American Journal of Biomedical and Life Sciences

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An Epidemiologic Study on Brucellosis in the Vicinity of Hohhot in China

Received: 09 August 2018    Accepted:     Published: 13 August 2018
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Abstract

In the past five years, the epidemic situation of brucellosis in Hohhot, Inner Mongolia, has been aggravated. The current diagnostic technology has low specificity. Through this topic, we can find a more convenient, safer, more specific and sensitive method for preparation. Better antigens are used for early rapid diagnosis of Brucellosis. Molecular biology techniques were used to design primers based on the published bp26 gene of Brucella from Gen-Bank. The DNA of Brucella isolates was extracted, PCR amplified and sequenced, and the bp26 gene sequence of the reference strain was identical to that of the reference strain. By comparing and analyzing the origins, the bp26 gene was amplified and compared with sheep, cattle, pigs, and sheep. In 2010, random surveys were conducted among 11401 people in 5331 farmers around Hohhot. The infection rate of Brucellosis was 3.82%, and 139059 of sheep were sampled in township and townships, and the positive rate was 2.82%. The results of bp26 gene sequence showed that the isolates of this experiment were 99.9% homologous to Brucella species 16M and Brucella species C68. The isolates were Brucella species 1320, Epididymis oryzae 280 and cattle. The homology of the Brucella strain S18 was 99.8%, 99.8%, and 98.9%, respectively. According to the phylogenetic tree analysis, the bp26 genes of Brucella strains of different strains are close, indicating that bp26 gene is very conservative. The homologous genes obtained by gene amplification technology can be used to detect br26 disease using bp26 protein. Other possible interferences can be ruled out and the diagnosis of Brucella can be promptly made. The culture and control of virulent strains of Brucella can be avoided. This experiment laid the foundation for the establishment of a new detection method using recombinant bp26 protein.

DOI 10.11648/j.ajbls.20180603.15
Published in American Journal of Biomedical and Life Sciences (Volume 6, Issue 3, June 2018)
Page(s) 63-68
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Brucellosis, Early Diagnosis, bp26 Gene, PCR

