Spectrum of Uropathogens and Antimicrobial Susceptibility in Clinically Diagnosed Cases of Urinary Tract Infection in the Bamenda Regional Hospital, Cameroon

Marie Ebob Agbortabot Bissong; Carlson Mbah; Frank Eric Tatsing Foka; Henri-Lucien Kamga

doi:doi:10.11648/j.ajhr.20170502.11

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Spectrum of Uropathogens and Antimicrobial Susceptibility in Clinically Diagnosed Cases of Urinary Tract Infection in the Bamenda Regional Hospital, Cameroon

Marie Ebob Agbortabot Bissong, Carlson Mbah, Frank Eric Tatsing Foka, Henri-Lucien Kamga

Published in American Journal of Health Research (Volume 5, Issue 2)

Received: 8 December 2016 Accepted: 9 January 2017 Published: 2 March 2017

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Abstract

Urinary tract infections (UTIs) are among the common bacterial infections in humans both in the community and hospital settings. In spite of the availability and use of antimicrobial drugs, UTIs have shown increasing trends in recent years. Antimicrobial resistance among uropathogens is a serious health threat as this may complicate treatment, eventually resulting to more severe disease. Despite the importance of UTI, there is paucity of information on the aetiology of this disease especially in the Northwest Region. For that reason, the present study was designed with the aim to determine the spectrum of uropathogens and antimicrobial resistance of isolates in cases of symptomatic bacteriuria. To achieve this goal, midstream urine specimens were inoculated unto CLED media and isolates were identified using the Enterosystem 18R. Significant bacteriuria was considered with bacterial growth of ≥10⁵ CFU/ml. Susceptibility testing was performed using the Kirby Bauer disk diffusion method and the accuracy of the dipstick test was determined using culture as a gold standard. Significant bacteriuria was observed in 50% of the participants and the predominant uropathogens isolated were P. aeruginosa (22.4%), Klebsiella spp (14.3%), S. aureus (14.3%), CNS (14.3%) and E. coli (12.2%). High resistance (80-98%) among uropathogens was observed against clindamycin, amoxicillin and doxycycline. For the dipstick test, leucocyte esterase and nitrite showed high sensitivity (93.9%) and specificity (100%) respectively, for detecting UTI. The results of this study highlights the importance of Pseudomonas aeruginosa in the aetiology of both community and hospital acquired UTIs. In addition, gentamicin is the most effective agent against uropathogens in the study area.

Published in	American Journal of Health Research (Volume 5, Issue 2)
DOI	10.11648/j.ajhr.20170502.11
Page(s)	19-24
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

UTI, Symptomatic Bacteriuria, Aetiology, Screening Methods, Antimicrobial Resistance, Cameroon

References

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Marie Ebob Agbortabot Bissong, Carlson Mbah, Frank Eric Tatsing Foka, Henri-Lucien Kamga. (2017). Spectrum of Uropathogens and Antimicrobial Susceptibility in Clinically Diagnosed Cases of Urinary Tract Infection in the Bamenda Regional Hospital, Cameroon. American Journal of Health Research, 5(2), 19-24. https://doi.org/10.11648/j.ajhr.20170502.11

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ACS Style

Marie Ebob Agbortabot Bissong; Carlson Mbah; Frank Eric Tatsing Foka; Henri-Lucien Kamga. Spectrum of Uropathogens and Antimicrobial Susceptibility in Clinically Diagnosed Cases of Urinary Tract Infection in the Bamenda Regional Hospital, Cameroon. Am. J. Health Res. 2017, 5(2), 19-24. doi: 10.11648/j.ajhr.20170502.11

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AMA Style

Marie Ebob Agbortabot Bissong, Carlson Mbah, Frank Eric Tatsing Foka, Henri-Lucien Kamga. Spectrum of Uropathogens and Antimicrobial Susceptibility in Clinically Diagnosed Cases of Urinary Tract Infection in the Bamenda Regional Hospital, Cameroon. Am J Health Res. 2017;5(2):19-24. doi: 10.11648/j.ajhr.20170502.11

