Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis

Xi Qinhua; Teng Yajie; Li Yueqin; Dai Juan; Zhang Guangbo; Chen Weichang

doi:doi:10.11648/j.ajbls.20190706.13

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Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis

Xi Qinhua, Teng Yajie, Li Yueqin, Dai Juan, Zhang Guangbo, Chen Weichang

Published in American Journal of Biomedical and Life Sciences (Volume 7, Issue 6)

Received: 14 September 2019 Accepted: 29 October 2019 Published: 13 November 2019

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Abstract

This study was performed to study the effects of astragalus polysaccharides (APS) in the treatment of ulcerative colitis (UC), and to explore whether myeloid-derived suppressor cells (MDSC) involve in this process. UC model was established by dextran sulfate sodium salt (DSS) inducement in mouse. Then the effects of APS on UC was evaluate at the levels of cytology and molecular biology: exploring the influence of APS on MDSC by analyzing the levels of MDSC before and after the treatments of APS; evaluating the therapeutic effects of APS on UC by pathologic histology. Data showed that the levels of MDSC in bone marrow, spleen, peripheral blood of UC mice were significantly decreased after intervention with APS, indicating APS inhibited the level of MDSC significantly. After depletion bone marrow cells in mice, we further found that the therapeutic effects of APS in MDSC- group was significantly reduced compared with MDSC+ group. It was discovered through pathological analysis that, compared with UC model group, APS intervention group mainly manifested as reduced infiltrating acute and chronic inflammatory cells, necrotic epithelial cells and epithelial ulceration. Therefore, APS could reduce the inflammatory cell infiltration in colonic tissues of UC model mice, repair the damaged colonic mucosa, and promote ulcer healing. In conclusion, APS has a potential application in the treatment of ulcerative colitis, and is dependent or partially dependent on MDSC to achieve this effects.

Published in	American Journal of Biomedical and Life Sciences (Volume 7, Issue 6) This article belongs to the Special Issue Inflammatory Bowel Disease
DOI	10.11648/j.ajbls.20190706.13
Page(s)	143-147
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Astragalus Polysaccharides, Ulcerative Colitis, Mouse Model, MDSC

References

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[8]	Xia Y, Qin ZR, Shi YM. Therapeutic effect observation on 30 cases of ulcerative colitis treated with internal and external treatment [J]. Shanghai Journal of Traditional Chinese Medicine, 2000, 46 (10): 24.
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[11]	Han W, Liu ZQ, Sun L. Clinical research of using Salvia miltiorrhiza and astragalus injection in treating ulcerative colitis [J]. Journal of Changchun College of Traditional Chinese Medicine, 2005, 21 (4): 7.
[12]	Xiang J, Wang YB, Xu T, et al. Effect of astragalus polysaccharides on the host resistance to Listeria bacteria [J]. Journal of Wuhan University: Medical edition, 2007, 28 (6): 741-743.
[13]	Han JC, Yu YS, Tang ZH, et al. Effect of astragalus polysaccharides on the differentiation and maturation of mouse dendritic cells [J]. Shanghai Medicine, 2006, 29 (12): 873-876.
[14]	An SL, Zhang SY, Pu HS. Effect of astragalus polysaccharides in annual astragalus on mouse immune organ index [J]. Medical Journal of Yanbian University, 2007, 30 (1): 20-22.
[15]	Chen W, Li YM, Yu MH, et al. Immune regulatory effect of astragalus polysaccharides on the T-cell subset in diabetic mice [J]. China Journal of Modern Medicine, 2007, 17 (1): 28-31.
[16]	Zhao LH, Ma ZX, Zhu J, et al. Characterization of polysaccharides from astragalus radix as the macrophage stimulator [J]. Cellular Immunol, 2011, 271 (2): 329-334.
[17]	Zang KH, LI YD, ZHU LJ, et al. Study on the repair effect on colon mucosa and mechanism of astragalus polysaccharides in rats with ulcerative colitis [J]. GANSU UNIVERSITY OF CHINESE MEDICINE, 2018, 035 (003): P. 5-10.
[18]	Lv J, Zhang Y, Tian Z, Liu Y, Liu F, Shi Y, et al. Astragalus polysaccharides protect against dextran sulfate sodium-induced colitis by inhibiting NF-κB activation. International Journal of Biological Macromolecules. 2017; 98: 723-9.