References
[1] Marzetti S, Carranza C, Roncallo M, et al. Recent trends in human Brucella canis infection. Comp Immunol Microbiol Infect Dis, 2012, 4 (12): 102-106.
[2] McGiven JA, Nicola A, Commander NJ, et al. An evaluation of the capability of existing and novel serodiagnostic methods for porcine brucellosis to reduce false positive serological reactions. Vet Microbiol, 2012.
[3] Ko KY, Kim JW, Her M, et al. Immunogenic proteins of Brucella abortus to minimize cross reactions in brucellosis diagnosis. Vet Microbiol, 2012, 156 (4): 374-380.
[4] Grilló MJ, Marín CM, Barberán M, et al. Efficacy of bp26 and bp26/omp31 B. melitensis Rev. 1 deletion mutants against Brucella ovis in rams. Vaccin, 2009, 27 (2): 187-191.
[5] Haag AF, Myka KK, Arnold MF, et al. Importance of Lipopolysaccharide and Cyclic β-1,2-Glucans in Brucella-Mammalian Infections. Int J Microbiol, 2010 Dec 1.
[6] Wang Y, Chen Z, Qiu Y, et al. Identification of Brucella abortus virulence proteins that modulate the host immune response. Bioengineered, 2012, 3 (5): 303-305.
[7] Seco-Mediavilla P, Verger JM, Grayon M, et al. Epitope mapping of the Brucella melitensis BP26 immunogenic protein: usefulness for diagnosis of sheep brucellosis. Clin Diagn Lab Immunol, 2003, 10 (4): 647-651.
[8] Cloeckaert A, Baucheron S, Vizcaino N, et al. Use of recombinant BP26 protein in serological diagnosis of Brucella melitensis infection in sheep. Clin Diagn Lab Immunol, 2001, 8 (4): 772-775.
[9] Qiu J, Wang W, Wu J, Characterization of periplasmic protein BP26 epitopes of Brucella melitensis reacting with murine monoclonal and sheep antibodies. PLoS One, 2012, 7 (3): 342-346.
[10] Kumar S, Tuteja U, Kumar A, et al. Expression and purification of the 26 kDa periplasmic protein of Brucella abortus: a reagent for the diagnosis of bovine brucellosis. Biotechnol Appl Biochem, 2008, 49 (3): 213-218.
[11] Tiwari AK, Kumar S, Pal V, et al. Evaluation of the recombinant 10-kilodalton immunodominant region of the BP26 protein of Brucella abortus for specific diagnosis of bovine brucellosis. Clin Vaccine Immunol, 2011, 18 (10): 1760-1764.
[12] Cloeckaert A, Debbarh HS, Vizcaíno N, et al. Cloning, nucleotide sequence, and expression of the Brucella melitensis bp26 gene coding for a protein immunogenic in infected sheep. FEMS Microbiol Lett, 1996, 140 (3): 139-144.
[13] Yang X, Hudson M, Walters N, et al. Selection of protective epitopes for Brucella melitensis by DNA vaccination. Infect Immun, 2005, 73 (11): 7297-7303.
[14] Roberts TW, Peck DE, Ritten JP. Cattle producers' economic incentives for preventing bovine brucellosis under uncertainty. Prev Vet Med, 2012, 107 (3): 187-203.
[15] Batsukh Z, Tsolmon B, Otgonbaatar D, et al. One Health in Mongolia. Curr Top Microbiol Immunol, 2012.
[16] Smits HL. Control and prevention of brucellosis in small ruminants: time for action. Vet Rec, 2012, 170 (4): 97-98.
[17] Iwashkiw JA, Fentabil MA, Faridmoayer A, et al. Exploiting the Campylobacter jejuni protein glycosylation system for glycoengineering vaccines and diagnostic tools directed against brucellosis. Microb Cell Fact, 2012, 25 (11): 13.
[18] Megersa B, Biffa D, Niguse F, Rufael T, et al. Cattle brucellosis in traditional livestock husbandry practice in Southern and Eastern Ethiopia, and its zoonotic implication. Acta Vet Scand, 2011, 7 (53): 24.
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Author Information
  • Department of Pharmacy, Affiliated Hospital of Inner Mongolian Medical University, Hohhot, P. R. China

  • Department of Pharmacy, Affiliated Hospital of Inner Mongolian Medical University, Hohhot, P. R. China

  • Department of Pharmacy, Affiliated Hospital of Inner Mongolian Medical University, Hohhot, P. R. China

  • Department of Pharmacy, Affiliated Hospital of Inner Mongolian Medical University, Hohhot, P. R. China

  • Department of Pharmacy, Affiliated Hospital of Inner Mongolian Medical University, Hohhot, P. R. China

Cite This Article
  • APA Style

    Xiaohui Wang, Dexi Li, Lidao Bao, Ruilian Ma, Sha Li. (2018). An Epidemiologic Study on Brucellosis in the Vicinity of Hohhot in China. American Journal of Biomedical and Life Sciences, 6(3), 63-68. https://doi.org/10.11648/j.ajbls.20180603.15

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    ACS Style

    Xiaohui Wang; Dexi Li; Lidao Bao; Ruilian Ma; Sha Li. An Epidemiologic Study on Brucellosis in the Vicinity of Hohhot in China. Am. J. Biomed. Life Sci. 2018, 6(3), 63-68. doi: 10.11648/j.ajbls.20180603.15

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    AMA Style

    Xiaohui Wang, Dexi Li, Lidao Bao, Ruilian Ma, Sha Li. An Epidemiologic Study on Brucellosis in the Vicinity of Hohhot in China. Am J Biomed Life Sci. 2018;6(3):63-68. doi: 10.11648/j.ajbls.20180603.15

