Investigation of Antibacterial Activity of Crude Extracts from Marine Snails and Bivalves in the Southern Coast of Vietnam
American Journal of Biomedical and Life Sciences
Volume 7, Issue 1, February 2019, Pages: 10-15
Received: Feb. 12, 2019;
Accepted: Mar. 14, 2019;
Published: Apr. 10, 2019
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Pham Xuan Ky, Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Pham Thi Mien, Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Le Ho Khanh Hy, Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Dao Viet Ha, Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Nguyen Phuong Anh, Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Doan Thi Thiet, Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Phan Bao Vy, Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
Ho Van The, Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), Nha Trang, Khanh Hoa, Vietnam
The primary antibacterial activity of methanol and chloroform crude extracts from marine snails and bivalves was assessed by using the agar diffusion technique against four bacterial strains. Active methanol extracts were then characterized using TLC, SDS-PAGE and FTIR. Methanol extracts from 5 snail species and 8 extracts from 12 bivalve species possessed the ability to inhibit Bacillus subtilis. Methanol extracts from 3 snail species Tectus conus, Maninella alounia and Trochus maculatus inhibited Escheria coli and those from 4 snail species Cerithium chinatum, Maninella alounia, Tectus pyramis, Trochus maculatus and the bivalve species Pinna bicolor exhibited activity against Serratia marcescens. Chloroform extracts from 7 snail species and those from 7 bivalve species showed inhibition on Bacillus subtilis. Only chloroform extract from the bivalve Chama cf dunkeri was active on Salmonella typhimur and that from the snail Trochus maculatus and bivalve Lopha cristagali inhibited Escheria coli. TLC and FTIR analysis of active methanol extracts showed the presence of amino acids, peptides and proteins. SDS-PAGE of those extracts also revealed proteins with a molecular weight range between 10 and 28 kDa. The obtained results indicate the potential antimicrobial compounds that could be explored in snail and bivalve in Vietnam.
Pham Xuan Ky,
Pham Thi Mien,
Le Ho Khanh Hy,
Dao Viet Ha,
Nguyen Phuong Anh,
Doan Thi Thiet,
Phan Bao Vy,
Ho Van The,
Investigation of Antibacterial Activity of Crude Extracts from Marine Snails and Bivalves in the Southern Coast of Vietnam, American Journal of Biomedical and Life Sciences.
Vol. 7, No. 1,
2019, pp. 10-15.
Amornrut C., Toida T., Imanari T., Woo E. R., Park H., Linhardt R., Wu S. J. and Kim Y. S. (1999). A new sulfated beta-galactan from clams with anti-HIV activity. Carbohydrate Research 321: 121–127.
Anderson R. S. and Beaven A. E. (2001). Antibacterial activities of oyster (Crassostrea virginica) mussel (Mytilus edulis and Geukensia demissa) plasma. Aquatic Living Resources 14: 343–49.
Barth A. (2007). Infrared spectroscopy of proteins. Biochimica et Biophysica Acta (BBA) – Bioenergetics. 1767, pp. 1073–1101.
Bauer A. W., Kirby W. M., Sherris J. C. and Turck M. (1996). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 45: 493-496.
Benkendorff K., Bremner J. B. and Davis A. R. (2001). Indole derivatives from the egg masses of Muricid molluscs. Molecules 6: 70–78.
Chandran B., Rameshkumar G. and Ravichandran S. (2009). Antimicrobial activity from the gill extraction of Perna viridis (Linnaeus, 1758). Global Journal of Biochemistry and Biotechnology 4: 88–92.
Chatterji A., Ansari Z. A., Ingole B. S., Bichurina M. A., Sovetova M. and Boikov Y. A. (2002). Indian marine bivalves: Potential source of antiviral drugs. Current Science 81: 1279–1282.
Cimino G., Fontana A., Cutignano A. and Gavagnin M. (2004). Biosynthesis in opisthobranch molluscs: General outline in the light of recent use of stable isotopes. Phytochemistry Reviews 3: 285–307.
Dang V. T., Kirsten B., Tim G. and Peter S. (2015). Marine snails and slugs: a great place to look for antiviral drugs. Journal of Virology 89: 8114–8118.
Degiam Z. D. and Abas A. T. (2010). Antimicrobial activity of some crude marine Mollusca extracts against some human pathogenic bacteria. Thi-Qar Medical Journal 3: 142–147.
