In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism

Mbah-Omeje; Kelechi Nkechinyere

doi:doi:10.11648/j.ajbls.20190701.11

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In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism

Mbah-Omeje, Kelechi Nkechinyere

Published in American Journal of Biomedical and Life Sciences (Volume 7, Issue 1)

Received: 23 November 2018 Accepted: 20 December 2018 Published: 15 February 2019

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Abstract

The present study investigates the antimicrobial activity, phytochemical and minimum inhibitory concentration (MIC) of Curcuma longa rhizome extract on Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus salivarius. Methanol, and chloroform extracts of the plant rhizome were collected and obtained by standard methods. All the solvent extracts were evaporated to dryness, dry residues were dissolved in dimethyl sulfoxide (DMSO) and tested for antibacterial activity. The plant extract was distinctively applied as antibacterial agent through agar well diffusion method on aseptically prepared nutrient agar. It was determined in the result of this study, thatthe chloroform extract of Curcuma longa rhizome was found to show more activity than the methanol extract on all the isolates. The inhibition zone diameter (IZD) of the chloroform extracts ranged between 7-34mm while ethanol extract ranged between 3-13mm. The MIC varied between 1.56 - 3.125mg/ml and 1.56 -6.25mg/ml for methanol and chloroform extract respectively while Staphylococcus epidermid is showed the least sensitivity of all the isolates The chloroform extracts exhibited higher inhibitory activity on the test organisms than the positive control ciprofloxacin. Phytochemical analyses of the extracts revealed the presence of alkaloids, tannins, phenolic compounds, terpenoids, saponins and flavonoids. According to this study, Curcuma longa rhizome can be used for the treatment of diseases caused by Staphylococcus sppas well as Streptococcus salivarius.

Published in	American Journal of Biomedical and Life Sciences (Volume 7, Issue 1)
DOI	10.11648/j.ajbls.20190701.11
Page(s)	1-5
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Curcuma Longa, Staphylococcus Aureus, Staphylococcus Epidermidis, Streptococcus Salivarius, Extracts

References

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APA Style

Mbah-Omeje, Kelechi Nkechinyere. (2019). In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism. American Journal of Biomedical and Life Sciences, 7(1), 1-5. https://doi.org/10.11648/j.ajbls.20190701.11

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ACS Style

Mbah-Omeje; Kelechi Nkechinyere. In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism. Am. J. Biomed. Life Sci. 2019, 7(1), 1-5. doi: 10.11648/j.ajbls.20190701.11

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AMA Style

Mbah-Omeje, Kelechi Nkechinyere. In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism. Am J Biomed Life Sci. 2019;7(1):1-5. doi: 10.11648/j.ajbls.20190701.11

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@article{10.11648/j.ajbls.20190701.11,
  author = {Mbah-Omeje and Kelechi Nkechinyere},
  title = {In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {7},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.ajbls.20190701.11},
  url = {https://doi.org/10.11648/j.ajbls.20190701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20190701.11},
  abstract = {The present study investigates the antimicrobial activity, phytochemical and minimum inhibitory concentration (MIC) of Curcuma longa rhizome extract on Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus salivarius. Methanol, and chloroform extracts of the plant rhizome were collected and obtained by standard methods. All the solvent extracts were evaporated to dryness, dry residues were dissolved in dimethyl sulfoxide (DMSO) and tested for antibacterial activity. The plant extract was distinctively applied as antibacterial agent through agar well diffusion method on aseptically prepared nutrient agar. It was determined in the result of this study, thatthe chloroform extract of Curcuma longa rhizome was found to show more activity than the methanol extract on all the isolates. The inhibition zone diameter (IZD) of the chloroform extracts ranged between 7-34mm while ethanol extract ranged between 3-13mm. The MIC varied between 1.56 - 3.125mg/ml and 1.56 -6.25mg/ml for methanol and chloroform extract respectively while Staphylococcus epidermid is showed the least sensitivity of all the isolates The chloroform extracts exhibited higher inhibitory activity on the test organisms than the positive control ciprofloxacin. Phytochemical analyses of the extracts revealed the presence of alkaloids, tannins, phenolic compounds, terpenoids, saponins and flavonoids. According to this study, Curcuma longa rhizome can be used for the treatment of diseases caused by Staphylococcus sppas well as Streptococcus salivarius.},
 year = {2019}
}

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TY  - JOUR
T1  - In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism
AU  - Mbah-Omeje
AU  - Kelechi Nkechinyere
Y1  - 2019/02/15
PY  - 2019
N1  - https://doi.org/10.11648/j.ajbls.20190701.11
DO  - 10.11648/j.ajbls.20190701.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 1
EP  - 5
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20190701.11
AB  - The present study investigates the antimicrobial activity, phytochemical and minimum inhibitory concentration (MIC) of Curcuma longa rhizome extract on Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus salivarius. Methanol, and chloroform extracts of the plant rhizome were collected and obtained by standard methods. All the solvent extracts were evaporated to dryness, dry residues were dissolved in dimethyl sulfoxide (DMSO) and tested for antibacterial activity. The plant extract was distinctively applied as antibacterial agent through agar well diffusion method on aseptically prepared nutrient agar. It was determined in the result of this study, thatthe chloroform extract of Curcuma longa rhizome was found to show more activity than the methanol extract on all the isolates. The inhibition zone diameter (IZD) of the chloroform extracts ranged between 7-34mm while ethanol extract ranged between 3-13mm. The MIC varied between 1.56 - 3.125mg/ml and 1.56 -6.25mg/ml for methanol and chloroform extract respectively while Staphylococcus epidermid is showed the least sensitivity of all the isolates The chloroform extracts exhibited higher inhibitory activity on the test organisms than the positive control ciprofloxacin. Phytochemical analyses of the extracts revealed the presence of alkaloids, tannins, phenolic compounds, terpenoids, saponins and flavonoids. According to this study, Curcuma longa rhizome can be used for the treatment of diseases caused by Staphylococcus sppas well as Streptococcus salivarius.
VL  - 7
IS  - 1
ER  -