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  • @article{10.11648/j.ajbls.20180603.15,
      author = {Xiaohui Wang and Dexi Li and Lidao Bao and Ruilian Ma and Sha Li},
      title = {An Epidemiologic Study on Brucellosis in the Vicinity of Hohhot in China},
      journal = {American Journal of Biomedical and Life Sciences},
      volume = {6},
      number = {3},
      pages = {63-68},
      doi = {10.11648/j.ajbls.20180603.15},
      url = {https://doi.org/10.11648/j.ajbls.20180603.15},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajbls.20180603.15},
      abstract = {In the past five years, the epidemic situation of brucellosis in Hohhot, Inner Mongolia, has been aggravated. The current diagnostic technology has low specificity. Through this topic, we can find a more convenient, safer, more specific and sensitive method for preparation. Better antigens are used for early rapid diagnosis of Brucellosis. Molecular biology techniques were used to design primers based on the published bp26 gene of Brucella from Gen-Bank. The DNA of Brucella isolates was extracted, PCR amplified and sequenced, and the bp26 gene sequence of the reference strain was identical to that of the reference strain. By comparing and analyzing the origins, the bp26 gene was amplified and compared with sheep, cattle, pigs, and sheep. In 2010, random surveys were conducted among 11401 people in 5331 farmers around Hohhot. The infection rate of Brucellosis was 3.82%, and 139059 of sheep were sampled in township and townships, and the positive rate was 2.82%. The results of bp26 gene sequence showed that the isolates of this experiment were 99.9% homologous to Brucella species 16M and Brucella species C68. The isolates were Brucella species 1320, Epididymis oryzae 280 and cattle. The homology of the Brucella strain S18 was 99.8%, 99.8%, and 98.9%, respectively. According to the phylogenetic tree analysis, the bp26 genes of Brucella strains of different strains are close, indicating that bp26 gene is very conservative. The homologous genes obtained by gene amplification technology can be used to detect br26 disease using bp26 protein. Other possible interferences can be ruled out and the diagnosis of Brucella can be promptly made. The culture and control of virulent strains of Brucella can be avoided. This experiment laid the foundation for the establishment of a new detection method using recombinant bp26 protein.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - An Epidemiologic Study on Brucellosis in the Vicinity of Hohhot in China
    AU  - Xiaohui Wang
    AU  - Dexi Li
    AU  - Lidao Bao
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    DO  - 10.11648/j.ajbls.20180603.15
    T2  - American Journal of Biomedical and Life Sciences
    JF  - American Journal of Biomedical and Life Sciences
    JO  - American Journal of Biomedical and Life Sciences
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    PB  - Science Publishing Group
    SN  - 2330-880X
    UR  - https://doi.org/10.11648/j.ajbls.20180603.15
    AB  - In the past five years, the epidemic situation of brucellosis in Hohhot, Inner Mongolia, has been aggravated. The current diagnostic technology has low specificity. Through this topic, we can find a more convenient, safer, more specific and sensitive method for preparation. Better antigens are used for early rapid diagnosis of Brucellosis. Molecular biology techniques were used to design primers based on the published bp26 gene of Brucella from Gen-Bank. The DNA of Brucella isolates was extracted, PCR amplified and sequenced, and the bp26 gene sequence of the reference strain was identical to that of the reference strain. By comparing and analyzing the origins, the bp26 gene was amplified and compared with sheep, cattle, pigs, and sheep. In 2010, random surveys were conducted among 11401 people in 5331 farmers around Hohhot. The infection rate of Brucellosis was 3.82%, and 139059 of sheep were sampled in township and townships, and the positive rate was 2.82%. The results of bp26 gene sequence showed that the isolates of this experiment were 99.9% homologous to Brucella species 16M and Brucella species C68. The isolates were Brucella species 1320, Epididymis oryzae 280 and cattle. The homology of the Brucella strain S18 was 99.8%, 99.8%, and 98.9%, respectively. According to the phylogenetic tree analysis, the bp26 genes of Brucella strains of different strains are close, indicating that bp26 gene is very conservative. The homologous genes obtained by gene amplification technology can be used to detect br26 disease using bp26 protein. Other possible interferences can be ruled out and the diagnosis of Brucella can be promptly made. The culture and control of virulent strains of Brucella can be avoided. This experiment laid the foundation for the establishment of a new detection method using recombinant bp26 protein.
    VL  - 6
    IS  - 3
    ER  - 

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