Effect of Biofield Energy Healing Treatment (The Trivedi Effect®) Based Herbomineral Formulation on Pro-Inflammatory Cytokines Expression in Murine Dendritic and Splenocyte Cells
American Journal of Health Research
Volume 4, Issue 6, November 2016, Pages: 179-188
Received: Nov. 15, 2016; Accepted: Nov. 26, 2016; Published: Dec. 8, 2016
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Authors
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton, Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, Nevada, USA
Cathryn Dawn Nykvist, Trivedi Global, Inc., Henderson, Nevada, USA
Celine Lavelle, Trivedi Global, Inc., Henderson, Nevada, USA
Daniel Paul Przybylski, Trivedi Global, Inc., Henderson, Nevada, USA
Dianne Heather Vincent, Trivedi Global, Inc., Henderson, Nevada, USA
Dorothy Felger, Trivedi Global, Inc., Henderson, Nevada, USA
Douglas Jay Konersman, Trivedi Global, Inc., Henderson, Nevada, USA
Elizabeth Ann Feeney, Trivedi Global, Inc., Henderson, Nevada, USA
Jay Anthony Prague, Trivedi Global, Inc., Henderson, Nevada, USA
Joanne Lydia Starodub, Trivedi Global, Inc., Henderson, Nevada, USA
Karan Rasdan, Trivedi Global, Inc., Henderson, Nevada, USA
Karen Mie Strassman, Trivedi Global, Inc., Henderson, Nevada, USA
Leonid Soboleff, Trivedi Global, Inc., Henderson, Nevada, USA
Maire Anne Mayne, Trivedi Global, Inc., Henderson, Nevada, USA
Mary M. Keesee, Trivedi Global, Inc., Henderson, Nevada, USA
Padmanabha Narayana Pillai, Trivedi Global, Inc., Henderson, Nevada, USA
Pamela Clarkson Ansley, Trivedi Global, Inc., Henderson, Nevada, USA
Ronald David Schmitz, Trivedi Global, Inc., Henderson, Nevada, USA
Sharyn Marie Sodomora, Trivedi Global, Inc., Henderson, Nevada, USA
Sambhu Charan Mondal, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
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Abstract
The utilization and demand of self-medication with herbomineral-based formulations have increased day-by-day across the globe over the last decade. A new proprietary herbomineral formulation was prepared with the mixture of minerals (zinc, magnesium, and selenium) and the herbal root extract of ashwagandha. The current study was undertaken to evaluate the Biofield Energy Healing (The Trivedi Effect®) on the test herbomineral formulation using murine dendritic cells (DCs) and splenocytes in vitro. The formulation was divided into two parts, one part was control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The effect of the Biofield Energy Treated formulation in murine cells was monitored with an estimation of pro-inflammatory cytokines levels such as tumor necrosis factor (TNF-α), macrophage inflammatory protein-1α (MIP-1α) and interleukin (IL-1β) in cell culture supernatants along with estimations of non-cytotoxic concentrations of the test formulation by MTT assay. The Biofield Treated formulation showed 114.2%, 122.6%, 141.2%, 127.8%, and 114.1% cell viability at concentrations 1.05, 5.2, 10.5, 25.6, and 51.2 µg/mL, respectively in DCs. Similarly, the Biofield Energy Treated and untreated formulations showed more than 100% cell viability in mice splenocytes at 5 µg/mL. The level of TNF-α in DCs was significantly (p≤0.05) inhibited by 19.21% in the Biofield Treated formulation at concentration 5.2 µg/mL as compared to the untreated test formulation. The level of MIP-1α in LPS induced mice splenocyte cells was reduced by 15.35% in the Biofield Energy Treated formulation at 0.0105 µg/mL as compared to the untreated formulation. Similarly, the level of IL-1β in LPS induced mice splenocyte cells was significantly (p≤0.05) reduced by 31.59% in the Biofield Treated formulation at 1.05 µg/mL as compared to the untreated formulation. Altogether, the results suggest that The Trivedi Effect® (Biofield Energy Healing Treatment) showed significant down-regulation of the tested pro-inflammatory cytokines expression and potentiated the immunosuppressive effect of the treated formulation to modulate the immune system. These data also suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.
Keywords
Biofield Energy Healing Treatment, Biofield Energy Healers, The Trivedi Effect®, Immunomodulation, Dendritic Cells, Splenocytes, Cytokines
To cite this article
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Cathryn Dawn Nykvist, Celine Lavelle, Daniel Paul Przybylski, Dianne Heather Vincent, Dorothy Felger, Douglas Jay Konersman, Elizabeth Ann Feeney, Jay Anthony Prague, Joanne Lydia Starodub, Karan Rasdan, Karen Mie Strassman, Leonid Soboleff, Maire Anne Mayne, Mary M. Keesee, Padmanabha Narayana Pillai, Pamela Clarkson Ansley, Ronald David Schmitz, Sharyn Marie Sodomora, Sambhu Charan Mondal, Snehasis Jana, Effect of Biofield Energy Healing Treatment (The Trivedi Effect®) Based Herbomineral Formulation on Pro-Inflammatory Cytokines Expression in Murine Dendritic and Splenocyte Cells, American Journal of Health Research. Vol. 4, No. 6, 2016, pp. 179-188. doi: 10.11648/j.ajhr.20160406.15
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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