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@article{10.11648/j.ajhr.20170502.11,
  author = {Marie Ebob Agbortabot Bissong and Carlson Mbah and Frank Eric Tatsing Foka and Henri-Lucien Kamga},
  title = {Spectrum of Uropathogens and Antimicrobial Susceptibility in Clinically Diagnosed Cases of Urinary Tract Infection in the Bamenda Regional Hospital, Cameroon},
  journal = {American Journal of Health Research},
  volume = {5},
  number = {2},
  pages = {19-24},
  doi = {10.11648/j.ajhr.20170502.11},
  url = {https://doi.org/10.11648/j.ajhr.20170502.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20170502.11},
  abstract = {Urinary tract infections (UTIs) are among the common bacterial infections in humans both in the community and hospital settings. In spite of the availability and use of antimicrobial drugs, UTIs have shown increasing trends in recent years. Antimicrobial resistance among uropathogens is a serious health threat as this may complicate treatment, eventually resulting to more severe disease. Despite the importance of UTI, there is paucity of information on the aetiology of this disease especially in the Northwest Region. For that reason, the present study was designed with the aim to determine the spectrum of uropathogens and antimicrobial resistance of isolates in cases of symptomatic bacteriuria. To achieve this goal, midstream urine specimens were inoculated unto CLED media and isolates were identified using the Enterosystem 18R. Significant bacteriuria was considered with bacterial growth of ≥105 CFU/ml. Susceptibility testing was performed using the Kirby Bauer disk diffusion method and the accuracy of the dipstick test was determined using culture as a gold standard. Significant bacteriuria was observed in 50% of the participants and the predominant uropathogens isolated were P. aeruginosa (22.4%), Klebsiella spp (14.3%), S. aureus (14.3%), CNS (14.3%) and E. coli (12.2%). High resistance (80-98%) among uropathogens was observed against clindamycin, amoxicillin and doxycycline. For the dipstick test, leucocyte esterase and nitrite showed high sensitivity (93.9%) and specificity (100%) respectively, for detecting UTI. The results of this study highlights the importance of Pseudomonas aeruginosa in the aetiology of both community and hospital acquired UTIs. In addition, gentamicin is the most effective agent against uropathogens in the study area.},
 year = {2017}
}

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TY - JOUR
T1 - Spectrum of Uropathogens and Antimicrobial Susceptibility in Clinically Diagnosed Cases of Urinary Tract Infection in the Bamenda Regional Hospital, Cameroon
AU - Marie Ebob Agbortabot Bissong
AU - Carlson Mbah
AU - Frank Eric Tatsing Foka
AU - Henri-Lucien Kamga
Y1 - 2017/03/02
PY - 2017
N1 - https://doi.org/10.11648/j.ajhr.20170502.11
DO - 10.11648/j.ajhr.20170502.11
T2 - American Journal of Health Research
JF - American Journal of Health Research
JO - American Journal of Health Research
SP - 19
EP - 24
PB - Science Publishing Group
SN - 2330-8796
UR - https://doi.org/10.11648/j.ajhr.20170502.11
AB - Urinary tract infections (UTIs) are among the common bacterial infections in humans both in the community and hospital settings. In spite of the availability and use of antimicrobial drugs, UTIs have shown increasing trends in recent years. Antimicrobial resistance among uropathogens is a serious health threat as this may complicate treatment, eventually resulting to more severe disease. Despite the importance of UTI, there is paucity of information on the aetiology of this disease especially in the Northwest Region. For that reason, the present study was designed with the aim to determine the spectrum of uropathogens and antimicrobial resistance of isolates in cases of symptomatic bacteriuria. To achieve this goal, midstream urine specimens were inoculated unto CLED media and isolates were identified using the Enterosystem 18R. Significant bacteriuria was considered with bacterial growth of ≥105 CFU/ml. Susceptibility testing was performed using the Kirby Bauer disk diffusion method and the accuracy of the dipstick test was determined using culture as a gold standard. Significant bacteriuria was observed in 50% of the participants and the predominant uropathogens isolated were P. aeruginosa (22.4%), Klebsiella spp (14.3%), S. aureus (14.3%), CNS (14.3%) and E. coli (12.2%). High resistance (80-98%) among uropathogens was observed against clindamycin, amoxicillin and doxycycline. For the dipstick test, leucocyte esterase and nitrite showed high sensitivity (93.9%) and specificity (100%) respectively, for detecting UTI. The results of this study highlights the importance of Pseudomonas aeruginosa in the aetiology of both community and hospital acquired UTIs. In addition, gentamicin is the most effective agent against uropathogens in the study area.
VL - 5
IS - 2
ER -