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Author Information

Mbah-Omeje

Department of Applied Microbiology and Brewing, Enugu State University of Science and Technology, Enugu State, Nigeria
Kelechi Nkechinyere

Department of Applied Microbiology and Brewing, Enugu State University of Science and Technology, Enugu State, Nigeria

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APA Style

Mbah-Omeje, Kelechi Nkechinyere. (2019). In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism. American Journal of Biomedical and Life Sciences, 7(1), 1-5. https://doi.org/10.11648/j.ajbls.20190701.11

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ACS Style

Mbah-Omeje; Kelechi Nkechinyere. In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism. Am. J. Biomed. Life Sci. 2019, 7(1), 1-5. doi: 10.11648/j.ajbls.20190701.11

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AMA Style

Mbah-Omeje, Kelechi Nkechinyere. In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism. Am J Biomed Life Sci. 2019;7(1):1-5. doi: 10.11648/j.ajbls.20190701.11

Copy | Download

@article{10.11648/j.ajbls.20190701.11,
  author = {Mbah-Omeje and Kelechi Nkechinyere},
  title = {In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {7},
  number = {1},
  pages = {1-5},
  doi = {10.11648/j.ajbls.20190701.11},
  url = {https://doi.org/10.11648/j.ajbls.20190701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20190701.11},
  abstract = {The present study investigates the antimicrobial activity, phytochemical and minimum inhibitory concentration (MIC) of Curcuma longa rhizome extract on Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus salivarius. Methanol, and chloroform extracts of the plant rhizome were collected and obtained by standard methods. All the solvent extracts were evaporated to dryness, dry residues were dissolved in dimethyl sulfoxide (DMSO) and tested for antibacterial activity. The plant extract was distinctively applied as antibacterial agent through agar well diffusion method on aseptically prepared nutrient agar. It was determined in the result of this study, thatthe chloroform extract of Curcuma longa rhizome was found to show more activity than the methanol extract on all the isolates. The inhibition zone diameter (IZD) of the chloroform extracts ranged between 7-34mm while ethanol extract ranged between 3-13mm. The MIC varied between 1.56 - 3.125mg/ml and 1.56 -6.25mg/ml for methanol and chloroform extract respectively while Staphylococcus epidermid is showed the least sensitivity of all the isolates The chloroform extracts exhibited higher inhibitory activity on the test organisms than the positive control ciprofloxacin. Phytochemical analyses of the extracts revealed the presence of alkaloids, tannins, phenolic compounds, terpenoids, saponins and flavonoids. According to this study, Curcuma longa rhizome can be used for the treatment of diseases caused by Staphylococcus sppas well as Streptococcus salivarius.},
 year = {2019}
}

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TY  - JOUR
T1  - In Vitro Study on the Antimicrobial Activity of Curcuma Longa Rhizome on Some Microorganism
AU  - Mbah-Omeje
AU  - Kelechi Nkechinyere
Y1  - 2019/02/15
PY  - 2019
N1  - https://doi.org/10.11648/j.ajbls.20190701.11
DO  - 10.11648/j.ajbls.20190701.11
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 1
EP  - 5
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20190701.11
AB  - The present study investigates the antimicrobial activity, phytochemical and minimum inhibitory concentration (MIC) of Curcuma longa rhizome extract on Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus salivarius. Methanol, and chloroform extracts of the plant rhizome were collected and obtained by standard methods. All the solvent extracts were evaporated to dryness, dry residues were dissolved in dimethyl sulfoxide (DMSO) and tested for antibacterial activity. The plant extract was distinctively applied as antibacterial agent through agar well diffusion method on aseptically prepared nutrient agar. It was determined in the result of this study, thatthe chloroform extract of Curcuma longa rhizome was found to show more activity than the methanol extract on all the isolates. The inhibition zone diameter (IZD) of the chloroform extracts ranged between 7-34mm while ethanol extract ranged between 3-13mm. The MIC varied between 1.56 - 3.125mg/ml and 1.56 -6.25mg/ml for methanol and chloroform extract respectively while Staphylococcus epidermid is showed the least sensitivity of all the isolates The chloroform extracts exhibited higher inhibitory activity on the test organisms than the positive control ciprofloxacin. Phytochemical analyses of the extracts revealed the presence of alkaloids, tannins, phenolic compounds, terpenoids, saponins and flavonoids. According to this study, Curcuma longa rhizome can be used for the treatment of diseases caused by Staphylococcus sppas well as Streptococcus salivarius.
VL  - 7
IS  - 1
ER  -

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