Anti-ulcerogenic Action Mechanism of <i>Maytenus ilicifolia</i> Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR

Dalva Pazzini; Elder Bruno Fontes; Ana Paula D. Hory; Adevailton B. Santos; Oswaldo Baffa; Rosângela Bezerra; Silvana Pasetto; Eduardo José Crevelin; Luiz Alberto Beraldo Moraes; Angela Kinoshita; Ana Cláudia B. Paula-Zurron

doi:doi:10.11648/j.ajhr.20170503.12

Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR

Dalva Pazzini, Elder Bruno Fontes, Ana Paula D. Hory, Adevailton B. Santos, Oswaldo Baffa, Rosângela Bezerra, Silvana Pasetto, Eduardo José Crevelin, Luiz Alberto Beraldo Moraes, Angela Kinoshita, Ana Cláudia B. Paula-Zurron

Published in American Journal of Health Research (Volume 5, Issue 3)

Received: 24 February 2017 Accepted: 24 March 2017 Published: 30 March 2017

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Abstract

Maytenus ilicifolia, known as “espinheira-santa”, is used in the popular medicine for peptic ulcer treatment. This work evaluates the preventive and curative actions of M. ilicifolia in animals, the healing activity by EGF expression detected by RT-PCR as well as the anti-oxidative activity by Electron Spin Resonance spectroscopy (ESR). Oral administration of M. ilicifolia (300 and 500 mg/kg, p.o.) one hour before the ulcerogenic agent application prevented the ulcer formation 42 ± 0.98% and 72 ± 1.2% (p <0.05 and p <0.001, respectively), in comparison with the negative control tween 80, preserving the cytoprotection characteristics of the gastric mucosa and assuring the integrity of gastric glands and gastric fossets. The animals treated with tween, had the epithelium and the mucosa layer damaged and accentuated vascularization. The healing activity of M. ilicifolia (500mg/kg, p.o.) was 71 ± 1.4% (p <0.001) in chronic ulcer experiments induced by acetic acid. EGF expression detected by RT-PCR confirmed the healing activity. Histological analysis showed the recovery of the mucosal layer and the epithelium surface harmed by the acetic acid. Studies in vitro by Electron Spin Resonance spectroscopy assessed the anti-oxidative action of M ilicifolia. Experiments with DPPH (2.2-Diphenyl -1-picrylhydrazyl) demonstrated that the polar fraction of M. ilicifolia presents an IC₅₀ of 0.680.09 mg/ml. The value of 92 mg/ml was found for IC₅₀ in experiments with the radical OH, produced by the Fenton Reaction and detected through spin adduct DMPO-OH. Mass spectrometry analysis of crude extracts and fractions from M. ilicifolia was carried out and compounds of pentacyclic triterpenes class were identified.

Published in	American Journal of Health Research (Volume 5, Issue 3)
DOI	10.11648/j.ajhr.20170503.12
Page(s)	57-64
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Maytenus ilicifolia, Anti-oxidative Activity, Antiulcerogenic Activity, Epidermal Growth Factor, Gastric Ulcer, Electron Spin Resonance

References

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APA Style

Dalva Pazzini, Elder Bruno Fontes, Ana Paula D. Hory, Adevailton B. Santos, Oswaldo Baffa, et al. (2017). Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR. American Journal of Health Research, 5(3), 57-64. https://doi.org/10.11648/j.ajhr.20170503.12

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ACS Style

Dalva Pazzini; Elder Bruno Fontes; Ana Paula D. Hory; Adevailton B. Santos; Oswaldo Baffa, et al. Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR. Am. J. Health Res. 2017, 5(3), 57-64. doi: 10.11648/j.ajhr.20170503.12

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AMA Style

Dalva Pazzini, Elder Bruno Fontes, Ana Paula D. Hory, Adevailton B. Santos, Oswaldo Baffa, et al. Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR. Am J Health Res. 2017;5(3):57-64. doi: 10.11648/j.ajhr.20170503.12

