American Journal of Biomedical and Life Sciences

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Analysis of GNB Species and Pattern of Resistance Responsible for LRTI in Patients with Cancer

Received: 26 March 2015    Accepted: 09 April 2015    Published: 18 April 2015
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Abstract

Lower respiratory tract infection (LRTI) is the most lethal infection remains among patients undergoing treatment for cancer. Most of the previous studies with cancer patients have focus on blood stream infections. For that reason the aim of our study was to examine the spectrum and recent trends in antimicrobial resistance of Gram negative bacteria (GNB) recovered from cancer patient having LRTI in Egypt. In addition our objective was to investigate the prevalence and distribution of Legionella pneumophila among cancer patients with LRTI. Sputum specimens were collected from 285 cancer patients suspecting of having LRTI. The conventional methods and Microscan Negative Identification panel Type 2 were used for identification of GNB. Susceptibility was assessed for 20 antibiotics in bacterial isolates using agar diffusion method. All the sputum specimens were tested by culture and genus specific PCR for the detection of Legionella pneumophila. A total of 130 GNB were isolated. Among these, Klebsiella pneumoniae was the most common (35.4 %). We isolated and identified a number of less frequent GNB (17%), whereas no Legionella pneumophila was detected. Amikacin was found to be the most effective antimicrobial against GNB. We reported very high percentage of multi-drug resistance GNB (96%). This study reported the development of multidrug resistance Gram negative bacilli in Egypt. Continuous updating of data on antimicrobial susceptibility profiles is required to ensure the efficacy of antimicrobial agents against GNB due to continuous development of antimicrobial resistance patterns among these pathogens.

DOI 10.11648/j.ajbls.20150302.13
Published in American Journal of Biomedical and Life Sciences (Volume 3, Issue 2, April 2015)
Page(s) 25-32
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Lower Respiratory Tract Infection, Cancer Patients, Gram-Negative Bacteria, Legionella Pneumophila

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Author Information
  • Department of Microbiology and Immunology, Faculty of Pharmacy, Al-azhar University, Cairo, Egypt

  • Department of Microbiology and Immunology, Faculty of Pharmacy, Al-azhar University, Cairo, Egypt

  • Department of Clinical Pathology, National Cancer Institute, Cairo University, Cairo, Egypt

  • Department of Microbiology and Immunology, Faculty of Pharmacy, Al-azhar University, Cairo, Egypt

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    Salwa Selim Afifi, Zeinab Helal Helal, Safaa Shawky Hassan, Sally Tohamy Kamal. (2015). Analysis of GNB Species and Pattern of Resistance Responsible for LRTI in Patients with Cancer. American Journal of Biomedical and Life Sciences, 3(2), 25-32. https://doi.org/10.11648/j.ajbls.20150302.13

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    ACS Style

    Salwa Selim Afifi; Zeinab Helal Helal; Safaa Shawky Hassan; Sally Tohamy Kamal. Analysis of GNB Species and Pattern of Resistance Responsible for LRTI in Patients with Cancer. Am. J. Biomed. Life Sci. 2015, 3(2), 25-32. doi: 10.11648/j.ajbls.20150302.13

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    AMA Style

    Salwa Selim Afifi, Zeinab Helal Helal, Safaa Shawky Hassan, Sally Tohamy Kamal. Analysis of GNB Species and Pattern of Resistance Responsible for LRTI in Patients with Cancer. Am J Biomed Life Sci. 2015;3(2):25-32. doi: 10.11648/j.ajbls.20150302.13

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  • @article{10.11648/j.ajbls.20150302.13,
      author = {Salwa Selim Afifi and Zeinab Helal Helal and Safaa Shawky Hassan and Sally Tohamy Kamal},
      title = {Analysis of GNB Species and Pattern of Resistance Responsible for LRTI in Patients with Cancer},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {3},
      number = {2},
      pages = {25-32},
      doi = {10.11648/j.ajbls.20150302.13},
      url = {https://doi.org/10.11648/j.ajbls.20150302.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajbls.20150302.13},
      abstract = {Lower respiratory tract infection (LRTI) is the most lethal infection remains among patients undergoing treatment for cancer. Most of the previous studies with cancer patients have focus on blood stream infections. For that reason the aim of our study was to examine the spectrum and recent trends in antimicrobial resistance of Gram negative bacteria (GNB) recovered from cancer patient having LRTI in Egypt. In addition our objective was to investigate the prevalence and distribution of Legionella pneumophila among cancer patients with LRTI. Sputum specimens were collected from 285 cancer patients suspecting of having LRTI. The conventional methods and Microscan Negative Identification panel Type 2 were used for identification of GNB. Susceptibility was assessed for 20 antibiotics in bacterial isolates using agar diffusion method. All the sputum specimens were tested by culture and genus specific PCR for the detection of Legionella pneumophila. A total of 130 GNB were isolated. Among these, Klebsiella pneumoniae was the most common (35.4 %). We isolated and identified a number of less frequent GNB (17%), whereas no Legionella pneumophila was detected. Amikacin was found to be the most effective antimicrobial against GNB. We reported very high percentage of multi-drug resistance GNB (96%). This study reported the development of multidrug resistance Gram negative bacilli in Egypt. Continuous updating of data on antimicrobial susceptibility profiles is required to ensure the efficacy of antimicrobial agents against GNB due to continuous development of antimicrobial resistance patterns among these pathogens.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Analysis of GNB Species and Pattern of Resistance Responsible for LRTI in Patients with Cancer
    AU  - Salwa Selim Afifi
    AU  - Zeinab Helal Helal
    AU  - Safaa Shawky Hassan
    AU  - Sally Tohamy Kamal
    Y1  - 2015/04/18
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ajbls.20150302.13
    DO  - 10.11648/j.ajbls.20150302.13
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
    SP  - 25
    EP  - 32
    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20150302.13
    AB  - Lower respiratory tract infection (LRTI) is the most lethal infection remains among patients undergoing treatment for cancer. Most of the previous studies with cancer patients have focus on blood stream infections. For that reason the aim of our study was to examine the spectrum and recent trends in antimicrobial resistance of Gram negative bacteria (GNB) recovered from cancer patient having LRTI in Egypt. In addition our objective was to investigate the prevalence and distribution of Legionella pneumophila among cancer patients with LRTI. Sputum specimens were collected from 285 cancer patients suspecting of having LRTI. The conventional methods and Microscan Negative Identification panel Type 2 were used for identification of GNB. Susceptibility was assessed for 20 antibiotics in bacterial isolates using agar diffusion method. All the sputum specimens were tested by culture and genus specific PCR for the detection of Legionella pneumophila. A total of 130 GNB were isolated. Among these, Klebsiella pneumoniae was the most common (35.4 %). We isolated and identified a number of less frequent GNB (17%), whereas no Legionella pneumophila was detected. Amikacin was found to be the most effective antimicrobial against GNB. We reported very high percentage of multi-drug resistance GNB (96%). This study reported the development of multidrug resistance Gram negative bacilli in Egypt. Continuous updating of data on antimicrobial susceptibility profiles is required to ensure the efficacy of antimicrobial agents against GNB due to continuous development of antimicrobial resistance patterns among these pathogens.
    VL  - 3
    IS  - 2
    ER  - 

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