Antioxidant Activity of Bioactive Protein of Kerang Kepah (<i>Atactodea striata</i>) from South Sulawesi

Tahirah Hasan; Abdul Wahid Wahab; Natsir Djide; Muhammad Zakir

doi:doi:10.11648/j.ajbls.20150306.12

| Peer-Reviewed

Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi

Tahirah Hasan, Abdul Wahid Wahab, Natsir Djide, Muhammad Zakir

Published in American Journal of Biomedical and Life Sciences (Volume 3, Issue 6)

Received: 8 October 2015 Accepted: 19 October 2015 Published: 13 November 2015

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Antioxidant activity test on protein extract of Kerang Kepah (Atactodea striata) by scavenging method of free radical of DPPH (N, N-diphenyl-N-picrylhydrazyl) has been carried out. This study was conducted to determine the antioxidant activity and the amino acid composition of the protein extract of Atactodea striata. The analysis results showed that the protein extract of Atactodea striata has the most complete amino acid composition and the highest antioxidant activity of the protein fraction at a rate of 30-50% ammonium sulfate saturation with IC50 value of 183.75µg/mL. However, when it is compared to the antioxidant activity of ascorbic acid (IC50 values = 2.51µg/mL), the antioxidant activity of the protein extract fraction of Atactodea striata is still much lower.

Published in	American Journal of Biomedical and Life Sciences (Volume 3, Issue 6)
DOI	10.11648/j.ajbls.20150306.12
Page(s)	111-114
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Atactodea striata, Antioxidant, DPPH, HPLC

References

[1]	J.L. Balcazar, R.Z. Blas, D. Cuningham, Vendrell and Muzquiz, The Rute of Probiotic in Aquaculture, Veterinary Microbiol. vol. 114, pp. 173-186, 2006.
[2]	J.W. Blunt, B.R. Copp, M.H.G. Munro, Northcote and M.R. Prinsep, Marine Natural Products. Natural Product Reports, vol. 23, pp. 26-78, 2006.
[3]	U. Grienke, J. Silke, D. Tasdemis, ”Bioactive compounds from marine mussels and their effects on human health,” Food Chem. Vol. 142, pp. 48-60, 2014.
[4]	R.D. Barnes, Invertebrate Zoology, Saunders College, Fourth Edition, 1982.
[5]	L. Nurjanah, D. Hardjito, Monintya, M. Bintang, and D.R. Agungpriyono,“Aktivitasantioksidanlintahlaut (Discodorissp) dariperairan Pulaulintahlaut dariperairan Pulau Buton Sulawesi Tenggara,” Prosiding Seminar Nasional Pengolahan Produkdan Bioteknologi Kelautandan Perikanan. Jakarta, 2009.
[6]	A. Nurjanah, Abdulla, and A. Apriand, “Aktivitas antioksidan dan komponen bioaktif pada keong ipong-ipong (Fasciolaria salmo),”J. Pengolahan Hasil Perikanan Indonesia, vol.14, pp 22-29, 2011.
[7]	C. Jiang, M. Wang, J. Lin, D. Gan, X. Zeng, “Extraction, preliminary characterization, antioxidant and anticancer activities in vitro of polysaccharides from Cyclinosinensis,” Carbohydrate Polymers, vol 84, pp. 851-857, 2011.
[8]	D.Y. Zhou, B.W. Zhu, L. Qiao, H.T. Wu, D.M. Li, J.F. Yang and Y. Murata, “In vitro antioxidant activity of enzymatic hydrolysates prepared from abalone (Haliotis discus hannaiIno) viscera,” Food and Bioproducts Processing, 2011.
[9]	V.N. Madhu, K. Sivaperumal, K. Kamala and B.G. Kulkarni, “Antibacterial and Antioxidant Activities of The Tissue Extract of Verna viridis From Versova Coast,” International Journal of Pharmacy and Pharmaceutical Sciences, Mumbai, 2014.
[10]	L. Huang, Protein dalam Air Mata Obat untuk AIDS, (On line), (http://www.rad .net.id/warta/wao4701.htm, (diakses 2 Januari 2013), 1999.
[11]	Nollet, M. L. Leo, “Handbook of Food Analysis and Amino Acid,” 1996.
[12]	D.T. Plummer, “An Introduction to Practical Biochemistry,” Second Edition. Tata Mc. Graw Hill Publishing Company Ltd. New Delhi, 1979.
[13]	R.K. Scopes, “Protein Purification Principles and Ptactice,” Second edition, Springger-Verlag, New York, 1987.
[14]	Liyana, F. Pathiranam, Shahidi,” Antioxidant activity of commercial soft and hard wheat (Triticum aestivum L.) as affected by gastric pH conditions,” J. Agric. Food Chem. Vol. 53, pp. 2430-2440. 2005.
[15]	W. Moka,“Identifikasi dan Inventarisasi Jenis Kerang Laut yang Digunakan Sebagai Obat Tradisonal di Sulawesi Selatan”, UNHAS, Makassar, 1982.