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@article{10.11648/j.ajhr.20170503.12,
  author = {Dalva Pazzini and Elder Bruno Fontes and Ana Paula D. Hory and Adevailton B. Santos and Oswaldo Baffa and Rosângela Bezerra and Silvana Pasetto and Eduardo José Crevelin and Luiz Alberto Beraldo Moraes and Angela Kinoshita and Ana Cláudia B. Paula-Zurron},
  title = {Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR},
  journal = {American Journal of Health Research},
  volume = {5},
  number = {3},
  pages = {57-64},
  doi = {10.11648/j.ajhr.20170503.12},
  url = {https://doi.org/10.11648/j.ajhr.20170503.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20170503.12},
  abstract = {Maytenus ilicifolia, known as “espinheira-santa”, is used in the popular medicine for peptic ulcer treatment. This work evaluates the preventive and curative actions of M. ilicifolia in animals, the healing activity by EGF expression detected by RT-PCR as well as the anti-oxidative activity by Electron Spin Resonance spectroscopy (ESR). Oral administration of M. ilicifolia (300 and 500 mg/kg, p.o.) one hour before the ulcerogenic agent application prevented the ulcer formation 42 ± 0.98% and 72 ± 1.2% (p M. ilicifolia (500mg/kg, p.o.) was 71 ± 1.4% (p in vitro by Electron Spin Resonance spectroscopy assessed the anti-oxidative action of M ilicifolia. Experiments with DPPH (2.2-Diphenyl -1-picrylhydrazyl) demonstrated that the polar fraction of M. ilicifolia presents an IC50 of 0.680.09 mg/ml. The value of 92 mg/ml was found for IC50 in experiments with the radical OH, produced by the Fenton Reaction and detected through spin adduct DMPO-OH. Mass spectrometry analysis of crude extracts and fractions from M. ilicifolia was carried out and compounds of pentacyclic triterpenes class were identified.},
 year = {2017}
}

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TY - JOUR
T1 - Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR
AU - Dalva Pazzini
AU - Elder Bruno Fontes
AU - Ana Paula D. Hory
AU - Adevailton B. Santos
AU - Oswaldo Baffa
AU - Rosângela Bezerra
AU - Silvana Pasetto
AU - Eduardo José Crevelin
AU - Luiz Alberto Beraldo Moraes
AU - Angela Kinoshita
AU - Ana Cláudia B. Paula-Zurron
Y1 - 2017/03/30
PY - 2017
N1 - https://doi.org/10.11648/j.ajhr.20170503.12
DO - 10.11648/j.ajhr.20170503.12
T2 - American Journal of Health Research
JF - American Journal of Health Research
JO - American Journal of Health Research
SP - 57
EP - 64
PB - Science Publishing Group
SN - 2330-8796
UR - https://doi.org/10.11648/j.ajhr.20170503.12
AB - Maytenus ilicifolia, known as “espinheira-santa”, is used in the popular medicine for peptic ulcer treatment. This work evaluates the preventive and curative actions of M. ilicifolia in animals, the healing activity by EGF expression detected by RT-PCR as well as the anti-oxidative activity by Electron Spin Resonance spectroscopy (ESR). Oral administration of M. ilicifolia (300 and 500 mg/kg, p.o.) one hour before the ulcerogenic agent application prevented the ulcer formation 42 ± 0.98% and 72 ± 1.2% (p M. ilicifolia (500mg/kg, p.o.) was 71 ± 1.4% (p in vitro by Electron Spin Resonance spectroscopy assessed the anti-oxidative action of M ilicifolia. Experiments with DPPH (2.2-Diphenyl -1-picrylhydrazyl) demonstrated that the polar fraction of M. ilicifolia presents an IC50 of 0.680.09 mg/ml. The value of 92 mg/ml was found for IC50 in experiments with the radical OH, produced by the Fenton Reaction and detected through spin adduct DMPO-OH. Mass spectrometry analysis of crude extracts and fractions from M. ilicifolia was carried out and compounds of pentacyclic triterpenes class were identified.
VL - 5
IS - 3
ER -

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Author Information

Dalva Pazzini

Pró-Reitoria de Pesquisa e Pós-Gradua??o, Universidade do Sagrado Cora??o – USC, Bauru, SP, Brazil
Elder Bruno Fontes

