. 341-349, 2006.
[2]
A. L. Ballou, R. A. Ali, M. A. Mendoza, J. C. Ellis, H. M. Hassan, and W. J. Croom, “Development of the chick microbiome: how early exposure influences future microbial diversity,” Front Vet Sci. vol 3, 2016, doi: 10.3389/fvets.2016.00002.
[3]
Y. Luo, S. Cui, J. Li, J. Yang, L. Lin, C. Hu, S. Jin, L. Ye, Q. Zhao, and Y. Ma, “Characterization of Escherichia coli isolates from healthy food handlers in hospital,” Microbial Drug Resistance, 17. pp. 443-448, 2011.
[4]
A. J. Stewardson, G. Renzi, N. Maury, C. Vaudaux, C. Brassier, E. Fritsch, D. Pittet, M. Heck, K. van der Zwaluw, and E. A. Reuland, “Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae in Hospital Food: A Risk Assessment,” Infection Control & Hospital Epidemiology, vol. 35, pp. 375-383, 2014.
[5]
J. T. Hemen, J. T. Johnson, E. E. Ambo, V. S. Ekam, M. O. Odey, and W. A. Fila,” Multi-antibiotic resistance of some gram negative bacterial isolates from poultry litters of selected farms in Benue State,” International Journal of Sciences and Technology, vol 2, pp. 543–547, 2012.
[6]
M. Agbaje, R. Davies, M. A. Oyekunle, O. E. Ojo, F. O. Fasina, and P. A. Akinduti, “Observation on the occurrence and transmission pattern of Salmonella Gallinarum in commercial poultry farms in Ogun State, South Western Nigeria,” African Journal Microbiological Research, vol 4, pp. 796-800, 2010.
[7]
L. H. Gould, K. A. Walsh, A. R. Vieira, K. Herman, I. T. Williams, A. J. Hall, and D. Cole, ''Surveillance for food borne disease Outbreaks-United States, 1998-2008'', MMWR Surveill. Summ., vol. 62, pp. 1-34, 2013.
[8]
A. H. Havelaar, M. D. Kirk, P. R. Torgerson, H. J. Gibb, T. Hald, R. J. Lake, N. Praet, D. C. Bellinger, N. R. De Silva, and N. Gargouri, “World Health Organization Global estimates and regional comparisons of the burden of food borne disease in 2010”, PLoS Med. 12. e1001923, 2015.
[9]
Z. Chen, and X. Jiang, “Microbiological safety of chicken litter or chicken litter-based organic fertilizers: a review,” Agriculture, vol. 4, pp. 1–29, 2014.
[10]
I. T. Mbata, Poultry meat pathogens and its Control. Internet Journal of Food Safety. 5 (7): 20-28, 2007.
[11]
W. Witte, “Medical consequences of antibiotic use in agriculture,” Science, 279, 996–7, 1998.
[12]
J. A. Odwar, G. Kikuvi, J. N. Kariuki, and S. Kariuki, “A cross-sectional study on the microbiological quality and safety of raw chicken meats sold in Nairobi, Kenya,” BMC Research. 7, article 627 doi: 10.1186/1756-0500-7-627, 2014.
[13]
National Bureau of Statistics. Annual Abstract of Statistics, 23, 2012.
[14]
D. G. Milley, and L. H. Sekia, “An Enzyme-linked Immunosorbent Assay-based isolation procedure for verotoxigenic E. coli.” Appl. and Environ Microbiol. vol. 59, pp. 4223-4229, 1993.
[15]
D. Warburton, and D. Christensen,” Isolation of E. coli O157: H7 or NM in foods, MFLP-80. Health Canada. The compendium of analytical methods,” Polyscience Publications, Laval, Quebec, Canada, 2006.
[16]
N. Pradel, V. Lirelli, C. Champs, J. B. Palcoux, A. Reynaud, F. Cheutz, F. J. Sirot, B. Joly, and C. Forestier, “Prevalence and characterization of Shiga-toxin producing Escherichia coli isolated from cattle, food and children during a one-year prospective study in France,” Journal of Clinical Microbiology, vol. 38, pp. 1023-1031, 2000.
[17]
Clinical and Laboratory Standards Institute, “Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacterial Isolated from Animals; Approved Standard,” Clinical and Laboratory Standards Institute, Wayne, PAUSA. Fourth Edition and Supplement, CLSI document VET01-A4 (standard) and VET01-S2 (supplement), 2013.
[18]
O. E. Ojo, M. A. Oyekunle, A. O. Ogunleye, and E. B. Otesile, “E. coli O157:H7 in food animals in part of S/Western Nigeria: prevalence and in vitro antimicrobial susceptibility,” Tropical Veterinarian, vol. 26, pp. 23–30, 2009.
[19]
I. O. Olatoye, E. A. Amosun, and G. A. T. Ogundipe, “Multidrug resistant Escherichia coli O157 contamination of beef and chicken in municipal abattoirs of Southwest, “ Nigeria Natural Sciences. vol. 10, pp. 125–132, 2012.
[20]
O. A. Kehinde, and O. O. Funmilola, “Antibiotic Usage Pattern in Poultry and Resistance Pattern of Human Pathogenic Bacteria Isolated from Poultry Droppings in Akure, Nigeria,” International Journal of Biomedical Science and Engineering, vol. 5, pp. 35-40, 2017a.
[21]
S. Mude, T. Naod, K. Jelalu, and M. Yimer, “Cloacael Carriage and Multidrug Resistance Escherichia coli O157:H7 from Poultry Farms, Eastern Ethiopia,” Journal of Veterinary Medicine. Article ID 8264583, 2017.
[22]
A. Chadran, and A. Mazumder, “Occurrence of Diarrheagenic Virulence Genes and Genetic diversity in Escherichia coli Isolates from feacal material of various Avian Hosts in British Columbia, Canada,” Appl. Environ. Microbio., vol 80, pp. 1933 – 1940, 2014.
[23]
X. Chen, W., Zhang, J. Yin, N. Zhang, S. Geng, and X. Zhou, “Escherichia coli isolates from sick chickens in China: Changes in antimicrobial resistance between 1993 and 2013,” Vet. Journ. vol. 202, pp. 112–115, 2014, pmid:25155303.
[24]
D. Hailu, and G. Tefera, “Isolation and characterization of multidrug resistant Escherichia coli isolates from contagion syndrome poultry farm,” Int. Journ. Cur. Tren. Pharmacobio. Med. Sci., vol. 1, pp. 19–26, 2016.
[25]
A. K, Yassin, J. Gong, P. Kelly, G. Lu,, L. Guardabassiand, L. Wei, “Antimicrobial resistance in clinical Escherichia coli isolates from poultry and livestock, China,” PLoS ONE. vol. 12, e01853262017.
[26]
O. A. Kehinde, and O. O. Funmilola, “Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria,” Biochem. and Mol. Bio., vol. 2, pp. 6-11, 2017b.
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