Investigation of Antibacterial Activity of Crude Extracts from Marine Snails and Bivalves in the Southern Coast of Vietnam

Pham Xuan Ky; Pham Thi Mien; Le Ho Khanh Hy; Dao Viet Ha; Nguyen Phuong Anh; Doan Thi Thiet; Phan Bao Vy; Ho Van The

doi:doi:10.11648/j.ajbls.20190701.13

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Investigation of Antibacterial Activity of Crude Extracts from Marine Snails and Bivalves in the Southern Coast of Vietnam

Pham Xuan Ky, Pham Thi Mien, Le Ho Khanh Hy, Dao Viet Ha, Nguyen Phuong Anh, Doan Thi Thiet, Phan Bao Vy, Ho Van The

Published in American Journal of Biomedical and Life Sciences (Volume 7, Issue 1)

Received: 12 February 2019 Accepted: 14 March 2019 Published: 10 April 2019

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Abstract

The primary antibacterial activity of methanol and chloroform crude extracts from marine snails and bivalves was assessed by using the agar diffusion technique against four bacterial strains. Active methanol extracts were then characterized using TLC, SDS-PAGE and FTIR. Methanol extracts from 5 snail species and 8 extracts from 12 bivalve species possessed the ability to inhibit Bacillus subtilis. Methanol extracts from 3 snail species Tectus conus, Maninella alounia and Trochus maculatus inhibited Escheria coli and those from 4 snail species Cerithium chinatum, Maninella alounia, Tectus pyramis, Trochus maculatus and the bivalve species Pinna bicolor exhibited activity against Serratia marcescens. Chloroform extracts from 7 snail species and those from 7 bivalve species showed inhibition on Bacillus subtilis. Only chloroform extract from the bivalve Chama cf dunkeri was active on Salmonella typhimur and that from the snail Trochus maculatus and bivalve Lopha cristagali inhibited Escheria coli. TLC and FTIR analysis of active methanol extracts showed the presence of amino acids, peptides and proteins. SDS-PAGE of those extracts also revealed proteins with a molecular weight range between 10 and 28 kDa. The obtained results indicate the potential antimicrobial compounds that could be explored in snail and bivalve in Vietnam.

Published in	American Journal of Biomedical and Life Sciences (Volume 7, Issue 1)
DOI	10.11648/j.ajbls.20190701.13
Page(s)	10-15
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Snail, Bivalve, Crude Extracts, Antibacterial Activity, TLC, FTIR, SDS-PAGE

References

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APA Style

Pham Xuan Ky, Pham Thi Mien, Le Ho Khanh Hy, Dao Viet Ha, Nguyen Phuong Anh, et al. (2019). Investigation of Antibacterial Activity of Crude Extracts from Marine Snails and Bivalves in the Southern Coast of Vietnam. American Journal of Biomedical and Life Sciences, 7(1), 10-15. https://doi.org/10.11648/j.ajbls.20190701.13

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ACS Style

Pham Xuan Ky; Pham Thi Mien; Le Ho Khanh Hy; Dao Viet Ha; Nguyen Phuong Anh, et al. Investigation of Antibacterial Activity of Crude Extracts from Marine Snails and Bivalves in the Southern Coast of Vietnam. Am. J. Biomed. Life Sci. 2019, 7(1), 10-15. doi: 10.11648/j.ajbls.20190701.13

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AMA Style

Pham Xuan Ky, Pham Thi Mien, Le Ho Khanh Hy, Dao Viet Ha, Nguyen Phuong Anh, et al. Investigation of Antibacterial Activity of Crude Extracts from Marine Snails and Bivalves in the Southern Coast of Vietnam. Am J Biomed Life Sci. 2019;7(1):10-15. doi: 10.11648/j.ajbls.20190701.13