Cite This Article

Plain Text BibTeX RIS

APA Style

Tahirah Hasan, Abdul Wahid Wahab, Natsir Djide, Muhammad Zakir. (2015). Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi. American Journal of Biomedical and Life Sciences, 3(6), 111-114. https://doi.org/10.11648/j.ajbls.20150306.12

Copy | Download

ACS Style

Tahirah Hasan; Abdul Wahid Wahab; Natsir Djide; Muhammad Zakir. Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi. Am. J. Biomed. Life Sci. 2015, 3(6), 111-114. doi: 10.11648/j.ajbls.20150306.12

Copy | Download

AMA Style

Tahirah Hasan, Abdul Wahid Wahab, Natsir Djide, Muhammad Zakir. Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi. Am J Biomed Life Sci. 2015;3(6):111-114. doi: 10.11648/j.ajbls.20150306.12

Copy | Download

@article{10.11648/j.ajbls.20150306.12,
  author = {Tahirah Hasan and Abdul Wahid Wahab and Natsir Djide and Muhammad Zakir},
  title = {Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {3},
  number = {6},
  pages = {111-114},
  doi = {10.11648/j.ajbls.20150306.12},
  url = {https://doi.org/10.11648/j.ajbls.20150306.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20150306.12},
  abstract = {Antioxidant activity test on protein extract of Kerang Kepah (Atactodea striata) by scavenging method of free radical of DPPH (N, N-diphenyl-N-picrylhydrazyl) has been carried out. This study was conducted to determine the antioxidant activity and the amino acid composition of the protein extract of Atactodea striata. The analysis results showed that the protein extract of Atactodea striata has the most complete amino acid composition and the highest antioxidant activity of the protein fraction at a rate of 30-50% ammonium sulfate saturation with IC50 value of 183.75µg/mL. However, when it is compared to the antioxidant activity of ascorbic acid (IC50 values = 2.51µg/mL), the antioxidant activity of the protein extract fraction of Atactodea striata is still much lower.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi
AU  - Tahirah Hasan
AU  - Abdul Wahid Wahab
AU  - Natsir Djide
AU  - Muhammad Zakir
Y1  - 2015/11/13
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbls.20150306.12
DO  - 10.11648/j.ajbls.20150306.12
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 111
EP  - 114
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20150306.12
AB  - Antioxidant activity test on protein extract of Kerang Kepah (Atactodea striata) by scavenging method of free radical of DPPH (N, N-diphenyl-N-picrylhydrazyl) has been carried out. This study was conducted to determine the antioxidant activity and the amino acid composition of the protein extract of Atactodea striata. The analysis results showed that the protein extract of Atactodea striata has the most complete amino acid composition and the highest antioxidant activity of the protein fraction at a rate of 30-50% ammonium sulfate saturation with IC50 value of 183.75µg/mL. However, when it is compared to the antioxidant activity of ascorbic acid (IC50 values = 2.51µg/mL), the antioxidant activity of the protein extract fraction of Atactodea striata is still much lower.
VL  - 3
IS  - 6
ER  -