Hubert F., Knaap W., Noël T. and Roch P. (1996). Cytotoxic and antibacterial properties of Mytilus gallofwozvincialis, Ostrea edulk and Crassostrea gigas (Bivalve molluscs) hemolymph. Aquatic Living Resources 9: 115–124.
Jayaseeli A. A., Anand P. and Murugan T. A. (2001). Antibacterial activity of four-bivalves from Gulf of Mannar. Phuket Mar. Biol. Cent. Spl. Pub. 25: 215–217.
Kanagasabapathy S., Samuthirapandian R. and Kumaresan M. (2011). Preliminary studies for a new antibiotic from the marine mollusk Melo melo (Lightfoot, 1786). Asian Pacific Journal of Tropical Medicine 4: 310–314.
Kiran N., Siddiqui G., Khan A. N., Ibrar K. and Tushar P. (2014). Extraction and screening of bioactive compounds with antimicrobial properties from selected species of mollusk and crustacean. Journal of Clinical & Cellular Immunology. doi.org/10.4172/2155-9899.1000189.
Laemmli U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature 227 : 680–685.
Madhu V. N., Sivaperumal P., Kamala K., Ambekar A. A. and Kulkarni B. G. (2014). Antibacterial and antioxidant activities of the tissue extract of Perna viridis Linnaeus, 1758 (mollusca: Bivalvia) from Versova coast, Mumbai. International Journal of Pharmacy and Pharmaceutical Sciences 6: 704–707.
Mitta G., Hubert F., Noel T. and Roch P. (1999). Myticin, a novel cysteine-rich antimicrobial peptide isolated from haemocytes and plasma of the mussel Mytilus galloprovincialis. European Journal of Biochemistry 265: 71–78.
Periyasamy N., Arularasan S. and Gayathri S. (2012a). Antibacterial activity of the tissue extracts of Conus betulinus and Conus inscriptus Linnaeus, 1758 (Mollusca: Gastropoda) from Nagapattinam, Southeast coast of India. Asian Pacific Journal of Tropical Biomedicine 2: 914–919.
Periyasamy N., Srinivasan M. and Balakrishnan S. (2012b). Antimicrobial activities of the tissue extracts of Babylonia spirata Linnaeus, 1758 (Mollusca: Gastropoda) from Thazhanguda, southeast coast of India. Asian Pacific Journal of Tropical Biomedicine 2: 36–40.
Phan T. T. H. and Chau V. M. (2016). Study on cytotoxicity, antimicrobial activity and search for bioactive coumpounds from 10 mollusc species in Cat Ba island. VAST Project No. 04.08/14–15.
Ramasamy P., Vino A. B., Saravanan R., Subhapradha N., Shanmugam V. and Shanmugam A. (2011). Screening of antimicrobial potential of polysaccharide from cuttle bone and methanolic extract from body tissue of Sepia prashadi Winkworth, 1936. Asian Pacific Journal of Tropical Biomedicine 1: 244–248.
Ramya M. S., Sivasubramanian K., Ravichandran S. and Anbuchezhian R. (2014). Screening of antimicrobial compound from the sea slug Armina babai. Bangladesh Journal of Physiology and Pharmacology 9: 268–274.
Schroeder C. I., Smythe M. L. and Lewis R. J. (2004). Development of small molecules that mimic the binding of omega-conotoxins at the N-type voltagegated calcium channel. Molecular Diversity 8: 127–134.
Scotti P. D., Dearing S. C., Greenwood D. R. and Newcomb R. D. (2001). Pernin: a novel, self-aggregating haemolymph protein from the New Zealand greenlipped mussel, Perna canaliculus (Bivalvia: Mytilidae). Comparative Biochemistry & Physiology 128: 767–79.
Sumita S., Chatterji A. and Das P. (2009). Effect of different extraction procedures on antimicrobial activity of marine bivalves: a comparison. Pertanika Journal of Tropical Agricultural Science 32: 77–83.
Vennila R., Kumar R. K., Kanchana S., Arumugam M. and Balasubramanian T. (2011). Investigation of antimicrobial and plasma coagulation property of some molluscan ink extracts: Gastropods and cephalopods. African Journal of Biochemistry Research 5: 14–21.