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Author Information

Marie Ebob Agbortabot Bissong

Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Bamenda, Bamenda, Cameroon; Department of Biological Sciences, Faculty of Agriculture, Science and Technology, North West University, Mafikeng, South Africa
Carlson Mbah

Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Bamenda, Bamenda, Cameroon
Frank Eric Tatsing Foka

Department of Biological Sciences, Faculty of Agriculture, Science and Technology, North West University, Mafikeng, South Africa
Henri-Lucien Kamga

Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Bamenda, Bamenda, Cameroon

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Marie Ebob Agbortabot Bissong, Carlson Mbah, Frank Eric Tatsing Foka, Henri-Lucien Kamga. (2017). Spectrum of Uropathogens and Antimicrobial Susceptibility in Clinically Diagnosed Cases of Urinary Tract Infection in the Bamenda Regional Hospital, Cameroon. American Journal of Health Research, 5(2), 19-24. https://doi.org/10.11648/j.ajhr.20170502.11

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ACS Style

Marie Ebob Agbortabot Bissong; Carlson Mbah; Frank Eric Tatsing Foka; Henri-Lucien Kamga. Spectrum of Uropathogens and Antimicrobial Susceptibility in Clinically Diagnosed Cases of Urinary Tract Infection in the Bamenda Regional Hospital, Cameroon. Am. J. Health Res. 2017, 5(2), 19-24. doi: 10.11648/j.ajhr.20170502.11

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AMA Style

Marie Ebob Agbortabot Bissong, Carlson Mbah, Frank Eric Tatsing Foka, Henri-Lucien Kamga. Spectrum of Uropathogens and Antimicrobial Susceptibility in Clinically Diagnosed Cases of Urinary Tract Infection in the Bamenda Regional Hospital, Cameroon. Am J Health Res. 2017;5(2):19-24. doi: 10.11648/j.ajhr.20170502.11

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@article{10.11648/j.ajhr.20170502.11,
  author = {Marie Ebob Agbortabot Bissong and Carlson Mbah and Frank Eric Tatsing Foka and Henri-Lucien Kamga},
  title = {Spectrum of Uropathogens and Antimicrobial Susceptibility in Clinically Diagnosed Cases of Urinary Tract Infection in the Bamenda Regional Hospital, Cameroon},
  journal = {American Journal of Health Research},
  volume = {5},
  number = {2},
  pages = {19-24},
  doi = {10.11648/j.ajhr.20170502.11},
  url = {https://doi.org/10.11648/j.ajhr.20170502.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20170502.11},
  abstract = {Urinary tract infections (UTIs) are among the common bacterial infections in humans both in the community and hospital settings. In spite of the availability and use of antimicrobial drugs, UTIs have shown increasing trends in recent years. Antimicrobial resistance among uropathogens is a serious health threat as this may complicate treatment, eventually resulting to more severe disease. Despite the importance of UTI, there is paucity of information on the aetiology of this disease especially in the Northwest Region. For that reason, the present study was designed with the aim to determine the spectrum of uropathogens and antimicrobial resistance of isolates in cases of symptomatic bacteriuria. To achieve this goal, midstream urine specimens were inoculated unto CLED media and isolates were identified using the Enterosystem 18R. Significant bacteriuria was considered with bacterial growth of ≥105 CFU/ml. Susceptibility testing was performed using the Kirby Bauer disk diffusion method and the accuracy of the dipstick test was determined using culture as a gold standard. Significant bacteriuria was observed in 50% of the participants and the predominant uropathogens isolated were P. aeruginosa (22.4%), Klebsiella spp (14.3%), S. aureus (14.3%), CNS (14.3%) and E. coli (12.2%). High resistance (80-98%) among uropathogens was observed against clindamycin, amoxicillin and doxycycline. For the dipstick test, leucocyte esterase and nitrite showed high sensitivity (93.9%) and specificity (100%) respectively, for detecting UTI. The results of this study highlights the importance of Pseudomonas aeruginosa in the aetiology of both community and hospital acquired UTIs. In addition, gentamicin is the most effective agent against uropathogens in the study area.},
 year = {2017}
}

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