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@article{10.11648/j.ajbls.20190701.13,
  author = {Pham Xuan Ky and Pham Thi Mien and Le Ho Khanh Hy and Dao Viet Ha and Nguyen Phuong Anh and Doan Thi Thiet and Phan Bao Vy and Ho Van The},
  title = {Investigation of Antibacterial Activity of Crude Extracts from Marine Snails and Bivalves in the Southern Coast of Vietnam},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {7},
  number = {1},
  pages = {10-15},
  doi = {10.11648/j.ajbls.20190701.13},
  url = {https://doi.org/10.11648/j.ajbls.20190701.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20190701.13},
  abstract = {The primary antibacterial activity of methanol and chloroform crude extracts from marine snails and bivalves was assessed by using the agar diffusion technique against four bacterial strains. Active methanol extracts were then characterized using TLC, SDS-PAGE and FTIR. Methanol extracts from 5 snail species and 8 extracts from 12 bivalve species possessed the ability to inhibit Bacillus subtilis. Methanol extracts from 3 snail species Tectus conus, Maninella alounia and Trochus maculatus inhibited Escheria coli and those from 4 snail species Cerithium chinatum, Maninella alounia, Tectus pyramis, Trochus maculatus and the bivalve species Pinna bicolor exhibited activity against Serratia marcescens. Chloroform extracts from 7 snail species and those from 7 bivalve species showed inhibition on Bacillus subtilis. Only chloroform extract from the bivalve Chama cf dunkeri was active on Salmonella typhimur and that from the snail Trochus maculatus and bivalve Lopha cristagali inhibited Escheria coli. TLC and FTIR analysis of active methanol extracts showed the presence of amino acids, peptides and proteins. SDS-PAGE of those extracts also revealed proteins with a molecular weight range between 10 and 28 kDa. The obtained results indicate the potential antimicrobial compounds that could be explored in snail and bivalve in Vietnam.},
 year = {2019}
}

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TY  - JOUR
T1  - Investigation of Antibacterial Activity of Crude Extracts from Marine Snails and Bivalves in the Southern Coast of Vietnam
AU  - Pham Xuan Ky
AU  - Pham Thi Mien
AU  - Le Ho Khanh Hy
AU  - Dao Viet Ha
AU  - Nguyen Phuong Anh
AU  - Doan Thi Thiet
AU  - Phan Bao Vy
AU  - Ho Van The
Y1  - 2019/04/10
PY  - 2019
N1  - https://doi.org/10.11648/j.ajbls.20190701.13
DO  - 10.11648/j.ajbls.20190701.13
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 10
EP  - 15
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20190701.13
AB  - The primary antibacterial activity of methanol and chloroform crude extracts from marine snails and bivalves was assessed by using the agar diffusion technique against four bacterial strains. Active methanol extracts were then characterized using TLC, SDS-PAGE and FTIR. Methanol extracts from 5 snail species and 8 extracts from 12 bivalve species possessed the ability to inhibit Bacillus subtilis. Methanol extracts from 3 snail species Tectus conus, Maninella alounia and Trochus maculatus inhibited Escheria coli and those from 4 snail species Cerithium chinatum, Maninella alounia, Tectus pyramis, Trochus maculatus and the bivalve species Pinna bicolor exhibited activity against Serratia marcescens. Chloroform extracts from 7 snail species and those from 7 bivalve species showed inhibition on Bacillus subtilis. Only chloroform extract from the bivalve Chama cf dunkeri was active on Salmonella typhimur and that from the snail Trochus maculatus and bivalve Lopha cristagali inhibited Escheria coli. TLC and FTIR analysis of active methanol extracts showed the presence of amino acids, peptides and proteins. SDS-PAGE of those extracts also revealed proteins with a molecular weight range between 10 and 28 kDa. The obtained results indicate the potential antimicrobial compounds that could be explored in snail and bivalve in Vietnam.
VL  - 7
IS  - 1
ER  -

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Author Information

Pham Xuan Ky

Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Pham Thi Mien

Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Le Ho Khanh Hy

Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Dao Viet Ha

Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Nguyen Phuong Anh

Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Doan Thi Thiet

Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Phan Bao Vy

Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Ho Van The

Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam

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APA Style

Pham Xuan Ky, Pham Thi Mien, Le Ho Khanh Hy, Dao Viet Ha, Nguyen Phuong Anh, et al. (2019). Investigation of Antibacterial Activity of Crude Extracts from Marine Snails and Bivalves in the Southern Coast of Vietnam. American Journal of Biomedical and Life Sciences, 7(1), 10-15. https://doi.org/10.11648/j.ajbls.20190701.13

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ACS Style

Pham Xuan Ky; Pham Thi Mien; Le Ho Khanh Hy; Dao Viet Ha; Nguyen Phuong Anh, et al. Investigation of Antibacterial Activity of Crude Extracts from Marine Snails and Bivalves in the Southern Coast of Vietnam. Am. J. Biomed. Life Sci. 2019, 7(1), 10-15. doi: 10.11648/j.ajbls.20190701.13