Copy | Download

Author Information

Tahirah Hasan

Department of Chemistry, Graduate School of Science, Hasanuddin University, Makassar 90245, South Sulawesi, Indonesia
Abdul Wahid Wahab

Department of Chemistry, Graduate School of Science, Hasanuddin University, Makassar 90245, South Sulawesi, Indonesia
Natsir Djide

Faculty of Pharmacy, Hasanuddin University, Makassar 90245, South Sulawesi, Indonesia
Muhammad Zakir

Department of Chemistry, Graduate School of Science, Hasanuddin University, Makassar 90245, South Sulawesi, Indonesia

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Tahirah Hasan, Abdul Wahid Wahab, Natsir Djide, Muhammad Zakir. (2015). Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi. American Journal of Biomedical and Life Sciences, 3(6), 111-114. https://doi.org/10.11648/j.ajbls.20150306.12

Copy | Download

ACS Style

Tahirah Hasan; Abdul Wahid Wahab; Natsir Djide; Muhammad Zakir. Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi. Am. J. Biomed. Life Sci. 2015, 3(6), 111-114. doi: 10.11648/j.ajbls.20150306.12

Copy | Download

AMA Style

Tahirah Hasan, Abdul Wahid Wahab, Natsir Djide, Muhammad Zakir. Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi. Am J Biomed Life Sci. 2015;3(6):111-114. doi: 10.11648/j.ajbls.20150306.12

Copy | Download

@article{10.11648/j.ajbls.20150306.12,
  author = {Tahirah Hasan and Abdul Wahid Wahab and Natsir Djide and Muhammad Zakir},
  title = {Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {3},
  number = {6},
  pages = {111-114},
  doi = {10.11648/j.ajbls.20150306.12},
  url = {https://doi.org/10.11648/j.ajbls.20150306.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20150306.12},
  abstract = {Antioxidant activity test on protein extract of Kerang Kepah (Atactodea striata) by scavenging method of free radical of DPPH (N, N-diphenyl-N-picrylhydrazyl) has been carried out. This study was conducted to determine the antioxidant activity and the amino acid composition of the protein extract of Atactodea striata. The analysis results showed that the protein extract of Atactodea striata has the most complete amino acid composition and the highest antioxidant activity of the protein fraction at a rate of 30-50% ammonium sulfate saturation with IC50 value of 183.75µg/mL. However, when it is compared to the antioxidant activity of ascorbic acid (IC50 values = 2.51µg/mL), the antioxidant activity of the protein extract fraction of Atactodea striata is still much lower.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi
AU  - Tahirah Hasan
AU  - Abdul Wahid Wahab
AU  - Natsir Djide
AU  - Muhammad Zakir
Y1  - 2015/11/13
PY  - 2015
N1  - https://doi.org/10.11648/j.ajbls.20150306.12
DO  - 10.11648/j.ajbls.20150306.12
T2  - American Journal of Biomedical and Life Sciences
JF  - American Journal of Biomedical and Life Sciences
JO  - American Journal of Biomedical and Life Sciences
SP  - 111
EP  - 114
PB  - Science Publishing Group
SN  - 2330-880X
UR  - https://doi.org/10.11648/j.ajbls.20150306.12
AB  - Antioxidant activity test on protein extract of Kerang Kepah (Atactodea striata) by scavenging method of free radical of DPPH (N, N-diphenyl-N-picrylhydrazyl) has been carried out. This study was conducted to determine the antioxidant activity and the amino acid composition of the protein extract of Atactodea striata. The analysis results showed that the protein extract of Atactodea striata has the most complete amino acid composition and the highest antioxidant activity of the protein fraction at a rate of 30-50% ammonium sulfate saturation with IC50 value of 183.75µg/mL. However, when it is compared to the antioxidant activity of ascorbic acid (IC50 values = 2.51µg/mL), the antioxidant activity of the protein extract fraction of Atactodea striata is still much lower.
VL  - 3
IS  - 6
ER  -

Copy | Download