American Journal of Health Research

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Effect of Biofield Energy Healing Treatment (The Trivedi Effect®) Based Herbomineral Formulation on Pro-Inflammatory Cytokines Expression in Murine Dendritic and Splenocyte Cells

Received: 15 November 2016    Accepted: 26 November 2016    Published: 08 December 2016
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Abstract

The utilization and demand of self-medication with herbomineral-based formulations have increased day-by-day across the globe over the last decade. A new proprietary herbomineral formulation was prepared with the mixture of minerals (zinc, magnesium, and selenium) and the herbal root extract of ashwagandha. The current study was undertaken to evaluate the Biofield Energy Healing (The Trivedi Effect®) on the test herbomineral formulation using murine dendritic cells (DCs) and splenocytes in vitro. The formulation was divided into two parts, one part was control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The effect of the Biofield Energy Treated formulation in murine cells was monitored with an estimation of pro-inflammatory cytokines levels such as tumor necrosis factor (TNF-α), macrophage inflammatory protein-1α (MIP-1α) and interleukin (IL-1β) in cell culture supernatants along with estimations of non-cytotoxic concentrations of the test formulation by MTT assay. The Biofield Treated formulation showed 114.2%, 122.6%, 141.2%, 127.8%, and 114.1% cell viability at concentrations 1.05, 5.2, 10.5, 25.6, and 51.2 µg/mL, respectively in DCs. Similarly, the Biofield Energy Treated and untreated formulations showed more than 100% cell viability in mice splenocytes at 5 µg/mL. The level of TNF-α in DCs was significantly (p≤0.05) inhibited by 19.21% in the Biofield Treated formulation at concentration 5.2 µg/mL as compared to the untreated test formulation. The level of MIP-1α in LPS induced mice splenocyte cells was reduced by 15.35% in the Biofield Energy Treated formulation at 0.0105 µg/mL as compared to the untreated formulation. Similarly, the level of IL-1β in LPS induced mice splenocyte cells was significantly (p≤0.05) reduced by 31.59% in the Biofield Treated formulation at 1.05 µg/mL as compared to the untreated formulation. Altogether, the results suggest that The Trivedi Effect® (Biofield Energy Healing Treatment) showed significant down-regulation of the tested pro-inflammatory cytokines expression and potentiated the immunosuppressive effect of the treated formulation to modulate the immune system. These data also suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.

DOI 10.11648/j.ajhr.20160406.15
Published in American Journal of Health Research (Volume 4, Issue 6, November 2016)
Page(s) 179-188
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Biofield Energy Healing Treatment, Biofield Energy Healers, The Trivedi Effect®, Immunomodulation, Dendritic Cells, Splenocytes, Cytokines

References
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[24] Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S Evaluation of physical and structural properties of biofield energy treated barium calcium tungsten oxide. Advances in Materials 4: 95-100.
[25] Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O, Jana S (2015) Impact of biofield treatment on atomic and structural characteristics of barium titanate powder. Ind Eng Manage 4: 166.
[26] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Impact of biofield energy treatment on soil fertility. Earth sciences 4: 275-279.
[27] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of biochemical marker - glutathione and DNA fingerprinting of biofield energy treated Oryza sativa. American Journal of BioScience 3: 243-248.
[28] Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of plant growth regulator, immunity and DNA fingerprinting of biofield energy treated mustard seeds (Brassica juncea). Agriculture Forestry and Fisheries 4: 269-274.
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Author Information
  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Global, Inc., Henderson, Nevada, USA

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

  • Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

Cite This Article
  • APA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, et al. (2016). Effect of Biofield Energy Healing Treatment (The Trivedi Effect®) Based Herbomineral Formulation on Pro-Inflammatory Cytokines Expression in Murine Dendritic and Splenocyte Cells. American Journal of Health Research, 4(6), 179-188. https://doi.org/10.11648/j.ajhr.20160406.15

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    ACS Style

    Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Cathryn Dawn Nykvist, et al. Effect of Biofield Energy Healing Treatment (The Trivedi Effect®) Based Herbomineral Formulation on Pro-Inflammatory Cytokines Expression in Murine Dendritic and Splenocyte Cells. Am. J. Health Res. 2016, 4(6), 179-188. doi: 10.11648/j.ajhr.20160406.15

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    AMA Style

    Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, et al. Effect of Biofield Energy Healing Treatment (The Trivedi Effect®) Based Herbomineral Formulation on Pro-Inflammatory Cytokines Expression in Murine Dendritic and Splenocyte Cells. Am J Health Res. 2016;4(6):179-188. doi: 10.11648/j.ajhr.20160406.15

