Evaluation of Dri-Dot OMPs Salmonella Typhi in Suspected Typhoid Fever Patients as an Immunodiagnostic Kit
American Journal of Biomedical and Life Sciences
Volume 3, Issue 4, August 2015, Pages: 87-90
Received: Jul. 17, 2015;
Accepted: Jul. 29, 2015;
Published: Aug. 13, 2015
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Yusriani Mangarengi, Department of Microbiology, Faculty of Medicine, Muslim University of Indonesia, Makassar, Indonesia; Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Ressy Dwiyanti, Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Nataniel Tandirogang, Department Microbiology and Immunology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia
Muhammad Sabir, Department of Microbiology, Faculty of Medicine, Tadulako University, Palu, Indonesia
Rosdiana Natzir, Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Mochammad Hatta, Molecular Biology and Immunology Laboratory for Infectious Diseases, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Yadi , Department Microbiology and Immunology, Faculty of Medicine, Mulawarman University, Samarinda, Indonesia
Background. Several studies have identified a protein of OMP S. Typhi. The protein is highly immunogenic and can be recognize whole cells of S. Typhi. The aims of the study was to evaluate the Dri-dot OMPs immunoreactivity in typhoid fever suspected patients. Method. Samples obtained from Dr. Wahidin Sudirohusodo Hospital, lbnu Sina Hospital, and Haji Hospital of Makassar from February to November 2014. Their sensitivity and specificity were evaluated against blood culture method as the gold standard. For each of the two tests, sensitivity, specificity, positive and negative predictive values were calculated using standard formulae. Results. A total of 15 of suspected typhoid fever samples examined for culture, lateral flow and Dri-dot OMPs. Examination of blood culture method showed that 3 of 15 (20%) patients had a blood culture positive for S. typhi. Dri-dot OMPs were positive in 13 (86.7%) serum samples. Fourteen (93.3%) serum samples were positive for lateral flow examination. Thirteen samples were positive for both Dri-dot and lateral flow. One sample was negative for both lateral flow and Dri-dot. One sample was positive for Dri-dot but negative for lateral flow. Conclusion. We conclude that there was no difference between Dri-dot OMPs, lateral flow and widal tests. Dri-dot could be of use for the diagnosis of typhoid fever in patients who have clinical typhoid fever
Evaluation of Dri-Dot OMPs Salmonella Typhi in Suspected Typhoid Fever Patients as an Immunodiagnostic Kit, American Journal of Biomedical and Life Sciences.
Vol. 3, No. 4,
2015, pp. 87-90.
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