Isolation and Antimicrobial Sensitivity Patterns of Enteric Bacterial Pathogens from Asymptomatic Food Handlers, Jimma, Ethiopia
American Journal of Health Research
Volume 3, Issue 6, November 2015, Pages: 399-406
Received: Oct. 12, 2015; Accepted: Oct. 26, 2015; Published: Jan. 4, 2016
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Authors
Teshale Worku, Department of Biomedical Science, College of Health Sciences, Mizan Tepi University, MizanTeferi, Ethiopia
Ayalew Jejaw, Department of Biomedical Science, College of Health Sciences, Mizan Tepi University, MizanTeferi, Ethiopia
Subbaram Kannan, Departmentof Medical Laboratory Sciences, College of Health and Medical Sciences, Jimma University, Jimma, Ethiopia
Beyene Wondafrash, Departmentof Medical Laboratory Sciences, College of Health and Medical Sciences, Jimma University, Jimma, Ethiopia
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Abstract
Background: Most enteric bacteria are harmless but species which belong to Salmonella, Shigella, Yersinia and certain strains of Escherichia coli are pathogens. The emergences of increased antimicrobial resistances are global challenges, particularly in developing countries due to misuse of antimicrobial agents. This study was aimed at determining the prevalence and antimicrobial sensitivity patterns of enteric bacterial pathogens isolated from food handlers in Jimma Town, Ethiopia. Methods: A cross-sectional study was conducted from July 16/2012 to November 23/2012 among 218 food handlers. Structured questionnaire was used to assess associated factors for enteric bacterial infection. Stool samples were collected andantimicrobial sensitivity tests were done using Mueller Hinton Agar (MHA) by Kirby-Bauer disc diffusion method following standard procedures. Descriptive statistics were performed using SPSS version 16.P-values less than 0.05 were considered as statistically significant. Results: The prevalence of enteric bacterial pathogens was found to be 6.9% (15/218). All S. typhi isolates showed resistance to two antimicrobials; amoxicillin-clavulanic acid and streptomycin. However, they all showed sensitivity to most antimicrobials. Half of Shigella isolates showed resistance to two antimicrobials; chloramphenicol and trimethoprim-sulfamethoxazole where as the other half showed resistant toother four antimicrobials; amoxicillin-clavulanic acid, ampicillin, streptomycin and tetracycline. One-third of enterohaemorrhagic Escherichia coli (EHEC) O157:H7 strains of the isolates were found to be resistant to two antimicrobials; amoxicillin-clavulanic acid and tetracycline. Enteric bacterial infection had a significant association with poor training in food handling and preparation (p=0.034), not knowledgeable about food borne infection (p<0.001) and inconsistent hand washing (p=0.033). Conclusion: Most of the isolates were resistant to ampicillin, streptomycin and tetracycline while all were sensitive to ciprofloxacin and nalidixic acid. Health education along with continuous food safety training should be given to food handlers so that they can adhere with effective hygienic practices.
Keywords
Antimicrobial, Enteric Bacterial Pathogens, Food Handler, Sensitivity
To cite this article
Teshale Worku, Ayalew Jejaw, Subbaram Kannan, Beyene Wondafrash, Isolation and Antimicrobial Sensitivity Patterns of Enteric Bacterial Pathogens from Asymptomatic Food Handlers, Jimma, Ethiopia, American Journal of Health Research. Vol. 3, No. 6, 2015, pp. 399-406. doi: 10.11648/j.ajhr.20150306.24
Copyright
Copyright © 2015 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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