Pró-Reitoria de Pesquisa e Pós-Gradua??o, Universidade do Sagrado Cora??o – USC, Bauru, SP, Brazil
Ana Paula D. Hory

Pró-Reitoria de Pesquisa e Pós-Gradua??o, Universidade do Sagrado Cora??o – USC, Bauru, SP, Brazil
Adevailton B. Santos

Universidade Federal de Uberlandia, Uberlandia, MG, Brazil
Oswaldo Baffa

Departamento de Física, FFCLRP, Universidade de S?o Paulo- USP, Ribeir?o Preto, SP, Brazil
Rosângela Bezerra
Silvana Pasetto

Centro Universitário do Norte, UniNorte Manaus, AM, Brazil
Eduardo José Crevelin

Departamento de Química, FFCLRP, Universidade de S?o Paulo- USP, Ribeir?o Preto, SP, Brazil
Luiz Alberto Beraldo Moraes

Departamento de Química, FFCLRP, Universidade de S?o Paulo- USP, Ribeir?o Preto, SP, Brazil
Angela Kinoshita

Pró-Reitoria de Pesquisa e Pós-Gradua??o, Universidade do Sagrado Cora??o – USC, Bauru, SP, Brazil
Ana Cláudia B. Paula-Zurron

Kroton Educacional, Valinhos, SP, Brazil

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Plain Text BibTeX RIS

APA Style

Dalva Pazzini, Elder Bruno Fontes, Ana Paula D. Hory, Adevailton B. Santos, Oswaldo Baffa, et al. (2017). Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR. American Journal of Health Research, 5(3), 57-64. https://doi.org/10.11648/j.ajhr.20170503.12

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ACS Style

Dalva Pazzini; Elder Bruno Fontes; Ana Paula D. Hory; Adevailton B. Santos; Oswaldo Baffa, et al. Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR. Am. J. Health Res. 2017, 5(3), 57-64. doi: 10.11648/j.ajhr.20170503.12

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AMA Style

Dalva Pazzini, Elder Bruno Fontes, Ana Paula D. Hory, Adevailton B. Santos, Oswaldo Baffa, et al. Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR. Am J Health Res. 2017;5(3):57-64. doi: 10.11648/j.ajhr.20170503.12

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@article{10.11648/j.ajhr.20170503.12,
  author = {Dalva Pazzini and Elder Bruno Fontes and Ana Paula D. Hory and Adevailton B. Santos and Oswaldo Baffa and Rosângela Bezerra and Silvana Pasetto and Eduardo José Crevelin and Luiz Alberto Beraldo Moraes and Angela Kinoshita and Ana Cláudia B. Paula-Zurron},
  title = {Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR},
  journal = {American Journal of Health Research},
  volume = {5},
  number = {3},
  pages = {57-64},
  doi = {10.11648/j.ajhr.20170503.12},
  url = {https://doi.org/10.11648/j.ajhr.20170503.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20170503.12},
  abstract = {Maytenus ilicifolia, known as “espinheira-santa”, is used in the popular medicine for peptic ulcer treatment. This work evaluates the preventive and curative actions of M. ilicifolia in animals, the healing activity by EGF expression detected by RT-PCR as well as the anti-oxidative activity by Electron Spin Resonance spectroscopy (ESR). Oral administration of M. ilicifolia (300 and 500 mg/kg, p.o.) one hour before the ulcerogenic agent application prevented the ulcer formation 42 ± 0.98% and 72 ± 1.2% (p M. ilicifolia (500mg/kg, p.o.) was 71 ± 1.4% (p in vitro by Electron Spin Resonance spectroscopy assessed the anti-oxidative action of M ilicifolia. Experiments with DPPH (2.2-Diphenyl -1-picrylhydrazyl) demonstrated that the polar fraction of M. ilicifolia presents an IC50 of 0.680.09 mg/ml. The value of 92 mg/ml was found for IC50 in experiments with the radical OH, produced by the Fenton Reaction and detected through spin adduct DMPO-OH. Mass spectrometry analysis of crude extracts and fractions from M. ilicifolia was carried out and compounds of pentacyclic triterpenes class were identified.},
 year = {2017}
}

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