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  • @article{10.11648/j.ajhr.20160406.15,
      author = {Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Cathryn Dawn Nykvist and Celine Lavelle and Daniel Paul Przybylski and Dianne Heather Vincent and Dorothy Felger and Douglas Jay Konersman and Elizabeth Ann Feeney and Jay Anthony Prague and Joanne Lydia Starodub and Karan Rasdan and Karen Mie Strassman and Leonid Soboleff and Maire Anne Mayne and Mary M. Keesee and Padmanabha Narayana Pillai and Pamela Clarkson Ansley and Ronald David Schmitz and Sharyn Marie Sodomora and Sambhu Charan Mondal and Snehasis Jana},
      title = {Effect of Biofield Energy Healing Treatment (The Trivedi Effect®) Based Herbomineral Formulation on Pro-Inflammatory Cytokines Expression in Murine Dendritic and Splenocyte Cells},
      journal = {American Journal of Health Research},
      volume = {4},
      number = {6},
      pages = {179-188},
      doi = {10.11648/j.ajhr.20160406.15},
      url = {https://doi.org/10.11648/j.ajhr.20160406.15},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajhr.20160406.15},
      abstract = {The utilization and demand of self-medication with herbomineral-based formulations have increased day-by-day across the globe over the last decade. A new proprietary herbomineral formulation was prepared with the mixture of minerals (zinc, magnesium, and selenium) and the herbal root extract of ashwagandha. The current study was undertaken to evaluate the Biofield Energy Healing (The Trivedi Effect®) on the test herbomineral formulation using murine dendritic cells (DCs) and splenocytes in vitro. The formulation was divided into two parts, one part was control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The effect of the Biofield Energy Treated formulation in murine cells was monitored with an estimation of pro-inflammatory cytokines levels such as tumor necrosis factor (TNF-α), macrophage inflammatory protein-1α (MIP-1α) and interleukin (IL-1β) in cell culture supernatants along with estimations of non-cytotoxic concentrations of the test formulation by MTT assay. The Biofield Treated formulation showed 114.2%, 122.6%, 141.2%, 127.8%, and 114.1% cell viability at concentrations 1.05, 5.2, 10.5, 25.6, and 51.2 µg/mL, respectively in DCs. Similarly, the Biofield Energy Treated and untreated formulations showed more than 100% cell viability in mice splenocytes at 5 µg/mL. The level of TNF-α in DCs was significantly (p≤0.05) inhibited by 19.21% in the Biofield Treated formulation at concentration 5.2 µg/mL as compared to the untreated test formulation. The level of MIP-1α in LPS induced mice splenocyte cells was reduced by 15.35% in the Biofield Energy Treated formulation at 0.0105 µg/mL as compared to the untreated formulation. Similarly, the level of IL-1β in LPS induced mice splenocyte cells was significantly (p≤0.05) reduced by 31.59% in the Biofield Treated formulation at 1.05 µg/mL as compared to the untreated formulation. Altogether, the results suggest that The Trivedi Effect® (Biofield Energy Healing Treatment) showed significant down-regulation of the tested pro-inflammatory cytokines expression and potentiated the immunosuppressive effect of the treated formulation to modulate the immune system. These data also suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Effect of Biofield Energy Healing Treatment (The Trivedi Effect®) Based Herbomineral Formulation on Pro-Inflammatory Cytokines Expression in Murine Dendritic and Splenocyte Cells
    AU  - Mahendra Kumar Trivedi
    AU  - Alice Branton
    AU  - Dahryn Trivedi
    AU  - Gopal Nayak
    AU  - Cathryn Dawn Nykvist
    AU  - Celine Lavelle
    AU  - Daniel Paul Przybylski
    AU  - Dianne Heather Vincent
    AU  - Dorothy Felger
    AU  - Douglas Jay Konersman
    AU  - Elizabeth Ann Feeney
    AU  - Jay Anthony Prague
    AU  - Joanne Lydia Starodub
    AU  - Karan Rasdan
    AU  - Karen Mie Strassman
    AU  - Leonid Soboleff
    AU  - Maire Anne Mayne
    AU  - Mary M. Keesee
    AU  - Padmanabha Narayana Pillai
    AU  - Pamela Clarkson Ansley
    AU  - Ronald David Schmitz
    AU  - Sharyn Marie Sodomora
    AU  - Sambhu Charan Mondal
    AU  - Snehasis Jana
    Y1  - 2016/12/08
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ajhr.20160406.15
    DO  - 10.11648/j.ajhr.20160406.15
    T2  - American Journal of Health Research
    JF  - American Journal of Health Research
    JO  - American Journal of Health Research
    SP  - 179
    EP  - 188
    PB  - Science Publishing Group
    SN  - 2330-8796
    UR  - https://doi.org/10.11648/j.ajhr.20160406.15
    AB  - The utilization and demand of self-medication with herbomineral-based formulations have increased day-by-day across the globe over the last decade. A new proprietary herbomineral formulation was prepared with the mixture of minerals (zinc, magnesium, and selenium) and the herbal root extract of ashwagandha. The current study was undertaken to evaluate the Biofield Energy Healing (The Trivedi Effect®) on the test herbomineral formulation using murine dendritic cells (DCs) and splenocytes in vitro. The formulation was divided into two parts, one part was control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The effect of the Biofield Energy Treated formulation in murine cells was monitored with an estimation of pro-inflammatory cytokines levels such as tumor necrosis factor (TNF-α), macrophage inflammatory protein-1α (MIP-1α) and interleukin (IL-1β) in cell culture supernatants along with estimations of non-cytotoxic concentrations of the test formulation by MTT assay. The Biofield Treated formulation showed 114.2%, 122.6%, 141.2%, 127.8%, and 114.1% cell viability at concentrations 1.05, 5.2, 10.5, 25.6, and 51.2 µg/mL, respectively in DCs. Similarly, the Biofield Energy Treated and untreated formulations showed more than 100% cell viability in mice splenocytes at 5 µg/mL. The level of TNF-α in DCs was significantly (p≤0.05) inhibited by 19.21% in the Biofield Treated formulation at concentration 5.2 µg/mL as compared to the untreated test formulation. The level of MIP-1α in LPS induced mice splenocyte cells was reduced by 15.35% in the Biofield Energy Treated formulation at 0.0105 µg/mL as compared to the untreated formulation. Similarly, the level of IL-1β in LPS induced mice splenocyte cells was significantly (p≤0.05) reduced by 31.59% in the Biofield Treated formulation at 1.05 µg/mL as compared to the untreated formulation. Altogether, the results suggest that The Trivedi Effect® (Biofield Energy Healing Treatment) showed significant down-regulation of the tested pro-inflammatory cytokines expression and potentiated the immunosuppressive effect of the treated formulation to modulate the immune system. These data also suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.
    VL  - 4
    IS  - 6
    ER  - 

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