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AMA Style

Pham Xuan Ky, Pham Thi Mien, Le Ho Khanh Hy, Dao Viet Ha, Nguyen Phuong Anh, et al. Investigation of Antibacterial Activity of Crude Extracts from Marine Snails and Bivalves in the Southern Coast of Vietnam. Am J Biomed Life Sci. 2019;7(1):10-15. doi: 10.11648/j.ajbls.20190701.13

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@article{10.11648/j.ajbls.20190701.13,
  author = {Pham Xuan Ky and Pham Thi Mien and Le Ho Khanh Hy and Dao Viet Ha and Nguyen Phuong Anh and Doan Thi Thiet and Phan Bao Vy and Ho Van The},
  title = {Investigation of Antibacterial Activity of Crude Extracts from Marine Snails and Bivalves in the Southern Coast of Vietnam},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {7},
  number = {1},
  pages = {10-15},
  doi = {10.11648/j.ajbls.20190701.13},
  url = {https://doi.org/10.11648/j.ajbls.20190701.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20190701.13},
  abstract = {The primary antibacterial activity of methanol and chloroform crude extracts from marine snails and bivalves was assessed by using the agar diffusion technique against four bacterial strains. Active methanol extracts were then characterized using TLC, SDS-PAGE and FTIR. Methanol extracts from 5 snail species and 8 extracts from 12 bivalve species possessed the ability to inhibit Bacillus subtilis. Methanol extracts from 3 snail species Tectus conus, Maninella alounia and Trochus maculatus inhibited Escheria coli and those from 4 snail species Cerithium chinatum, Maninella alounia, Tectus pyramis, Trochus maculatus and the bivalve species Pinna bicolor exhibited activity against Serratia marcescens. Chloroform extracts from 7 snail species and those from 7 bivalve species showed inhibition on Bacillus subtilis. Only chloroform extract from the bivalve Chama cf dunkeri was active on Salmonella typhimur and that from the snail Trochus maculatus and bivalve Lopha cristagali inhibited Escheria coli. TLC and FTIR analysis of active methanol extracts showed the presence of amino acids, peptides and proteins. SDS-PAGE of those extracts also revealed proteins with a molecular weight range between 10 and 28 kDa. The obtained results indicate the potential antimicrobial compounds that could be explored in snail and bivalve in Vietnam.},
 year = {2019}
}

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TY  - JOUR
T1  - Investigation of Antibacterial Activity of Crude Extracts from Marine Snails and Bivalves in the Southern Coast of Vietnam
AU  - Pham Xuan Ky
AU  - Pham Thi Mien
AU  - Le Ho Khanh Hy
AU  - Dao Viet Ha
AU  - Nguyen Phuong Anh
AU  - Doan Thi Thiet
AU  - Phan Bao Vy
AU  - Ho Van The
Y1  - 2019/04/10
PY  - 2019
N1  - https://doi.org/10.11648/j.ajbls.20190701.13
DO  - 10.11648/j.ajbls.20190701.13
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 10
EP  - 15
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20190701.13
AB  - The primary antibacterial activity of methanol and chloroform crude extracts from marine snails and bivalves was assessed by using the agar diffusion technique against four bacterial strains. Active methanol extracts were then characterized using TLC, SDS-PAGE and FTIR. Methanol extracts from 5 snail species and 8 extracts from 12 bivalve species possessed the ability to inhibit Bacillus subtilis. Methanol extracts from 3 snail species Tectus conus, Maninella alounia and Trochus maculatus inhibited Escheria coli and those from 4 snail species Cerithium chinatum, Maninella alounia, Tectus pyramis, Trochus maculatus and the bivalve species Pinna bicolor exhibited activity against Serratia marcescens. Chloroform extracts from 7 snail species and those from 7 bivalve species showed inhibition on Bacillus subtilis. Only chloroform extract from the bivalve Chama cf dunkeri was active on Salmonella typhimur and that from the snail Trochus maculatus and bivalve Lopha cristagali inhibited Escheria coli. TLC and FTIR analysis of active methanol extracts showed the presence of amino acids, peptides and proteins. SDS-PAGE of those extracts also revealed proteins with a molecular weight range between 10 and 28 kDa. The obtained results indicate the potential antimicrobial compounds that could be explored in snail and bivalve in Vietnam.
VL  - 7
IS  - 1
ER  -

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