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APA Style

Xi Qinhua, Teng Yajie, Li Yueqin, Dai Juan, Zhang Guangbo, et al. (2019). Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis. American Journal of Biomedical and Life Sciences, 7(6), 143-147. https://doi.org/10.11648/j.ajbls.20190706.13

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ACS Style

Xi Qinhua; Teng Yajie; Li Yueqin; Dai Juan; Zhang Guangbo, et al. Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis. Am. J. Biomed. Life Sci. 2019, 7(6), 143-147. doi: 10.11648/j.ajbls.20190706.13

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AMA Style

Xi Qinhua, Teng Yajie, Li Yueqin, Dai Juan, Zhang Guangbo, et al. Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis. Am J Biomed Life Sci. 2019;7(6):143-147. doi: 10.11648/j.ajbls.20190706.13

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@article{10.11648/j.ajbls.20190706.13,
  author = {Xi Qinhua and Teng Yajie and Li Yueqin and Dai Juan and Zhang Guangbo and Chen Weichang},
  title = {Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {7},
  number = {6},
  pages = {143-147},
  doi = {10.11648/j.ajbls.20190706.13},
  url = {https://doi.org/10.11648/j.ajbls.20190706.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20190706.13},
  abstract = {This study was performed to study the effects of astragalus polysaccharides (APS) in the treatment of ulcerative colitis (UC), and to explore whether myeloid-derived suppressor cells (MDSC) involve in this process. UC model was established by dextran sulfate sodium salt (DSS) inducement in mouse. Then the effects of APS on UC was evaluate at the levels of cytology and molecular biology: exploring the influence of APS on MDSC by analyzing the levels of MDSC before and after the treatments of APS; evaluating the therapeutic effects of APS on UC by pathologic histology. Data showed that the levels of MDSC in bone marrow, spleen, peripheral blood of UC mice were significantly decreased after intervention with APS, indicating APS inhibited the level of MDSC significantly. After depletion bone marrow cells in mice, we further found that the therapeutic effects of APS in MDSC- group was significantly reduced compared with MDSC+ group. It was discovered through pathological analysis that, compared with UC model group, APS intervention group mainly manifested as reduced infiltrating acute and chronic inflammatory cells, necrotic epithelial cells and epithelial ulceration. Therefore, APS could reduce the inflammatory cell infiltration in colonic tissues of UC model mice, repair the damaged colonic mucosa, and promote ulcer healing. In conclusion, APS has a potential application in the treatment of ulcerative colitis, and is dependent or partially dependent on MDSC to achieve this effects.},
 year = {2019}
}

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TY  - JOUR
T1  - Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis
AU  - Xi Qinhua
AU  - Teng Yajie
AU  - Li Yueqin
AU  - Dai Juan
AU  - Zhang Guangbo
AU  - Chen Weichang
Y1  - 2019/11/13
PY  - 2019
N1  - https://doi.org/10.11648/j.ajbls.20190706.13
DO  - 10.11648/j.ajbls.20190706.13
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 143
EP  - 147
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20190706.13
AB  - This study was performed to study the effects of astragalus polysaccharides (APS) in the treatment of ulcerative colitis (UC), and to explore whether myeloid-derived suppressor cells (MDSC) involve in this process. UC model was established by dextran sulfate sodium salt (DSS) inducement in mouse. Then the effects of APS on UC was evaluate at the levels of cytology and molecular biology: exploring the influence of APS on MDSC by analyzing the levels of MDSC before and after the treatments of APS; evaluating the therapeutic effects of APS on UC by pathologic histology. Data showed that the levels of MDSC in bone marrow, spleen, peripheral blood of UC mice were significantly decreased after intervention with APS, indicating APS inhibited the level of MDSC significantly. After depletion bone marrow cells in mice, we further found that the therapeutic effects of APS in MDSC- group was significantly reduced compared with MDSC+ group. It was discovered through pathological analysis that, compared with UC model group, APS intervention group mainly manifested as reduced infiltrating acute and chronic inflammatory cells, necrotic epithelial cells and epithelial ulceration. Therefore, APS could reduce the inflammatory cell infiltration in colonic tissues of UC model mice, repair the damaged colonic mucosa, and promote ulcer healing. In conclusion, APS has a potential application in the treatment of ulcerative colitis, and is dependent or partially dependent on MDSC to achieve this effects.
VL  - 7
IS  - 6
ER  -

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Author Information

Xi Qinhua

Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China
Teng Yajie

Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China
Li Yueqin

Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China
Dai Juan

Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China
Zhang Guangbo

Institute of Clinical Immunology, First Affiliated Hospital of Soochow University, Suzhou, China
Chen Weichang

Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China; Institute of Clinical Immunology, First Affiliated Hospital of Soochow University, Suzhou, China

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APA Style

Xi Qinhua, Teng Yajie, Li Yueqin, Dai Juan, Zhang Guangbo, et al. (2019). Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis. American Journal of Biomedical and Life Sciences, 7(6), 143-147. https://doi.org/10.11648/j.ajbls.20190706.13

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ACS Style

Xi Qinhua; Teng Yajie; Li Yueqin; Dai Juan; Zhang Guangbo, et al. Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis. Am. J. Biomed. Life Sci. 2019, 7(6), 143-147. doi: 10.11648/j.ajbls.20190706.13

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AMA Style

Xi Qinhua, Teng Yajie, Li Yueqin, Dai Juan, Zhang Guangbo, et al. Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis. Am J Biomed Life Sci. 2019;7(6):143-147. doi: 10.11648/j.ajbls.20190706.13

Copy | Download

@article{10.11648/j.ajbls.20190706.13,
  author = {Xi Qinhua and Teng Yajie and Li Yueqin and Dai Juan and Zhang Guangbo and Chen Weichang},
  title = {Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {7},
  number = {6},
  pages = {143-147},
  doi = {10.11648/j.ajbls.20190706.13},
  url = {https://doi.org/10.11648/j.ajbls.20190706.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20190706.13},
  abstract = {This study was performed to study the effects of astragalus polysaccharides (APS) in the treatment of ulcerative colitis (UC), and to explore whether myeloid-derived suppressor cells (MDSC) involve in this process. UC model was established by dextran sulfate sodium salt (DSS) inducement in mouse. Then the effects of APS on UC was evaluate at the levels of cytology and molecular biology: exploring the influence of APS on MDSC by analyzing the levels of MDSC before and after the treatments of APS; evaluating the therapeutic effects of APS on UC by pathologic histology. Data showed that the levels of MDSC in bone marrow, spleen, peripheral blood of UC mice were significantly decreased after intervention with APS, indicating APS inhibited the level of MDSC significantly. After depletion bone marrow cells in mice, we further found that the therapeutic effects of APS in MDSC- group was significantly reduced compared with MDSC+ group. It was discovered through pathological analysis that, compared with UC model group, APS intervention group mainly manifested as reduced infiltrating acute and chronic inflammatory cells, necrotic epithelial cells and epithelial ulceration. Therefore, APS could reduce the inflammatory cell infiltration in colonic tissues of UC model mice, repair the damaged colonic mucosa, and promote ulcer healing. In conclusion, APS has a potential application in the treatment of ulcerative colitis, and is dependent or partially dependent on MDSC to achieve this effects.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis
AU  - Xi Qinhua
AU  - Teng Yajie
AU  - Li Yueqin
AU  - Dai Juan
AU  - Zhang Guangbo
AU  - Chen Weichang
Y1  - 2019/11/13
PY  - 2019
N1  - https://doi.org/10.11648/j.ajbls.20190706.13
DO  - 10.11648/j.ajbls.20190706.13
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 143
EP  - 147
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20190706.13
AB  - This study was performed to study the effects of astragalus polysaccharides (APS) in the treatment of ulcerative colitis (UC), and to explore whether myeloid-derived suppressor cells (MDSC) involve in this process. UC model was established by dextran sulfate sodium salt (DSS) inducement in mouse. Then the effects of APS on UC was evaluate at the levels of cytology and molecular biology: exploring the influence of APS on MDSC by analyzing the levels of MDSC before and after the treatments of APS; evaluating the therapeutic effects of APS on UC by pathologic histology. Data showed that the levels of MDSC in bone marrow, spleen, peripheral blood of UC mice were significantly decreased after intervention with APS, indicating APS inhibited the level of MDSC significantly. After depletion bone marrow cells in mice, we further found that the therapeutic effects of APS in MDSC- group was significantly reduced compared with MDSC+ group. It was discovered through pathological analysis that, compared with UC model group, APS intervention group mainly manifested as reduced infiltrating acute and chronic inflammatory cells, necrotic epithelial cells and epithelial ulceration. Therefore, APS could reduce the inflammatory cell infiltration in colonic tissues of UC model mice, repair the damaged colonic mucosa, and promote ulcer healing. In conclusion, APS has a potential application in the treatment of ulcerative colitis, and is dependent or partially dependent on MDSC to achieve this effects.
VL  - 7
IS  - 6
ER  -

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